(Linear) (Six-base) MAPSORT of: KIEE4062.seq Check: 7625 from: 1 to: 900 KIEE4062.orf With 182 enzymes: SgfI * July 3, 2012 18:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 735 900 Size: 735 165 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 900 Size: 104 796 AleI CACnn'nnGTG Cuts at: 0 479 900 Size: 479 421 AlwNI CAG_nnn'CTG Cuts at: 0 586 708 750 900 Size: 586 122 42 150 Fragments arranged by size: 586 150 122 42 ApoI r'AATT_y Cuts at: 0 94 900 Size: 94 806 BanII G_rGCy'C Cuts at: 0 857 900 Size: 857 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 793 827 900 Size: 793 34 73 Fragments arranged by size: 793 73 34 BclI T'GATC_A Cuts at: 0 530 900 Size: 530 370 BlpI GC'TnA_GC Cuts at: 0 520 557 851 900 Size: 520 37 294 49 Fragments arranged by size: 520 294 49 37 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 843 900 Size: 843 57 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 675 900 Size: 675 225 BsaI GGTCTCn'nnnn_ Cuts at: 0 427 900 Size: 427 473 BsaAI yAC'GTr Cuts at: 0 262 900 Size: 262 638 BsaWI w'CCGG_w Cuts at: 0 227 900 Size: 227 673 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 27 900 Size: 27 873 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 174 900 Size: 174 726 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 208 599 610 900 Size: 49 159 391 11 290 Fragments arranged by size: 391 290 159 49 11 BsiHKAI G_wGCw'C Cuts at: 0 425 563 857 900 Size: 425 138 294 43 Fragments arranged by size: 425 294 138 43 Bsp1286I G_dGCh'C Cuts at: 0 425 563 857 900 Size: 425 138 294 43 Fragments arranged by size: 425 294 138 43 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 230 735 900 Size: 230 505 165 Fragments arranged by size: 505 230 165 BsrBI CCG'CTC Cuts at: 0 138 900 Size: 138 762 BsrDI GCAATG_nn' Cuts at: 0 254 900 Size: 254 646 BssSI C'ACGA_G Cuts at: 0 430 900 Size: 430 470 BstAPI GCAn_nnn'nTGC Cuts at: 0 735 750 900 Size: 735 15 150 Fragments arranged by size: 735 150 15 BstYI r'GATC_y Cuts at: 0 288 332 900 Size: 288 44 568 Fragments arranged by size: 568 288 44 EaeI y'GGCC_r Cuts at: 0 500 900 Size: 500 400 EarI CTCTTCn'nnn_ Cuts at: 0 313 409 604 900 Size: 313 96 195 296 Fragments arranged by size: 313 296 195 96 EcoICRI GAG'CTC Cuts at: 0 855 900 Size: 855 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 675 900 Size: 104 571 225 Fragments arranged by size: 571 225 104 EcoNI CCTnn'n_nnAGG Cuts at: 0 75 900 Size: 75 825 MscI TGG'CCA Cuts at: 0 502 900 Size: 502 398 MslI CAynn'nnrTG Cuts at: 0 479 496 900 Size: 479 17 404 Fragments arranged by size: 479 404 17 MspA1I CmG'CkG Cuts at: 0 411 479 900 Size: 411 68 421 Fragments arranged by size: 421 411 68 NspI r_CATG'y Cuts at: 0 519 726 900 Size: 519 207 174 Fragments arranged by size: 519 207 174 PasI CC'CwG_GG Cuts at: 0 294 900 Size: 294 606 PflMI CCAn_nnn'nTGG Cuts at: 0 414 708 900 Size: 414 294 192 Fragments arranged by size: 414 294 192 PfoI T'CCnGG_A Cuts at: 0 539 900 Size: 539 361 PmlI CAC'GTG Cuts at: 0 262 900 Size: 262 638 PstI C_TGCA'G Cuts at: 0 464 694 731 755 900 Size: 464 230 37 24 145 Fragments arranged by size: 464 230 145 37 24 PvuII CAG'CTG Cuts at: 0 411 479 900 Size: 411 68 421 Fragments arranged by size: 421 411 68 SacI G_AGCT'C Cuts at: 0 857 900 Size: 857 43 SapI GCTCTTCn'nnn_ Cuts at: 0 409 900 Size: 409 491 SbfI CC_TGCA'GG Cuts at: 0 694 900 Size: 694 206 SfcI C'TryA_G Cuts at: 0 460 690 717 727 751 900 Size: 460 230 27 10 24 149 Fragments arranged by size: 460 230 149 27 24 10 SphI G_CATG'C Cuts at: 0 726 900 Size: 726 174 StuI AGG'CCT Cuts at: 0 178 900 Size: 178 722 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 29 900 Size: 29 871 Enzymes that do cut: AarI AcuI AleI AlwNI ApoI BanII BcgI BclI BlpI BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstYI EaeI EarI EcoICRI Eco57MI EcoNI MscI MslI MspA1I NspI PasI PflMI PfoI PmlI PstI PvuII SacI SapI SbfI SfcI SphI StuI TstI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaHI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI