################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MfeI MluI MseI MunI NdeI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 106 GACGTC GACGT^C AccIII 995 TCCGGA T^CCGGA AfaI 837 GTAC GT^AC AflIII 839 ACGTGT A^C[GA][CT]GT AlwI 1011 GATCC GGATC(4/5) AlwNI 1041 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 386 AGCGCT AGC^GCT Asp700I 858 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 103 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 985 GGCGCC GGCGC^C BfaI 644 CTAG C^TAG BglI 168 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 838 TACGTG [CT]AC^GT[GA] BsaBI 696 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 23 GGTCTC GGTCTC(1/5) BsaWI 995 TCCGGA [AT]^CCGG[AT] BseAI 995 TCCGGA T^CCGGA BsiWI 836 CGTACG C^GTACG BspEI 995 TCCGGA T^CCGGA BsrBI 271 GAGCGG CCGCTC(-3/-3) BssSI 67 CACGAG CACGAG(-5/-1) BstYI 1010 AGATCC [GA]^GATC[CT] EarI 670 GAAGAG CTCTTC(1/4) Eco47III 386 AGCGCT AGC^GCT HinfI 40 GATTC G^A[ACGT]TC HphI 585 TCACC GGTGA(8/7) KasI 985 GGCGCC G^GCGCC Ksp632I 670 GAAGAG CTCTTC(1/4) MaeI 644 CTAG C^TAG MamI 696 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1010 AGATCC [GA]^GATC[CT] MroI 995 TCCGGA T^CCGGA NarI 985 GGCGCC GG^CGCC NcoI 773 CCATGG C^CATGG NheI 643 GCTAGC G^CTAGC PflFI 103 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1038 CTGCAG CTGCA^G RsaI 837 GTAC GT^AC SexAI 323 ACCTGGT A^CC[AT]GGT SfcI 1038 CTGCAG C^T[GA][CT]AG SfoI 985 GGCGCC GGC^GCC SmlI 523 CTCAAG C^T[CT][GA]AG TfiI 40 GATTC G^A[AT]TC Tth111I 103 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1010 AGATCC [GA]^GATC[CT] XmnI 858 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 106 GACGTC G[GA]^CG[CT]C 985 GGCGCC AspHI 315 GAGCTC G[AT]GC[AT]^C 399 GAGCTC AvaI 919 CTCGGG C^[CT]CG[GA]G 1120 CCCGAG AvaII 48 GGACC G^G[AT]CC 233 GGTCC BalI 59 TGGCCA TGG^CCA 494 TGGCCA BpmI 396 CTGGAG CTGGAG(16/14) 847 CTGGAG BsaHI 106 GACGTC G[GA]^CG[CT]C 985 GGCGCC BsiHKAI 315 GAGCTC G[AT]GC[AT]^C 399 GAGCTC BsmAI 999 GAGAC GTCTC(1/5) 24 GTCTC BsoBI 919 CTCGGG C^[CT]CG[GA]G 1120 CCCGAG BspMI 451 ACCTGC ACCTGC(4/8) 587 ACCTGC BstAPI 321 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1040 GCAGCAGCTGC BstXI 629 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 701 CCATCCCCGTGG DdeI 44 CTGAG C^T[ACGT]AG 479 CTCAG DpnII 696 GATC ^GATC 1011 GATC DraIII 322 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1015 CACGCGGTG Hin1I 106 GACGTC G[GA]^CG[CT]C 985 GGCGCC MaeII 107 ACGT A^CGT 839 ACGT MaeIII 65 GTCAC ^GT[ACGT]AC 584 GTCAC MboI 696 GATC ^GATC 1011 GATC MluNI 59 TGGCCA TGG^CCA 494 TGGCCA MscI 59 TGGCCA TGG^CCA 494 TGGCCA NaeI 160 GCCGGC GCC^GGC 942 GCCGGC NdeII 696 GATC ^GATC 1011 GATC NgoMIV 160 GCCGGC G^CCGGC 942 GCCGGC PflMI 51 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1014 CCACGCGGTGG SacI 315 GAGCTC GAGCT^C 399 GAGCTC Sau3AI 696 GATC ^GATC 1011 GATC Tsp45I 65 GTCAC ^GT[GC]AC 584 GTCAC TspRI 77 CAGTG CA[GC]TG[ACGT][ACGT]^ 229 CACTG Van91I 51 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1014 CCACGCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AluI ApaI AscI BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI KspI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV NotI PvuII SacII Sau96I ScrFI SfaNI SgrAI StyI TseI