Restriction map for
pFN21AE4101
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 457 GTAGAC GT^[AC][GT]AC
AflIII 303 ACATGT A^C[GA][CT]GT
AlwI 1157 GATCC GGATC(4/5)
AseI 378 ATTAAT AT^TAAT
AsnI 378 ATTAAT AT^TAAT
AspHI 1060 GTGCTC G[AT]GC[AT]^C
BalI 1074 TGGCCA TGG^CCA
BanII 821 GAGCCC G[GA]GC[CT]^C
BbsI 1051 GAAGAC GAAGAC(2/6)
BfaI 466 CTAG C^TAG
BpmI 348 CTGGAG CTGGAG(16/14)
BpuAI 1051 GAAGAC GAAGAC(2/6)
BsiHKAI 1060 GTGCTC G[AT]GC[AT]^C
BsmBI 24 GAGACG CGTCTC(1/5)
BsmI 1224 GCATTC GAATGC(1/-1)
BspLU11I 303 ACATGT A^CATGT
BsrDI 803 GCAATG GCAATG(2/0)
BssKI 1160 CCTGG ^CC[ACGT]GG
BstNI 1160 CCTGG CC^[AT]GG
BstYI 1156 AGATCC [GA]^GATC[CT]
DraI 130 TTTAAA TTT^AAA
EaeI 1074 TGGCCA [CT]^GGCC[GA]
EcoRII 1160 CCTGG ^CC[AT]GG
MaeI 466 CTAG C^TAG
MflI 1156 AGATCC [GA]^GATC[CT]
MluNI 1074 TGGCCA TGG^CCA
MscI 1074 TGGCCA TGG^CCA
MslI 304 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1111 CAGCTG C[AC]G^C[GT]G
MvaI 1160 CCTGG CC^[AT]GG
NheI 465 GCTAGC G^CTAGC
NspI 303 ACATGT [GA]CATG^[CT]
PacI 379 TTAATTAA TTAAT^TAA
PleI 1092 GAGTC GAGTC(4/5)
PpuMI 53 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 378 ATTAAT AT^TAAT
PvuII 1111 CAGCTG CAG^CTG
ScrFI 1160 CCTGG CC^[ACGT]GG
SspI 107 AATATT AAT^ATT
XhoII 1156 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 1178 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1349 CAGTTACTG
AvaII 54 GGACC G^G[AT]CC
672 GGACC
BbvI 1110 GCAGC GCAGC(8/12)
30 GCTGC
BmyI 821 GAGCCC G[AGT]GC[ACT]^C
1060 GTGCTC
BsgI 588 CTGCAC GTGCAG(16/14)
1108 GTGCAG
BsmAI 24 GAGAC GTCTC(1/5)
1391 GTCTC
Bsp1286I 821 GAGCCC G[AGT]GC[ACT]^C
1060 GTGCTC
BspHI 528 TCATGA T^CATGA
893 TCATGA
BspMI 152 ACCTGC ACCTGC(4/8)
453 GCAGGT
DraII 17 AGGGCCT [GA]G^G[ACGT]CC[CT]
53 GGGACCT
DrdI 622 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
842 GACATTATAGTC
DsaI 113 CCATGG C^C[GA][CT]GG
1127 CCATGG
EcoO109I 17 AGGGCCT [GA]G^G[ACGT]CC[CT]
53 GGGACCT
Fnu4HI 30 GCTGC GC^[ACGT]GC
1110 GCAGC
HaeIII 19 GGCC GG^CC
1075 GGCC
HphI 744 GGTGA GGTGA(8/7)
237 TCACC
ItaI 30 GCTGC GC^[ACGT]GC
1110 GCAGC
MaeIII 904 GTAAC ^GT[ACGT]AC
1351 GTTAC
MwoI 469 GCCTATATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
598 GCAATTTCAGC
NcoI 113 CCATGG C^CATGG
1127 CCATGG
RcaI 528 TCATGA T^CATGA
893 TCATGA
SmlI 314 CTCAAG C^T[CT][GA]AG
1103 CTCAAG
TaqI 8 TCGA T^CGA
936 TCGA
TseI 30 GCTGC G^C[AT]GC
1110 GCAGC
TthHB8I 8 TCGA T^CGA
936 TCGA
XcmI 211 CCAATTCGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1118 CCAACAGAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaJI
BsiYI BslI BsmFI BsrI BstF5I
Cac8I Cfr13I DdeI DpnII EcoT14I
FokI HincII HindII HinfI MboI
MboII MnlI MseI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfaNI
StyI TfiI Tru9I Tsp509I TspRI