(Circular) (Six-base) MAPSORT of: pFN21AE0995.seq Check: 8683 from: 1 to: 6649 pFN21AE0995 6649 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3874 3874 Size: 6649 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4459 278 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3264 3792 3992 1937 Size: 1327 528 200 4594 Fragments arranged by size: 4594 1327 528 200 Acc65I G'GTAC_C Cuts at: 3970 3970 Size: 6649 AclI AA'CG_TT Cuts at: 1563 4776 5149 1563 Size: 3213 373 3063 Fragments arranged by size: 3213 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2432 3812 4712 6096 6364 1632 Size: 416 384 1380 900 1384 268 1917 Fragments arranged by size: 1917 1384 1380 900 416 384 268 AfeI AGC'GCT Cuts at: 3806 3806 Size: 6649 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6452 AflIII A'CryG_T Cuts at: 1203 3609 5548 6525 1203 Size: 2406 1939 977 1327 Fragments arranged by size: 2406 1939 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 5378 5378 Size: 6649 AleI CACnn'nnGTG Cuts at: 1166 2100 2163 1166 Size: 934 63 5652 Fragments arranged by size: 5652 934 63 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2118 3165 1184 Size: 934 1047 4668 Fragments arranged by size: 4668 1047 934 AlwNI CAG_nnn'CTG Cuts at: 2458 2550 4392 5964 2458 Size: 92 1842 1572 3143 Fragments arranged by size: 3143 1842 1572 92 ApaI G_GGCC'C Cuts at: 2080 2513 2080 Size: 433 6216 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 3573 3958 4184 4336 6445 1493 Size: 2080 385 226 152 2109 1697 Fragments arranged by size: 2109 2080 1697 385 226 152 AseI AT'TA_AT Cuts at: 160 1032 5203 160 Size: 872 4171 1606 Fragments arranged by size: 4171 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2243 2401 2564 2874 3974 1870 Size: 73 130 170 158 163 310 1100 4545 Fragments arranged by size: 4545 1100 310 170 163 158 130 73 BamHI G'GATC_C Cuts at: 3979 4346 3979 Size: 367 6282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2226 3126 3970 5425 618 Size: 325 217 627 439 900 844 1455 1842 Fragments arranged by size: 1842 1455 900 844 627 439 325 217 BanII G_rGCy'C Cuts at: 1391 2080 2513 2979 3360 3693 3968 1391 Size: 689 433 466 381 333 275 4072 Fragments arranged by size: 4072 689 466 433 381 333 275 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5678 BbvCI CC'TCA_GC Cuts at: 2120 2645 2774 2120 Size: 525 129 5995 Fragments arranged by size: 5995 525 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3379 3413 4840 4874 1903 Size: 34 1442 34 1427 34 3678 Fragments arranged by size: 3678 1442 1427 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4541 5751 4541 Size: 1210 5439 BclI T'GATC_A Cuts at: 1548 3216 3375 1548 Size: 1668 159 4822 Fragments arranged by size: 4822 1668 159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3010 5260 136 Size: 107 122 71 631 605 1338 2250 1525 Fragments arranged by size: 2250 1525 1338 631 605 122 107 71 BglII A'GATC_T Cuts at: 3210 3766 6644 3210 Size: 556 2878 3215 Fragments arranged by size: 3215 2878 556 BlpI GC'TnA_GC Cuts at: 4066 4066 Size: 6649 Bme1580I G_kGCm'C Cuts at: 1790 2080 2107 2513 4710 5866 1790 Size: 290 27 406 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 406 290 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6649 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2310 3748 4380 Size: 416 53 83 399 24 893 1438 632 Cuts at: 4380 5338 442 Size: 958 1753 Fragments arranged by size: 1753 1438 958 893 632 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2493 3249 2493 Size: 756 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 2943 3317 3620 5309 1239 Size: 1241 463 374 303 1689 2579 Fragments arranged by size: 2579 1689 1241 463 374 303 Bpu10I CC'TnA_GC Cuts at: 2120 2645 2712 2774 2120 Size: 525 67 62 5995 Fragments arranged by size: 5995 525 67 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2622 3164 3422 4774 5639 5937 2622 Size: 542 258 1352 865 298 3334 Fragments arranged by size: 3334 1352 865 542 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2148 2165 2171 2565 2644 3128 3309 Size: 617 649 17 6 394 79 484 181 Cuts at: 3309 3520 5312 882 Size: 211 1792 2219 Fragments arranged by size: 2219 1792 649 617 484 394 211 181 79 17 6 BsaAI yAC'GTr Cuts at: 493 1204 3304 3562 4440 493 Size: 711 2100 258 878 2702 Fragments arranged by size: 2702 2100 878 711 258 BsaBI GATnn'nnATC Cuts at: 3158 3215 6643 3158 Size: 57 3428 3164 Fragments arranged by size: 3428 3164 57 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3327 4456 4838 5473 Size: 53 83 186 936 1794 1129 382 635 Cuts at: 5473 275 Size: 1451 Fragments arranged by size: 1794 1451 1129 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3912 5082 5480 5754 5901 6312 3912 Size: 1170 398 274 147 411 4249 Fragments arranged by size: 4249 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 1178 Size: 30 165 30 1176 30 5218 Fragments arranged by size: 5218 1176 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2137 2261 2662 2780 2892 3327 1186 Size: 951 124 401 118 112 435 4508 Fragments arranged by size: 4508 951 435 401 124 118 112 BseYI C'CCAG_C Cuts at: 2091 2654 3014 3709 3893 5852 6348 2091 Size: 563 360 695 184 1959 496 2392 Fragments arranged by size: 2392 1959 695 563 496 360 184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3326 1163 Size: 573 1590 4486 Fragments arranged by size: 4486 1590 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3022 4099 4860 5888 1540 Size: 275 186 1021 1077 761 1028 2301 Fragments arranged by size: 2301 1077 1028 1021 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2844 3693 4710 4795 5866 2844 Size: 849 1017 85 1071 3627 Fragments arranged by size: 3627 1071 1017 849 85 BsmI GAATG_Cn' Cuts at: 2034 4160 4253 2034 Size: 2126 93 4430 Fragments arranged by size: 4430 2126 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2107 2513 2844 2979 3360 3693 Size: 399 290 27 406 331 135 381 333 Cuts at: 3693 3968 4710 4795 5866 1391 Size: 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 381 333 331 290 275 135 85 27 BspEI T'CCGG_A Cuts at: 3912 6312 3912 Size: 2400 4249 BspHI T'CATG_A Cuts at: 3372 4538 6268 3372 Size: 1166 1730 3753 Fragments arranged by size: 3753 1730 1166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2965 3874 3931 4004 844 Size: 2121 909 57 73 3489 Fragments arranged by size: 3489 2121 909 73 57 BsrBI CCG'CTC Cuts at: 1394 1823 3010 4536 1394 Size: 429 1187 1526 3507 Fragments arranged by size: 3507 1526 1187 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3045 5144 5318 65 Size: 1183 1797 2099 174 1396 Fragments arranged by size: 2099 1797 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3777 5293 5480 1700 Size: 110 1967 1516 187 2869 Fragments arranged by size: 2869 1967 1516 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 4709 5721 4709 Size: 1012 5637 BstAPI GCAn_nnn'nTGC Cuts at: 794 2576 794 Size: 1782 4867 BstBI TT'CG_AA Cuts at: 5453 5453 Size: 6649 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6649 BstXI CCAn_nnnn'nTGG Cuts at: 2165 2494 2165 Size: 329 6320 BstYI r'GATC_y Cuts at: 1972 2238 3210 3766 3979 4346 4731 4748 6200 Size: 266 972 556 213 367 385 17 1452 Cuts at: 6200 6286 6298 6644 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 972 556 385 367 346 266 213 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3029 3036 513 Size: 552 939 1025 7 4126 Fragments arranged by size: 4126 1025 939 552 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3167 487 Size: 683 56 1941 3969 Fragments arranged by size: 3969 1941 683 56 BtsI GCAGTG_nn' Cuts at: 4165 4959 4979 4165 Size: 794 20 5835 Fragments arranged by size: 5835 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2803 2838 3501 3536 529 Size: 35 2239 35 663 35 3642 Fragments arranged by size: 3642 2239 663 35 35 35 DraI TTT'AAA Cuts at: 3953 3953 Size: 6649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5656 809 Size: 507 4340 1802 Fragments arranged by size: 4340 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3079 4096 4985 Size: 54 1425 50 275 18 1249 1017 889 Cuts at: 4985 6614 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1249 1017 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2343 2877 2988 4579 1353 Size: 671 319 534 111 1591 3423 Fragments arranged by size: 3423 1591 671 534 319 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5236 5610 5756 387 Size: 1064 138 3647 374 146 1280 Fragments arranged by size: 3647 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3691 3691 Size: 6649 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2432 2480 2943 3317 3620 3812 Size: 393 416 384 48 463 374 303 192 Cuts at: 3812 4712 5309 6096 6364 1239 Size: 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 597 463 416 393 384 374 303 268 192 48 EcoO109I rG'GnC_Cy Cuts at: 2077 2274 2509 2737 2871 3433 3549 2077 Size: 197 235 228 134 562 116 5177 Fragments arranged by size: 5177 562 235 228 197 134 116 EcoRI G'AATT_C Cuts at: 3958 4336 3958 Size: 378 6271 FspI TGC'GCA Cuts at: 5155 5155 Size: 6649 HaeII r_GCGC'y Cuts at: 1678 3808 5796 1678 Size: 2130 1988 2531 Fragments arranged by size: 2531 2130 1988 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3461 3493 3734 3766 3929 Size: 32 135 32 1889 32 241 32 163 Cuts at: 3929 3961 5290 5322 5364 5396 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1889 1329 241 163 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3993 4239 669 Size: 988 281 2055 246 3079 Fragments arranged by size: 3079 2055 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6649 HpaI GTT'AAC Cuts at: 4239 4239 Size: 6649 KpnI G_GTAC'C Cuts at: 3974 3974 Size: 6649 MfeI C'AATT_G Cuts at: 4248 4248 Size: 6649 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3002 5763 5947 1305 Size: 1697 2761 184 2007 Fragments arranged by size: 2761 2007 1697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4827 Fragments arranged by size: 4827 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2100 2163 3377 4607 4966 5125 Size: 648 873 61 63 1214 1230 359 159 Cuts at: 5125 518 Size: 2042 Fragments arranged by size: 2042 1230 1214 873 648 359 159 63 61 MspA1I CmG'CkG Cuts at: 1606 2163 3018 3709 3851 4065 4742 5890 6135 Size: 557 855 691 142 214 677 1148 245 Cuts at: 6135 1606 Size: 2120 Fragments arranged by size: 2120 1148 855 691 677 557 245 214 142 NcoI C'CATG_G Cuts at: 513 1065 2004 3036 513 Size: 552 939 1032 4126 Fragments arranged by size: 4126 1032 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6649 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 3409 3521 3613 4007 5552 1132 Size: 97 2180 112 92 394 1545 2229 Fragments arranged by size: 2229 2180 1545 394 112 97 92 PasI CC'CwG_GG Cuts at: 1348 2331 1348 Size: 983 5666 PciI A'CATG_T Cuts at: 3609 5548 3609 Size: 1939 4710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6649 PfoI T'CCnGG_A Cuts at: 3294 3351 3294 Size: 57 6592 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6231 6263 4718 Size: 32 1481 32 5104 Fragments arranged by size: 5104 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2274 2737 2871 3433 3549 2274 Size: 463 134 562 116 5374 Fragments arranged by size: 5374 562 463 134 116 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6649 PsiI TTA'TAA Cuts at: 4219 4219 Size: 6649 PspOMI G'GGCC_C Cuts at: 2076 2509 2076 Size: 433 6216 PstI C_TGCA'G Cuts at: 830 2195 2555 4001 830 Size: 1365 360 1446 3478 Fragments arranged by size: 3478 1446 1365 360 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 PvuII CAG'CTG Cuts at: 3709 3851 3709 Size: 142 6507 SacI G_AGCT'C Cuts at: 3693 3693 Size: 6649 SalI G'TCGA_C Cuts at: 1936 3991 1936 Size: 2055 4594 SanDI GG'GwC_CC Cuts at: 3549 3549 Size: 6649 SapI GCTCTTCn'nnn_ Cuts at: 2024 2024 Size: 6649 SbfI CC_TGCA'GG Cuts at: 4001 4001 Size: 6649 ScaI AGT'ACT Cuts at: 4897 6609 4897 Size: 1712 4937 SfcI C'TryA_G Cuts at: 826 1045 2046 2191 2551 3997 5132 5813 6004 Size: 219 1001 145 360 1446 1135 681 191 Cuts at: 6004 826 Size: 1471 Fragments arranged by size: 1471 1446 1135 1001 681 360 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 2075 3976 1872 Size: 203 1901 4545 Fragments arranged by size: 4545 1901 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 2637 3179 3437 4753 5654 5916 Size: 197 926 694 542 258 1316 901 262 Cuts at: 5916 820 Size: 1553 Fragments arranged by size: 1553 1316 926 901 694 542 262 258 197 SnaBI TAC'GTA Cuts at: 493 3562 4440 493 Size: 3069 878 2702 Fragments arranged by size: 3069 2702 878 SpeI A'CTAG_T Cuts at: 152 152 Size: 6649 SphI G_CATG'C Cuts at: 1132 4007 1132 Size: 2875 3774 SrfI GCCC'GGGC Cuts at: 2075 2075 Size: 6649 SspI AAT'ATT Cuts at: 5 52 4573 5 Size: 47 4521 2081 Fragments arranged by size: 4521 2081 47 StuI AGG'CCT Cuts at: 1517 3146 1517 Size: 1629 5020 StyI C'CwwG_G Cuts at: 513 1065 2004 2114 2724 3036 4088 513 Size: 552 939 110 610 312 1052 3074 Fragments arranged by size: 3074 1052 939 610 552 312 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 4676 4693 4846 5031 5476 325 Size: 1255 609 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4895 6607 96 Size: 274 80 33 51 1443 2918 1712 138 Fragments arranged by size: 2918 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 3588 3620 6609 6641 2579 Size: 32 977 32 2989 32 2587 Fragments arranged by size: 2989 2587 977 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6649 XbaI T'CTAG_A Cuts at: 3985 3985 Size: 6649 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2662 2662 Size: 6649 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 2073 3974 1870 Size: 203 1901 4545 Fragments arranged by size: 4545 1901 203 XmnI GAAnn'nnTTC Cuts at: 2751 4778 2751 Size: 2027 4622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4457 276 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SwaI