(Linear) (Six-base) MAPSORT of: KIEE4256.seq Check: 2408 from: 1 to: 3075 KIEE4256.orf With 182 enzymes: SgfI * July 3, 2012 18:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1012 3075 Size: 1012 2063 AccI GT'mk_AC Cuts at: 0 3056 3075 Size: 3056 19 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 891 1944 2295 3075 Size: 46 845 1053 351 780 Fragments arranged by size: 1053 845 780 351 46 AfeI AGC'GCT Cuts at: 0 2172 3075 Size: 2172 903 AhdI GACnn_n'nnGTC Cuts at: 0 2040 3075 Size: 2040 1035 AleI CACnn'nnGTG Cuts at: 0 1006 3075 Size: 1006 2069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3010 3075 Size: 3010 65 AlwNI CAG_nnn'CTG Cuts at: 0 435 1447 1467 2418 2712 3003 3021 3075 Size: 435 1012 20 951 294 291 18 54 Fragments arranged by size: 1012 951 435 294 291 54 20 18 ApaI G_GGCC'C Cuts at: 0 2117 3075 Size: 2117 958 ApaLI G'TGCA_C Cuts at: 0 456 493 3046 3075 Size: 456 37 2553 29 Fragments arranged by size: 2553 456 37 29 ApoI r'AATT_y Cuts at: 0 1453 3075 Size: 1453 1622 AvaI C'yCGr_G Cuts at: 0 226 274 314 1083 1218 1436 3068 3075 Size: 226 48 40 769 135 218 1632 7 Fragments arranged by size: 1632 769 226 218 135 48 40 7 BamHI G'GATC_C Cuts at: 0 3006 3075 Size: 3006 69 BanI G'GyrC_C Cuts at: 0 377 1087 1673 1771 1888 3075 Size: 377 710 586 98 117 1187 Fragments arranged by size: 1187 710 586 377 117 98 BanII G_rGCy'C Cuts at: 0 830 974 1789 1933 2117 3075 Size: 830 144 815 144 184 958 Fragments arranged by size: 958 830 815 184 144 144 BbeI G_GCGC'C Cuts at: 0 381 1892 3075 Size: 381 1511 1183 Fragments arranged by size: 1511 1183 381 BbsI GAAGACnn'nnnn_ Cuts at: 0 34 2488 3075 Size: 34 2454 587 Fragments arranged by size: 2454 587 34 BbvCI CC'TCA_GC Cuts at: 0 791 1903 2206 2839 3075 Size: 791 1112 303 633 236 Fragments arranged by size: 1112 791 633 303 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 940 974 2643 2677 3075 Size: 940 34 1669 34 398 Fragments arranged by size: 1669 940 398 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 424 538 758 1105 1522 1846 2121 2496 Size: 424 114 220 347 417 324 275 375 Cuts at: 2496 3075 Size: 579 Fragments arranged by size: 579 424 417 375 347 324 275 220 114 BlpI GC'TnA_GC Cuts at: 0 88 1879 2125 2174 3075 Size: 88 1791 246 49 901 Fragments arranged by size: 1791 901 246 88 49 Bme1580I G_kGCm'C Cuts at: 0 405 460 497 1084 1776 2117 2214 3050 Size: 405 55 37 587 692 341 97 836 Cuts at: 3050 3075 Size: 25 Fragments arranged by size: 836 692 587 405 341 97 55 37 25 BmgBI CAC'GTC Cuts at: 0 309 2037 3075 Size: 309 1728 1038 Fragments arranged by size: 1728 1038 309 BmrI ACTGGGnnnn_n' Cuts at: 0 348 2224 3075 Size: 348 1876 851 Fragments arranged by size: 1876 851 348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 301 820 988 1003 1933 2068 2782 Size: 55 246 519 168 15 930 135 714 Cuts at: 2782 2968 3075 Size: 186 107 Fragments arranged by size: 930 714 519 246 186 168 135 107 55 15 Bpu10I CC'TnA_GC Cuts at: 0 436 791 1903 2206 2839 3075 Size: 436 355 1112 303 633 236 Fragments arranged by size: 1112 633 436 355 303 236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2368 2911 3075 Size: 2368 543 164 Fragments arranged by size: 2368 543 164 BsaI GGTCTCn'nnnn_ Cuts at: 0 1044 3075 Size: 1044 2031 BsaAI yAC'GTr Cuts at: 0 2187 3075 Size: 2187 888 BsaBI GATnn'nnATC Cuts at: 0 808 3075 Size: 808 2267 BsaHI Gr'CG_yC Cuts at: 0 378 821 836 1068 1145 1570 1825 1889 Size: 378 443 15 232 77 425 255 64 Cuts at: 1889 3075 Size: 1186 Fragments arranged by size: 1186 443 425 378 255 232 77 64 15 BsaWI w'CCGG_w Cuts at: 0 1421 1533 2477 3075 Size: 1421 112 944 598 Fragments arranged by size: 1421 944 598 112 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 160 190 1024 1054 2146 2176 2299 2329 Size: 160 30 834 30 1092 30 123 30 Cuts at: 2329 2755 2785 3049 3075 Size: 426 30 264 26 Fragments arranged by size: 1092 834 426 264 160 123 30 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 298 733 1891 1923 2065 2341 2344 2356 Size: 298 435 1158 32 142 276 3 12 Cuts at: 2356 3075 Size: 719 Fragments arranged by size: 1158 719 435 298 276 142 32 12 3 BseYI C'CCAG_C Cuts at: 0 200 500 962 1594 1630 2461 3075 Size: 200 300 462 632 36 831 614 Fragments arranged by size: 831 632 614 462 300 200 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 493 2218 2311 3016 3075 Size: 430 63 1725 93 705 59 Fragments arranged by size: 1725 705 430 93 63 59 BsiEI CG_ry'CG Cuts at: 0 342 2982 3075 Size: 342 2640 93 Fragments arranged by size: 2640 342 93 BsiHKAI G_wGCw'C Cuts at: 0 323 460 497 830 974 1307 2180 2830 Size: 323 137 37 333 144 333 873 650 Cuts at: 2830 3050 3075 Size: 220 25 Fragments arranged by size: 873 650 333 333 323 220 144 137 37 25 BsmBI CGTCTCn'nnnn_ Cuts at: 0 299 1816 3075 Size: 299 1517 1259 Fragments arranged by size: 1517 1259 299 Bsp1286I G_dGCh'C Cuts at: 0 323 405 460 497 830 974 1084 1307 Size: 323 82 55 37 333 144 110 223 Cuts at: 1307 1776 1789 1933 2117 2180 2214 2830 3050 Size: 469 13 144 184 63 34 616 220 Cuts at: 3050 3075 Size: 25 Fragments arranged by size: 616 469 333 323 223 220 184 144 144 110 82 63 55 37 34 25 13 BspEI T'CCGG_A Cuts at: 0 1421 1533 2477 3075 Size: 1421 112 944 598 Fragments arranged by size: 1421 944 598 112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1012 3029 3075 Size: 1012 2017 46 Fragments arranged by size: 2017 1012 46 BsrBI CCG'CTC Cuts at: 0 83 3075 Size: 83 2992 BsrFI r'CCGG_y Cuts at: 0 341 565 1697 2490 3075 Size: 341 224 1132 793 585 Fragments arranged by size: 1132 793 585 341 224 BsrGI T'GTAC_A Cuts at: 0 551 3075 Size: 551 2524 BssHII G'CGCG_C Cuts at: 0 521 595 3075 Size: 521 74 2480 Fragments arranged by size: 2480 521 74 BssSI C'ACGA_G Cuts at: 0 75 670 1942 3075 Size: 75 595 1272 1133 Fragments arranged by size: 1272 1133 595 75 BstAPI GCAn_nnn'nTGC Cuts at: 0 1059 3021 3075 Size: 1059 1962 54 Fragments arranged by size: 1962 1059 54 BstEII G'GTnAC_C Cuts at: 0 2349 3075 Size: 2349 726 BstXI CCAn_nnnn'nTGG Cuts at: 0 738 1008 2101 2443 3075 Size: 738 270 1093 342 632 Fragments arranged by size: 1093 738 632 342 270 BstYI r'GATC_y Cuts at: 0 722 2054 2474 2502 2741 2819 2879 3006 Size: 722 1332 420 28 239 78 60 127 Cuts at: 3006 3075 Size: 69 Fragments arranged by size: 1332 722 420 239 127 78 69 60 28 Bsu36I CC'TnA_GG Cuts at: 0 1996 3075 Size: 1996 1079 BtgI C'CryG_G Cuts at: 0 147 265 1193 1322 1550 1920 1977 2198 Size: 147 118 928 129 228 370 57 221 Cuts at: 2198 2735 3075 Size: 537 340 Fragments arranged by size: 928 537 370 340 228 221 147 129 118 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1621 3075 Size: 1621 1454 BtsI GCAGTG_nn' Cuts at: 0 1799 2106 2436 2811 3010 3075 Size: 1799 307 330 375 199 65 Fragments arranged by size: 1799 375 330 307 199 65 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2495 2530 3075 Size: 2495 35 545 Fragments arranged by size: 2495 545 35 EaeI y'GGCC_r Cuts at: 0 2 242 339 383 509 740 998 1286 Size: 2 240 97 44 126 231 258 288 Cuts at: 1286 1980 2060 2492 2979 3075 Size: 694 80 432 487 96 Fragments arranged by size: 694 487 432 288 258 240 231 126 97 96 80 44 2 EagI C'GGCC_G Cuts at: 0 339 2979 3075 Size: 339 2640 96 Fragments arranged by size: 2640 339 96 EarI CTCTTCn'nnn_ Cuts at: 0 135 256 3075 Size: 135 121 2819 Fragments arranged by size: 2819 135 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 351 776 3075 Size: 351 425 2299 Fragments arranged by size: 2299 425 351 EcoICRI GAG'CTC Cuts at: 0 828 972 3075 Size: 828 144 2103 Fragments arranged by size: 2103 828 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 55 301 820 891 988 1003 1933 Size: 46 9 246 519 71 97 15 930 Cuts at: 1933 1944 2068 2295 2782 2968 3075 Size: 11 124 227 487 186 107 Fragments arranged by size: 930 519 487 246 227 186 124 107 97 71 46 15 11 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 715 1422 1942 2062 2152 3075 Size: 715 707 520 120 90 923 Fragments arranged by size: 923 715 707 520 120 90 EcoO109I rG'GnC_Cy Cuts at: 0 231 795 926 1464 2113 2145 2258 2940 Size: 231 564 131 538 649 32 113 682 Cuts at: 2940 3000 3018 3075 Size: 60 18 57 Fragments arranged by size: 682 649 564 538 231 131 113 60 57 32 18 FspI TGC'GCA Cuts at: 0 664 3075 Size: 664 2411 FspAI rTGC'GCAy Cuts at: 0 664 3075 Size: 664 2411 HaeII r_GCGC'y Cuts at: 0 124 335 381 435 482 508 544 566 Size: 124 211 46 54 47 26 36 22 Cuts at: 566 1892 2174 3075 Size: 1326 282 901 Fragments arranged by size: 1326 901 282 211 124 54 47 46 36 26 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 238 270 1024 1056 2026 2058 2235 2267 Size: 238 32 754 32 970 32 177 32 Cuts at: 2267 2299 2331 2694 2726 2755 2787 3075 Size: 32 32 363 32 29 32 288 Fragments arranged by size: 970 754 363 288 238 177 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 1545 3075 Size: 1545 1530 HpaI GTT'AAC Cuts at: 0 1545 3075 Size: 1545 1530 KasI G'GCGC_C Cuts at: 0 377 1888 3075 Size: 377 1511 1187 Fragments arranged by size: 1511 1187 377 MfeI C'AATT_G Cuts at: 0 1753 3075 Size: 1753 1322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 134 3075 Size: 134 2941 MscI TGG'CCA Cuts at: 0 742 1000 1288 3075 Size: 742 258 288 1787 Fragments arranged by size: 1787 742 288 258 MslI CAynn'nnrTG Cuts at: 0 1006 1744 2197 2441 3075 Size: 1006 738 453 244 634 Fragments arranged by size: 1006 738 634 453 244 MspA1I CmG'CkG Cuts at: 0 149 200 267 500 635 966 1331 1836 Size: 149 51 67 233 135 331 365 505 Cuts at: 1836 1972 2776 2793 3075 Size: 136 804 17 282 Fragments arranged by size: 804 505 365 331 282 233 149 136 135 67 51 17 NaeI GCC'GGC Cuts at: 0 343 567 1699 2492 3075 Size: 343 224 1132 793 583 Fragments arranged by size: 1132 793 583 343 224 NarI GG'CG_CC Cuts at: 0 378 1889 3075 Size: 378 1511 1186 Fragments arranged by size: 1511 1186 378 NcoI C'CATG_G Cuts at: 0 1193 1550 2198 2735 3075 Size: 1193 357 648 537 340 Fragments arranged by size: 1193 648 537 357 340 NgoMIV G'CCGG_C Cuts at: 0 341 565 1697 2490 3075 Size: 341 224 1132 793 585 Fragments arranged by size: 1132 793 585 341 224 NspI r_CATG'y Cuts at: 0 1062 1482 1911 3075 Size: 1062 420 429 1164 Fragments arranged by size: 1164 1062 429 420 PasI CC'CwG_GG Cuts at: 0 930 3075 Size: 930 2145 PflMI CCAn_nnn'nTGG Cuts at: 0 1133 1467 1926 2418 3075 Size: 1133 334 459 492 657 Fragments arranged by size: 1133 657 492 459 334 PfoI T'CCnGG_A Cuts at: 0 2759 3075 Size: 2759 316 PpuMI rG'GwC_Cy Cuts at: 0 231 926 1464 2258 3018 3075 Size: 231 695 538 794 760 57 Fragments arranged by size: 794 760 695 538 231 57 PspOMI G'GGCC_C Cuts at: 0 2113 3075 Size: 2113 962 PstI C_TGCA'G Cuts at: 0 1835 2807 3017 3026 3075 Size: 1835 972 210 9 49 Fragments arranged by size: 1835 972 210 49 9 PvuII CAG'CTG Cuts at: 0 200 500 966 1836 1972 2776 2793 3075 Size: 200 300 466 870 136 804 17 282 Fragments arranged by size: 870 804 466 300 282 200 136 17 SacI G_AGCT'C Cuts at: 0 830 974 3075 Size: 830 144 2101 Fragments arranged by size: 2101 830 144 SacII CC_GC'GG Cuts at: 0 150 268 3075 Size: 150 118 2807 Fragments arranged by size: 2807 150 118 SanDI GG'GwC_CC Cuts at: 0 926 3075 Size: 926 2149 SexAI A'CCwGG_T Cuts at: 0 710 3075 Size: 710 2365 SfcI C'TryA_G Cuts at: 0 1831 2149 2166 2803 3013 3022 3075 Size: 1831 318 17 637 210 9 53 Fragments arranged by size: 1831 637 318 210 53 17 9 SfoI GGC'GCC Cuts at: 0 379 1890 3075 Size: 379 1511 1185 Fragments arranged by size: 1511 1185 379 SgrAI Cr'CCGG_yG Cuts at: 0 565 3075 Size: 565 2510 SmaI CCC'GGG Cuts at: 0 316 1085 3070 3075 Size: 316 769 1985 5 Fragments arranged by size: 1985 769 316 5 SmlI C'TyrA_G Cuts at: 0 2383 2926 3075 Size: 2383 543 149 Fragments arranged by size: 2383 543 149 SphI G_CATG'C Cuts at: 0 1482 1911 3075 Size: 1482 429 1164 Fragments arranged by size: 1482 1164 429 StuI AGG'CCT Cuts at: 0 1525 3075 Size: 1525 1550 StyI C'CwwG_G Cuts at: 0 47 1193 1526 1550 2141 2198 2735 3075 Size: 47 1146 333 24 591 57 537 340 Fragments arranged by size: 1146 591 537 340 333 57 47 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 313 2368 2449 3075 Size: 313 2055 81 626 Fragments arranged by size: 2055 626 313 81 TatI w'GTAC_w Cuts at: 0 551 2162 2333 2714 3075 Size: 551 1611 171 381 361 Fragments arranged by size: 1611 551 381 361 171 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2204 2236 2564 2596 3075 Size: 2204 32 328 32 479 Fragments arranged by size: 2204 479 328 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1036 2404 3075 Size: 1036 1368 671 Fragments arranged by size: 1368 1036 671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 754 1524 2757 3075 Size: 754 770 1233 318 Fragments arranged by size: 1233 770 754 318 XmaI C'CCGG_G Cuts at: 0 314 1083 3068 3075 Size: 314 769 1985 7 Fragments arranged by size: 1985 769 314 7 XmnI GAAnn'nnTTC Cuts at: 0 1957 3075 Size: 1957 1118 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AloI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI BplI BsiWI BsmI BspHI BsrDI BstBI BstZ17I ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI HindIII KpnI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI SgfI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI