################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbsI BciVI BclI BfrI BlnI BpuAI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eco47III EcoRI EcoRV EcoT22I FbaI FspI HpaI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII ScaI SfuI SnaBI SpeI SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflIII 4526 ACGTGT A^C[GA][CT]GT AgeI 4382 ACCGGT A^CCGGT Alw44I 4522 GTGCAC G^TGCAC ApaLI 4522 GTGCAC G^TGCAC AspI 1317 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 4525 CACGTG CAC^GTG BcgI 3240 GCAGGACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 4525 CACGTG [CT]AC^GT[GA] BsaBI 82 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4382 ACCGGT [AT]^CCGG[AT] BsmBI 1166 CGTCTC CGTCTC(1/5) BspHI 102 TCATGA T^CATGA BssSI 702 CACGAG CACGAG(-5/-1) BstEII 354 GGTGACC G^GT[ACGT]ACC BstPI 354 GGTGACC G^GT[ACGT]ACC EcoO65I 354 GGTGACC G^GT[ACGT]ACC HincII 4252 GTCGAC GT[CT]^[GA]AC HindII 4252 GTCGAC GT[CT]^[GA]AC MamI 82 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 178 TTAA T^TAA PflFI 1317 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4382 ACCGGT A^CCGGT PmaCI 4525 CACGTG CAC^GTG PmlI 4525 CACGTG CAC^GTG PshAI 23 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 102 TCATGA T^CATGA SalI 4252 GTCGAC G^TCGAC SexAI 425 ACCTGGT A^CC[AT]GGT SfiI 4227 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 3693 CGCCGGCG C[GA]^CCGG[CT]G StuI 4515 AGGCCT AGG^CCT Tru9I 178 TTAA T^TAA Tth111I 1317 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1461 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 1999 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2725 GACCCCTCGTC AocI 739 CCTGAGG CC^T[ACGT]AGG 2479 CCTCAGG AspEI 1999 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2725 GACCCCTCGTC BglII 1709 AGATCT A^GATCT 1886 AGATCT BsmI 2400 GCATTC GAATGC(1/-1) 2645 GCATTC BssHII 1769 GCGCGC G^CGCGC 2556 GCGCGC Bsu36I 739 CCTGAGG CC^T[ACGT]AGG 2479 CCTCAGG DraIII 349 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 2365 CACACGGTG DrdI 4243 GACCGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4377 GACGCACCGGTC Eam1105I 1999 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2725 GACCCCTCGTC Eco81I 739 CCTGAGG CC^T[ACGT]AGG 2479 CCTCAGG HindIII 198 AAGCTT A^AGCTT 1456 AAGCTT NspI 209 GCATGC [GA]CATG^[CT] 636 GCATGC PpuMI 2483 AGGACCC [GA]G^G[AT]CC[CT] 2935 GGGTCCC SbfI 1530 CCTGCAGG CCTGCA^GG 4440 CCTGCAGG SmaI 3921 CCCGGG CCC^GGG 4238 CCCGGG SphI 209 GCATGC GCATG^C 636 GCATGC Sse8387I 1530 CCTGCAGG CCTGCA^GG 4440 CCTGCAGG XmaCI 3921 CCCGGG C^CCGGG 4238 CCCGGG XmaI 3921 CCCGGG C^CCGGG 4238 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FseI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII