(Circular) (Six-base) MAPSORT of: pFN21AE4256.seq Check: 4069 from: 1 to: 7780 pFN21AE4256 7780 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3017 3017 Size: 7780 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5590 278 Size: 53 83 186 936 4054 2468 Fragments arranged by size: 4054 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5061 5123 1937 Size: 3124 62 4594 Fragments arranged by size: 4594 3124 62 Acc65I G'GTAC_C Cuts at: 5101 5101 Size: 7780 AclI AA'CG_TT Cuts at: 1563 5907 6280 1563 Size: 4344 373 3063 Fragments arranged by size: 4344 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 2896 3949 4300 5843 7227 7495 1632 Size: 419 845 1053 351 1543 1384 268 1917 Fragments arranged by size: 1917 1543 1384 1053 845 419 351 268 AfeI AGC'GCT Cuts at: 4177 4177 Size: 7780 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7583 AflIII A'CryG_T Cuts at: 1203 6679 7656 1203 Size: 5476 977 1327 Fragments arranged by size: 5476 1327 977 AgeI A'CCGG_T Cuts at: 6611 6611 Size: 7780 AhdI GACnn_n'nnGTC Cuts at: 4045 6509 4045 Size: 2464 5316 AleI CACnn'nnGTG Cuts at: 1166 3011 1166 Size: 1845 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5015 1184 Size: 3831 3949 AlwNI CAG_nnn'CTG Cuts at: 2440 3452 3472 4423 4717 5008 5026 5523 7095 Size: 1012 20 951 294 291 18 497 1572 Cuts at: 7095 2440 Size: 3125 Fragments arranged by size: 3125 1572 1012 951 497 294 291 20 18 ApaI G_GGCC'C Cuts at: 4122 4122 Size: 7780 ApaLI G'TGCA_C Cuts at: 2461 2498 5051 5837 6993 2461 Size: 37 2553 786 1156 3248 Fragments arranged by size: 3248 2553 1156 786 37 ApoI r'AATT_y Cuts at: 1493 3458 5089 5315 5467 7576 1493 Size: 1965 1631 226 152 2109 1697 Fragments arranged by size: 2109 1965 1697 1631 226 152 AseI AT'TA_AT Cuts at: 160 1032 6334 160 Size: 872 5302 1606 Fragments arranged by size: 5302 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7780 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2279 2319 3088 3223 3441 5073 Size: 73 288 48 40 769 135 218 1632 Cuts at: 5073 5105 1870 Size: 32 4545 Fragments arranged by size: 4545 1632 769 288 218 135 73 48 40 32 BamHI G'GATC_C Cuts at: 5011 5110 5477 5011 Size: 99 367 7314 Fragments arranged by size: 7314 367 99 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2382 3092 3678 3776 3893 Size: 325 217 627 595 710 586 98 117 Cuts at: 3893 5101 6556 618 Size: 1208 1455 1842 Fragments arranged by size: 1842 1455 1208 710 627 595 586 325 217 117 98 BanII G_rGCy'C Cuts at: 1391 2835 2979 3794 3938 4122 5099 1391 Size: 1444 144 815 144 184 977 4072 Fragments arranged by size: 4072 1444 977 815 184 144 144 BbeI G_GCGC'C Cuts at: 2386 3897 2386 Size: 1511 6269 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2039 4493 928 Size: 971 140 2454 4215 Fragments arranged by size: 4215 2454 971 140 BbvCI CC'TCA_GC Cuts at: 2796 3908 4211 4844 2796 Size: 1112 303 633 5732 Fragments arranged by size: 5732 1112 633 303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2945 2979 4648 4682 5971 6005 1903 Size: 34 1008 34 1669 34 1289 34 3678 Fragments arranged by size: 3678 1669 1289 1008 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5672 6882 5672 Size: 1210 6570 BclI T'GATC_A Cuts at: 1548 1548 Size: 7780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2429 2543 2763 Size: 107 122 71 631 605 757 114 220 Cuts at: 2763 3110 3527 3851 4126 4501 6391 136 Size: 347 417 324 275 375 1890 1525 Fragments arranged by size: 1890 1525 757 631 605 417 375 347 324 275 220 122 114 107 71 BglII A'GATC_T Cuts at: 7775 7775 Size: 7780 BlpI GC'TnA_GC Cuts at: 2093 3884 4130 4179 5197 2093 Size: 1791 246 49 1018 4676 Fragments arranged by size: 4676 1791 1018 246 49 Bme1580I G_kGCm'C Cuts at: 1790 2410 2465 2502 3089 3781 4122 4219 5055 Size: 620 55 37 587 692 341 97 836 Cuts at: 5055 5841 6997 1790 Size: 786 1156 2573 Fragments arranged by size: 2573 1156 836 786 692 620 587 341 97 55 37 BmgBI CAC'GTC Cuts at: 1318 2314 4042 1318 Size: 996 1728 5056 Fragments arranged by size: 5056 1728 996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2353 4229 5511 Size: 416 53 83 399 24 936 1876 1282 Cuts at: 5511 6469 442 Size: 958 1753 Fragments arranged by size: 1876 1753 1282 958 936 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7780 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2306 2825 2993 3008 3938 4073 4787 Size: 821 246 519 168 15 930 135 714 Cuts at: 4787 4973 6440 1239 Size: 186 1467 2579 Fragments arranged by size: 2579 1467 930 821 714 519 246 186 168 135 15 Bpu10I CC'TnA_GC Cuts at: 2441 2796 3908 4211 4844 2441 Size: 355 1112 303 633 5377 Fragments arranged by size: 5377 1112 633 355 303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4373 4916 5905 6770 7068 4373 Size: 543 989 865 298 5085 Fragments arranged by size: 5085 989 865 543 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3049 6443 882 Size: 617 1550 3394 2219 Fragments arranged by size: 3394 2219 1550 617 BsaAI yAC'GTr Cuts at: 493 1204 4192 5571 493 Size: 711 2988 1379 2702 Fragments arranged by size: 2988 2702 1379 711 BsaBI GATnn'nnATC Cuts at: 2813 7774 2813 Size: 4961 2819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2383 2826 2841 3073 Size: 53 83 186 936 850 443 15 232 Cuts at: 3073 3150 3575 3830 3894 5587 5969 6604 275 Size: 77 425 255 64 1693 382 635 1451 Fragments arranged by size: 1693 1451 936 850 635 443 425 382 255 232 186 83 77 64 53 15 BsaWI w'CCGG_w Cuts at: 3426 3538 4482 6213 6611 6885 7032 7443 3426 Size: 112 944 1731 398 274 147 411 3763 Fragments arranged by size: 3763 1731 944 411 398 274 147 112 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 3029 3059 4151 Size: 30 165 30 762 30 834 30 1092 Cuts at: 4151 4181 4304 4334 4760 4790 5054 5084 1178 Size: 30 123 30 426 30 264 30 3874 Fragments arranged by size: 3874 1092 834 762 426 264 165 123 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2738 3896 3928 4070 4346 4349 4361 Size: 1117 435 1158 32 142 276 3 12 Cuts at: 4361 1186 Size: 4605 Fragments arranged by size: 4605 1158 1117 435 276 142 32 12 3 BseYI C'CCAG_C Cuts at: 2205 2505 2967 3599 3635 4466 6983 7479 2205 Size: 300 462 632 36 831 2517 496 2506 Fragments arranged by size: 2517 2506 831 632 496 462 300 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 2498 4223 4316 5021 1163 Size: 573 699 63 1725 93 705 3922 Fragments arranged by size: 3922 1725 705 699 573 93 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2347 4987 5230 5991 7019 1540 Size: 275 186 346 2640 243 761 1028 2301 Fragments arranged by size: 2640 2301 1028 761 346 275 243 186 BsiHKAI G_wGCw'C Cuts at: 2328 2465 2502 2835 2979 3312 4185 4835 5055 Size: 137 37 333 144 333 873 650 220 Cuts at: 5055 5841 5926 6997 2328 Size: 786 85 1071 3111 Fragments arranged by size: 3111 1071 873 786 650 333 333 220 144 137 85 37 BsmI GAATG_Cn' Cuts at: 5291 5384 5291 Size: 93 7687 BsmBI CGTCTCn'nnnn_ Cuts at: 2304 3821 2304 Size: 1517 6263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2410 2465 2502 2835 2979 3089 Size: 399 538 82 55 37 333 144 110 Cuts at: 3089 3312 3781 3794 3938 4122 4185 4219 4835 Size: 223 469 13 144 184 63 34 616 Cuts at: 4835 5055 5099 5841 5926 6997 1391 Size: 220 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 616 538 469 399 333 223 220 184 144 144 110 85 82 63 55 44 37 34 13 BspEI T'CCGG_A Cuts at: 3426 3538 4482 7443 3426 Size: 112 944 2961 3763 Fragments arranged by size: 3763 2961 944 112 BspHI T'CATG_A Cuts at: 5669 7399 5669 Size: 1730 6050 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3017 5034 5135 844 Size: 2173 2017 101 3489 Fragments arranged by size: 3489 2173 2017 101 BsrBI CCG'CTC Cuts at: 1394 1823 2088 5667 1394 Size: 429 265 3579 3507 Fragments arranged by size: 3579 3507 429 265 BsrDI GCAATG_nn' Cuts at: 65 1248 6275 6449 65 Size: 1183 5027 174 1396 Fragments arranged by size: 5027 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2346 2570 3702 4495 6424 6611 1700 Size: 110 536 224 1132 793 1929 187 2869 Fragments arranged by size: 2869 1929 1132 793 536 224 187 110 BsrGI T'GTAC_A Cuts at: 96 2556 96 Size: 2460 5320 BssHII G'CGCG_C Cuts at: 1925 2526 2600 1925 Size: 601 74 7105 Fragments arranged by size: 7105 601 74 BssSI C'ACGA_G Cuts at: 2080 2675 3947 5840 6852 2080 Size: 595 1272 1893 1012 3008 Fragments arranged by size: 3008 1893 1272 1012 595 BstAPI GCAn_nnn'nTGC Cuts at: 794 3064 5026 794 Size: 2270 1962 3548 Fragments arranged by size: 3548 2270 1962 BstBI TT'CG_AA Cuts at: 6584 6584 Size: 7780 BstEII G'GTnAC_C Cuts at: 1184 4354 1184 Size: 3170 4610 BstXI CCAn_nnnn'nTGG Cuts at: 2743 3013 4106 4448 2743 Size: 270 1093 342 6075 Fragments arranged by size: 6075 1093 342 270 BstYI r'GATC_y Cuts at: 1972 2727 4059 4479 4507 4746 4824 4884 5011 Size: 755 1332 420 28 239 78 60 127 Cuts at: 5011 5110 5477 5862 5879 7331 7417 7429 7775 Size: 99 367 385 17 1452 86 12 346 Cuts at: 7775 1972 Size: 1977 Fragments arranged by size: 1977 1452 1332 755 420 385 367 346 239 127 99 86 78 60 28 17 12 Bsu36I CC'TnA_GG Cuts at: 4001 4001 Size: 7780 BtgI C'CryG_G Cuts at: 513 1065 2004 2152 2270 3198 3327 3555 3925 Size: 552 939 148 118 928 129 228 370 Cuts at: 3925 3982 4203 4740 513 Size: 57 221 537 3553 Fragments arranged by size: 3553 939 928 552 537 370 228 221 148 129 118 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3626 487 Size: 683 56 2400 4641 Fragments arranged by size: 4641 2400 683 56 BtsI GCAGTG_nn' Cuts at: 3804 4111 4441 4816 5015 5296 6090 6110 3804 Size: 307 330 375 199 281 794 20 5474 Fragments arranged by size: 5474 794 375 330 307 281 199 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7780 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4500 4535 529 Size: 35 3936 35 3774 Fragments arranged by size: 3936 3774 35 35 DraI TTT'AAA Cuts at: 5084 5084 Size: 7780 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6787 809 Size: 507 5471 1802 Fragments arranged by size: 5471 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2247 2344 Size: 54 1425 50 275 18 177 240 97 Cuts at: 2344 2388 2514 2745 3003 3291 3985 4065 4497 Size: 44 126 231 258 288 694 80 432 Cuts at: 4497 4984 5227 6116 7745 8 Size: 487 243 889 1629 43 Fragments arranged by size: 1629 1425 889 694 487 432 288 275 258 243 240 231 177 126 97 80 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2344 4984 5227 1537 Size: 275 532 2640 243 4090 Fragments arranged by size: 4090 2640 532 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 2140 2261 5710 1353 Size: 787 121 3449 3423 Fragments arranged by size: 3449 3423 787 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2356 2781 6367 6741 6887 387 Size: 1064 138 767 425 3586 374 146 1280 Fragments arranged by size: 3586 1280 1064 767 425 374 146 138 EcoICRI GAG'CTC Cuts at: 2833 2977 2833 Size: 144 7636 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2060 2306 2825 2896 2993 3008 Size: 393 419 9 246 519 71 97 15 Cuts at: 3008 3938 3949 4073 4300 4787 4973 5843 6440 Size: 930 11 124 227 487 186 870 597 Cuts at: 6440 7227 7495 1239 Size: 787 268 1524 Fragments arranged by size: 1524 930 870 787 597 519 487 419 393 268 246 227 186 124 97 71 15 11 9 EcoNI CCTnn'n_nnAGG Cuts at: 2720 3427 3947 4067 4157 2720 Size: 707 520 120 90 6343 Fragments arranged by size: 6343 707 520 120 90 EcoO109I rG'GnC_Cy Cuts at: 2236 2800 2931 3469 4118 4150 4263 4945 5005 Size: 564 131 538 649 32 113 682 60 Cuts at: 5005 5023 2236 Size: 18 4993 Fragments arranged by size: 4993 682 649 564 538 131 113 60 32 18 EcoRI G'AATT_C Cuts at: 5089 5467 5089 Size: 378 7402 FspI TGC'GCA Cuts at: 2669 6286 2669 Size: 3617 4163 FspAI rTGC'GCAy Cuts at: 2669 2669 Size: 7780 HaeII r_GCGC'y Cuts at: 1678 2129 2340 2386 2440 2487 2513 2549 2571 Size: 451 211 46 54 47 26 36 22 Cuts at: 2571 3897 4179 6927 1678 Size: 1326 282 2748 2531 Fragments arranged by size: 2748 2531 1326 451 282 211 54 47 46 36 26 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 3029 3061 4031 Size: 32 135 32 671 32 754 32 970 Cuts at: 4031 4063 4240 4272 4304 4336 4699 4731 4760 Size: 32 177 32 32 32 363 32 29 Cuts at: 4760 4792 6421 6453 6495 6527 1373 Size: 32 1629 32 42 32 2626 Fragments arranged by size: 2626 1629 970 754 671 363 177 135 42 32 32 32 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 3550 5124 5370 669 Size: 988 281 1612 1574 246 3079 Fragments arranged by size: 3079 1612 1574 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7780 HpaI GTT'AAC Cuts at: 3550 5370 3550 Size: 1820 5960 KasI G'GCGC_C Cuts at: 2382 3893 2382 Size: 1511 6269 KpnI G_GTAC'C Cuts at: 5105 5105 Size: 7780 MfeI C'AATT_G Cuts at: 3758 5379 3758 Size: 1621 6159 MluI A'CGCG_T Cuts at: 7656 7656 Size: 7780 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 6894 7078 1305 Size: 834 4755 184 2007 Fragments arranged by size: 4755 2007 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 3005 3293 10 Size: 54 1425 343 915 258 288 4497 Fragments arranged by size: 4497 1425 915 343 288 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 3011 3749 4202 4446 5738 6097 6256 Size: 648 1845 738 453 244 1292 359 159 Cuts at: 6256 518 Size: 2042 Fragments arranged by size: 2042 1845 1292 738 648 453 359 244 159 MspA1I CmG'CkG Cuts at: 1606 2154 2205 2272 2505 2640 2971 3336 3841 Size: 548 51 67 233 135 331 365 505 Cuts at: 3841 3977 4781 4798 5196 5873 7021 7266 1606 Size: 136 804 17 398 677 1148 245 2120 Fragments arranged by size: 2120 1148 804 677 548 505 398 365 331 245 233 136 135 67 51 17 NaeI GCC'GGC Cuts at: 2348 2572 3704 4497 2348 Size: 224 1132 793 5631 Fragments arranged by size: 5631 1132 793 224 NarI GG'CG_CC Cuts at: 2383 3894 2383 Size: 1511 6269 NcoI C'CATG_G Cuts at: 513 1065 2004 3198 3555 4203 4740 513 Size: 552 939 1194 357 648 537 3553 Fragments arranged by size: 3553 1194 939 648 552 537 357 NdeI CA'TA_TG Cuts at: 387 387 Size: 7780 NgoMIV G'CCGG_C Cuts at: 2346 2570 3702 4495 2346 Size: 224 1132 793 5631 Fragments arranged by size: 5631 1132 793 224 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7780 NotI GC'GGCC_GC Cuts at: 5227 5227 Size: 7780 NspI r_CATG'y Cuts at: 1132 1229 3067 3487 3916 5138 6683 1132 Size: 97 1838 420 429 1222 1545 2229 Fragments arranged by size: 2229 1838 1545 1222 429 420 97 PasI CC'CwG_GG Cuts at: 1348 2935 1348 Size: 1587 6193 PciI A'CATG_T Cuts at: 6679 6679 Size: 7780 PflMI CCAn_nnn'nTGG Cuts at: 1108 3138 3472 3931 4423 1108 Size: 2030 334 459 492 4465 Fragments arranged by size: 4465 2030 492 459 334 PfoI T'CCnGG_A Cuts at: 4764 4764 Size: 7780 PmeI GTTT'AAAC Cuts at: 5084 5084 Size: 7780 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5849 5881 7362 7394 5849 Size: 32 1481 32 6235 Fragments arranged by size: 6235 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 2931 3469 4263 5023 2236 Size: 695 538 794 760 4993 Fragments arranged by size: 4993 794 760 695 538 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7780 PsiI TTA'TAA Cuts at: 5350 5350 Size: 7780 PspOMI G'GGCC_C Cuts at: 4118 4118 Size: 7780 PstI C_TGCA'G Cuts at: 830 3840 4812 5022 5031 5132 830 Size: 3010 972 210 9 101 3478 Fragments arranged by size: 3478 3010 972 210 101 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7780 PvuII CAG'CTG Cuts at: 2205 2505 2971 3841 3977 4781 4798 2205 Size: 300 466 870 136 804 17 5187 Fragments arranged by size: 5187 870 804 466 300 136 17 SacI G_AGCT'C Cuts at: 2835 2979 2835 Size: 144 7636 SacII CC_GC'GG Cuts at: 2155 2273 2155 Size: 118 7662 SalI G'TCGA_C Cuts at: 1936 5122 1936 Size: 3186 4594 SanDI GG'GwC_CC Cuts at: 2931 2931 Size: 7780 SbfI CC_TGCA'GG Cuts at: 5132 5132 Size: 7780 ScaI AGT'ACT Cuts at: 6028 7740 6028 Size: 1712 6068 SexAI A'CCwGG_T Cuts at: 2715 2715 Size: 7780 SfcI C'TryA_G Cuts at: 826 1045 3836 4154 4171 4808 5018 5027 5128 Size: 219 2791 318 17 637 210 9 101 Cuts at: 5128 6263 6944 7135 826 Size: 1135 681 191 1471 Fragments arranged by size: 2791 1471 1135 681 637 318 219 210 191 101 17 9 SfoI GGC'GCC Cuts at: 2384 3895 2384 Size: 1511 6269 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7780 SgrAI Cr'CCGG_yG Cuts at: 1700 2570 1700 Size: 870 6910 SmaI CCC'GGG Cuts at: 1872 2321 3090 5075 5107 1872 Size: 449 769 1985 32 4545 Fragments arranged by size: 4545 1985 769 449 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 4388 4931 5884 6785 7047 820 Size: 197 926 2445 543 953 901 262 1553 Fragments arranged by size: 2445 1553 953 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 5571 493 Size: 5078 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7780 SphI G_CATG'C Cuts at: 1132 3487 3916 5138 1132 Size: 2355 429 1222 3774 Fragments arranged by size: 3774 2355 1222 429 SspI AAT'ATT Cuts at: 5 52 5704 5 Size: 47 5652 2081 Fragments arranged by size: 5652 2081 47 StuI AGG'CCT Cuts at: 1517 3530 1517 Size: 2013 5767 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3198 3531 3555 4146 4203 Size: 552 939 48 1146 333 24 591 57 Cuts at: 4203 4740 5219 513 Size: 537 479 3074 Fragments arranged by size: 3074 1146 939 591 552 537 479 333 57 48 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 4373 4454 5807 5824 5977 6162 Size: 1255 738 2055 81 1353 17 153 185 Cuts at: 6162 6607 325 Size: 445 1498 Fragments arranged by size: 2055 1498 1353 1255 738 445 185 153 81 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 4167 4338 Size: 274 80 33 51 1443 579 1611 171 Cuts at: 4338 4719 6026 7738 96 Size: 381 1307 1712 138 Fragments arranged by size: 1712 1611 1443 1307 579 381 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4209 4241 4569 4601 7740 7772 4209 Size: 32 328 32 3139 32 4217 Fragments arranged by size: 4217 3139 328 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3041 4409 1316 Size: 1725 1368 4687 Fragments arranged by size: 4687 1725 1368 XbaI T'CTAG_A Cuts at: 5116 5116 Size: 7780 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2759 3529 4762 2759 Size: 770 1233 5777 Fragments arranged by size: 5777 1233 770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7780 XmaI C'CCGG_G Cuts at: 1870 2319 3088 5073 5105 1870 Size: 449 769 1985 32 4545 Fragments arranged by size: 4545 1985 769 449 32 XmnI GAAnn'nnTTC Cuts at: 3962 5909 3962 Size: 1947 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5588 276 Size: 53 83 186 936 4054 2468 Fragments arranged by size: 4054 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI