################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssKI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstZ17I CelII CfoI Cfr13I CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI MspA1I MvaI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 35 CCGC CCGC(-3/-1) AflII 2242 CTTAAG C^TTAAG AflIII 1181 ACACGT A^C[GA][CT]GT AgeI 1981 ACCGGT A^CCGGT AocI 650 CCTCAGG CC^T[ACGT]AGG BalI 1310 TGGCCA TGG^CCA BanI 1630 GGTGCC G^G[CT][GA]CC BfrI 2242 CTTAAG C^TTAAG BglI 537 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 1631 GTGCCC G[AGT]GC[ACT]^C BsaI 1580 GAGACC GGTCTC(1/5) BsgI 613 GTGCAG GTGCAG(16/14) Bsp1286I 1631 GTGCCC G[AGT]GC[ACT]^C BspDI 1018 ATCGAT AT^CGAT BspMI 2247 GCAGGT ACCTGC(4/8) BsrDI 599 CATTGC GCAATG(2/0) BsrFI 1981 ACCGGT [GA]^CCGG[CT] BssSI 2043 CACGAG CACGAG(-5/-1) BstF5I 111 GGATG GGATG(2/0) BstXI 862 CCAGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1595 AGATCC [GA]^GATC[CT] Bsu36I 650 CCTCAGG CC^T[ACGT]AGG Cfr10I 1981 ACCGGT [GA]^CCGG[CT] ClaI 1018 ATCGAT AT^CGAT DrdI 516 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1307 CCATGG C^C[GA][CT]GG EaeI 1310 TGGCCA [CT]^GGCC[GA] Eco81I 650 CCTCAGG CC^T[ACGT]AGG EcoNI 1725 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 34 CCCGC CCCGC(4/6) FokI 111 GGATG GGATG(9/13) MaeII 1183 ACGT A^CGT MflI 1595 AGATCC [GA]^GATC[CT] MluNI 1310 TGGCCA TGG^CCA MscI 1310 TGGCCA TGG^CCA MslI 1562 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1307 CCATGG C^CATGG NdeI 1372 CATATG CA^TATG NspI 80 GCATGT [GA]CATG^[CT] PinAI 1981 ACCGGT A^CCGGT SapI 257 GCTCTTC GCTCTTC(1/4) SpeI 171 ACTAGT A^CTAGT SspI 1237 AATATT AAT^ATT StuI 665 AGGCCT AGG^CCT XcmI 1767 CCATTTGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1595 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 62 CGCG CG^CG 1034 CGCG AlwNI 512 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 2248 CAGGTGCTG Asp700I 377 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2212 GAAACCATTC BsaJI 1307 CCATGG C^C[ACGT][ACGT]GG 1635 CCAAGG BsaWI 1981 ACCGGT [AT]^CCGG[AT] 2199 ACCGGA BsmFI 1030 GGGAC GGGAC(10/14) 1111 GGGAC BsmI 1941 GAATGC GAATGC(1/-1) 846 GCATTC BstUI 62 CGCG CG^CG 1034 CGCG Cac8I 297 GCCAGC GC[ACGT]^[ACGT]GC 1299 GCTTGC DraI 951 TTTAAA TTT^AAA 1708 TTTAAA EcoT14I 1307 CCATGG C^C[AT][AT]GG 1635 CCAAGG HapII 1982 CCGG C^CGG 2200 CCGG HgaI 1032 GACGC GACGC(5/10) 592 GCGTC HpaII 1982 CCGG C^CGG 2200 CCGG MfeI 280 CAATTG C^AATTG 829 CAATTG MspI 1982 CCGG C^CGG 2200 CCGG MunI 280 CAATTG C^AATTG 829 CAATTG MvnI 62 CGCG CG^CG 1034 CGCG NlaIV 1630 GGTGCC GG[ACGT]^[ACGT]CC 1984 GGTTCC SfcI 818 CTACAG C^T[GA][CT]AG 889 CTACAG StyI 1307 CCATGG C^C[AT][AT]GG 1635 CCAAGG XmnI 377 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2212 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BpmI BpuAI BsiYI BslI BsmAI BsrI DdeI DpnII EarI EcoRI Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII PleI RsaI Sau3AI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I