(Linear) (Six-base) MAPSORT of: KIEE0995.seq Check: 2735 from: 1 to: 1944 KIEE0995.orf With 182 enzymes: SgfI * July 3, 2012 16:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1869 1944 Size: 1869 75 AccI GT'mk_AC Cuts at: 0 1259 1787 1944 Size: 1259 528 157 Fragments arranged by size: 1259 528 157 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 427 1807 1944 Size: 43 384 1380 137 Fragments arranged by size: 1380 384 137 43 AfeI AGC'GCT Cuts at: 0 1801 1944 Size: 1801 143 AflIII A'CryG_T Cuts at: 0 1604 1944 Size: 1604 340 AleI CACnn'nnGTG Cuts at: 0 95 158 1944 Size: 95 63 1786 Fragments arranged by size: 1786 95 63 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 113 1160 1944 Size: 113 1047 784 Fragments arranged by size: 1047 784 113 AlwNI CAG_nnn'CTG Cuts at: 0 453 545 1944 Size: 453 92 1399 Fragments arranged by size: 1399 453 92 ApaI G_GGCC'C Cuts at: 0 75 508 1944 Size: 75 433 1436 Fragments arranged by size: 1436 433 75 ApoI r'AATT_y Cuts at: 0 1568 1944 Size: 1568 376 AvaI C'yCGr_G Cuts at: 0 68 238 396 559 869 1944 Size: 68 170 158 163 310 1075 Fragments arranged by size: 1075 310 170 163 158 68 BanI G'GyrC_C Cuts at: 0 221 1121 1944 Size: 221 900 823 Fragments arranged by size: 900 823 221 BanII G_rGCy'C Cuts at: 0 75 508 974 1355 1688 1944 Size: 75 433 466 381 333 256 Fragments arranged by size: 466 433 381 333 256 75 BbvCI CC'TCA_GC Cuts at: 0 115 640 769 1944 Size: 115 525 129 1175 Fragments arranged by size: 1175 525 129 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1374 1408 1944 Size: 1374 34 536 Fragments arranged by size: 1374 536 34 BclI T'GATC_A Cuts at: 0 1211 1370 1944 Size: 1211 159 574 Fragments arranged by size: 1211 574 159 BglI GCCn_nnn'nGGC Cuts at: 0 1005 1944 Size: 1005 939 BglII A'GATC_T Cuts at: 0 1205 1761 1944 Size: 1205 556 183 Fragments arranged by size: 1205 556 183 Bme1580I G_kGCm'C Cuts at: 0 75 102 508 1944 Size: 75 27 406 1436 Fragments arranged by size: 1436 406 75 27 BmrI ACTGGGnnnn_n' Cuts at: 0 305 1743 1944 Size: 305 1438 201 Fragments arranged by size: 1438 305 201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 488 1244 1944 Size: 488 756 700 Fragments arranged by size: 756 700 488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 938 1312 1615 1944 Size: 475 463 374 303 329 Fragments arranged by size: 475 463 374 329 303 Bpu10I CC'TnA_GC Cuts at: 0 115 640 707 769 1944 Size: 115 525 67 62 1175 Fragments arranged by size: 1175 525 115 67 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 617 1159 1417 1944 Size: 617 542 258 527 Fragments arranged by size: 617 542 527 258 BsaI GGTCTCn'nnnn_ Cuts at: 0 143 160 166 560 639 1123 1304 1515 Size: 143 17 6 394 79 484 181 211 Cuts at: 1515 1944 Size: 429 Fragments arranged by size: 484 429 394 211 181 143 79 17 6 BsaAI yAC'GTr Cuts at: 0 1299 1557 1944 Size: 1299 258 387 Fragments arranged by size: 1299 387 258 BsaBI GATnn'nnATC Cuts at: 0 1153 1210 1944 Size: 1153 57 734 Fragments arranged by size: 1153 734 57 BsaHI Gr'CG_yC Cuts at: 0 1322 1944 Size: 1322 622 BsaWI w'CCGG_w Cuts at: 0 1907 1944 Size: 1907 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 574 604 1944 Size: 574 30 1340 Fragments arranged by size: 1340 574 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 132 256 657 775 887 1322 1944 Size: 132 124 401 118 112 435 622 Fragments arranged by size: 622 435 401 132 124 118 112 BseYI C'CCAG_C Cuts at: 0 86 649 1009 1704 1888 1944 Size: 86 563 360 695 184 56 Fragments arranged by size: 695 563 360 184 86 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1321 1944 Size: 1321 623 BsiEI CG_ry'CG Cuts at: 0 1017 1944 Size: 1017 927 BsiHKAI G_wGCw'C Cuts at: 0 839 1688 1944 Size: 839 849 256 Fragments arranged by size: 849 839 256 BsmI GAATG_Cn' Cuts at: 0 29 1944 Size: 29 1915 Bsp1286I G_dGCh'C Cuts at: 0 75 102 508 839 974 1355 1688 1944 Size: 75 27 406 331 135 381 333 256 Fragments arranged by size: 406 381 333 331 256 135 75 27 BspEI T'CCGG_A Cuts at: 0 1907 1944 Size: 1907 37 BspHI T'CATG_A Cuts at: 0 1367 1944 Size: 1367 577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 960 1869 1926 1944 Size: 960 909 57 18 Fragments arranged by size: 960 909 57 18 BsrBI CCG'CTC Cuts at: 0 1005 1944 Size: 1005 939 BsrDI GCAATG_nn' Cuts at: 0 1040 1944 Size: 1040 904 BsrFI r'CCGG_y Cuts at: 0 1772 1944 Size: 1772 172 BstAPI GCAn_nnn'nTGC Cuts at: 0 571 1944 Size: 571 1373 BstXI CCAn_nnnn'nTGG Cuts at: 0 160 489 1944 Size: 160 329 1455 Fragments arranged by size: 1455 329 160 BstYI r'GATC_y Cuts at: 0 233 1205 1761 1944 Size: 233 972 556 183 Fragments arranged by size: 972 556 233 183 BtgI C'CryG_G Cuts at: 0 1024 1031 1944 Size: 1024 7 913 Fragments arranged by size: 1024 913 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1162 1944 Size: 1162 782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 798 833 1496 1531 1944 Size: 798 35 663 35 413 Fragments arranged by size: 798 663 413 35 35 EaeI y'GGCC_r Cuts at: 0 1074 1944 Size: 1074 870 EarI CTCTTCn'nnn_ Cuts at: 0 19 338 872 983 1944 Size: 19 319 534 111 961 Fragments arranged by size: 961 534 319 111 19 EcoICRI GAG'CTC Cuts at: 0 1686 1944 Size: 1686 258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 427 475 938 1312 1615 1807 1944 Size: 43 384 48 463 374 303 192 137 Fragments arranged by size: 463 384 374 303 192 137 48 43 EcoO109I rG'GnC_Cy Cuts at: 0 72 269 504 732 866 1428 1544 1944 Size: 72 197 235 228 134 562 116 400 Fragments arranged by size: 562 400 235 228 197 134 116 72 HaeII r_GCGC'y Cuts at: 0 1803 1944 Size: 1803 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1456 1488 1729 1761 1924 1944 Size: 1456 32 241 32 163 20 Fragments arranged by size: 1456 241 163 32 32 20 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 997 1944 Size: 997 947 MslI CAynn'nnrTG Cuts at: 0 34 95 158 1372 1944 Size: 34 61 63 1214 572 Fragments arranged by size: 1214 572 63 61 34 MspA1I CmG'CkG Cuts at: 0 158 1013 1704 1846 1944 Size: 158 855 691 142 98 Fragments arranged by size: 855 691 158 142 98 NcoI C'CATG_G Cuts at: 0 1031 1944 Size: 1031 913 NspI r_CATG'y Cuts at: 0 1404 1516 1608 1944 Size: 1404 112 92 336 Fragments arranged by size: 1404 336 112 92 PasI CC'CwG_GG Cuts at: 0 326 1944 Size: 326 1618 PciI A'CATG_T Cuts at: 0 1604 1944 Size: 1604 340 PfoI T'CCnGG_A Cuts at: 0 1289 1346 1944 Size: 1289 57 598 Fragments arranged by size: 1289 598 57 PpuMI rG'GwC_Cy Cuts at: 0 269 732 866 1428 1544 1944 Size: 269 463 134 562 116 400 Fragments arranged by size: 562 463 400 269 134 116 PspOMI G'GGCC_C Cuts at: 0 71 504 1944 Size: 71 433 1440 Fragments arranged by size: 1440 433 71 PstI C_TGCA'G Cuts at: 0 190 550 1944 Size: 190 360 1394 Fragments arranged by size: 1394 360 190 PvuII CAG'CTG Cuts at: 0 1704 1846 1944 Size: 1704 142 98 Fragments arranged by size: 1704 142 98 SacI G_AGCT'C Cuts at: 0 1688 1944 Size: 1688 256 SanDI GG'GwC_CC Cuts at: 0 1544 1944 Size: 1544 400 SapI GCTCTTCn'nnn_ Cuts at: 0 19 1944 Size: 19 1925 SfcI C'TryA_G Cuts at: 0 41 186 546 1944 Size: 41 145 360 1398 Fragments arranged by size: 1398 360 145 41 SmaI CCC'GGG Cuts at: 0 70 1944 Size: 70 1874 SmlI C'TyrA_G Cuts at: 0 632 1174 1432 1944 Size: 632 542 258 512 Fragments arranged by size: 632 542 512 258 SnaBI TAC'GTA Cuts at: 0 1557 1944 Size: 1557 387 SrfI GCCC'GGGC Cuts at: 0 70 1944 Size: 70 1874 StuI AGG'CCT Cuts at: 0 1141 1944 Size: 1141 803 StyI C'CwwG_G Cuts at: 0 109 719 1031 1944 Size: 109 610 312 913 Fragments arranged by size: 913 610 312 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 184 1944 Size: 184 1760 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 574 606 1583 1615 1944 Size: 574 32 977 32 329 Fragments arranged by size: 977 574 329 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 657 1944 Size: 657 1287 XmaI C'CCGG_G Cuts at: 0 68 1944 Size: 68 1876 XmnI GAAnn'nnTTC Cuts at: 0 746 1944 Size: 746 1198 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbvCI BcgI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MslI MspA1I NcoI NspI PasI PciI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI SfcI SmaI SmlI SnaBI SrfI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BfrBI BlpI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI ZraI