################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbsI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspMI BsrDI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MseI MslI MunI MvnI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SfcI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 154 GACGTC GACGT^C AflIII 635 ACATGT A^C[GA][CT]GT AgeI 160 ACCGGT A^CCGGT Alw44I 439 GTGCAC G^TGCAC AlwNI 703 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 439 GTGCAC G^TGCAC AviII 233 TGCGCA TGC^GCA BalI 1065 TGGCCA TGG^CCA BbvCI 714 GCTGAGG CCTCAGC(-5/-2) BcgI 544 CGAGAGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 722 TGATCA T^GATCA BglI 684 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 993 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 659 TGTACA T^GTACA BspHI 725 TCATGA T^CATGA BspLU11I 635 ACATGT A^CATGT BsrBI 729 GAGCGG CCGCTC(-3/-3) BsrGI 659 TGTACA T^GTACA BssSI 123 CACGAG CACGAG(-5/-1) BstEII 357 GGTCACC G^GT[ACGT]ACC BstPI 357 GGTCACC G^GT[ACGT]ACC BstXI 563 CCAGTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1069 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 357 GGTCACC G^GT[ACGT]ACC EcoT22I 108 ATGCAT ATGCA^T FbaI 722 TGATCA T^GATCA FspI 233 TGCGCA TGC^GCA MaeII 155 ACGT A^CGT MamI 993 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1065 TGGCCA TGG^CCA MscI 1065 TGGCCA TGG^CCA NsiI 108 ATGCAT ATGCA^T NspI 635 ACATGT [GA]CATG^[CT] PinAI 160 ACCGGT A^CCGGT PleI 25 GACTC GAGTC(4/5) RcaI 725 TCATGA T^CATGA ScaI 869 AGTACT AGT^ACT SexAI 788 ACCTGGT A^CC[AT]GGT SgrAI 159 CACCGGTG C[GA]^CCGG[CT]G SmlI 363 CTTGAG C^T[CT][GA]AG SspBI 659 TGTACA T^GTACA StuI 842 AGGCCT AGG^CCT Tsp509I 55 AATT ^AATT XcmI 65 CCAAAGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 72 GATCC GGATC(4/5) 950 GGATC BanI 248 GGCACC G^G[CT][GA]CC 692 GGCACC BpmI 403 CTGGAG CTGGAG(16/14) 523 CTGGAG BsaWI 160 ACCGGT [AT]^CCGG[AT] 695 ACCGGA BsgI 35 CTGCAC GTGCAG(16/14) 414 CTGCAC BstYI 71 AGATCC [GA]^GATC[CT] 950 GGATCT CfoI 234 GCGC GCG^C 1029 GCGC EarI 321 GAAGAG CTCTTC(1/4) 838 GAAGAG EcoNI 231 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1143 CCTGGCCGAGG EcoT14I 845 CCTTGG C^C[AT][AT]GG 1124 CCAAGG HgaI 1042 GACGC GACGC(5/10) 650 GCGTC HhaI 234 GCGC GCG^C 1029 GCGC HinP1I 234 GCGC G^CGC 1029 GCGC Ksp632I 321 GAAGAG CTCTTC(1/4) 838 GAAGAG MflI 71 AGATCC [GA]^GATC[CT] 950 GGATCT NaeI 673 GCCGGC GCC^GGC 1031 GCCGGC NgoMIV 673 GCCGGC G^CCGGC 1031 GCCGGC PvuII 265 CAGCTG CAG^CTG 520 CAGCTG SacI 331 GAGCTC GAGCT^C 395 GAGCTC StyI 845 CCTTGG C^C[AT][AT]GG 1124 CCAAGG TfiI 800 GATTC G^A[AT]TC 1058 GAATC XhoII 71 AGATCC [GA]^GATC[CT] 950 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcyI AfaI AluI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI