Restriction map for
pFN21AE3393
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiEI BsiWI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI CelII Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KspI MluI MroI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 3166 GGTACC G^GTACC
AlwNI 3500 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3243 ATTAAT AT^TAAT
AsnI 3243 ATTAAT AT^TAAT
Asp718I 3166 GGTACC G^GTACC
AspHI 721 GAGCTC G[AT]GC[AT]^C
AvaI 1421 CTCGAG C^[CT]CG[GA]G
BalI 2436 TGGCCA TGG^CCA
BanII 721 GAGCTC G[GA]GC[CT]^C
BcgI 42 CGATGTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 570 GTATCC GTATCC(6/5)
BmyI 721 GAGCTC G[AGT]GC[ACT]^C
BsaBI 1018 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3115 GGTCTC GGTCTC(1/5)
BsaWI 711 ACCGGA [AT]^CCGG[AT]
BsgI 2219 CTGCAC GTGCAG(16/14)
BsiHKAI 721 GAGCTC G[AT]GC[AT]^C
BsoBI 1421 CTCGAG C^[CT]CG[GA]G
Bsp1286I 721 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 3183 TGTACA T^GTACA
BspDI 1351 ATCGAT AT^CGAT
BspMI 2976 ACCTGC ACCTGC(4/8)
BsrDI 2842 GCAATG GCAATG(2/0)
BsrGI 3183 TGTACA T^GTACA
BssKI 736 CCAGG ^CC[ACGT]GG
Bst1107I 264 GTATAC GTA^TAC
BstNI 736 CCAGG CC^[AT]GG
BstXI 140 CCACACAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 264 GTATAC GTA^TAC
CfoI 501 GCGC GCG^C
ClaI 1351 ATCGAT AT^CGAT
DraI 2010 TTTAAA TTT^AAA
DraII 171 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 269 CCGTGG C^C[GA][CT]GG
EaeI 2436 TGGCCA [CT]^GGCC[GA]
EcoNI 123 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 171 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1131 GAATTC G^AATTC
EcoRII 736 CCAGG ^CC[AT]GG
EcoT22I 2760 ATGCAT ATGCA^T
FauI 3472 GCGGG CCCGC(4/6)
HaeII 500 AGCGCC [GA]GCGC^[CT]
HapII 712 CCGG C^CGG
HhaI 501 GCGC GCG^C
HinP1I 501 GCGC G^CGC
HincII 246 GTTGAC GT[CT]^[GA]AC
HindII 246 GTTGAC GT[CT]^[GA]AC
HpaII 712 CCGG C^CGG
KpnI 3166 GGTACC GGTAC^C
MaeII 306 ACGT A^CGT
MamI 1018 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 145 CAATTG C^AATTG
MluNI 2436 TGGCCA TGG^CCA
MscI 2436 TGGCCA TGG^CCA
MspI 712 CCGG C^CGG
MunI 145 CAATTG C^AATTG
MvaI 736 CCAGG CC^[AT]GG
NsiI 2760 ATGCAT ATGCA^T
NspI 2033 ACATGC [GA]CATG^[CT]
PaeR7I 1421 CTCGAG C^TCGAG
PflMI 3223 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 171 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3243 ATTAAT AT^TAAT
SacI 721 GAGCTC GAGCT^C
ScaI 1193 AGTACT AGT^ACT
ScrFI 736 CCAGG CC^[ACGT]GG
SpeI 2776 ACTAGT A^CTAGT
SspBI 3183 TGTACA T^GTACA
Van91I 3223 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1421 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AccI 264 GTATAC GT^[AC][GT]AC
694 GTAGAC
AciI 7 GCGG CCGC(-3/-1)
3472 GCGG
AocI 907 CCTGAGG CC^T[ACGT]AGG
1339 CCTGAGG
BpmI 398 CTCCAG CTGGAG(16/14)
946 CTCCAG
BsmBI 505 CGTCTC CGTCTC(1/5)
303 GAGACG
Bsu36I 907 CCTGAGG CC^T[ACGT]AGG
1339 CCTGAGG
Cac8I 18 GCCTGC GC[ACGT]^[ACGT]GC
751 GCAAGC
Eco81I 907 CCTGAGG CC^T[ACGT]AGG
1339 CCTGAGG
EcoT14I 1113 CCTTGG C^C[AT][AT]GG
2867 CCTTGG
PstI 1526 CTGCAG CTGCA^G
3287 CTGCAG
StyI 1113 CCTTGG C^C[AT][AT]GG
2867 CCTTGG
Tsp45I 1067 GTGAC ^GT[GC]AC
1676 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanI BbsI BbvI BfaI
BglII BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsrI
BstF5I BstYI Cfr13I DdeI DpnII
EarI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII