(Circular) (Six-base) MAPSORT of: pFN21AE3393.seq Check: 2710 from: 1 to: 8269 pFN21AE3393 8269 bp With 182 enzymes: SgfI * September 21, 2017 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4990 4990 Size: 8269 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6079 278 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2270 2700 5612 1937 Size: 333 430 2912 4594 Fragments arranged by size: 4594 2912 430 333 Acc65I G'GTAC_C Cuts at: 5171 5590 5171 Size: 419 7850 AclI AA'CG_TT Cuts at: 1563 6396 6769 1563 Size: 4833 373 3063 Fragments arranged by size: 4833 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 2732 2877 2952 3028 3708 4298 4827 Size: 846 254 145 75 76 680 590 529 Cuts at: 4827 5124 5433 6332 7716 7984 1632 Size: 297 309 899 1384 268 1917 Fragments arranged by size: 1917 1384 899 846 680 590 529 309 297 268 254 145 76 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8072 AflIII A'CryG_T Cuts at: 1203 7168 8145 1203 Size: 5965 977 1327 Fragments arranged by size: 5965 1327 977 AgeI A'CCGG_T Cuts at: 7100 7100 Size: 8269 AhdI GACnn_n'nnGTC Cuts at: 6998 6998 Size: 8269 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8269 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8269 AlwNI CAG_nnn'CTG Cuts at: 5510 6012 7584 5510 Size: 502 1572 6195 Fragments arranged by size: 6195 1572 502 ApaLI G'TGCA_C Cuts at: 6326 7482 6326 Size: 1156 7113 ApoI r'AATT_y Cuts at: 1493 2663 3105 3136 3436 3774 4129 4180 4699 Size: 1170 442 31 300 338 355 51 519 Cuts at: 4699 5578 5804 5956 8065 1493 Size: 879 226 152 2109 1697 Fragments arranged by size: 2109 1697 1170 879 519 442 355 338 300 226 152 51 31 AseI AT'TA_AT Cuts at: 160 1032 5249 6823 160 Size: 872 4217 1574 1606 Fragments arranged by size: 4217 1606 1574 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 AvaI C'yCGr_G Cuts at: 1870 1943 3426 5594 1870 Size: 73 1483 2168 4545 Fragments arranged by size: 4545 2168 1483 73 BamHI G'GATC_C Cuts at: 5599 5966 5599 Size: 367 7902 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2908 5171 5590 7045 Size: 325 217 627 282 839 2263 419 1455 Cuts at: 7045 618 Size: 1842 Fragments arranged by size: 2263 1842 1455 839 627 419 325 282 217 BanII G_rGCy'C Cuts at: 1391 2730 5588 1391 Size: 1339 2858 4072 Fragments arranged by size: 4072 2858 1339 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2426 2505 2982 3480 3714 3770 4393 Size: 971 527 79 477 498 234 56 623 Cuts at: 4393 4488 928 Size: 95 4709 Fragments arranged by size: 4709 971 623 527 498 477 234 95 79 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2036 2070 6460 6494 1903 Size: 34 99 34 4390 34 3678 Fragments arranged by size: 4390 3678 99 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2586 6161 7371 2586 Size: 3575 1210 3484 Fragments arranged by size: 3575 3484 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8269 BfrBI ATG'CAT Cuts at: 4767 4767 Size: 8269 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6880 136 Size: 107 122 71 631 605 337 4871 1525 Fragments arranged by size: 4871 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2438 3022 4405 8264 2438 Size: 584 1383 3859 2443 Fragments arranged by size: 3859 2443 1383 584 BlpI GC'TnA_GC Cuts at: 5686 5686 Size: 8269 Bme1580I G_kGCm'C Cuts at: 1790 6330 7486 1790 Size: 4540 1156 2573 Fragments arranged by size: 4540 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8269 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3569 6000 6958 Size: 416 53 83 399 24 2152 2431 958 Cuts at: 6958 442 Size: 1753 Fragments arranged by size: 2431 2152 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8269 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2460 3441 4978 5371 2460 Size: 981 1537 393 5358 Fragments arranged by size: 5358 1537 981 393 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 2936 6929 1239 Size: 1149 548 3993 2579 Fragments arranged by size: 3993 2579 1149 548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2840 4635 5097 6394 7259 7557 2840 Size: 1795 462 1297 865 298 3552 Fragments arranged by size: 3552 1795 1297 865 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5126 6932 882 Size: 617 3627 1806 2219 Fragments arranged by size: 3627 2219 1806 617 BsaAI yAC'GTr Cuts at: 493 1204 6060 493 Size: 711 4856 2702 Fragments arranged by size: 4856 2702 711 BsaBI GATnn'nnATC Cuts at: 3027 8263 3027 Size: 5236 3033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6076 6458 7093 275 Size: 53 83 186 936 4543 382 635 1451 Fragments arranged by size: 4543 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2716 6702 7100 7374 7521 7932 2716 Size: 3986 398 274 147 411 3053 Fragments arranged by size: 3986 3053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 1178 Size: 30 165 30 781 30 7233 Fragments arranged by size: 7233 781 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3209 3995 5449 1186 Size: 2023 786 1454 4006 Fragments arranged by size: 4006 2023 1454 786 BseYI C'CCAG_C Cuts at: 2597 2851 7472 7968 2597 Size: 254 4621 496 2898 Fragments arranged by size: 4621 2898 496 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4209 1163 Size: 573 2473 5223 Fragments arranged by size: 5223 2473 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5719 6480 7508 1540 Size: 275 186 3718 761 1028 2301 Fragments arranged by size: 3718 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 6330 6415 7486 2730 Size: 3600 85 1071 3513 Fragments arranged by size: 3600 3513 1071 85 BsmI GAATG_Cn' Cuts at: 3123 4740 5567 5780 5873 3123 Size: 1617 827 213 93 5519 Fragments arranged by size: 5519 1617 827 213 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2302 2516 2302 Size: 214 8055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2730 5588 6330 6415 7486 1391 Size: 399 940 2858 742 85 1071 2174 Fragments arranged by size: 2858 2174 1071 940 742 399 85 BspEI T'CCGG_A Cuts at: 7932 7932 Size: 8269 BspHI T'CATG_A Cuts at: 6158 7888 6158 Size: 1730 6539 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4990 5624 844 Size: 4146 634 3489 Fragments arranged by size: 4146 3489 634 BsrBI CCG'CTC Cuts at: 1394 1823 6156 1394 Size: 429 4333 3507 Fragments arranged by size: 4333 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4854 6764 6938 65 Size: 1183 3606 1910 174 1396 Fragments arranged by size: 3606 1910 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6913 7100 1700 Size: 110 5103 187 2869 Fragments arranged by size: 5103 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5188 96 Size: 5092 3177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8269 BssSI C'ACGA_G Cuts at: 6329 7341 6329 Size: 1012 7257 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8269 BstBI TT'CG_AA Cuts at: 7073 7073 Size: 8269 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8269 BstXI CCAn_nnnn'nTGG Cuts at: 2152 2152 Size: 8269 BstYI r'GATC_y Cuts at: 1972 2222 2438 3022 4405 4676 5599 5966 6351 Size: 250 216 584 1383 271 923 367 385 Cuts at: 6351 6368 7820 7906 7918 8264 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1383 923 584 385 367 346 271 250 216 86 17 12 BstZ17I GTA'TAC Cuts at: 2271 2271 Size: 8269 Bsu36I CC'TnA_GG Cuts at: 2913 3345 2913 Size: 432 7837 BtgI C'CryG_G Cuts at: 513 1065 2274 513 Size: 552 1209 6508 Fragments arranged by size: 6508 1209 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4578 487 Size: 683 56 3352 4178 Fragments arranged by size: 4178 3352 683 56 BtsI GCAGTG_nn' Cuts at: 2488 5785 6579 6599 2488 Size: 3297 794 20 4158 Fragments arranged by size: 4158 3297 794 20 ClaI AT'CG_AT Cuts at: 1554 3357 1554 Size: 1803 6466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2991 3026 529 Size: 35 2427 35 5772 Fragments arranged by size: 5772 2427 35 35 DraI TTT'AAA Cuts at: 4017 5573 4017 Size: 1556 6713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7276 809 Size: 507 5960 1802 Fragments arranged by size: 5960 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4441 5716 6605 Size: 54 1425 50 275 18 2611 1275 889 Cuts at: 6605 8234 8 Size: 1629 43 Fragments arranged by size: 2611 1629 1425 1275 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5716 1537 Size: 275 3904 4090 Fragments arranged by size: 4090 3904 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2451 2481 2865 2898 2928 3038 3047 3285 Size: 1098 30 384 33 30 110 9 238 Cuts at: 3285 3329 3378 3456 3630 3636 5216 6199 1353 Size: 44 49 78 174 6 1580 983 3423 Fragments arranged by size: 3423 1580 1098 983 384 238 174 110 78 49 44 33 30 30 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6856 7230 7376 387 Size: 1064 138 5267 374 146 1280 Fragments arranged by size: 5267 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2728 2728 Size: 8269 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2478 2732 2877 2936 2952 3028 Size: 393 756 90 254 145 59 16 76 Cuts at: 3028 3708 4298 4827 5124 5433 6332 6929 7716 Size: 680 590 529 297 309 899 597 787 Cuts at: 7716 7984 1239 Size: 268 1524 Fragments arranged by size: 1524 899 787 756 680 597 590 529 393 309 297 268 254 145 90 76 59 16 EcoNI CCTnn'n_nnAGG Cuts at: 2132 2132 Size: 8269 EcoO109I rG'GnC_Cy Cuts at: 2177 2177 Size: 8269 EcoRI G'AATT_C Cuts at: 3136 5578 5956 3136 Size: 2442 378 5449 Fragments arranged by size: 5449 2442 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2457 2457 Size: 8269 FspI TGC'GCA Cuts at: 6775 6775 Size: 8269 HaeII r_GCGC'y Cuts at: 1678 2509 7416 1678 Size: 831 4907 2531 Fragments arranged by size: 4907 2531 831 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3611 3643 3837 3869 4390 Size: 32 135 32 2039 32 194 32 521 Cuts at: 4390 4422 5120 5152 6910 6942 6984 7016 1373 Size: 32 698 32 1758 32 42 32 2626 Fragments arranged by size: 2626 2039 1758 698 521 194 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2253 5613 5859 669 Size: 988 281 315 3360 246 3079 Fragments arranged by size: 3360 3079 988 315 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8269 HpaI GTT'AAC Cuts at: 5859 5859 Size: 8269 KpnI G_GTAC'C Cuts at: 5175 5594 5175 Size: 419 7850 MfeI C'AATT_G Cuts at: 2150 5868 2150 Size: 3718 4551 MluI A'CGCG_T Cuts at: 8145 8145 Size: 8269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2170 4270 4795 5026 7383 7567 1305 Size: 865 2100 525 231 2357 184 2007 Fragments arranged by size: 2357 2100 2007 865 525 231 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4443 10 Size: 54 1425 343 2611 3836 Fragments arranged by size: 3836 2611 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 3063 3240 3840 5513 6227 6586 Size: 648 1452 445 177 600 1673 714 359 Cuts at: 6586 6745 518 Size: 159 2042 Fragments arranged by size: 2042 1673 1452 714 648 600 445 359 177 159 MspA1I CmG'CkG Cuts at: 1606 2601 2855 3143 4805 5685 6362 7510 7755 Size: 995 254 288 1662 880 677 1148 245 Cuts at: 7755 1606 Size: 2120 Fragments arranged by size: 2120 1662 1148 995 880 677 288 254 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7717 NdeI CA'TA_TG Cuts at: 387 387 Size: 8269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8269 NotI GC'GGCC_GC Cuts at: 5716 5716 Size: 8269 NsiI A_TGCA'T Cuts at: 4769 4769 Size: 8269 NspI r_CATG'y Cuts at: 1132 1229 4042 5627 7172 1132 Size: 97 2813 1585 1545 2229 Fragments arranged by size: 2813 2229 1585 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8269 PciI A'CATG_T Cuts at: 7168 7168 Size: 8269 PflMI CCAn_nnn'nTGG Cuts at: 1108 5234 1108 Size: 4126 4143 PmeI GTTT'AAAC Cuts at: 5573 5573 Size: 8269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6338 6370 7851 7883 6338 Size: 32 1481 32 6724 Fragments arranged by size: 6724 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2177 2177 Size: 8269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8269 PsiI TTA'TAA Cuts at: 2964 4424 5839 2964 Size: 1460 1415 5394 Fragments arranged by size: 5394 1460 1415 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4293 4325 4293 Size: 32 8237 PstI C_TGCA'G Cuts at: 830 3535 5296 5621 830 Size: 2705 1761 325 3478 Fragments arranged by size: 3478 2705 1761 325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8269 PvuII CAG'CTG Cuts at: 2601 2855 3143 4805 2601 Size: 254 288 1662 6065 Fragments arranged by size: 6065 1662 288 254 SacI G_AGCT'C Cuts at: 2730 2730 Size: 8269 SalI G'TCGA_C Cuts at: 1936 5611 1936 Size: 3675 4594 SbfI CC_TGCA'GG Cuts at: 5621 5621 Size: 8269 ScaI AGT'ACT Cuts at: 3200 6517 8229 3200 Size: 3317 1712 3240 Fragments arranged by size: 3317 3240 1712 SfcI C'TryA_G Cuts at: 826 1045 2673 3531 4527 4995 5176 5292 5375 Size: 219 1628 858 996 468 181 116 83 Cuts at: 5375 5556 5617 6752 7433 7624 826 Size: 181 61 1135 681 191 1471 Fragments arranged by size: 1628 1471 1135 996 858 681 468 219 191 181 181 116 83 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8269 SmaI CCC'GGG Cuts at: 1872 5596 1872 Size: 3724 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 3426 4650 5112 6373 7274 Size: 197 926 876 607 1224 462 1261 901 Cuts at: 7274 7536 820 Size: 262 1553 Fragments arranged by size: 1553 1261 1224 926 901 876 607 462 262 197 SnaBI TAC'GTA Cuts at: 493 6060 493 Size: 5567 2702 SpeI A'CTAG_T Cuts at: 152 4781 152 Size: 4629 3640 SphI G_CATG'C Cuts at: 1132 5627 1132 Size: 4495 3774 SspI AAT'ATT Cuts at: 5 52 3079 3217 4822 5393 6193 5 Size: 47 3027 138 1605 571 800 2081 Fragments arranged by size: 3027 2081 1605 800 571 138 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8269 StyI C'CwwG_G Cuts at: 513 1065 3118 4872 5708 513 Size: 552 2053 1754 836 3074 Fragments arranged by size: 3074 2053 1754 836 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3859 6296 6313 6466 6651 7096 325 Size: 1255 2279 2437 17 153 185 445 1498 Fragments arranged by size: 2437 2279 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3198 4309 5188 Size: 274 80 33 51 1443 1221 1111 879 Cuts at: 5188 5342 5548 6515 8227 96 Size: 154 206 967 1712 138 Fragments arranged by size: 1712 1443 1221 1111 967 879 274 206 154 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3723 3755 8229 8261 3723 Size: 32 4474 32 3731 Fragments arranged by size: 4474 3731 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8269 XbaI T'CTAG_A Cuts at: 5605 5605 Size: 8269 XhoI C'TCGA_G Cuts at: 1943 3426 1943 Size: 1483 6786 XmaI C'CCGG_G Cuts at: 1870 5594 1870 Size: 3724 4545 XmnI GAAnn'nnTTC Cuts at: 6398 6398 Size: 8269 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6077 276 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI