Restriction map for
pFN21AE0995
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BciVI BfrI BlnI
BlpI Bpu1102I BpuAI BsiWI BsmBI
Bsp1407I BspDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 1126 CGCG CG^CG
AccIII 1907 TCCGGA T^CCGGA
AcsI 1568 AAATTC [GA]^AATT[CT]
AcyI 1321 GACGCC G[GA]^CG[CT]C
AflIII 1604 ACATGT A^C[GA][CT]GT
Aor51HI 1799 AGCGCT AGC^GCT
ApoI 1568 AAATTC [GA]^AATT[CT]
Asp700I 742 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 1385 GCAAAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 999 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1321 GACGCC G[GA]^CG[CT]C
BsaWI 1907 TCCGGA [AT]^CCGG[AT]
BseAI 1907 TCCGGA T^CCGGA
BsgI 1300 GTGCAG GTGCAG(16/14)
BsiEI 1014 CGGTCG CG[GA][CT]^CG
BsmI 29 GCATTC GAATGC(1/-1)
BspEI 1907 TCCGGA T^CCGGA
BspHI 1367 TCATGA T^CATGA
BspLU11I 1604 ACATGT A^CATGT
BsrBI 1003 GAGCGG CCGCTC(-3/-3)
BsrDI 1041 CATTGC GCAATG(2/0)
BsrFI 1772 GCCGGT [GA]^CCGG[CT]
BstAPI 565 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1126 CGCG CG^CG
CfoI 1800 GCGC GCG^C
Cfr10I 1772 GCCGGT [GA]^CCGG[CT]
EaeI 1074 TGGCCG [CT]^GGCC[GA]
Eco47III 1799 AGCGCT AGC^GCT
FauI 844 GCGGG CCCGC(4/6)
HaeII 1799 AGCGCT [GA]GCGC^[CT]
HgaI 1321 GACGC GACGC(5/10)
HhaI 1800 GCGC GCG^C
Hin1I 1321 GACGCC G[GA]^CG[CT]C
HinP1I 1800 GCGC G^CGC
MroI 1907 TCCGGA T^CCGGA
MseI 1691 TTAA T^TAA
MvnI 1126 CGCG CG^CG
NcoI 1031 CCATGG C^CATGG
RcaI 1367 TCATGA T^CATGA
SacI 1684 GAGCTC GAGCT^C
SapI 24 GAAGAGC GCTCTTC(1/4)
SmaI 68 CCCGGG CCC^GGG
SnaBI 1555 TACGTA TAC^GTA
StuI 1139 AGGCCT AGG^CCT
TaqI 1838 TCGA T^CGA
TfiI 624 GAATC G^A[AT]TC
Tru9I 1691 TTAA T^TAA
TthHB8I 1838 TCGA T^CGA
XcmI 650 CCAGCAAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
XmnI 742 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1258 GTCTAC GT^[AC][GT]AC
1786 GTAGAC
AfaI 54 GTAC GT^AC
1296 GTAC
AlwNI 448 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
540 CAGAAGCTG
ApaI 71 GGGCCC GGGCC^C
504 GGGCCC
AspHI 835 GAGCAC G[AT]GC[AT]^C
1684 GAGCTC
BanI 221 GGCACC G^G[CT][GA]CC
1121 GGCACC
BclI 1211 TGATCA T^GATCA
1370 TGATCA
BfaI 253 CTAG C^TAG
961 CTAG
BglII 1205 AGATCT A^GATCT
1761 AGATCT
BsaAI 1297 TACGTG [CT]AC^GT[GA]
1555 TACGTA
BsaBI 1149 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1206 GATCTTGATC
BsiHKAI 835 GAGCAC G[AT]GC[AT]^C
1684 GAGCTC
BstXI 153 CCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
482 CCAGTGAGCTGG
DsaI 1024 CCACGG C^C[GA][CT]GG
1031 CCATGG
FbaI 1211 TGATCA T^GATCA
1370 TGATCA
MaeI 253 CTAG C^TAG
961 CTAG
MaeII 1298 ACGT A^CGT
1556 ACGT
MamI 1149 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1206 GATCTTGATC
PleI 933 GACTC GAGTC(4/5)
1172 GACTC
PstI 186 CTGCAG CTGCA^G
546 CTGCAG
PvuII 1702 CAGCTG CAG^CTG
1844 CAGCTG
RsaI 54 GTAC GT^AC
1296 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbvCI BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I Tsp509I
TspRI XhoII