################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BbvCI BciVI BcnI BfrI BglI BglII BlnI BpuAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI SspI StyI SwaI TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 94 AAATTC [GA]^AATT[CT] AfaI 372 GTAC GT^AC ApoI 94 AAATTC [GA]^AATT[CT] AvaII 543 GGACC G^G[AT]CC BalI 500 TGGCCA TGG^CCA BanII 853 GAGCTC G[GA]GC[CT]^C BbrPI 260 CACGTG CAC^GTG BcgI 804 CGACCTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 530 TGATCA T^GATCA BpmI 654 CTGGAG CTGGAG(16/14) BsaAI 260 CACGTG [CT]AC^GT[GA] BsaI 433 GAGACC GGTCTC(1/5) BsaWI 227 ACCGGA [AT]^CCGG[AT] BseRI 159 GAGGAG GAGGAG(10/8) BsmFI 594 GTCCC GGGAC(10/14) BsrBI 136 GAGCGG CCGCTC(-3/-3) BsrDI 255 CATTGC GCAATG(2/0) BsrI 450 CCAGT ACTGG(1/-1) BssSI 430 CACGAG CACGAG(-5/-1) Cfr13I 543 GGACC G^G[ACGT]CC EaeI 500 TGGCCA [CT]^GGCC[GA] EcoNI 71 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 530 TGATCA T^GATCA HapII 228 CCGG C^CGG HpaII 228 CCGG C^CGG HphI 533 TCACC GGTGA(8/7) MluNI 500 TGGCCA TGG^CCA MscI 500 TGGCCA TGG^CCA MseI 883 TTAA T^TAA MspI 228 CCGG C^CGG NlaIV 543 GGACCC GG[ACGT]^[ACGT]CC PleI 243 GACTC GAGTC(4/5) PmaCI 260 CACGTG CAC^GTG PmlI 260 CACGTG CAC^GTG RsaI 372 GTAC GT^AC SacI 853 GAGCTC GAGCT^C SapI 402 GCTCTTC GCTCTTC(1/4) Sau96I 543 GGACC G^G[ACGT]CC SbfI 689 CCTGCAGG CCTGCA^GG SphI 722 GCATGC GCATG^C Sse8387I 689 CCTGCAGG CCTGCA^GG StuI 176 AGGCCT AGG^CCT TfiI 201 GATTC G^A[AT]TC Tru9I 883 TTAA T^TAA Tsp45I 263 GTGAC ^GT[GC]AC Tsp509I 95 AATT ^AATT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 289 GATCC GGATC(4/5) 333 GATCC BbvI 726 GCTGC GCAGC(8/12) 750 GCTGC BfaI 325 CTAG C^TAG 511 CTAG BsmAI 241 GAGAC GTCTC(1/5) 433 GAGAC BspMI 221 ACCTGC ACCTGC(4/8) 744 GCAGGT BstAPI 729 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 744 GCAGGTGCTGC BstYI 288 AGATCC [GA]^GATC[CT] 332 AGATCC FauI 738 GCGGG CCCGC(4/6) 767 GCGGG HinfI 201 GATTC G^A[ACGT]TC 243 GACTC MaeI 325 CTAG C^TAG 511 CTAG MaeIII 263 GTGAC ^GT[ACGT]AC 347 GTTAC MflI 288 AGATCC [GA]^GATC[CT] 332 AGATCC MslI 475 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 492 CATTGGCGTG MspA1I 409 CAGCTG C[AC]G^C[GT]G 477 CAGCTG NspI 515 ACATGC [GA]CATG^[CT] 722 GCATGC PflMI 408 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 702 CCAGGTGCTGG PvuII 409 CAGCTG CAG^CTG 477 CAGCTG SfaNI 399 GATGC GCATC(5/9) 811 GATGC TseI 726 GCTGC G^C[AT]GC 750 GCTGC Van91I 408 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 702 CCAGGTGCTGG XhoII 288 AGATCC [GA]^GATC[CT] 332 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AlwNI AspHI BlpI BmyI Bpu1102I BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CelII DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MaeII MboI MboII MnlI MvaI MwoI NdeII NlaIII PstI Sau3AI ScrFI SfcI TaqI TthHB8I