(Linear) (Six-base) MAPSORT of: KIEE3357.seq Check: 8643 from: 1 to: 3165 KIEE3357.orf With 182 enzymes: SgfI * July 3, 2012 17:45 .. AccI GT'mk_AC Cuts at: 0 2426 3165 Size: 2426 739 Acc65I G'GTAC_C Cuts at: 0 700 3165 Size: 700 2465 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 866 958 1339 1478 2588 3165 Size: 289 577 92 381 139 1110 577 Fragments arranged by size: 1110 577 577 381 289 139 92 AflII C'TTAA_G Cuts at: 0 745 3165 Size: 745 2420 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2984 3016 3165 Size: 2984 32 149 Fragments arranged by size: 2984 149 32 AlwNI CAG_nnn'CTG Cuts at: 0 1528 3165 Size: 1528 1637 ApoI r'AATT_y Cuts at: 0 574 651 740 937 1056 1132 1163 1180 Size: 574 77 89 197 119 76 31 17 Cuts at: 1180 1369 2496 2617 2658 3165 Size: 189 1127 121 41 507 Fragments arranged by size: 1127 574 507 197 189 121 119 89 77 76 41 31 17 AseI AT'TA_AT Cuts at: 0 89 1735 3165 Size: 89 1646 1430 Fragments arranged by size: 1646 1430 89 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 228 254 261 287 3165 Size: 228 26 7 26 2878 Fragments arranged by size: 2878 228 26 26 7 BanI G'GyrC_C Cuts at: 0 700 1605 2445 3165 Size: 700 905 840 720 Fragments arranged by size: 905 840 720 700 BanII G_rGCy'C Cuts at: 0 1488 3165 Size: 1488 1677 BbsI GAAGACnn'nnnn_ Cuts at: 0 1158 2078 2387 3165 Size: 1158 920 309 778 Fragments arranged by size: 1158 920 778 309 BbvCI CC'TCA_GC Cuts at: 0 185 673 3165 Size: 185 488 2492 Fragments arranged by size: 2492 488 185 BciVI GTATCCnnnnn_n' Cuts at: 0 1571 3165 Size: 1571 1594 BclI T'GATC_A Cuts at: 0 1839 3165 Size: 1839 1326 BfrBI ATG'CAT Cuts at: 0 1363 1599 3165 Size: 1363 236 1566 Fragments arranged by size: 1566 1363 236 BglII A'GATC_T Cuts at: 0 993 3165 Size: 993 2172 Bme1580I G_kGCm'C Cuts at: 0 1820 3165 Size: 1820 1345 BmrI ACTGGGnnnn_n' Cuts at: 0 1833 2021 3165 Size: 1833 188 1144 Fragments arranged by size: 1833 1144 188 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 479 3165 Size: 479 2686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 1568 1683 2855 3165 Size: 305 1263 115 1172 310 Fragments arranged by size: 1263 1172 310 305 115 Bpu10I CC'TnA_GC Cuts at: 0 185 673 3165 Size: 185 488 2492 Fragments arranged by size: 2492 488 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 342 602 1362 2525 3165 Size: 342 260 760 1163 640 Fragments arranged by size: 1163 760 640 342 260 BsaI GGTCTCn'nnnn_ Cuts at: 0 2201 3165 Size: 2201 964 BsaWI w'CCGG_w Cuts at: 0 1321 2046 3165 Size: 1321 725 1119 Fragments arranged by size: 1321 1119 725 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2984 3014 3165 Size: 2984 30 151 Fragments arranged by size: 2984 151 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 208 865 2008 2608 3165 Size: 208 657 1143 600 557 Fragments arranged by size: 1143 657 600 557 208 BseYI C'CCAG_C Cuts at: 0 1813 3165 Size: 1813 1352 BsmI GAATG_Cn' Cuts at: 0 177 346 3165 Size: 177 169 2819 Fragments arranged by size: 2819 177 169 BsmBI CGTCTCn'nnnn_ Cuts at: 0 847 2159 3165 Size: 847 1312 1006 Fragments arranged by size: 1312 1006 847 Bsp1286I G_dGCh'C Cuts at: 0 1488 1820 3165 Size: 1488 332 1345 Fragments arranged by size: 1488 1345 332 BspHI T'CATG_A Cuts at: 0 2870 3165 Size: 2870 295 BsrDI GCAATG_nn' Cuts at: 0 234 1025 3165 Size: 234 791 2140 Fragments arranged by size: 2140 791 234 BsrGI T'GTAC_A Cuts at: 0 1958 3165 Size: 1958 1207 BstAPI GCAn_nnn'nTGC Cuts at: 0 18 2119 3165 Size: 18 2101 1046 Fragments arranged by size: 2101 1046 18 BstBI TT'CG_AA Cuts at: 0 2828 3165 Size: 2828 337 BstYI r'GATC_y Cuts at: 0 993 3165 Size: 993 2172 BtgI C'CryG_G Cuts at: 0 5 881 3165 Size: 5 876 2284 Fragments arranged by size: 2284 876 5 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1642 3165 Size: 1642 1523 BtsI GCAGTG_nn' Cuts at: 0 155 3165 Size: 155 3010 ClaI AT'CG_AT Cuts at: 0 1773 2961 3165 Size: 1773 1188 204 Fragments arranged by size: 1773 1188 204 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2033 2068 3165 Size: 2033 35 1097 Fragments arranged by size: 2033 1097 35 DraIII CAC_nnn'GTG Cuts at: 0 1435 3165 Size: 1435 1730 EaeI y'GGCC_r Cuts at: 0 329 3165 Size: 329 2836 EarI CTCTTCn'nnn_ Cuts at: 0 427 842 857 1244 1895 2778 2830 3165 Size: 427 415 15 387 651 883 52 335 Fragments arranged by size: 883 651 427 415 387 335 52 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 305 866 958 1339 1478 1568 1683 Size: 289 16 561 92 381 139 90 115 Cuts at: 1683 2588 2855 3165 Size: 905 267 310 Fragments arranged by size: 905 561 381 310 289 267 139 115 92 90 16 EcoRI G'AATT_C Cuts at: 0 1056 3165 Size: 1056 2109 EcoRV GAT'ATC Cuts at: 0 2771 3165 Size: 2771 394 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 319 3165 Size: 319 2846 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2215 2247 3165 Size: 2215 32 918 Fragments arranged by size: 2215 918 32 HincII GTy'rAC Cuts at: 0 1109 1407 3165 Size: 1109 298 1758 Fragments arranged by size: 1758 1109 298 HindIII A'AGCT_T Cuts at: 0 2129 2143 3165 Size: 2129 14 1022 Fragments arranged by size: 2129 1022 14 HpaI GTT'AAC Cuts at: 0 1109 3165 Size: 1109 2056 KpnI G_GTAC'C Cuts at: 0 704 3165 Size: 704 2461 MfeI C'AATT_G Cuts at: 0 314 1727 3165 Size: 314 1413 1438 Fragments arranged by size: 1438 1413 314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2327 3165 Size: 2327 838 MslI CAynn'nnrTG Cuts at: 0 244 952 2300 3165 Size: 244 708 1348 865 Fragments arranged by size: 1348 865 708 244 NcoI C'CATG_G Cuts at: 0 881 3165 Size: 881 2284 NsiI A_TGCA'T Cuts at: 0 1365 1601 3165 Size: 1365 236 1564 Fragments arranged by size: 1564 1365 236 NspI r_CATG'y Cuts at: 0 428 1215 1783 3165 Size: 428 787 568 1382 Fragments arranged by size: 1382 787 568 428 PflMI CCAn_nnn'nTGG Cuts at: 0 1435 3165 Size: 1435 1730 PfoI T'CCnGG_A Cuts at: 0 1573 3165 Size: 1573 1592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2984 3016 3165 Size: 2984 32 149 Fragments arranged by size: 2984 149 32 PstI C_TGCA'G Cuts at: 0 26 3165 Size: 26 3139 SapI GCTCTTCn'nnn_ Cuts at: 0 1895 3165 Size: 1895 1270 ScaI AGT'ACT Cuts at: 0 923 3165 Size: 923 2242 SfcI C'TryA_G Cuts at: 0 22 106 443 2942 3165 Size: 22 84 337 2499 223 Fragments arranged by size: 2499 337 223 84 22 SmlI C'TyrA_G Cuts at: 0 321 617 745 1377 2540 3165 Size: 321 296 128 632 1163 625 Fragments arranged by size: 1163 632 625 321 296 128 StyI C'CwwG_G Cuts at: 0 881 1340 1609 2285 2976 3165 Size: 881 459 269 676 691 189 Fragments arranged by size: 881 691 676 459 269 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 112 3165 Size: 112 3053 TatI w'GTAC_w Cuts at: 0 921 1739 1865 1958 3165 Size: 921 818 126 93 1207 Fragments arranged by size: 1207 921 818 126 93 XbaI T'CTAG_A Cuts at: 0 2220 3165 Size: 2220 945 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1810 3165 Size: 1810 1355 XmnI GAAnn'nnTTC Cuts at: 0 1163 2270 2660 3165 Size: 1163 1107 390 505 Fragments arranged by size: 1163 1107 505 390 Enzymes that do cut: AccI Acc65I AcuI AflII AloI AlwNI ApoI AseI BaeI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspHI BsrDI BsrGI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EarI Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII HpaI KpnI MfeI MmeI MslI NcoI NsiI NspI PflMI PfoI PpiI PstI SapI ScaI SfcI SmlI StyI TaqII TatI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BcgI BglI BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BstZ17I Bsu36I DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII KasI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XhoI XmaI ZraI