Restriction map for
pFN21AE4256
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AscI AseI AsnI Asp718I
AvrII BbrPI BciVI BclI BfrI
BglII BlnI BsiWI BsmI BspDI
BspHI BspLU11I BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI EcoRI EcoRV EcoT22I FbaI
FseI HindIII KpnI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 3055 GTCTAC GT^[AC][GT]AC
AcsI 1453 AAATTC [GA]^AATT[CT]
AhdI 2035 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1995 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2170 AGCGCT AGC^GCT
ApaI 2113 GGGCCC GGGCC^C
ApoI 1453 AAATTC [GA]^AATT[CT]
Asp700I 1953 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2035 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 662 TGCGCA TGC^GCA
BamHI 3006 GGATCC G^GATCC
BfaI 2500 CTAG C^TAG
BsaAI 2185 TACGTG [CT]AC^GT[GA]
BsaBI 804 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1038 GGTCTC GGTCTC(1/5)
Bsp1407I 551 TGTACA T^GTACA
BsrBI 81 CCGCTC CCGCTC(-3/-3)
BsrGI 551 TGTACA T^GTACA
BstEII 2349 GGTGACC G^GT[ACGT]ACC
BstPI 2349 GGTGACC G^GT[ACGT]ACC
Bsu36I 1995 CCTGAGG CC^T[ACGT]AGG
Eam1105I 2035 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2170 AGCGCT AGC^GCT
Eco81I 1995 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2349 GGTGACC G^GT[ACGT]ACC
FspI 662 TGCGCA TGC^GCA
HincII 1543 GTTAAC GT[CT]^[GA]AC
HindII 1543 GTTAAC GT[CT]^[GA]AC
HpaI 1543 GTTAAC GTT^AAC
MaeI 2500 CTAG C^TAG
MamI 804 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1753 CAATTG C^AATTG
MseI 1544 TTAA T^TAA
MunI 1753 CAATTG C^AATTG
SexAI 710 ACCTGGT A^CC[AT]GGT
SgrAI 564 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 551 TGTACA T^GTACA
StuI 1523 AGGCCT AGG^CCT
Tru9I 1544 TTAA T^TAA
XmnI 1953 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspI 1033 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2401 GACTCCGTC
BbeI 377 GGCGCC GGCGC^C
1888 GGCGCC
BbsI 27 GAAGAC GAAGAC(2/6)
2481 GAAGAC
BcgI 951 CGATGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2654 GCAAGACCATCG
BpuAI 27 GAAGAC GAAGAC(2/6)
2481 GAAGAC
BsiEI 339 CGGCCG CG[GA][CT]^CG
2979 CGGCCG
BsmBI 305 GAGACG CGTCTC(1/5)
1822 GAGACG
BspMI 1003 ACCTGC ACCTGC(4/8)
3020 ACCTGC
BssHII 521 GCGCGC G^CGCGC
595 GCGCGC
BstAPI 1053 GCACCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3015 GCAGGACCTGC
EagI 339 CGGCCG C^GGCCG
2979 CGGCCG
EarI 129 CTCTTC CTCTTC(1/4)
250 CTCTTC
EclXI 339 CGGCCG C^GGCCG
2979 CGGCCG
Eco52I 339 CGGCCG C^GGCCG
2979 CGGCCG
KasI 377 GGCGCC G^GCGCC
1888 GGCGCC
Ksp632I 129 CTCTTC CTCTTC(1/4)
250 CTCTTC
KspI 147 CCGCGG CCGC^GG
265 CCGCGG
NarI 377 GGCGCC GG^CGCC
1888 GGCGCC
PflFI 1033 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2401 GACTCCGTC
SacI 826 GAGCTC GAGCT^C
970 GAGCTC
SacII 147 CCGCGG CCGC^GG
265 CCGCGG
SfoI 377 GGCGCC GGC^GCC
1888 GGCGCC
SmlI 2383 CTCAAG C^T[CT][GA]AG
2926 CTCAAG
SphI 1478 GCATGC GCATG^C
1907 GCATGC
TfiI 653 GAATC G^A[AT]TC
2731 GAATC
Tsp509I 1454 AATT ^AATT
1754 AATT
Tth111I 1033 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2401 GACTCCGTC
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# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI