(Linear) (Six-base) MAPSORT of: KIEE4073.seq Check: 1907 from: 1 to: 1146 KIEE4073.orf With 182 enzymes: SgfI * July 3, 2012 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 460 596 1146 Size: 460 136 550 Fragments arranged by size: 550 460 136 AatII G_ACGT'C Cuts at: 0 110 1146 Size: 110 1036 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 375 543 1146 Size: 375 168 603 Fragments arranged by size: 603 375 168 AfeI AGC'GCT Cuts at: 0 388 1146 Size: 388 758 AflIII A'CryG_T Cuts at: 0 839 1146 Size: 839 307 AlwNI CAG_nnn'CTG Cuts at: 0 1046 1146 Size: 1046 100 ApaI G_GGCC'C Cuts at: 0 668 806 903 1146 Size: 668 138 97 243 Fragments arranged by size: 668 243 138 97 AscI GG'CGCG_CC Cuts at: 0 740 816 1025 1146 Size: 740 76 209 121 Fragments arranged by size: 740 209 121 76 AvaI C'yCGr_G Cuts at: 0 919 1120 1146 Size: 919 201 26 Fragments arranged by size: 919 201 26 BanI G'GyrC_C Cuts at: 0 248 985 1115 1146 Size: 248 737 130 31 Fragments arranged by size: 737 248 130 31 BanII G_rGCy'C Cuts at: 0 319 403 668 806 884 903 920 1146 Size: 319 84 265 138 78 19 17 226 Fragments arranged by size: 319 265 226 138 84 78 19 17 BbeI G_GCGC'C Cuts at: 0 989 1146 Size: 989 157 BglI GCCn_nnn'nGGC Cuts at: 0 174 1146 Size: 174 972 Bme1580I G_kGCm'C Cuts at: 0 541 668 806 872 903 1120 1146 Size: 541 127 138 66 31 217 26 Fragments arranged by size: 541 217 138 127 66 31 26 BmrI ACTGGGnnnn_n' Cuts at: 0 70 138 1146 Size: 70 68 1008 Fragments arranged by size: 1008 70 68 BmtI G_CTAG'C Cuts at: 0 647 1146 Size: 647 499 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 417 868 1146 Size: 417 451 278 Fragments arranged by size: 451 417 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 1146 Size: 508 638 BsaI GGTCTCn'nnnn_ Cuts at: 0 29 1146 Size: 29 1117 BsaAI yAC'GTr Cuts at: 0 840 1146 Size: 840 306 BsaBI GATnn'nnATC Cuts at: 0 700 1146 Size: 700 446 BsaHI Gr'CG_yC Cuts at: 0 107 986 1146 Size: 107 879 160 Fragments arranged by size: 879 160 107 BsaWI w'CCGG_w Cuts at: 0 995 1146 Size: 995 151 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 952 1093 1096 1146 Size: 952 141 3 50 Fragments arranged by size: 952 141 50 3 BseYI C'CCAG_C Cuts at: 0 112 156 902 1146 Size: 112 44 746 244 Fragments arranged by size: 746 244 112 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 580 1000 1146 Size: 349 231 420 146 Fragments arranged by size: 420 349 231 146 BsiEI CG_ry'CG Cuts at: 0 178 184 468 552 764 811 1146 Size: 178 6 284 84 212 47 335 Fragments arranged by size: 335 284 212 178 84 47 6 BsiHKAI G_wGCw'C Cuts at: 0 319 403 1146 Size: 319 84 743 Fragments arranged by size: 743 319 84 BsiWI C'GTAC_G Cuts at: 0 836 1146 Size: 836 310 Bsp1286I G_dGCh'C Cuts at: 0 319 403 541 668 806 872 884 903 Size: 319 84 138 127 138 66 12 19 Cuts at: 903 920 1120 1146 Size: 17 200 26 Fragments arranged by size: 319 200 138 138 127 84 66 26 19 17 12 BspEI T'CCGG_A Cuts at: 0 995 1146 Size: 995 151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 460 596 1146 Size: 460 136 550 Fragments arranged by size: 550 460 136 BsrBI CCG'CTC Cuts at: 0 273 1146 Size: 273 873 BsrFI r'CCGG_y Cuts at: 0 160 265 335 503 942 1146 Size: 160 105 70 168 439 204 Fragments arranged by size: 439 204 168 160 105 70 BssHII G'CGCG_C Cuts at: 0 740 816 1025 1146 Size: 740 76 209 121 Fragments arranged by size: 740 209 121 76 BssSI C'ACGA_G Cuts at: 0 67 1146 Size: 67 1079 BstAPI GCAn_nnn'nTGC Cuts at: 0 327 1046 1146 Size: 327 719 100 Fragments arranged by size: 719 327 100 BstXI CCAn_nnnn'nTGG Cuts at: 0 636 708 1146 Size: 636 72 438 Fragments arranged by size: 636 438 72 BstYI r'GATC_y Cuts at: 0 1010 1146 Size: 1010 136 BtgI C'CryG_G Cuts at: 0 178 552 707 758 764 773 797 1103 Size: 178 374 155 51 6 9 24 306 Cuts at: 1103 1128 1146 Size: 25 18 Fragments arranged by size: 374 306 178 155 51 25 24 18 9 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 728 1146 Size: 728 418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 192 227 1146 Size: 192 35 919 Fragments arranged by size: 919 192 35 DraIII CAC_nnn'GTG Cuts at: 0 327 1020 1146 Size: 327 693 126 Fragments arranged by size: 693 327 126 EaeI y'GGCC_r Cuts at: 0 59 175 181 465 494 549 626 761 Size: 59 116 6 284 29 55 77 135 Cuts at: 761 1146 Size: 385 Fragments arranged by size: 385 284 135 116 77 59 55 29 6 EagI C'GGCC_G Cuts at: 0 175 181 465 549 761 1146 Size: 175 6 284 84 212 385 Fragments arranged by size: 385 284 212 175 84 6 EarI CTCTTCn'nnn_ Cuts at: 0 665 1146 Size: 665 481 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 700 1146 Size: 700 446 EcoICRI GAG'CTC Cuts at: 0 317 401 1146 Size: 317 84 745 Fragments arranged by size: 745 317 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 375 417 543 868 1146 Size: 375 42 126 325 278 Fragments arranged by size: 375 325 278 126 42 EcoO109I rG'GnC_Cy Cuts at: 0 613 664 802 803 1146 Size: 613 51 138 1 343 Fragments arranged by size: 613 343 138 51 1 HaeII r_GCGC'y Cuts at: 0 390 510 989 1146 Size: 390 120 479 157 Fragments arranged by size: 479 390 157 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 48 80 1146 Size: 48 32 1066 Fragments arranged by size: 1066 48 32 KasI G'GCGC_C Cuts at: 0 985 1146 Size: 985 161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 428 1146 Size: 428 718 MscI TGG'CCA Cuts at: 0 61 496 1146 Size: 61 435 650 Fragments arranged by size: 650 435 61 MslI CAynn'nnrTG Cuts at: 0 706 730 733 955 1146 Size: 706 24 3 222 191 Fragments arranged by size: 706 222 191 24 3 MspA1I CmG'CkG Cuts at: 0 36 116 180 395 518 554 760 766 Size: 36 80 64 215 123 36 206 6 Cuts at: 766 799 897 1046 1105 1130 1146 Size: 33 98 149 59 25 16 Fragments arranged by size: 215 206 149 123 98 80 64 59 36 36 33 25 16 6 NaeI GCC'GGC Cuts at: 0 162 944 1146 Size: 162 782 202 Fragments arranged by size: 782 202 162 NarI GG'CG_CC Cuts at: 0 986 1146 Size: 986 160 NcoI C'CATG_G Cuts at: 0 773 1146 Size: 773 373 NgoMIV G'CCGG_C Cuts at: 0 160 942 1146 Size: 160 782 204 Fragments arranged by size: 782 204 160 NheI G'CTAG_C Cuts at: 0 643 1146 Size: 643 503 NotI GC'GGCC_GC Cuts at: 0 181 465 549 761 1146 Size: 181 284 84 212 385 Fragments arranged by size: 385 284 212 181 84 PflMI CCAn_nnn'nTGG Cuts at: 0 57 1020 1146 Size: 57 963 126 Fragments arranged by size: 963 126 57 PspOMI G'GGCC_C Cuts at: 0 664 802 899 1146 Size: 664 138 97 247 Fragments arranged by size: 664 247 138 97 PstI C_TGCA'G Cuts at: 0 1042 1146 Size: 1042 104 PvuII CAG'CTG Cuts at: 0 116 395 1046 1146 Size: 116 279 651 100 Fragments arranged by size: 651 279 116 100 SacI G_AGCT'C Cuts at: 0 319 403 1146 Size: 319 84 743 Fragments arranged by size: 743 319 84 SacII CC_GC'GG Cuts at: 0 181 555 761 767 800 1106 1131 1146 Size: 181 374 206 6 33 306 25 15 Fragments arranged by size: 374 306 206 181 33 25 15 6 SexAI A'CCwGG_T Cuts at: 0 323 1146 Size: 323 823 SfcI C'TryA_G Cuts at: 0 1038 1146 Size: 1038 108 SfoI GGC'GCC Cuts at: 0 987 1146 Size: 987 159 SgrAI Cr'CCGG_yG Cuts at: 0 265 335 503 1146 Size: 265 70 168 643 Fragments arranged by size: 643 265 168 70 SmlI C'TyrA_G Cuts at: 0 523 1146 Size: 523 623 StyI C'CwwG_G Cuts at: 0 629 659 773 1146 Size: 629 30 114 373 Fragments arranged by size: 629 373 114 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1023 1146 Size: 1023 123 Tth111I GACn'n_nGTC Cuts at: 0 106 1146 Size: 106 1040 XmnI GAAnn'nnTTC Cuts at: 0 862 1146 Size: 862 284 ZraI GAC'GTC Cuts at: 0 108 1146 Size: 108 1038 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AlwNI ApaI AscI AvaI BanI BanII BbeI BglI Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI PflMI PspOMI PstI PvuII SacI SacII SexAI SfcI SfoI SgrAI SmlI StyI TaqII Tth111I XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BplI Bpu10I BsaXI BsmI BsmBI BspHI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI XhoI XmaI