(Linear) (Six-base) MAPSORT of: KIEE0662.seq Check: 9726 from: 1 to: 1161 KIEE0662.orf With 182 enzymes: SgfI * July 3, 2012 16:40 .. AatII G_ACGT'C Cuts at: 0 158 1161 Size: 158 1003 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 235 289 472 879 926 1161 Size: 159 76 54 183 407 47 235 Fragments arranged by size: 407 235 183 159 76 54 47 AflIII A'CryG_T Cuts at: 0 635 1161 Size: 635 526 AgeI A'CCGG_T Cuts at: 0 160 1161 Size: 160 1001 AlwNI CAG_nnn'CTG Cuts at: 0 708 1161 Size: 708 453 ApaLI G'TGCA_C Cuts at: 0 439 1161 Size: 439 722 AvaI C'yCGr_G Cuts at: 0 5 457 612 754 902 986 1161 Size: 5 452 155 142 148 84 175 Fragments arranged by size: 452 175 155 148 142 84 5 BanI G'GyrC_C Cuts at: 0 248 692 1161 Size: 248 444 469 Fragments arranged by size: 469 444 248 BanII G_rGCy'C Cuts at: 0 65 335 399 1161 Size: 65 270 64 762 Fragments arranged by size: 762 270 65 64 BbvCI CC'TCA_GC Cuts at: 0 715 1161 Size: 715 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 533 567 1161 Size: 533 34 594 Fragments arranged by size: 594 533 34 BclI T'GATC_A Cuts at: 0 722 1161 Size: 722 439 BfrBI ATG'CAT Cuts at: 0 110 1161 Size: 110 1051 BglI GCCn_nnn'nGGC Cuts at: 0 690 1161 Size: 690 471 Bme1580I G_kGCm'C Cuts at: 0 251 443 1161 Size: 251 192 718 Fragments arranged by size: 718 251 192 BmrI ACTGGGnnnn_n' Cuts at: 0 557 1080 1161 Size: 557 523 81 Fragments arranged by size: 557 523 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 349 1161 Size: 349 812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 544 1161 Size: 424 120 617 Fragments arranged by size: 617 424 120 Bpu10I CC'TnA_GC Cuts at: 0 715 1161 Size: 715 446 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 1161 Size: 384 777 BsaI GGTCTCn'nnnn_ Cuts at: 0 246 277 998 1161 Size: 246 31 721 163 Fragments arranged by size: 721 246 163 31 BsaBI GATnn'nnATC Cuts at: 0 997 1161 Size: 997 164 BsaHI Gr'CG_yC Cuts at: 0 155 650 1043 1161 Size: 155 495 393 118 Fragments arranged by size: 495 393 155 118 BsaWI w'CCGG_w Cuts at: 0 160 695 1161 Size: 160 535 466 Fragments arranged by size: 535 466 160 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 199 1126 1161 Size: 199 927 35 Fragments arranged by size: 927 199 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 399 1161 Size: 20 379 762 Fragments arranged by size: 762 379 20 BsiHKAI G_wGCw'C Cuts at: 0 335 399 443 595 646 1136 1161 Size: 335 64 44 152 51 490 25 Fragments arranged by size: 490 335 152 64 51 44 25 Bsp1286I G_dGCh'C Cuts at: 0 65 251 335 399 443 595 646 1136 Size: 65 186 84 64 44 152 51 490 Cuts at: 1136 1161 Size: 25 Fragments arranged by size: 490 186 152 84 65 64 51 44 25 BspHI T'CATG_A Cuts at: 0 725 1161 Size: 725 436 BsrBI CCG'CTC Cuts at: 0 731 1161 Size: 731 430 BsrFI r'CCGG_y Cuts at: 0 160 673 1031 1161 Size: 160 513 358 130 Fragments arranged by size: 513 358 160 130 BsrGI T'GTAC_A Cuts at: 0 659 1161 Size: 659 502 BssSI C'ACGA_G Cuts at: 0 123 1161 Size: 123 1038 BstEII G'GTnAC_C Cuts at: 0 357 1161 Size: 357 804 BstXI CCAn_nnnn'nTGG Cuts at: 0 570 1161 Size: 570 591 BstYI r'GATC_y Cuts at: 0 71 950 1161 Size: 71 879 211 Fragments arranged by size: 879 211 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 120 1161 Size: 120 1041 BtsI GCAGTG_nn' Cuts at: 0 16 99 1161 Size: 16 83 1062 Fragments arranged by size: 1062 83 16 DraIII CAC_nnn'GTG Cuts at: 0 1074 1161 Size: 1074 87 EaeI y'GGCC_r Cuts at: 0 671 1065 1145 1161 Size: 671 394 80 16 Fragments arranged by size: 671 394 80 16 EarI CTCTTCn'nnn_ Cuts at: 0 316 833 1161 Size: 316 517 328 Fragments arranged by size: 517 328 316 EcoICRI GAG'CTC Cuts at: 0 333 397 1161 Size: 333 64 764 Fragments arranged by size: 764 333 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 235 289 424 472 544 879 926 Size: 159 76 54 135 48 72 335 47 Cuts at: 926 1161 Size: 235 Fragments arranged by size: 335 235 159 135 76 72 54 48 47 EcoNI CCTnn'n_nnAGG Cuts at: 0 235 1147 1161 Size: 235 912 14 Fragments arranged by size: 912 235 14 EcoO109I rG'GnC_Cy Cuts at: 0 447 557 786 1161 Size: 447 110 229 375 Fragments arranged by size: 447 375 229 110 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 378 1161 Size: 378 783 FspI TGC'GCA Cuts at: 0 235 1161 Size: 235 926 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 754 786 1161 Size: 754 32 375 Fragments arranged by size: 754 375 32 MscI TGG'CCA Cuts at: 0 1067 1161 Size: 1067 94 MspA1I CmG'CkG Cuts at: 0 267 522 835 1161 Size: 267 255 313 326 Fragments arranged by size: 326 313 267 255 NaeI GCC'GGC Cuts at: 0 675 1033 1161 Size: 675 358 128 Fragments arranged by size: 675 358 128 NgoMIV G'CCGG_C Cuts at: 0 673 1031 1161 Size: 673 358 130 Fragments arranged by size: 673 358 130 NsiI A_TGCA'T Cuts at: 0 112 1161 Size: 112 1049 NspI r_CATG'y Cuts at: 0 639 1161 Size: 639 522 PciI A'CATG_T Cuts at: 0 635 1161 Size: 635 526 PpuMI rG'GwC_Cy Cuts at: 0 447 557 786 1161 Size: 447 110 229 375 Fragments arranged by size: 447 375 229 110 PvuII CAG'CTG Cuts at: 0 267 522 1161 Size: 267 255 639 Fragments arranged by size: 639 267 255 SacI G_AGCT'C Cuts at: 0 335 399 1161 Size: 335 64 762 Fragments arranged by size: 762 335 64 SanDI GG'GwC_CC Cuts at: 0 557 1161 Size: 557 604 ScaI AGT'ACT Cuts at: 0 871 1161 Size: 871 290 SexAI A'CCwGG_T Cuts at: 0 788 1161 Size: 788 373 SgrAI Cr'CCGG_yG Cuts at: 0 160 1161 Size: 160 1001 SmaI CCC'GGG Cuts at: 0 7 614 904 988 1161 Size: 7 607 290 84 173 Fragments arranged by size: 607 290 173 84 7 SmlI C'TyrA_G Cuts at: 0 363 1161 Size: 363 798 StuI AGG'CCT Cuts at: 0 844 1161 Size: 844 317 StyI C'CwwG_G Cuts at: 0 845 1124 1161 Size: 845 279 37 Fragments arranged by size: 845 279 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1062 1137 1161 Size: 1062 75 24 Fragments arranged by size: 1062 75 24 TatI w'GTAC_w Cuts at: 0 659 869 1161 Size: 659 210 292 Fragments arranged by size: 659 292 210 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 72 1161 Size: 72 1089 XmaI C'CCGG_G Cuts at: 0 5 612 902 986 1161 Size: 5 607 290 84 175 Fragments arranged by size: 607 290 175 84 5 ZraI GAC'GTC Cuts at: 0 156 1161 Size: 156 1005 Enzymes that do cut: AatII AcuI AflIII AgeI AlwNI ApaLI AvaI BanI BanII BbvCI BcgI BclI BfrBI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI Hin4I MscI MspA1I NaeI NgoMIV NsiI NspI PciI PpuMI PvuII SacI SanDI ScaI SexAI SgrAI SmaI SmlI StuI StyI TaqII TatI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BglII BlpI BmgBI BmtI BsaAI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I BtgI ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NarI NcoI NdeI NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI SfcI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmnI