(Circular) (Six-base) MAPSORT of: pFN21AE4188.seq Check: 7909 from: 1 to: 6727 pFN21AE4188 6727 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2506 4537 278 Size: 53 83 186 936 970 2031 2468 Fragments arranged by size: 2468 2031 970 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2382 3612 4070 1937 Size: 445 1230 458 4594 Fragments arranged by size: 4594 1230 458 445 Acc65I G'GTAC_C Cuts at: 3800 4048 3800 Size: 248 6479 AclI AA'CG_TT Cuts at: 1563 4854 5227 1563 Size: 3291 373 3063 Fragments arranged by size: 3291 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2838 3539 3554 3918 4790 6174 6442 1632 Size: 1206 701 15 364 872 1384 268 1917 Fragments arranged by size: 1917 1384 1206 872 701 364 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6530 AflIII A'CryG_T Cuts at: 1203 5626 6603 1203 Size: 4423 977 1327 Fragments arranged by size: 4423 1327 977 AgeI A'CCGG_T Cuts at: 5558 5558 Size: 6727 AhdI GACnn_n'nnGTC Cuts at: 2362 3967 5456 2362 Size: 1605 1489 3633 Fragments arranged by size: 3633 1605 1489 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6727 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3273 1184 Size: 2089 4638 AlwNI CAG_nnn'CTG Cuts at: 2671 2743 3016 3965 4470 6042 2671 Size: 72 273 949 505 1572 3356 Fragments arranged by size: 3356 1572 949 505 273 72 ApaI G_GGCC'C Cuts at: 2899 2899 Size: 6727 ApaLI G'TGCA_C Cuts at: 4784 5940 4784 Size: 1156 5571 ApoI r'AATT_y Cuts at: 1493 4036 4262 4414 6523 1493 Size: 2543 226 152 2109 1697 Fragments arranged by size: 2543 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3792 5281 160 Size: 872 2760 1489 1606 Fragments arranged by size: 2760 1606 1489 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 AvaI C'yCGr_G Cuts at: 1870 1943 2013 3091 4052 1870 Size: 73 70 1078 961 4545 Fragments arranged by size: 4545 1078 961 73 70 BamHI G'GATC_C Cuts at: 4057 4424 4057 Size: 367 6360 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2938 3800 4048 5503 618 Size: 325 217 627 1151 862 248 1455 1842 Fragments arranged by size: 1842 1455 1151 862 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2899 4046 1391 Size: 1508 1147 4072 Fragments arranged by size: 4072 1508 1147 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 2779 3556 928 Size: 971 126 754 777 4099 Fragments arranged by size: 4099 971 777 754 126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4918 4952 1903 Size: 34 50 34 2897 34 3678 Fragments arranged by size: 3678 2897 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4619 5829 4619 Size: 1210 5517 BclI T'GATC_A Cuts at: 1548 1548 Size: 6727 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2153 5338 136 Size: 107 122 71 631 605 481 3185 1525 Fragments arranged by size: 3185 1525 631 605 481 122 107 71 BglII A'GATC_T Cuts at: 2650 3576 6722 2650 Size: 926 3146 2655 Fragments arranged by size: 3146 2655 926 BlpI GC'TnA_GC Cuts at: 3201 4144 3201 Size: 943 5784 Bme1580I G_kGCm'C Cuts at: 1790 2899 4788 5944 1790 Size: 1109 1889 1156 2573 Fragments arranged by size: 2573 1889 1156 1109 BmgBI CAC'GTC Cuts at: 1318 2701 1318 Size: 1383 5344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 2766 3026 Size: 416 53 83 399 24 1158 191 260 Cuts at: 3026 3593 3614 4458 5416 442 Size: 567 21 844 958 1753 Fragments arranged by size: 1753 1158 958 844 567 416 399 260 191 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6727 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3979 3476 Size: 503 6224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 3366 5387 1239 Size: 1526 601 2021 2579 Fragments arranged by size: 2579 2021 1526 601 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 2519 4852 5717 6015 2147 Size: 372 2333 865 298 2859 Fragments arranged by size: 2859 2333 865 372 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3327 3662 3667 3774 5390 882 Size: 617 1828 335 5 107 1616 2219 Fragments arranged by size: 2219 1828 1616 617 335 107 5 BsaAI yAC'GTr Cuts at: 493 1204 4518 493 Size: 711 3314 2702 Fragments arranged by size: 3314 2702 711 BsaBI GATnn'nnATC Cuts at: 3041 6721 3041 Size: 3680 3047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2216 2358 2503 Size: 53 83 186 936 489 194 142 145 Cuts at: 2503 4534 4916 5551 275 Size: 2031 382 635 1451 Fragments arranged by size: 2031 1451 936 635 489 382 194 186 145 142 83 53 BsaWI w'CCGG_w Cuts at: 5160 5558 5832 5979 6390 5160 Size: 398 274 147 411 5497 Fragments arranged by size: 5497 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2429 2450 2459 1178 Size: 30 165 30 1017 9 21 9 5446 Fragments arranged by size: 5446 1017 165 30 30 21 9 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2651 3587 1186 Size: 1465 936 4326 Fragments arranged by size: 4326 1465 936 BseYI C'CCAG_C Cuts at: 2664 5930 6426 2664 Size: 3266 496 2965 Fragments arranged by size: 3266 2965 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2506 2756 1163 Size: 573 770 250 5134 Fragments arranged by size: 5134 770 573 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4177 4938 5966 1540 Size: 275 186 2176 761 1028 2301 Fragments arranged by size: 2301 2176 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4788 4873 5944 4788 Size: 85 1071 5571 Fragments arranged by size: 5571 1071 85 BsmI GAATG_Cn' Cuts at: 3374 4238 4331 3374 Size: 864 93 5770 Fragments arranged by size: 5770 864 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2899 4046 4788 4873 5944 1391 Size: 399 1109 1147 742 85 1071 2174 Fragments arranged by size: 2174 1147 1109 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6390 6390 Size: 6727 BspHI T'CATG_A Cuts at: 3603 4616 6346 3603 Size: 1013 1730 3984 Fragments arranged by size: 3984 1730 1013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4082 844 Size: 3238 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2764 4614 1394 Size: 429 941 1850 3507 Fragments arranged by size: 3507 1850 941 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2269 3547 5222 5396 65 Size: 1183 1021 1278 1675 174 1396 Fragments arranged by size: 1675 1396 1278 1183 1021 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5371 5558 1700 Size: 110 3561 187 2869 Fragments arranged by size: 3561 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2925 96 Size: 2829 3898 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6727 BssSI C'ACGA_G Cuts at: 3852 4787 5799 3852 Size: 935 1012 4780 Fragments arranged by size: 4780 1012 935 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6727 BstBI TT'CG_AA Cuts at: 5531 5531 Size: 6727 BstEII G'GTnAC_C Cuts at: 1184 2915 1184 Size: 1731 4996 BstXI CCAn_nnnn'nTGG Cuts at: 2209 3037 2209 Size: 828 5899 BstYI r'GATC_y Cuts at: 1972 2650 3064 3576 4057 4424 4809 4826 6278 Size: 678 414 512 481 367 385 17 1452 Cuts at: 6278 6364 6376 6722 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 678 512 481 414 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4023 4023 Size: 6727 BtgI C'CryG_G Cuts at: 513 1065 2053 2274 513 Size: 552 988 221 4966 Fragments arranged by size: 4966 988 552 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2543 487 Size: 683 56 1317 4671 Fragments arranged by size: 4671 1317 683 56 BtsI GCAGTG_nn' Cuts at: 2739 2744 3410 4243 5037 5057 2739 Size: 5 666 833 794 20 4409 Fragments arranged by size: 4409 833 794 666 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6727 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6692 DraI TTT'AAA Cuts at: 4031 4031 Size: 6727 DraIII CAC_nnn'GTG Cuts at: 2353 2353 Size: 6727 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5734 809 Size: 507 4418 1802 Fragments arranged by size: 4418 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2554 3697 Size: 54 1425 50 275 18 441 283 1143 Cuts at: 3697 4174 5063 6692 8 Size: 477 889 1629 43 Fragments arranged by size: 1629 1425 1143 889 477 441 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4174 1537 Size: 275 2362 4090 Fragments arranged by size: 4090 2362 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4657 1353 Size: 3304 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5314 5688 5834 387 Size: 1064 138 3725 374 146 1280 Fragments arranged by size: 3725 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2765 2838 3366 3539 3554 3918 4790 Size: 393 1133 73 528 173 15 364 872 Cuts at: 4790 5387 6174 6442 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1133 872 787 597 528 393 364 268 173 73 15 EcoO109I rG'GnC_Cy Cuts at: 2768 2895 2896 3097 2768 Size: 127 1 201 6398 Fragments arranged by size: 6398 201 127 1 EcoRI G'AATT_C Cuts at: 4036 4414 4036 Size: 378 6349 EcoRV GAT'ATC Cuts at: 3595 3595 Size: 6727 FspI TGC'GCA Cuts at: 5233 5233 Size: 6727 HaeII r_GCGC'y Cuts at: 1678 2434 4022 5874 1678 Size: 756 1588 1852 2531 Fragments arranged by size: 2531 1852 1588 756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 3074 3106 3953 Size: 32 135 32 776 32 694 32 847 Cuts at: 3953 3985 5368 5400 5442 5474 1373 Size: 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 847 776 694 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 4071 4317 669 Size: 988 281 445 1688 246 3079 Fragments arranged by size: 3079 1688 988 445 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6727 HpaI GTT'AAC Cuts at: 4317 4317 Size: 6727 KpnI G_GTAC'C Cuts at: 3804 4052 3804 Size: 248 6479 MfeI C'AATT_G Cuts at: 4326 4326 Size: 6727 MluI A'CGCG_T Cuts at: 6603 6603 Size: 6727 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 5841 6025 1305 Size: 1167 3369 184 2007 Fragments arranged by size: 3369 2007 1167 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2556 3699 10 Size: 54 1425 343 724 1143 3038 Fragments arranged by size: 3038 1425 1143 724 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3290 4685 5044 5203 518 Size: 648 1041 1083 1395 359 159 2042 Fragments arranged by size: 2042 1395 1083 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2226 2276 2461 2668 4143 4820 5968 6213 Size: 620 50 185 207 1475 677 1148 245 Cuts at: 6213 1606 Size: 2120 Fragments arranged by size: 2120 1475 1148 677 620 245 207 185 50 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6175 NdeI CA'TA_TG Cuts at: 387 387 Size: 6727 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6727 NotI GC'GGCC_GC Cuts at: 4174 4174 Size: 6727 NspI r_CATG'y Cuts at: 1132 1229 2283 2302 2317 2455 3487 4085 5630 Size: 97 1054 19 15 138 1032 598 1545 Cuts at: 5630 1132 Size: 2229 Fragments arranged by size: 2229 1545 1054 1032 598 138 97 19 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6727 PciI A'CATG_T Cuts at: 5626 5626 Size: 6727 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 3016 1108 Size: 1563 345 4819 Fragments arranged by size: 4819 1563 345 PmeI GTTT'AAAC Cuts at: 4031 4031 Size: 6727 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4796 4828 6309 6341 4796 Size: 32 1481 32 5182 Fragments arranged by size: 5182 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2768 3097 2768 Size: 329 6398 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6727 PsiI TTA'TAA Cuts at: 4297 4297 Size: 6727 PspOMI G'GGCC_C Cuts at: 2895 2895 Size: 6727 PstI C_TGCA'G Cuts at: 830 2146 2782 2904 2995 4079 830 Size: 1316 636 122 91 1084 3478 Fragments arranged by size: 3478 1316 1084 636 122 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6727 PvuII CAG'CTG Cuts at: 2461 2668 2461 Size: 207 6520 SacII CC_GC'GG Cuts at: 2277 2277 Size: 6727 SalI G'TCGA_C Cuts at: 1936 2381 4069 1936 Size: 445 1688 4594 Fragments arranged by size: 4594 1688 445 SanDI GG'GwC_CC Cuts at: 2768 2768 Size: 6727 SbfI CC_TGCA'GG Cuts at: 4079 4079 Size: 6727 ScaI AGT'ACT Cuts at: 2873 4975 6687 2873 Size: 2102 1712 2913 Fragments arranged by size: 2913 2102 1712 SexAI A'CCwGG_T Cuts at: 3877 3877 Size: 6727 SfcI C'TryA_G Cuts at: 826 1045 2142 2456 2778 2900 2991 3679 4075 Size: 219 1097 314 322 122 91 688 396 Cuts at: 4075 5210 5891 6082 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1097 688 681 396 322 314 219 191 122 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6727 SmaI CCC'GGG Cuts at: 1872 2015 4054 1872 Size: 143 2039 4545 Fragments arranged by size: 4545 2039 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2534 4831 5732 5994 820 Size: 197 926 219 372 2297 901 262 1553 Fragments arranged by size: 2297 1553 926 901 372 262 219 197 SnaBI TAC'GTA Cuts at: 493 4518 493 Size: 4025 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6727 SphI G_CATG'C Cuts at: 1132 4085 1132 Size: 2953 3774 SspI AAT'ATT Cuts at: 5 52 3652 4651 5 Size: 47 3600 999 2081 Fragments arranged by size: 3600 2081 999 47 StuI AGG'CCT Cuts at: 1517 3813 1517 Size: 2296 4431 StyI C'CwwG_G Cuts at: 513 1065 3030 3657 4166 513 Size: 552 1965 627 509 3074 Fragments arranged by size: 3074 1965 627 552 509 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3211 3997 4754 4771 4924 5109 5554 Size: 1255 1631 786 757 17 153 185 445 Cuts at: 5554 325 Size: 1498 Fragments arranged by size: 1631 1498 1255 786 757 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2871 2925 4973 Size: 274 80 33 51 1443 894 54 2048 Cuts at: 4973 6685 96 Size: 1712 138 Fragments arranged by size: 2048 1712 1443 894 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6687 6719 6687 Size: 32 6695 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6727 XbaI T'CTAG_A Cuts at: 4063 4063 Size: 6727 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 6727 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6727 XmaI C'CCGG_G Cuts at: 1870 2013 4052 1870 Size: 143 2039 4545 Fragments arranged by size: 4545 2039 143 XmnI GAAnn'nnTTC Cuts at: 4856 4856 Size: 6727 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2504 4535 276 Size: 53 83 186 936 970 2031 2468 Fragments arranged by size: 2468 2031 970 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI