(Circular) (Six-base) MAPSORT of: pFN21AE4101.seq Check: 9406 from: 1 to: 6118 pFN21AE4101 6118 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2463 3461 1937 Size: 526 998 4594 Fragments arranged by size: 4594 998 526 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2514 3003 3342 4181 5565 5833 1632 Size: 882 489 339 839 1384 268 1917 Fragments arranged by size: 1917 1384 882 839 489 339 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 2308 5017 5994 1203 Size: 1105 2709 977 1327 Fragments arranged by size: 2709 1327 1105 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 3188 3359 3861 5433 3188 Size: 171 502 1572 3873 Fragments arranged by size: 3873 1572 502 171 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2164 2405 2512 3427 3653 3805 5914 1493 Size: 671 241 107 915 226 152 2109 1697 Fragments arranged by size: 2109 1697 915 671 241 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 2384 4672 160 Size: 872 1352 2288 1606 Fragments arranged by size: 2288 1606 1352 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3439 4894 618 Size: 325 217 627 1652 1455 1842 Fragments arranged by size: 1842 1652 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2830 3437 1391 Size: 1439 607 4072 Fragments arranged by size: 4072 1439 607 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3063 928 Size: 971 1164 3983 Fragments arranged by size: 3983 1164 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 4179 5335 1790 Size: 2389 1156 2573 Fragments arranged by size: 2573 2389 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2474 1056 Size: 1418 4700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2374 4778 1239 Size: 1135 2404 2579 Fragments arranged by size: 2579 2404 1135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2304 3093 4243 5108 5406 2304 Size: 789 1150 865 298 3016 Fragments arranged by size: 3016 1150 865 789 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6118 BseYI C'CCAG_C Cuts at: 2523 2836 5321 5817 2523 Size: 313 2485 496 2824 Fragments arranged by size: 2824 2485 496 313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2578 3134 1163 Size: 573 842 556 4147 Fragments arranged by size: 4147 842 573 556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3069 4179 4264 5335 3069 Size: 1110 85 1071 3852 Fragments arranged by size: 3852 1110 1071 85 BsmI GAATG_Cn' Cuts at: 3229 3629 3722 3229 Size: 400 93 5625 Fragments arranged by size: 5625 400 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2023 2023 Size: 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2830 3069 3437 4179 4264 5335 1391 Size: 399 1040 239 368 742 85 1071 2174 Fragments arranged by size: 2174 1071 1040 742 399 368 239 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 2533 2898 4007 5737 2533 Size: 365 1109 1730 2914 Fragments arranged by size: 2914 1730 1109 365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2449 3473 844 Size: 1322 283 1024 3489 Fragments arranged by size: 3489 1322 1024 283 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2815 4613 4787 65 Size: 1183 1567 1798 174 1396 Fragments arranged by size: 1798 1567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3161 3448 3815 4200 4217 5669 5755 5767 Size: 1189 287 367 385 17 1452 86 12 Cuts at: 5767 6113 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1189 385 367 346 287 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 3132 513 Size: 552 939 114 1014 3499 Fragments arranged by size: 3499 1014 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 3382 3634 4428 4448 3382 Size: 252 794 20 5052 Fragments arranged by size: 5052 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 2137 3422 2137 Size: 1285 4833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2633 2853 5125 809 Size: 507 1317 220 2272 1802 Fragments arranged by size: 2272 1802 1317 507 220 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3079 3565 4454 Size: 54 1425 50 275 18 1249 486 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1249 889 486 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4048 1353 Size: 2695 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2374 2514 3003 3342 4181 4778 5565 Size: 393 742 140 489 339 839 597 787 Cuts at: 5565 5833 1239 Size: 268 1524 Fragments arranged by size: 1524 839 787 742 597 489 393 339 268 140 EcoO109I rG'GnC_Cy Cuts at: 2023 2059 2023 Size: 36 6082 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2435 3309 2435 Size: 874 5244 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2817 2849 4759 4791 4833 Size: 32 135 32 1245 32 1910 32 42 Cuts at: 4833 4865 1373 Size: 32 2626 Fragments arranged by size: 2626 1910 1245 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2764 2858 3199 3462 3708 669 Size: 988 281 826 94 341 263 246 3079 Fragments arranged by size: 3079 988 826 341 281 263 246 94 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2281 2359 2548 3147 3154 3424 5232 5416 Size: 976 78 189 599 7 270 1808 184 Cuts at: 5416 1305 Size: 2007 Fragments arranged by size: 2007 1808 976 599 270 189 184 78 7 MscI TGG'CCA Cuts at: 10 64 1489 1832 3081 10 Size: 54 1425 343 1249 3047 Fragments arranged by size: 3047 1425 1249 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 4076 4435 4594 518 Size: 648 1147 1763 359 159 2042 Fragments arranged by size: 2042 1763 1147 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3118 3534 4211 5359 5604 1606 Size: 1512 416 677 1148 245 2120 Fragments arranged by size: 2120 1512 1148 677 416 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2118 3132 513 Size: 552 939 114 1014 3499 Fragments arranged by size: 3499 1014 939 552 114 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 2470 1052 Size: 1418 4700 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 2312 3476 5021 1132 Size: 97 1083 1164 1545 2229 Fragments arranged by size: 2229 1545 1164 1083 97 PacI TTA_AT'TAA Cuts at: 2388 2388 Size: 6118 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 2308 5017 2308 Size: 2709 3409 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2059 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 3118 3118 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 3466 4601 5282 5473 826 Size: 219 2421 1135 681 191 1471 Fragments arranged by size: 2421 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2319 3108 4222 5123 5385 820 Size: 197 926 376 789 1114 901 262 1553 Fragments arranged by size: 1553 1114 926 901 789 376 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 2114 4042 5 Size: 47 2062 1928 2081 Fragments arranged by size: 2081 2062 1928 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 2742 3132 3301 3557 513 Size: 552 939 114 624 390 169 256 3074 Fragments arranged by size: 3074 939 624 552 390 256 169 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4145 4162 4315 4500 4945 325 Size: 1255 2565 17 153 185 445 1498 Fragments arranged by size: 2565 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2046 2924 4364 Size: 274 80 33 51 1443 69 878 1440 Cuts at: 4364 6076 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1440 878 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2295 2327 6078 6110 2295 Size: 32 3751 32 2303 Fragments arranged by size: 3751 2303 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2223 3130 2223 Size: 907 5211 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI