################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I ClaI DraI DraIII Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MaeI MfeI MflI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 6 GACGCC G[GA]^CG[CT]C AfaI 429 GTAC GT^AC AlwI 360 GATCC GGATC(4/5) AlwNI 139 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 757 CCTGAGG CC^T[ACGT]AGG ApaI 256 GGGCCC GGGCC^C Asp700I 159 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 840 GGCACC G^G[CT][GA]CC BglI 34 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 360 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 6 GACGCC G[GA]^CG[CT]C BsaWI 377 ACCGGA [AT]^CCGG[AT] BsgI 703 GTGCAG GTGCAG(16/14) BspMI 705 GCAGGT ACCTGC(4/8) BstEII 402 GGTCACC G^GT[ACGT]ACC BstPI 402 GGTCACC G^GT[ACGT]ACC BstXI 870 CCAGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 757 CCTGAGG CC^T[ACGT]AGG CpoI 203 CGGACCG CG^G[AT]CCG DrdI 394 GACTACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 10 CCGCGG C^C[GA][CT]GG Eco81I 757 CCTGAGG CC^T[ACGT]AGG EcoNI 177 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 402 GGTCACC G^GT[ACGT]ACC EcoT14I 575 CCAAGG C^C[AT][AT]GG Hin1I 6 GACGCC G[GA]^CG[CT]C HinfI 613 GAGTC G^A[ACGT]TC KspI 10 CCGCGG CCGC^GG MaeII 107 ACGT A^CGT MamI 360 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 65 GCGGCCGC GC^GGCCGC NspI 423 GCATGT [GA]CATG^[CT] PleI 613 GAGTC GAGTC(4/5) RsaI 429 GTAC GT^AC RsrII 203 CGGACCG CG^G[AT]CCG SacII 10 CCGCGG CCGC^GG SapI 190 GAAGAGC GCTCTTC(1/4) SgrAI 50 CGCCGGCG C[GA]^CCGG[CT]G StyI 575 CCAAGG C^C[AT][AT]GG TaqI 500 TCGA T^CGA TthHB8I 500 TCGA T^CGA XmnI 159 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### BbvCI 168 CCTCAGC CCTCAGC(-5/-2) 310 GCTGAGG BlpI 603 GCTGAGC GC^T[ACGT]AGC 763 GCTGAGC Bpu1102I 603 GCTGAGC GC^T[ACGT]AGC 763 GCTGAGC BsaI 214 GAGACC GGTCTC(1/5) 471 GGTCTC BsiEI 13 CGGCCG CG[GA][CT]^CG 66 CGGCCG BsmAI 214 GAGAC GTCTC(1/5) 472 GTCTC BsmFI 873 GGGAC GGGAC(10/14) 412 GTCCC BsrFI 15 GCCGGC [GA]^CCGG[CT] 51 GCCGGC CelII 603 GCTGAGC GC^T[ACGT]AGC 763 GCTGAGC Cfr10I 15 GCCGGC [GA]^CCGG[CT] 51 GCCGGC DpnII 360 GATC ^GATC 862 GATC EaeI 13 CGGCCG [CT]^GGCC[GA] 66 CGGCCG EagI 13 CGGCCG C^GGCCG 66 CGGCCG EarI 190 GAAGAG CTCTTC(1/4) 385 GAAGAG EclXI 13 CGGCCG C^GGCCG 66 CGGCCG Eco52I 13 CGGCCG C^GGCCG 66 CGGCCG HaeII 45 AGCGCC [GA]GCGC^[CT] 494 GGCGCT HgaI 6 GACGC GACGC(5/10) 141 GACGC Ksp632I 190 GAAGAG CTCTTC(1/4) 385 GAAGAG MboI 360 GATC ^GATC 862 GATC MboII 190 GAAGA GAAGA(8/7) 385 GAAGA MslI 462 CACGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 842 CACCCGGATG NaeI 15 GCCGGC GCC^GGC 51 GCCGGC NdeII 360 GATC ^GATC 862 GATC NgoMIV 15 GCCGGC G^CCGGC 51 GCCGGC PpuMI 320 GGGTCCT [GA]G^G[AT]CC[CT] 410 AGGTCCC PstI 196 CTGCAG CTGCA^G 439 CTGCAG PvuII 505 CAGCTG CAG^CTG 646 CAGCTG Sau3AI 360 GATC ^GATC 862 GATC SfaNI 718 GATGC GCATC(5/9) 365 GCATC SfcI 196 CTGCAG C^T[GA][CT]AG 439 CTGCAG Tsp45I 239 GTGAC ^GT[GC]AC 403 GTCAC TspRI 442 CAGTG CA[GC]TG[ACGT][ACGT]^ 773 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV SacI Sau96I ScrFI TseI