(Linear) (Six-base) MAPSORT of: KIEE3473.seq Check: 5091 from: 1 to: 4611 KIEE3473.orf With 182 enzymes: SgfI * July 3, 2012 17:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2024 4611 Size: 2024 2587 AccI GT'mk_AC Cuts at: 0 56 77 341 4253 4611 Size: 56 21 264 3912 358 Fragments arranged by size: 3912 358 264 56 21 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 778 813 916 1897 3469 3975 4156 Size: 46 732 35 103 981 1572 506 181 Cuts at: 4156 4611 Size: 455 Fragments arranged by size: 1572 981 732 506 455 181 103 46 35 AflIII A'CryG_T Cuts at: 0 4526 4611 Size: 4526 85 AgeI A'CCGG_T Cuts at: 0 4382 4611 Size: 4382 229 AhdI GACnn_n'nnGTC Cuts at: 0 2004 2730 4611 Size: 2004 726 1881 Fragments arranged by size: 2004 1881 726 AleI CACnn'nnGTG Cuts at: 0 2018 4611 Size: 2018 2593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 886 2771 4611 Size: 886 1885 1840 Fragments arranged by size: 1885 1840 886 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 586 618 4611 Size: 586 32 3993 Fragments arranged by size: 3993 586 32 AlwNI CAG_nnn'CTG Cuts at: 0 1485 1494 1539 1575 1604 1731 1875 2214 Size: 1485 9 45 36 29 127 144 339 Cuts at: 2214 3109 3411 3571 4488 4611 Size: 895 302 160 917 123 Fragments arranged by size: 1485 917 895 339 302 160 144 127 123 45 36 29 9 ApaI G_GGCC'C Cuts at: 0 1078 3055 4024 4196 4399 4611 Size: 1078 1977 969 172 203 212 Fragments arranged by size: 1977 1078 969 212 203 172 ApaLI G'TGCA_C Cuts at: 0 4522 4611 Size: 4522 89 AvaI C'yCGr_G Cuts at: 0 364 397 1264 3061 3921 4024 4238 4611 Size: 364 33 867 1797 860 103 214 373 Fragments arranged by size: 1797 867 860 373 364 214 103 33 BanI G'GyrC_C Cuts at: 0 271 862 1025 1235 1431 2029 2241 2348 Size: 271 591 163 210 196 598 212 107 Cuts at: 2348 2370 3925 4611 Size: 22 1555 686 Fragments arranged by size: 1555 686 598 591 271 212 210 196 163 107 22 BanII G_rGCy'C Cuts at: 0 50 389 887 904 1078 1967 2125 2132 Size: 50 339 498 17 174 889 158 7 Cuts at: 2132 2165 2449 2479 2582 3055 3327 3457 3584 Size: 33 284 30 103 473 272 130 127 Cuts at: 3584 3845 4024 4045 4084 4196 4399 4611 Size: 261 179 21 39 112 203 212 Fragments arranged by size: 889 498 473 339 284 272 261 212 203 179 174 158 130 127 112 103 50 39 33 30 21 17 7 BbeI G_GCGC'C Cuts at: 0 1029 1239 1435 2033 4611 Size: 1029 210 196 598 2578 Fragments arranged by size: 2578 1029 598 210 196 BbvCI CC'TCA_GC Cuts at: 0 1127 1597 2341 3799 4315 4611 Size: 1127 470 744 1458 516 296 Fragments arranged by size: 1458 1127 744 516 470 296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3229 3263 4611 Size: 3229 34 1348 Fragments arranged by size: 3229 1348 34 BglI GCCn_nnn'nGGC Cuts at: 0 1082 2264 2379 2686 2884 3766 3862 4234 Size: 1082 1182 115 307 198 882 96 372 Cuts at: 4234 4339 4611 Size: 105 272 Fragments arranged by size: 1182 1082 882 372 307 272 198 115 105 96 BglII A'GATC_T Cuts at: 0 1709 1886 4611 Size: 1709 177 2725 Fragments arranged by size: 2725 1709 177 BlpI GC'TnA_GC Cuts at: 0 44 1586 2176 2411 2473 2611 4051 4216 Size: 44 1542 590 235 62 138 1440 165 Cuts at: 4216 4611 Size: 395 Fragments arranged by size: 1542 1440 590 395 235 165 138 62 44 Bme1580I G_kGCm'C Cuts at: 0 274 1078 2246 2375 3055 3467 3534 3928 Size: 274 804 1168 129 680 412 67 394 Cuts at: 3928 4024 4196 4399 4526 4611 Size: 96 172 203 127 85 Fragments arranged by size: 1168 804 680 412 394 274 203 172 129 127 96 85 67 BmgBI CAC'GTC Cuts at: 0 2262 4611 Size: 2262 2349 BmrI ACTGGGnnnn_n' Cuts at: 0 1509 1839 2202 2696 4611 Size: 1509 330 363 494 1915 Fragments arranged by size: 1915 1509 494 363 330 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 408 3585 3861 4611 Size: 408 3177 276 750 Fragments arranged by size: 3177 750 408 276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 496 1774 1867 1933 2068 2095 2299 Size: 34 462 1278 93 66 135 27 204 Cuts at: 2299 2543 2889 2894 3006 3165 3433 3856 4611 Size: 244 346 5 112 159 268 423 755 Fragments arranged by size: 1278 755 462 423 346 268 244 204 159 135 112 93 66 34 27 5 Bpu10I CC'TnA_GC Cuts at: 0 1127 1597 2341 2353 3799 4039 4102 4315 Size: 1127 470 744 12 1446 240 63 213 Cuts at: 4315 4611 Size: 296 Fragments arranged by size: 1446 1127 744 470 296 240 213 63 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 870 1783 1960 2130 3315 3481 4243 Size: 136 734 913 177 170 1185 166 762 Cuts at: 4243 4611 Size: 368 Fragments arranged by size: 1185 913 762 734 368 177 170 166 136 BsaI GGTCTCn'nnnn_ Cuts at: 0 109 406 580 3071 3371 3433 4611 Size: 109 297 174 2491 300 62 1178 Fragments arranged by size: 2491 1178 300 297 174 109 62 BsaAI yAC'GTr Cuts at: 0 4527 4611 Size: 4527 84 BsaBI GATnn'nnATC Cuts at: 0 86 4611 Size: 86 4525 BsaHI Gr'CG_yC Cuts at: 0 551 1026 1106 1236 1432 2000 2030 2057 Size: 551 475 80 130 196 568 30 27 Cuts at: 2057 3286 4088 4342 4363 4611 Size: 1229 802 254 21 248 Fragments arranged by size: 1229 802 568 551 475 254 248 196 130 80 30 27 21 BsaWI w'CCGG_w Cuts at: 0 4382 4611 Size: 4382 229 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1840 1870 2272 2302 3001 3031 3070 3100 Size: 1840 30 402 30 699 30 39 30 Cuts at: 3100 3367 3397 4441 4471 4611 Size: 267 30 1044 30 140 Fragments arranged by size: 1840 1044 699 402 267 140 39 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1329 1585 1636 1828 2706 3009 3141 3574 Size: 1329 256 51 192 878 303 132 433 Cuts at: 3574 3853 4303 4461 4611 Size: 279 450 158 150 Fragments arranged by size: 1329 878 450 433 303 279 256 192 158 150 132 51 BseYI C'CCAG_C Cuts at: 0 1542 1568 1659 2449 2671 2773 3289 3583 Size: 1542 26 91 790 222 102 516 294 Cuts at: 3583 4129 4611 Size: 546 482 Fragments arranged by size: 1542 790 546 516 482 294 222 102 91 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 925 1277 1291 1420 1996 2053 2070 2641 Size: 925 352 14 129 576 57 17 571 Cuts at: 2641 3930 4611 Size: 1289 681 Fragments arranged by size: 1289 925 681 576 571 352 129 57 17 14 BsiEI CG_ry'CG Cuts at: 0 304 954 2268 2272 3261 4034 4295 4508 Size: 304 650 1314 4 989 773 261 213 Cuts at: 4508 4611 Size: 103 Fragments arranged by size: 1314 989 773 650 304 261 213 103 4 BsiHKAI G_wGCw'C Cuts at: 0 512 887 1967 2132 2165 2417 2582 2698 Size: 512 375 1080 165 33 252 165 116 Cuts at: 2698 3457 3497 3594 4045 4526 4611 Size: 759 40 97 451 481 85 Fragments arranged by size: 1080 759 512 481 451 375 252 165 165 116 97 85 40 33 BsmI GAATG_Cn' Cuts at: 0 2400 2645 4611 Size: 2400 245 1966 Fragments arranged by size: 2400 1966 245 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1172 4611 Size: 1172 3439 Bsp1286I G_dGCh'C Cuts at: 0 50 274 389 512 887 904 1078 1967 Size: 50 224 115 123 375 17 174 889 Cuts at: 1967 2125 2132 2165 2246 2375 2417 2449 2479 Size: 158 7 33 81 129 42 32 30 Cuts at: 2479 2582 2698 3055 3327 3457 3467 3497 3534 Size: 103 116 357 272 130 10 30 37 Cuts at: 3534 3584 3594 3845 3928 4024 4045 4084 4196 Size: 50 10 251 83 96 21 39 112 Cuts at: 4196 4399 4526 4611 Size: 203 127 85 Fragments arranged by size: 889 375 357 272 251 224 203 174 158 130 129 127 123 116 115 112 103 96 85 83 81 50 50 42 39 37 33 32 30 30 21 17 10 10 7 BspHI T'CATG_A Cuts at: 0 102 4611 Size: 102 4509 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 327 947 1493 2024 2794 3089 4611 Size: 327 620 546 531 770 295 1522 Fragments arranged by size: 1522 770 620 546 531 327 295 BsrBI CCG'CTC Cuts at: 0 491 765 1269 2610 3507 3868 4220 4611 Size: 491 274 504 1341 897 361 352 391 Fragments arranged by size: 1341 897 504 491 391 361 352 274 BsrFI r'CCGG_y Cuts at: 0 72 1093 2063 2180 2267 2382 2883 3260 Size: 72 1021 970 117 87 115 501 377 Cuts at: 3260 3694 4033 4382 4465 4503 4611 Size: 434 339 349 83 38 108 Fragments arranged by size: 1021 970 501 434 377 349 339 117 115 108 87 83 72 38 BssHII G'CGCG_C Cuts at: 0 1769 2556 4611 Size: 1769 787 2055 Fragments arranged by size: 2055 1769 787 BssSI C'ACGA_G Cuts at: 0 702 4611 Size: 702 3909 BstAPI GCAn_nnn'nTGC Cuts at: 0 1300 1539 2964 4611 Size: 1300 239 1425 1647 Fragments arranged by size: 1647 1425 1300 239 BstEII G'GTnAC_C Cuts at: 0 354 4611 Size: 354 4257 BstXI CCAn_nnnn'nTGG Cuts at: 0 2152 2773 3526 4611 Size: 2152 621 753 1085 Fragments arranged by size: 2152 1085 753 621 BstYI r'GATC_y Cuts at: 0 147 1360 1475 1709 1886 3105 4611 Size: 147 1213 115 234 177 1219 1506 Fragments arranged by size: 1506 1219 1213 234 177 147 115 Bsu36I CC'TnA_GG Cuts at: 0 740 2480 4611 Size: 740 1740 2131 Fragments arranged by size: 2131 1740 740 BtgI C'CryG_G Cuts at: 0 35 61 590 1112 1776 2488 2766 2930 Size: 35 26 529 522 664 712 278 164 Cuts at: 2930 3213 3234 3386 3968 4152 4196 4460 4565 Size: 283 21 152 582 184 44 264 105 Cuts at: 4565 4611 Size: 46 Fragments arranged by size: 712 664 582 529 522 283 278 264 184 164 152 105 46 44 35 26 21 BtsI GCAGTG_nn' Cuts at: 0 1253 1602 1905 2541 3168 3594 4210 4611 Size: 1253 349 303 636 627 426 616 401 Fragments arranged by size: 1253 636 627 616 426 401 349 303 DraIII CAC_nnn'GTG Cuts at: 0 354 2370 4611 Size: 354 2016 2241 Fragments arranged by size: 2241 2016 354 DrdI GACnn_nn'nnGTC Cuts at: 0 4249 4383 4611 Size: 4249 134 228 Fragments arranged by size: 4249 228 134 EaeI y'GGCC_r Cuts at: 0 64 301 572 1058 1091 1221 1779 2142 Size: 64 237 271 486 33 130 558 363 Cuts at: 2142 2265 2269 2327 2654 2828 2885 3258 3758 Size: 123 4 58 327 174 57 373 500 Cuts at: 3758 3863 3965 4031 4266 4292 4463 4505 4611 Size: 105 102 66 235 26 171 42 106 Fragments arranged by size: 558 500 486 373 363 327 271 237 235 174 171 130 123 106 105 102 66 64 58 57 42 33 26 4 EagI C'GGCC_G Cuts at: 0 301 2265 2269 3258 4031 4292 4505 4611 Size: 301 1964 4 989 773 261 213 106 Fragments arranged by size: 1964 989 773 301 261 213 106 4 EarI CTCTTCn'nnn_ Cuts at: 0 671 1127 1873 1939 2524 3445 4013 4282 Size: 671 456 746 66 585 921 568 269 Cuts at: 4282 4611 Size: 329 Fragments arranged by size: 921 746 671 585 568 456 329 269 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1704 3279 3783 3784 4359 4611 Size: 1704 1575 504 1 575 252 Fragments arranged by size: 1704 1575 575 504 252 1 EcoICRI GAG'CTC Cuts at: 0 885 1965 2130 2163 2580 3455 4043 4611 Size: 885 1080 165 33 417 875 588 568 Fragments arranged by size: 1080 885 875 588 568 417 165 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 46 496 778 813 916 1774 1867 Size: 34 12 450 282 35 103 858 93 Cuts at: 1867 1897 1933 2068 2095 2299 2543 2889 2894 Size: 30 36 135 27 204 244 346 5 Cuts at: 2894 3006 3165 3433 3469 3856 3975 4156 4611 Size: 112 159 268 36 387 119 181 455 Fragments arranged by size: 858 455 450 387 346 282 268 244 204 181 159 135 119 112 103 93 36 36 35 34 30 27 12 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 334 2230 2356 4611 Size: 334 1896 126 2255 Fragments arranged by size: 2255 1896 334 126 EcoO109I rG'GnC_Cy Cuts at: 0 1074 1872 2484 2507 2544 2840 2936 3051 Size: 1074 798 612 23 37 296 96 115 Cuts at: 3051 3960 4020 4021 4396 4611 Size: 909 60 1 375 215 Fragments arranged by size: 1074 909 798 612 375 296 215 115 96 60 37 23 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3747 4611 Size: 3747 864 FseI GG_CCGG'CC Cuts at: 0 1097 2271 4037 4611 Size: 1097 1174 1766 574 Fragments arranged by size: 1766 1174 1097 574 HaeII r_GCGC'y Cuts at: 0 1029 1239 1435 1675 2033 3212 3321 4611 Size: 1029 210 196 240 358 1179 109 1290 Fragments arranged by size: 1290 1179 1029 358 240 210 196 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 655 687 1308 1340 1990 2022 2272 2304 Size: 655 32 621 32 650 32 250 32 Cuts at: 2304 2716 2748 2887 2919 3070 3102 4611 Size: 412 32 139 32 151 32 1509 Fragments arranged by size: 1509 655 650 621 412 250 151 139 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 4254 4611 Size: 4254 357 HindIII A'AGCT_T Cuts at: 0 198 1456 4611 Size: 198 1258 3155 Fragments arranged by size: 3155 1258 198 KasI G'GCGC_C Cuts at: 0 1025 1235 1431 2029 4611 Size: 1025 210 196 598 2582 Fragments arranged by size: 2582 1025 598 210 196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1724 3770 4581 4611 Size: 1724 2046 811 30 Fragments arranged by size: 2046 1724 811 30 MscI TGG'CCA Cuts at: 0 2656 2830 3760 4268 4611 Size: 2656 174 930 508 343 Fragments arranged by size: 2656 930 508 343 174 MslI CAynn'nnrTG Cuts at: 0 92 545 868 874 2018 4611 Size: 92 453 323 6 1144 2593 Fragments arranged by size: 2593 1144 453 323 92 6 MspA1I CmG'CkG Cuts at: 0 69 261 756 1404 1422 1731 1752 1778 Size: 69 192 495 648 18 309 21 26 Cuts at: 1778 1794 1824 1953 2490 2573 2837 3780 3970 Size: 16 30 129 537 83 264 943 190 Cuts at: 3970 4050 4116 4154 4185 4319 4537 4611 Size: 80 66 38 31 134 218 74 Fragments arranged by size: 943 648 537 495 309 264 218 192 190 134 129 83 80 74 69 66 38 31 30 26 21 18 16 NaeI GCC'GGC Cuts at: 0 1095 2065 2182 2269 2384 2885 3262 3696 Size: 1095 970 117 87 115 501 377 434 Cuts at: 3696 4035 4467 4505 4611 Size: 339 432 38 106 Fragments arranged by size: 1095 970 501 434 432 377 339 117 115 106 87 38 NarI GG'CG_CC Cuts at: 0 1026 1236 1432 2030 4611 Size: 1026 210 196 598 2581 Fragments arranged by size: 2581 1026 598 210 196 NcoI C'CATG_G Cuts at: 0 590 1112 3386 4565 4611 Size: 590 522 2274 1179 46 Fragments arranged by size: 2274 1179 590 522 46 NgoMIV G'CCGG_C Cuts at: 0 1093 2063 2180 2267 2382 2883 3260 3694 Size: 1093 970 117 87 115 501 377 434 Cuts at: 3694 4033 4465 4503 4611 Size: 339 432 38 108 Fragments arranged by size: 1093 970 501 434 432 377 339 117 115 108 87 38 NspI r_CATG'y Cuts at: 0 213 640 4611 Size: 213 427 3971 Fragments arranged by size: 3971 427 213 PasI CC'CwG_GG Cuts at: 0 2034 2503 3048 4611 Size: 2034 469 545 1563 Fragments arranged by size: 2034 1563 545 469 PflMI CCAn_nnn'nTGG Cuts at: 0 125 1575 2214 2238 3411 4611 Size: 125 1450 639 24 1173 1200 Fragments arranged by size: 1450 1200 1173 639 125 24 PfoI T'CCnGG_A Cuts at: 0 143 4302 4611 Size: 143 4159 309 Fragments arranged by size: 4159 309 143 PmlI CAC'GTG Cuts at: 0 4527 4611 Size: 4527 84 PpuMI rG'GwC_Cy Cuts at: 0 2484 2936 4611 Size: 2484 452 1675 Fragments arranged by size: 2484 1675 452 PshAI GACnn'nnGTC Cuts at: 0 27 4611 Size: 27 4584 PspOMI G'GGCC_C Cuts at: 0 1074 3051 4020 4192 4395 4611 Size: 1074 1977 969 172 203 216 Fragments arranged by size: 1977 1074 969 216 203 172 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1474 1506 4611 Size: 1474 32 3105 Fragments arranged by size: 3105 1474 32 PstI C_TGCA'G Cuts at: 0 335 1454 1490 1535 1823 1922 1973 2717 Size: 335 1119 36 45 288 99 51 744 Cuts at: 2717 3785 4445 4611 Size: 1068 660 166 Fragments arranged by size: 1119 1068 744 660 335 288 166 99 51 45 36 PvuII CAG'CTG Cuts at: 0 261 756 1731 1752 1794 1824 4116 4611 Size: 261 495 975 21 42 30 2292 495 Fragments arranged by size: 2292 975 495 495 261 42 30 21 SacI G_AGCT'C Cuts at: 0 887 1967 2132 2165 2582 3457 4045 4611 Size: 887 1080 165 33 417 875 588 566 Fragments arranged by size: 1080 887 875 588 566 417 165 33 SacII CC_GC'GG Cuts at: 0 1779 2491 3971 4155 4611 Size: 1779 712 1480 184 456 Fragments arranged by size: 1779 1480 712 456 184 SalI G'TCGA_C Cuts at: 0 4252 4611 Size: 4252 359 SanDI GG'GwC_CC Cuts at: 0 2936 4611 Size: 2936 1675 SapI GCTCTTCn'nnn_ Cuts at: 0 1873 1939 2524 3445 4611 Size: 1873 66 585 921 1166 Fragments arranged by size: 1873 1166 921 585 66 SbfI CC_TGCA'GG Cuts at: 0 1535 4445 4611 Size: 1535 2910 166 Fragments arranged by size: 2910 1535 166 SexAI A'CCwGG_T Cuts at: 0 425 4611 Size: 425 4186 SfcI C'TryA_G Cuts at: 0 331 1356 1450 1486 1531 1819 1918 1969 Size: 331 1025 94 36 45 288 99 51 Cuts at: 1969 2713 3781 4441 4480 4611 Size: 744 1068 660 39 131 Fragments arranged by size: 1068 1025 744 660 331 288 131 99 94 51 45 39 36 SfiI GGCCn_nnn'nGGCC Cuts at: 0 4234 4611 Size: 4234 377 SfoI GGC'GCC Cuts at: 0 1027 1237 1433 2031 4611 Size: 1027 210 196 598 2580 Fragments arranged by size: 2580 1027 598 210 196 SgrAI Cr'CCGG_yG Cuts at: 0 3694 4611 Size: 3694 917 SmaI CCC'GGG Cuts at: 0 3923 4240 4611 Size: 3923 317 371 Fragments arranged by size: 3923 371 317 SmlI C'TyrA_G Cuts at: 0 151 849 1798 1975 2109 3294 3496 4258 Size: 151 698 949 177 134 1185 202 762 Cuts at: 4258 4611 Size: 353 Fragments arranged by size: 1185 949 762 698 353 202 177 151 134 SphI G_CATG'C Cuts at: 0 213 640 4611 Size: 213 427 3971 Fragments arranged by size: 3971 427 213 SrfI GCCC'GGGC Cuts at: 0 3923 4611 Size: 3923 688 StuI AGG'CCT Cuts at: 0 4517 4611 Size: 4517 94 StyI C'CwwG_G Cuts at: 0 590 1112 2420 2843 2891 3386 3650 4232 Size: 590 522 1308 423 48 495 264 582 Cuts at: 4232 4565 4611 Size: 333 46 Fragments arranged by size: 1308 590 582 522 495 423 333 264 48 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 373 475 1567 1651 4611 Size: 373 102 1092 84 2960 Fragments arranged by size: 2960 1092 373 102 84 TatI w'GTAC_w Cuts at: 0 230 1517 4611 Size: 230 1287 3094 Fragments arranged by size: 3094 1287 230 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2688 2720 3001 3033 4516 4548 4611 Size: 2688 32 281 32 1483 32 63 Fragments arranged by size: 2688 1483 281 63 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1320 4611 Size: 1320 3291 XbaI T'CTAG_A Cuts at: 0 1461 4611 Size: 1461 3150 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 873 1422 1842 4611 Size: 873 549 420 2769 Fragments arranged by size: 2769 873 549 420 XmaI C'CCGG_G Cuts at: 0 3921 4238 4611 Size: 3921 317 373 Fragments arranged by size: 3921 373 317 Enzymes that do cut: AarI AccI AcuI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BmtI BsiWI BspEI BsrDI BsrGI BstBI BstZ17I BtgZI ClaI CspCI DraI EcoRI EcoRV FspI FspAI HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PvuI RsrII ScaI SgfI SnaBI SpeI SspI SwaI XhoI XmnI ZraI