Restriction map for
pFN21AE4188
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# NO CUT ENZYMES ( 106 enzymes) #
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AccIII AclI AcsI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI Asp700I AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaAI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrFI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KasI Ksp632I
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 497 GACGTC GACGT^C
Acc65I 1795 GGTACC G^GTACC
ApaI 890 GGGCCC GGGCC^C
AseI 1786 ATTAAT AT^TAAT
AsnI 1786 ATTAAT AT^TAAT
Asp718I 1795 GGTACC G^GTACC
BanII 890 GGGCCC G[GA]GC[CT]^C
BglI 142 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1195 GCTCAGC GC^T[ACGT]AGC
BmyI 890 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 1195 GCTCAGC GC^T[ACGT]AGC
BsaBI 1032 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1363 GAATGC GAATGC(1/-1)
Bsp1286I 890 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 920 TGTACA T^GTACA
BspHI 1598 TCATGA T^CATGA
BsrBI 757 GAGCGG CCGCTC(-3/-3)
BsrGI 920 TGTACA T^GTACA
BssSI 1847 CTCGTG CACGAG(-5/-1)
BstEII 910 GGTGACC G^GT[ACGT]ACC
BstPI 910 GGTGACC G^GT[ACGT]ACC
CelII 1195 GCTCAGC GC^T[ACGT]AGC
DraIII 343 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 910 GGTGACC G^GT[ACGT]ACC
EcoRV 1588 GATATC GAT^ATC
HincII 376 GTCGAC GT[CT]^[GA]AC
HindII 376 GTCGAC GT[CT]^[GA]AC
KpnI 1795 GGTACC GGTAC^C
KspI 269 CCGCGG CCGC^GG
MamI 1032 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PshBI 1786 ATTAAT AT^TAAT
RcaI 1598 TCATGA T^CATGA
SacII 269 CCGCGG CCGC^GG
SalI 376 GTCGAC G^TCGAC
ScaI 866 AGTACT AGT^ACT
SexAI 1872 ACCTGGT A^CC[AT]GGT
SmaI 8 CCCGGG CCC^GGG
SspBI 920 TGTACA T^GTACA
SspI 1645 AATATT AAT^ATT
StuI 1806 AGGCCT AGG^CCT
XcmI 338 CCATGCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 376 GTCGAC GT^[AC][GT]AC
1606 GTCTAC
AhdI 352 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACTCTGAGTC
AspEI 352 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACTCTGAGTC
AvaI 8 CCCGGG C^[CT]CG[GA]G
1086 CTCGGG
BalI 549 TGGCCA TGG^CCA
1692 TGGCCA
BanI 933 GGTGCC G^G[CT][GA]CC
1795 GGTACC
BglII 645 AGATCT A^GATCT
1571 AGATCT
BpmI 739 CTGGAG CTGGAG(16/14)
1340 CTGGAG
BseRI 631 GAGGAG GAGGAG(10/8)
1567 GAGGAG
BsgI 516 CTGCAC GTGCAG(16/14)
730 GTGCAG
BsoBI 8 CCCGGG C^[CT]CG[GA]G
1086 CTCGGG
BsrDI 257 GCAATG GCAATG(2/0)
1535 GCAATG
BstXI 197 CCATCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1025 CCAAGGAGATGG
DsaI 48 CCGTGG C^C[GA][CT]GG
269 CCGCGG
Eam1105I 352 GACGCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACTCTGAGTC
EcoT14I 1025 CCAAGG C^C[AT][AT]GG
1652 CCTTGG
HaeII 425 AGCGCC [GA]GCGC^[CT]
2013 AGCGCC
HapII 9 CCGG C^CGG
1743 CCGG
HpaII 9 CCGG C^CGG
1743 CCGG
MluNI 549 TGGCCA TGG^CCA
1692 TGGCCA
MscI 549 TGGCCA TGG^CCA
1692 TGGCCA
MslI 198 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1281 CATGGATGTG
MspI 9 CCGG C^CGG
1743 CCGG
PflMI 660 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1005 CCAGAGACTGG
PpuMI 762 GGGTCCC [GA]G^G[AT]CC[CT]
1091 GGGACCT
PvuII 454 CAGCTG CAG^CTG
661 CAGCTG
SmlI 157 CTCAAG C^T[CT][GA]AG
529 CTCAAG
StyI 1025 CCAAGG C^C[AT][AT]GG
1652 CCTTGG
Van91I 660 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1005 CCAGAGACTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaII BbsI BbvI
BcnI BfaI BpuAI BsaHI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HgaI
HhaI Hin1I HinP1I HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII