(Circular) (Six-base) MAPSORT of: pFN21AE4073.seq Check: 2957 from: 1 to: 5851 pFN21AE4073 5851 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2465 2601 2465 Size: 136 5715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2115 3661 278 Size: 53 83 186 936 579 1546 2468 Fragments arranged by size: 2468 1546 936 579 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2380 2548 3914 5298 5566 1632 Size: 748 168 1366 1384 268 1917 Fragments arranged by size: 1917 1384 1366 748 268 168 AfeI AGC'GCT Cuts at: 2393 2393 Size: 5851 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 2844 4750 5727 1203 Size: 1641 1906 977 1327 Fragments arranged by size: 1906 1641 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 3051 3594 5166 3051 Size: 543 1572 3736 Fragments arranged by size: 3736 1572 543 ApaI G_GGCC'C Cuts at: 2673 2811 2908 2673 Size: 138 97 5616 Fragments arranged by size: 5616 138 97 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3160 3386 3538 5647 1493 Size: 1667 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 AscI GG'CGCG_CC Cuts at: 2745 2821 3030 2745 Size: 76 209 5566 Fragments arranged by size: 5566 209 76 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2924 3125 3176 1870 Size: 73 981 201 51 4545 Fragments arranged by size: 4545 981 201 73 51 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 2990 3120 3172 4627 Size: 325 217 627 466 737 130 52 1455 Cuts at: 4627 618 Size: 1842 Fragments arranged by size: 1842 1455 737 627 466 325 217 130 52 BanII G_rGCy'C Cuts at: 1391 2324 2408 2673 2811 2889 2908 2925 3170 Size: 933 84 265 138 78 19 17 245 Cuts at: 3170 1391 Size: 4072 Fragments arranged by size: 4072 933 265 245 138 84 78 19 17 BbeI G_GCGC'C Cuts at: 2994 2994 Size: 5851 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2179 4462 136 Size: 107 122 71 631 605 507 2283 1525 Fragments arranged by size: 2283 1525 631 605 507 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 3268 3268 Size: 5851 Bme1580I G_kGCm'C Cuts at: 1790 2546 2673 2811 2877 2908 3125 3912 5068 Size: 756 127 138 66 31 217 787 1156 Cuts at: 5068 1790 Size: 2573 Fragments arranged by size: 2573 1156 787 756 217 138 127 66 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 2143 3582 Size: 416 53 83 399 24 658 68 1439 Cuts at: 3582 4540 442 Size: 958 1753 Fragments arranged by size: 1753 1439 958 658 416 399 83 68 53 24 BmtI G_CTAG'C Cuts at: 1056 2652 1056 Size: 1596 4255 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2422 2873 4511 1239 Size: 1183 451 1638 2579 Fragments arranged by size: 2579 1638 1183 451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 3976 4841 5139 2513 Size: 1463 865 298 3225 Fragments arranged by size: 3225 1463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2034 4514 882 Size: 617 535 2480 2219 Fragments arranged by size: 2480 2219 617 535 BsaAI yAC'GTr Cuts at: 493 1204 2845 3642 493 Size: 711 1641 797 2702 Fragments arranged by size: 2702 1641 797 711 BsaBI GATnn'nnATC Cuts at: 2705 5845 2705 Size: 3140 2711 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2991 3658 4040 Size: 53 83 186 936 579 879 667 382 Cuts at: 4040 4675 275 Size: 635 1451 Fragments arranged by size: 1451 936 879 667 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3000 4284 4682 4956 5103 5514 3000 Size: 1284 398 274 147 411 3337 Fragments arranged by size: 3337 1284 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5626 Fragments arranged by size: 5626 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2957 3098 3101 1186 Size: 1771 141 3 3936 Fragments arranged by size: 3936 1771 141 3 BseYI C'CCAG_C Cuts at: 2117 2161 2907 5054 5550 2117 Size: 44 746 2147 496 2418 Fragments arranged by size: 2418 2147 746 496 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2585 3005 1163 Size: 573 618 231 420 4009 Fragments arranged by size: 4009 618 573 420 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2183 2189 2473 2557 2769 2816 Size: 275 186 182 6 284 84 212 47 Cuts at: 2816 3301 4062 5090 1540 Size: 485 761 1028 2301 Fragments arranged by size: 2301 1028 761 485 284 275 212 186 182 84 47 6 BsiHKAI G_wGCw'C Cuts at: 2324 2408 3912 3997 5068 2324 Size: 84 1504 85 1071 3107 Fragments arranged by size: 3107 1504 1071 85 84 BsiWI C'GTAC_G Cuts at: 2841 2841 Size: 5851 BsmI GAATG_Cn' Cuts at: 3362 3455 3362 Size: 93 5758 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2324 2408 2546 2673 2811 2877 2889 Size: 399 534 84 138 127 138 66 12 Cuts at: 2889 2908 2925 3125 3170 3912 3997 5068 1391 Size: 19 17 200 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 534 399 200 138 138 127 85 84 66 45 19 17 12 BspEI T'CCGG_A Cuts at: 3000 5514 3000 Size: 2514 3337 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2465 2601 3206 844 Size: 1621 136 605 3489 Fragments arranged by size: 3489 1621 605 136 BsrBI CCG'CTC Cuts at: 1394 1823 2278 3738 1394 Size: 429 455 1460 3507 Fragments arranged by size: 3507 1460 455 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 2270 2340 2508 2947 4495 4682 Size: 110 355 105 70 168 439 1548 187 Cuts at: 4682 1700 Size: 2869 Fragments arranged by size: 2869 1548 439 355 187 168 110 105 70 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 2745 2821 3030 1925 Size: 820 76 209 4746 Fragments arranged by size: 4746 820 209 76 BssSI C'ACGA_G Cuts at: 2072 3911 4923 2072 Size: 1839 1012 3000 Fragments arranged by size: 3000 1839 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2332 3051 794 Size: 1538 719 3594 Fragments arranged by size: 3594 1538 719 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2713 2641 Size: 72 5779 BstYI r'GATC_y Cuts at: 1972 3015 3181 3548 3933 3950 5402 5488 5500 Size: 1043 166 367 385 17 1452 86 12 Cuts at: 5500 5846 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1043 385 367 346 166 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2183 2557 2712 2763 2769 2778 Size: 552 939 179 374 155 51 6 9 Cuts at: 2778 2802 3108 3133 513 Size: 24 306 25 3231 Fragments arranged by size: 3231 939 552 374 306 179 155 51 25 24 9 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2733 487 Size: 683 56 1507 3605 Fragments arranged by size: 3605 1507 683 56 BtsI GCAGTG_nn' Cuts at: 3367 4161 4181 3367 Size: 794 20 5037 Fragments arranged by size: 5037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2197 2232 529 Size: 35 1633 35 4148 Fragments arranged by size: 4148 1633 35 35 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DraIII CAC_nnn'GTG Cuts at: 2332 3025 2332 Size: 693 5158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2180 2186 Size: 54 1425 50 275 18 234 116 6 Cuts at: 2186 2470 2499 2554 2631 2766 3298 4187 5816 Size: 284 29 55 77 135 532 889 1629 Cuts at: 5816 8 Size: 43 Fragments arranged by size: 1629 1425 889 532 284 275 234 135 116 77 55 54 50 43 29 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2180 2186 2470 2554 2766 3298 1537 Size: 275 368 6 284 84 212 532 4090 Fragments arranged by size: 4090 532 368 284 275 212 84 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2670 3781 1353 Size: 1317 1111 3423 Fragments arranged by size: 3423 1317 1111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2705 4438 4812 4958 387 Size: 1064 138 1116 1733 374 146 1280 Fragments arranged by size: 1733 1280 1116 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2322 2406 2322 Size: 84 5767 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2380 2422 2548 2873 3914 4511 5298 Size: 393 748 42 126 325 1041 597 787 Cuts at: 5298 5566 1239 Size: 268 1524 Fragments arranged by size: 1524 1041 787 748 597 393 325 268 126 42 EcoO109I rG'GnC_Cy Cuts at: 2618 2669 2807 2808 2618 Size: 51 138 1 5661 Fragments arranged by size: 5661 138 51 1 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2395 2515 2994 4998 1678 Size: 717 120 479 2004 2531 Fragments arranged by size: 2531 2004 717 479 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 4492 4524 4566 Size: 32 135 32 481 32 2407 32 42 Cuts at: 4566 4598 1373 Size: 32 2626 Fragments arranged by size: 2626 2407 481 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KasI G'GCGC_C Cuts at: 2990 2990 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 4965 5149 1305 Size: 1128 2532 184 2007 Fragments arranged by size: 2532 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 2501 10 Size: 54 1425 343 234 435 3360 Fragments arranged by size: 3360 1425 435 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2711 2735 2738 2960 3809 4168 4327 Size: 648 1545 24 3 222 849 359 159 Cuts at: 4327 518 Size: 2042 Fragments arranged by size: 2042 1545 849 648 359 222 159 24 3 MspA1I CmG'CkG Cuts at: 1606 2041 2121 2185 2400 2523 2559 2765 2771 Size: 435 80 64 215 123 36 206 6 Cuts at: 2771 2804 2902 3051 3110 3135 3267 3944 5092 Size: 33 98 149 59 25 132 677 1148 Cuts at: 5092 5337 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 435 245 215 206 149 132 123 98 80 64 59 36 33 25 6 NaeI GCC'GGC Cuts at: 2167 2949 2167 Size: 782 5069 NarI GG'CG_CC Cuts at: 2991 2991 Size: 5851 NcoI C'CATG_G Cuts at: 513 1065 2004 2778 513 Size: 552 939 774 3586 Fragments arranged by size: 3586 939 774 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NgoMIV G'CCGG_C Cuts at: 2165 2947 2165 Size: 782 5069 NheI G'CTAG_C Cuts at: 1052 2648 1052 Size: 1596 4255 NotI GC'GGCC_GC Cuts at: 2186 2470 2554 2766 3298 2186 Size: 284 84 212 532 4739 Fragments arranged by size: 4739 532 284 212 84 NspI r_CATG'y Cuts at: 1132 1229 3209 4754 1132 Size: 97 1980 1545 2229 Fragments arranged by size: 2229 1980 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 2062 3025 1108 Size: 954 963 3934 Fragments arranged by size: 3934 963 954 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2669 2807 2904 2669 Size: 138 97 5616 Fragments arranged by size: 5616 138 97 PstI C_TGCA'G Cuts at: 830 3047 3203 830 Size: 2217 156 3478 Fragments arranged by size: 3478 2217 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2121 2400 3051 2121 Size: 279 651 4921 Fragments arranged by size: 4921 651 279 SacI G_AGCT'C Cuts at: 2324 2408 2324 Size: 84 5767 SacII CC_GC'GG Cuts at: 2186 2560 2766 2772 2805 3111 3136 2186 Size: 374 206 6 33 306 25 4901 Fragments arranged by size: 4901 374 306 206 33 25 6 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SexAI A'CCwGG_T Cuts at: 2328 2328 Size: 5851 SfcI C'TryA_G Cuts at: 826 1045 3043 3199 4334 5015 5206 826 Size: 219 1998 156 1135 681 191 1471 Fragments arranged by size: 1998 1471 1135 681 219 191 156 SfoI GGC'GCC Cuts at: 2992 2992 Size: 5851 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 2270 2340 2508 1700 Size: 570 70 168 5043 Fragments arranged by size: 5043 570 168 70 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 3955 4856 5118 820 Size: 197 926 585 1427 901 262 1553 Fragments arranged by size: 1553 1427 926 901 585 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2004 2634 2664 2778 3290 513 Size: 552 939 630 30 114 512 3074 Fragments arranged by size: 3074 939 630 552 512 114 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3028 3878 3895 4048 4233 4678 325 Size: 1255 1448 850 17 153 185 445 1498 Fragments arranged by size: 1498 1448 1255 850 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4097 5809 96 Size: 274 80 33 51 1443 2120 1712 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 2111 1316 Size: 795 5056 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 2867 3980 2867 Size: 1113 4738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2113 3659 276 Size: 53 83 186 936 579 1546 2468 Fragments arranged by size: 2468 1546 936 579 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI