Restriction map for
pFN21AE3461
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AhdI Alw44I AocI
Aor51HI ApaLI AscI AspEI AviII
AvrII BalI BbeI BbrPI BbvCI
BglII BlnI BsaAI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI MluI MluNI MroI
MscI NarI NheI NotI NruI
NsiI PacI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 2462 GACGTC GACGT^C
Acc65I 289 GGTACC G^GTACC
AflIII 3056 ACATGT A^C[GA][CT]GT
AgeI 1823 ACCGGT A^CCGGT
Asp718I 289 GGTACC G^GTACC
AspI 2260 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1273 GCACACCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3969 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1451 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1451 GCTCAGC GC^T[ACGT]AGC
BsmBI 659 CGTCTC CGTCTC(1/5)
BspHI 3441 TCATGA T^CATGA
BspLU11I 3056 ACATGT A^CATGT
BssSI 3341 CACGAG CACGAG(-5/-1)
BstBI 995 TTCGAA TT^CGAA
CelII 1451 GCTCAGC GC^T[ACGT]AGC
EcoRI 3393 GAATTC G^AATTC
HaeII 3644 AGCGCC [GA]GCGC^[CT]
HincII 519 GTCAAC GT[CT]^[GA]AC
HindII 519 GTCAAC GT[CT]^[GA]AC
KpnI 289 GGTACC GGTAC^C
MaeII 2463 ACGT A^CGT
MfeI 2512 CAATTG C^AATTG
MunI 2512 CAATTG C^AATTG
NaeI 3552 GCCGGC GCC^GGC
NgoMIV 3552 GCCGGC G^CCGGC
NspV 995 TTCGAA TT^CGAA
PflFI 2260 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1823 ACCGGT A^CCGGT
RcaI 3441 TCATGA T^CATGA
SapI 487 GAAGAGC GCTCTTC(1/4)
SfuI 995 TTCGAA TT^CGAA
SpeI 616 ACTAGT A^CTAGT
StuI 1797 AGGCCT AGG^CCT
Tth111I 2260 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
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AccI 1998 GTCTAC GT^[AC][GT]AC
3412 GTAGAC
AcyI 1645 GACGCC G[GA]^CG[CT]C
2462 GACGTC
AflII 1390 CTTAAG C^TTAAG
1513 CTTAAG
ApaI 3907 GGGCCC GGGCC^C
3967 GGGCCC
AseI 979 ATTAAT AT^TAAT
1873 ATTAAT
AsnI 979 ATTAAT AT^TAAT
1873 ATTAAT
Asp700I 63 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2135 GAAGAATTTC
BamHI 120 GGATCC G^GATCC
246 GGATCC
BbsI 1642 GAAGAC GAAGAC(2/6)
2839 GAAGAC
BclI 1917 TGATCA T^GATCA
2892 TGATCA
BfrI 1390 CTTAAG C^TTAAG
1513 CTTAAG
BpuAI 1642 GAAGAC GAAGAC(2/6)
2839 GAAGAC
BsaHI 1645 GACGCC G[GA]^CG[CT]C
2462 GACGTC
BsaI 2827 GAGACC GGTCTC(1/5)
3693 GGTCTC
BsaWI 101 ACCGGA [AT]^CCGG[AT]
1823 ACCGGT
BsiEI 2725 CGGCCG CG[GA][CT]^CG
3550 CGGCCG
BsmI 1668 GAATGC GAATGC(1/-1)
3035 GAATGC
BspMI 568 ACCTGC ACCTGC(4/8)
2602 GCAGGT
BsrBI 1298 CCGCTC CCGCTC(-3/-3)
3564 GAGCGG
BsrDI 2060 GCAATG GCAATG(2/0)
2753 GCAATG
BsrFI 1823 ACCGGT [GA]^CCGG[CT]
3552 GCCGGC
BstAPI 2744 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3978 GCAAGATGTGC
BstEII 90 GGTGACC G^GT[ACGT]ACC
1819 GGTAACC
BstPI 90 GGTGACC G^GT[ACGT]ACC
1819 GGTAACC
BstXI 124 CCAAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2507 CCAGTCAATTGG
Cfr10I 1823 ACCGGT [GA]^CCGG[CT]
3552 GCCGGC
DraIII 1198 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
3574 CACTCGGTG
EagI 2725 CGGCCG C^GGCCG
3550 CGGCCG
EclXI 2725 CGGCCG C^GGCCG
3550 CGGCCG
Eco52I 2725 CGGCCG C^GGCCG
3550 CGGCCG
EcoO65I 90 GGTGACC G^GT[ACGT]ACC
1819 GGTAACC
FbaI 1917 TGATCA T^GATCA
2892 TGATCA
Hin1I 1645 GACGCC G[GA]^CG[CT]C
2462 GACGTC
NcoI 1973 CCATGG C^CATGG
3311 CCATGG
NdeI 1024 CATATG CA^TATG
3352 CATATG
NspI 762 ACATGC [GA]CATG^[CT]
3056 ACATGT
PpuMI 2625 GGGTCCT [GA]G^G[AT]CC[CT]
3941 AGGACCC
PshBI 979 ATTAAT AT^TAAT
1873 ATTAAT
PvuII 527 CAGCTG CAG^CTG
2227 CAGCTG
SacI 3627 GAGCTC GAGCT^C
3844 GAGCTC
SmaI 29 CCCGGG CCC^GGG
1484 CCCGGG
XmaCI 29 CCCGGG C^CCGGG
1484 CCCGGG
XmaI 29 CCCGGG C^CCGGG
1484 CCCGGG
XmnI 63 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2135 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbvI BciVI
BcnI BfaI BmyI BpmI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PflMI PleI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII