(Linear) (Six-base) MAPSORT of: KIEE4188.seq Check: 5814 from: 1 to: 2022 KIEE4188.orf With 182 enzymes: SgfI * July 3, 2012 18:43 .. AatII G_ACGT'C Cuts at: 0 501 2022 Size: 501 1521 AccI GT'mk_AC Cuts at: 0 377 1607 2022 Size: 377 1230 415 Fragments arranged by size: 1230 415 377 Acc65I G'GTAC_C Cuts at: 0 1795 2022 Size: 1795 227 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 833 1534 1549 1913 2022 Size: 833 701 15 364 109 Fragments arranged by size: 833 701 364 109 15 AhdI GACnn_n'nnGTC Cuts at: 0 357 1962 2022 Size: 357 1605 60 Fragments arranged by size: 1605 357 60 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1268 2022 Size: 1268 754 AlwNI CAG_nnn'CTG Cuts at: 0 666 738 1011 1960 2022 Size: 666 72 273 949 62 Fragments arranged by size: 949 666 273 72 62 ApaI G_GGCC'C Cuts at: 0 894 2022 Size: 894 1128 AseI AT'TA_AT Cuts at: 0 1787 2022 Size: 1787 235 AvaI C'yCGr_G Cuts at: 0 8 1086 2022 Size: 8 1078 936 Fragments arranged by size: 1078 936 8 BanI G'GyrC_C Cuts at: 0 933 1795 2022 Size: 933 862 227 Fragments arranged by size: 933 862 227 BanII G_rGCy'C Cuts at: 0 894 2022 Size: 894 1128 BbsI GAAGACnn'nnnn_ Cuts at: 0 20 774 1551 2022 Size: 20 754 777 471 Fragments arranged by size: 777 754 471 20 BglI GCCn_nnn'nGGC Cuts at: 0 148 2022 Size: 148 1874 BglII A'GATC_T Cuts at: 0 645 1571 2022 Size: 645 926 451 Fragments arranged by size: 926 645 451 BlpI GC'TnA_GC Cuts at: 0 1196 2022 Size: 1196 826 Bme1580I G_kGCm'C Cuts at: 0 894 2022 Size: 894 1128 BmgBI CAC'GTC Cuts at: 0 696 2022 Size: 696 1326 BmrI ACTGGGnnnn_n' Cuts at: 0 570 761 1021 1588 1609 2022 Size: 570 191 260 567 21 413 Fragments arranged by size: 570 567 413 260 191 21 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1471 1974 2022 Size: 1471 503 48 Fragments arranged by size: 1471 503 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 1361 2022 Size: 760 601 661 Fragments arranged by size: 760 661 601 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 514 2022 Size: 142 372 1508 Fragments arranged by size: 1508 372 142 BsaI GGTCTCn'nnnn_ Cuts at: 0 1322 1657 1662 1769 2022 Size: 1322 335 5 107 253 Fragments arranged by size: 1322 335 253 107 5 BsaBI GATnn'nnATC Cuts at: 0 1036 2022 Size: 1036 986 BsaHI Gr'CG_yC Cuts at: 0 17 211 353 498 2022 Size: 17 194 142 145 1524 Fragments arranged by size: 1524 194 145 142 17 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 415 424 445 454 2022 Size: 415 9 21 9 1568 Fragments arranged by size: 1568 415 21 9 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 646 1582 2022 Size: 646 936 440 Fragments arranged by size: 936 646 440 BseYI C'CCAG_C Cuts at: 0 659 2022 Size: 659 1363 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 501 751 2022 Size: 501 250 1271 Fragments arranged by size: 1271 501 250 BsmI GAATG_Cn' Cuts at: 0 1369 2022 Size: 1369 653 Bsp1286I G_dGCh'C Cuts at: 0 894 2022 Size: 894 1128 BspHI T'CATG_A Cuts at: 0 1598 2022 Size: 1598 424 BsrBI CCG'CTC Cuts at: 0 759 2022 Size: 759 1263 BsrDI GCAATG_nn' Cuts at: 0 264 1542 2022 Size: 264 1278 480 Fragments arranged by size: 1278 480 264 BsrGI T'GTAC_A Cuts at: 0 920 2022 Size: 920 1102 BssSI C'ACGA_G Cuts at: 0 1847 2022 Size: 1847 175 BstEII G'GTnAC_C Cuts at: 0 910 2022 Size: 910 1112 BstXI CCAn_nnnn'nTGG Cuts at: 0 204 1032 2022 Size: 204 828 990 Fragments arranged by size: 990 828 204 BstYI r'GATC_y Cuts at: 0 645 1059 1571 2022 Size: 645 414 512 451 Fragments arranged by size: 645 512 451 414 BtgI C'CryG_G Cuts at: 0 48 269 2022 Size: 48 221 1753 Fragments arranged by size: 1753 221 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 538 2022 Size: 538 1484 BtsI GCAGTG_nn' Cuts at: 0 734 739 1405 2022 Size: 734 5 666 617 Fragments arranged by size: 734 666 617 5 DraIII CAC_nnn'GTG Cuts at: 0 348 2022 Size: 348 1674 EaeI y'GGCC_r Cuts at: 0 266 549 1692 2022 Size: 266 283 1143 330 Fragments arranged by size: 1143 330 283 266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 833 1361 1534 1549 1913 2022 Size: 760 73 528 173 15 364 109 Fragments arranged by size: 760 528 364 173 109 73 15 EcoO109I rG'GnC_Cy Cuts at: 0 763 890 891 1092 2022 Size: 763 127 1 201 930 Fragments arranged by size: 930 763 201 127 1 EcoRV GAT'ATC Cuts at: 0 1590 2022 Size: 1590 432 HaeII r_GCGC'y Cuts at: 0 429 2017 2022 Size: 429 1588 5 Fragments arranged by size: 1588 429 5 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 343 375 1069 1101 1948 1980 2022 Size: 343 32 694 32 847 32 42 Fragments arranged by size: 847 694 343 42 32 32 32 HincII GTy'rAC Cuts at: 0 378 2022 Size: 378 1644 KpnI G_GTAC'C Cuts at: 0 1799 2022 Size: 1799 223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 467 2022 Size: 467 1555 MscI TGG'CCA Cuts at: 0 551 1694 2022 Size: 551 1143 328 Fragments arranged by size: 1143 551 328 MslI CAynn'nnrTG Cuts at: 0 202 1285 2022 Size: 202 1083 737 Fragments arranged by size: 1083 737 202 MspA1I CmG'CkG Cuts at: 0 221 271 456 663 2022 Size: 221 50 185 207 1359 Fragments arranged by size: 1359 221 207 185 50 NspI r_CATG'y Cuts at: 0 278 297 312 450 1482 2022 Size: 278 19 15 138 1032 540 Fragments arranged by size: 1032 540 278 138 19 15 PflMI CCAn_nnn'nTGG Cuts at: 0 666 1011 2022 Size: 666 345 1011 Fragments arranged by size: 1011 666 345 PpuMI rG'GwC_Cy Cuts at: 0 763 1092 2022 Size: 763 329 930 Fragments arranged by size: 930 763 329 PspOMI G'GGCC_C Cuts at: 0 890 2022 Size: 890 1132 PstI C_TGCA'G Cuts at: 0 141 777 899 990 2022 Size: 141 636 122 91 1032 Fragments arranged by size: 1032 636 141 122 91 PvuII CAG'CTG Cuts at: 0 456 663 2022 Size: 456 207 1359 Fragments arranged by size: 1359 456 207 SacII CC_GC'GG Cuts at: 0 272 2022 Size: 272 1750 SalI G'TCGA_C Cuts at: 0 376 2022 Size: 376 1646 SanDI GG'GwC_CC Cuts at: 0 763 2022 Size: 763 1259 ScaI AGT'ACT Cuts at: 0 868 2022 Size: 868 1154 SexAI A'CCwGG_T Cuts at: 0 1872 2022 Size: 1872 150 SfcI C'TryA_G Cuts at: 0 137 451 773 895 986 1674 2022 Size: 137 314 322 122 91 688 348 Fragments arranged by size: 688 348 322 314 137 122 91 SmaI CCC'GGG Cuts at: 0 10 2022 Size: 10 2012 SmlI C'TyrA_G Cuts at: 0 157 529 2022 Size: 157 372 1493 Fragments arranged by size: 1493 372 157 SspI AAT'ATT Cuts at: 0 1647 2022 Size: 1647 375 StuI AGG'CCT Cuts at: 0 1808 2022 Size: 1808 214 StyI C'CwwG_G Cuts at: 0 1025 1652 2022 Size: 1025 627 370 Fragments arranged by size: 1025 627 370 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1206 1992 2022 Size: 1206 786 30 Fragments arranged by size: 1206 786 30 TatI w'GTAC_w Cuts at: 0 866 920 2022 Size: 866 54 1102 Fragments arranged by size: 1102 866 54 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 345 2022 Size: 345 1677 XmaI C'CCGG_G Cuts at: 0 8 2022 Size: 8 2014 ZraI GAC'GTC Cuts at: 0 499 2022 Size: 499 1523 Enzymes that do cut: AatII AccI Acc65I AcuI AhdI AlfI AlwNI ApaI AseI AvaI BanI BanII BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NspI PflMI PpuMI PspOMI PstI PvuII SacII SalI SanDI ScaI SexAI SfcI SmaI SmlI SspI StuI StyI TaqII TatI XcmI XmaI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AflIII AgeI AleI AloI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmtI Bpu10I BsaAI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI XmnI