(Circular) (Six-base) MAPSORT of: pFN21AE4057.seq Check: 7389 from: 1 to: 5596 pFN21AE4057 5596 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2701 2701 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3659 5043 5311 1632 Size: 2027 1384 268 1917 Fragments arranged by size: 2027 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2117 2312 1184 Size: 933 195 4468 Fragments arranged by size: 4468 933 195 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2327 2359 2510 2542 2871 2903 2327 Size: 32 151 32 329 32 5020 Fragments arranged by size: 5020 329 151 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2149 3339 4911 2149 Size: 1190 1572 2834 Fragments arranged by size: 2834 1572 1190 ApaI G_GGCC'C Cuts at: 2265 2265 Size: 5596 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2921 1870 Size: 73 978 4545 Fragments arranged by size: 4545 978 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2845 2917 4372 618 Size: 325 217 627 1058 72 1455 1842 Fragments arranged by size: 1842 1455 1058 627 325 217 72 BanII G_rGCy'C Cuts at: 1391 2265 2466 2589 2615 2915 1391 Size: 874 201 123 26 300 4072 Fragments arranged by size: 4072 874 300 201 123 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BbvCI CC'TCA_GC Cuts at: 2174 2316 2174 Size: 142 5454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3787 3821 1903 Size: 34 50 34 1766 34 3678 Fragments arranged by size: 3678 1766 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 4207 136 Size: 107 122 71 631 605 373 2162 1525 Fragments arranged by size: 2162 1525 631 605 373 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 2609 2769 3013 2609 Size: 160 244 5192 Fragments arranged by size: 5192 244 160 Bme1580I G_kGCm'C Cuts at: 1790 2123 2265 3657 4813 1790 Size: 333 142 1392 1156 2573 Fragments arranged by size: 2573 1392 1156 333 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2805 2805 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2675 2858 4256 1239 Size: 1112 324 183 1398 2579 Fragments arranged by size: 2579 1398 1112 324 183 Bpu10I CC'TnA_GC Cuts at: 2174 2316 2174 Size: 142 5454 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 2482 4259 882 Size: 617 714 269 1777 2219 Fragments arranged by size: 2219 1777 714 617 269 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 2369 5590 2369 Size: 3221 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3403 3785 4420 Size: 53 83 186 936 479 1391 382 635 Cuts at: 4420 275 Size: 1451 Fragments arranged by size: 1451 1391 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 4029 4427 4701 4848 5259 2382 Size: 1647 398 274 147 411 2719 Fragments arranged by size: 2719 1647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2510 2540 1178 Size: 30 165 30 1107 30 4234 Fragments arranged by size: 4234 1107 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2471 2474 2518 2660 2672 2690 2693 Size: 1147 138 3 44 142 12 18 3 Cuts at: 2693 2702 2781 1186 Size: 9 79 4001 Fragments arranged by size: 4001 1147 142 138 79 44 18 12 9 3 3 BseYI C'CCAG_C Cuts at: 2088 4799 5295 2088 Size: 2711 496 2389 Fragments arranged by size: 2711 2389 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2729 1163 Size: 573 993 4030 Fragments arranged by size: 4030 993 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2021 2074 3046 3807 4835 1540 Size: 275 186 20 53 972 761 1028 2301 Fragments arranged by size: 2301 1028 972 761 275 186 53 20 BsiHKAI G_wGCw'C Cuts at: 2466 2589 2615 2642 3657 3742 4813 2466 Size: 123 26 27 1015 85 1071 3249 Fragments arranged by size: 3249 1071 1015 123 85 27 26 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2265 2466 2589 2615 2642 2915 Size: 399 333 142 201 123 26 27 273 Cuts at: 2915 3657 3742 4813 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 333 273 201 142 123 85 27 26 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2701 2951 844 Size: 1857 250 3489 Fragments arranged by size: 3489 1857 250 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2056 4240 4427 1700 Size: 110 210 36 2184 187 2869 Fragments arranged by size: 2869 2184 210 187 110 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 2407 1184 Size: 1223 4373 BstXI CCAn_nnnn'nTGG Cuts at: 2882 2882 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591 Size: 954 367 385 17 1452 86 12 346 Cuts at: 5591 1972 Size: 1977 Fragments arranged by size: 1977 1452 954 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2763 2763 Size: 5596 BtgI C'CryG_G Cuts at: 513 1065 2015 513 Size: 552 950 4094 Fragments arranged by size: 4094 950 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2039 487 Size: 683 56 813 4044 Fragments arranged by size: 4044 813 683 56 BtsI GCAGTG_nn' Cuts at: 2453 2784 3112 3906 3926 2453 Size: 331 328 794 20 4123 Fragments arranged by size: 4123 794 331 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 4603 809 Size: 507 1089 2198 1802 Fragments arranged by size: 2198 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2071 3043 Size: 54 1425 50 275 18 188 53 972 Cuts at: 3043 3932 5561 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 972 889 275 188 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2018 2071 3043 1537 Size: 275 206 53 972 4090 Fragments arranged by size: 4090 972 275 206 53 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 2385 3526 1353 Size: 837 195 1141 3423 Fragments arranged by size: 3423 1141 837 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2464 2587 2613 2464 Size: 123 26 5447 Fragments arranged by size: 5447 123 26 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2675 2858 3659 4256 5043 5311 Size: 393 719 324 183 801 597 787 268 Cuts at: 5311 1239 Size: 1524 Fragments arranged by size: 1524 801 787 719 597 393 324 268 183 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2186 Size: 5596 EcoO109I rG'GnC_Cy Cuts at: 2226 2326 2416 2860 2226 Size: 100 90 444 4962 Fragments arranged by size: 4962 444 100 90 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2054 2503 4743 1678 Size: 376 449 2240 2531 Fragments arranged by size: 2531 2240 449 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2189 2221 2806 Size: 32 135 32 578 32 7 32 585 Cuts at: 2806 2838 4237 4269 4311 4343 1373 Size: 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1399 585 578 135 42 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 4710 4894 1305 Size: 882 2523 184 2007 Fragments arranged by size: 2523 2007 882 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2471 2851 3554 3913 4072 518 Size: 648 1305 380 703 359 159 2042 Fragments arranged by size: 2042 1305 703 648 380 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2362 2512 2653 3012 3689 4837 5082 Size: 411 345 150 141 359 677 1148 245 Cuts at: 5082 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 411 359 345 245 150 141 NaeI GCC'GGC Cuts at: 2022 2058 2022 Size: 36 5560 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5044 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NgoMIV G'CCGG_C Cuts at: 2020 2056 2020 Size: 36 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 2071 3043 2071 Size: 972 4624 NspI r_CATG'y Cuts at: 1132 1229 2432 2954 4499 1132 Size: 97 1203 522 1545 2229 Fragments arranged by size: 2229 1545 1203 522 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PfoI T'CCnGG_A Cuts at: 2328 2328 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2510 2542 3665 3697 5178 5210 2510 Size: 32 1123 32 1481 32 2896 Fragments arranged by size: 2896 1481 1123 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2326 2416 2326 Size: 90 5506 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PspOMI G'GGCC_C Cuts at: 2261 2261 Size: 5596 PstI C_TGCA'G Cuts at: 830 2205 2448 2948 830 Size: 1375 243 500 3478 Fragments arranged by size: 3478 1375 500 243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2512 2653 2512 Size: 141 5455 RsrII CG'GwC_CG Cuts at: 2209 2209 Size: 5596 SacI G_AGCT'C Cuts at: 2466 2589 2615 2466 Size: 123 26 5447 Fragments arranged by size: 5447 123 26 SacII CC_GC'GG Cuts at: 2018 2018 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SapI GCTCTTCn'nnn_ Cuts at: 2190 2190 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2201 2444 2944 4079 4760 4951 826 Size: 219 1156 243 500 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 681 500 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 2056 1700 Size: 356 5240 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3700 4601 4863 820 Size: 197 926 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2580 3035 513 Size: 552 1515 455 3074 Fragments arranged by size: 3074 1515 552 455 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 2426 2437 3623 3640 3793 3978 Size: 1255 509 337 11 1186 17 153 185 Cuts at: 3978 4423 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1186 509 445 337 185 153 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 2168 3725 2168 Size: 1557 4039 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SanDI SexAI SfiI SfoI SrfI SwaI XcmI