(Circular) (Six-base) MAPSORT of: pFN21AE2090.seq Check: 1846 from: 1 to: 7012 pFN21AE2090 7012 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4243 4243 Size: 7012 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4822 278 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4355 1937 Size: 2418 4594 Acc65I G'GTAC_C Cuts at: 4333 4333 Size: 7012 AclI AA'CG_TT Cuts at: 1563 5139 5512 1563 Size: 3576 373 3063 Fragments arranged by size: 3576 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2172 2598 3716 4005 5075 6459 6727 1632 Size: 540 426 1118 289 1070 1384 268 1917 Fragments arranged by size: 1917 1384 1118 1070 540 426 289 268 AflII C'TTAA_G Cuts at: 820 1017 4247 820 Size: 197 3230 3585 Fragments arranged by size: 3585 3230 197 AflIII A'CryG_T Cuts at: 1203 3186 5911 6888 1203 Size: 1983 2725 977 1327 Fragments arranged by size: 2725 1983 1327 977 AgeI A'CCGG_T Cuts at: 3986 5843 3986 Size: 1857 5155 AhdI GACnn_n'nnGTC Cuts at: 5741 5741 Size: 7012 AleI CACnn'nnGTG Cuts at: 1166 3571 1166 Size: 2405 4607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2469 1184 Size: 1285 5727 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2214 2246 3585 3617 2214 Size: 32 1339 32 5609 Fragments arranged by size: 5609 1339 32 32 AlwNI CAG_nnn'CTG Cuts at: 2522 4258 4755 6327 2522 Size: 1736 497 1572 3207 Fragments arranged by size: 3207 1736 1572 497 ApaLI G'TGCA_C Cuts at: 5069 6225 5069 Size: 1156 5856 ApoI r'AATT_y Cuts at: 1493 2050 2383 2805 2980 3151 3710 3762 3877 Size: 557 333 422 175 171 559 52 115 Cuts at: 3877 4129 4321 4547 4699 6808 1493 Size: 252 192 226 152 2109 1697 Fragments arranged by size: 2109 1697 559 557 422 333 252 226 192 175 171 152 115 52 AseI AT'TA_AT Cuts at: 160 1032 5566 160 Size: 872 4534 1606 Fragments arranged by size: 4534 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 AvaI C'yCGr_G Cuts at: 1870 1943 4337 1870 Size: 73 2394 4545 Fragments arranged by size: 4545 2394 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3159 3192 3159 Size: 33 6979 BamHI G'GATC_C Cuts at: 4342 4709 4342 Size: 367 6645 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3635 4333 5788 618 Size: 325 217 627 1848 698 1455 1842 Fragments arranged by size: 1848 1842 1455 698 627 325 217 BanII G_rGCy'C Cuts at: 1391 4331 1391 Size: 2940 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 2148 2504 2922 4249 928 Size: 971 121 128 356 418 1327 3691 Fragments arranged by size: 3691 1327 971 418 356 128 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5203 5237 1903 Size: 34 3266 34 3678 Fragments arranged by size: 3678 3266 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4904 6114 4904 Size: 1210 5802 BclI T'GATC_A Cuts at: 1548 1548 Size: 7012 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2548 5623 136 Size: 107 122 71 631 605 876 3075 1525 Fragments arranged by size: 3075 1525 876 631 605 122 107 71 BglII A'GATC_T Cuts at: 7007 7007 Size: 7012 BlpI GC'TnA_GC Cuts at: 4429 4429 Size: 7012 Bme1580I G_kGCm'C Cuts at: 1790 3640 5073 6229 1790 Size: 1850 1433 1156 2573 Fragments arranged by size: 2573 1850 1433 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7012 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4187 4743 5701 Size: 416 53 83 399 24 2770 556 958 Cuts at: 5701 442 Size: 1753 Fragments arranged by size: 2770 1753 958 556 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7012 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2517 2850 3156 3500 3804 5672 1239 Size: 1278 333 306 344 304 1868 2579 Fragments arranged by size: 2579 1868 1278 344 333 306 304 Bpu10I CC'TnA_GC Cuts at: 4001 4001 Size: 7012 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2841 3651 5137 6002 6300 2841 Size: 810 1486 865 298 3553 Fragments arranged by size: 3553 1486 865 810 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3579 5675 882 Size: 617 2080 2096 2219 Fragments arranged by size: 2219 2096 2080 617 BsaAI yAC'GTr Cuts at: 493 1204 4803 493 Size: 711 3599 2702 Fragments arranged by size: 3599 2702 711 BsaBI GATnn'nnATC Cuts at: 7006 7006 Size: 7012 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4819 5201 5836 275 Size: 53 83 186 936 3286 382 635 1451 Fragments arranged by size: 3286 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3986 4204 5445 5843 6117 6264 6675 3986 Size: 218 1241 398 274 147 411 4323 Fragments arranged by size: 4323 1241 411 398 274 218 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6787 Fragments arranged by size: 6787 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7012 BseYI C'CCAG_C Cuts at: 3300 6215 6711 3300 Size: 2915 496 3601 Fragments arranged by size: 3601 2915 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 1163 Size: 573 903 5536 Fragments arranged by size: 5536 903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4462 5223 6251 1540 Size: 275 186 2461 761 1028 2301 Fragments arranged by size: 2461 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5073 5158 6229 5073 Size: 85 1071 5856 Fragments arranged by size: 5856 1071 85 BsmI GAATG_Cn' Cuts at: 2851 3952 4523 4616 2851 Size: 1101 571 93 5247 Fragments arranged by size: 5247 1101 571 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3640 4331 5073 5158 6229 1391 Size: 399 1850 691 742 85 1071 2174 Fragments arranged by size: 2174 1850 1071 742 691 399 85 BspEI T'CCGG_A Cuts at: 6675 6675 Size: 7012 BspHI T'CATG_A Cuts at: 4901 6631 4901 Size: 1730 5282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4243 4367 844 Size: 3399 124 3489 Fragments arranged by size: 3489 3399 124 BsrBI CCG'CTC Cuts at: 1394 1823 4899 1394 Size: 429 3076 3507 Fragments arranged by size: 3507 3076 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2603 5507 5681 65 Size: 1183 1355 2904 174 1396 Fragments arranged by size: 2904 1396 1355 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3986 5656 5843 1700 Size: 110 2176 1670 187 2869 Fragments arranged by size: 2869 2176 1670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7012 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7012 BssSI C'ACGA_G Cuts at: 4048 5072 6084 4048 Size: 1024 1012 4976 Fragments arranged by size: 4976 1024 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7012 BstBI TT'CG_AA Cuts at: 5816 5816 Size: 7012 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7012 BstXI CCAn_nnnn'nTGG Cuts at: 2874 2874 Size: 7012 BstYI r'GATC_y Cuts at: 1972 3600 4342 4709 5094 5111 6563 6649 6661 Size: 1628 742 367 385 17 1452 86 12 Cuts at: 6661 7007 1972 Size: 346 1977 Fragments arranged by size: 1977 1628 1452 742 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2656 2656 Size: 7012 BtgI C'CryG_G Cuts at: 513 1065 3312 513 Size: 552 2247 4213 Fragments arranged by size: 4213 2247 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6273 Fragments arranged by size: 6273 683 56 BtsI GCAGTG_nn' Cuts at: 4528 5322 5342 4528 Size: 794 20 6198 Fragments arranged by size: 6198 794 20 ClaI AT'CG_AT Cuts at: 1554 3024 1554 Size: 1470 5542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2922 2957 529 Size: 35 2358 35 4584 Fragments arranged by size: 4584 2358 35 35 DraI TTT'AAA Cuts at: 2958 3715 4316 2958 Size: 757 601 5654 Fragments arranged by size: 5654 757 601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2527 6019 809 Size: 507 1211 3492 1802 Fragments arranged by size: 3492 1802 1211 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3315 4459 5348 Size: 54 1425 50 275 18 1485 1144 889 Cuts at: 5348 6977 8 Size: 1629 43 Fragments arranged by size: 1629 1485 1425 1144 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4459 1537 Size: 275 2647 4090 Fragments arranged by size: 4090 2647 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2269 3692 3926 4942 1353 Size: 916 1423 234 1016 3423 Fragments arranged by size: 3423 1423 1016 916 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5599 5973 6119 387 Size: 1064 138 4010 374 146 1280 Fragments arranged by size: 4010 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2517 2598 2850 3156 3500 3716 Size: 393 540 345 81 252 306 344 216 Cuts at: 3716 3804 4005 5075 5672 6459 6727 1239 Size: 88 201 1070 597 787 268 1524 Fragments arranged by size: 1524 1070 787 597 540 393 345 344 306 268 252 216 201 88 81 EcoNI CCTnn'n_nnAGG Cuts at: 3734 3734 Size: 7012 EcoRI G'AATT_C Cuts at: 3151 3762 4129 4321 4699 3151 Size: 611 367 192 378 5464 Fragments arranged by size: 5464 611 378 367 192 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3282 4295 3282 Size: 1013 5999 FspI TGC'GCA Cuts at: 5518 5518 Size: 7012 HaeII r_GCGC'y Cuts at: 1678 6159 1678 Size: 4481 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3193 3225 3269 3301 3338 Size: 32 135 32 1621 32 44 32 37 Cuts at: 3338 3370 3602 3634 5653 5685 5727 5759 1373 Size: 32 232 32 2019 32 42 32 2626 Fragments arranged by size: 2626 2019 1621 232 135 44 42 37 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4356 4602 669 Size: 988 281 2418 246 3079 Fragments arranged by size: 3079 2418 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7012 HpaI GTT'AAC Cuts at: 4602 4602 Size: 7012 KpnI G_GTAC'C Cuts at: 4337 4337 Size: 7012 MfeI C'AATT_G Cuts at: 2285 2834 4611 2285 Size: 549 1777 4686 Fragments arranged by size: 4686 1777 549 MluI A'CGCG_T Cuts at: 6888 6888 Size: 7012 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3424 6126 6310 1305 Size: 2119 2702 184 2007 Fragments arranged by size: 2702 2119 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3317 10 Size: 54 1425 343 1485 3705 Fragments arranged by size: 3705 1485 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3571 4970 5329 5488 518 Size: 648 2405 1399 359 159 2042 Fragments arranged by size: 2405 2042 1399 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4428 5105 6253 6498 1606 Size: 2822 677 1148 245 2120 Fragments arranged by size: 2822 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3312 513 Size: 552 2247 4213 Fragments arranged by size: 4213 2247 552 NdeI CA'TA_TG Cuts at: 387 3378 387 Size: 2991 4021 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7012 NotI GC'GGCC_GC Cuts at: 4459 4459 Size: 7012 NspI r_CATG'y Cuts at: 1132 1229 2089 4370 5915 1132 Size: 97 860 2281 1545 2229 Fragments arranged by size: 2281 2229 1545 860 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7012 PciI A'CATG_T Cuts at: 5911 5911 Size: 7012 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7012 PmeI GTTT'AAAC Cuts at: 4316 4316 Size: 7012 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5081 5113 6594 6626 5081 Size: 32 1481 32 5467 Fragments arranged by size: 5467 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7012 PsiI TTA'TAA Cuts at: 3954 4582 3954 Size: 628 6384 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2326 2358 2326 Size: 32 6980 PstI C_TGCA'G Cuts at: 830 4364 830 Size: 3534 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7012 SalI G'TCGA_C Cuts at: 1936 4354 1936 Size: 2418 4594 SapI GCTCTTCn'nnn_ Cuts at: 2269 2269 Size: 7012 SbfI CC_TGCA'GG Cuts at: 4364 4364 Size: 7012 ScaI AGT'ACT Cuts at: 5260 6972 5260 Size: 1712 5300 SfcI C'TryA_G Cuts at: 826 1045 2823 2894 4360 5495 6176 6367 826 Size: 219 1778 71 1466 1135 681 191 1471 Fragments arranged by size: 1778 1471 1466 1135 681 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7012 SmaI CCC'GGG Cuts at: 1872 4339 1872 Size: 2467 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2856 3666 4247 5116 6017 6279 Size: 197 926 913 810 581 869 901 262 Cuts at: 6279 820 Size: 1553 Fragments arranged by size: 1553 926 913 901 869 810 581 262 197 SnaBI TAC'GTA Cuts at: 493 4803 493 Size: 4310 2702 SpeI A'CTAG_T Cuts at: 152 2176 152 Size: 2024 4988 SphI G_CATG'C Cuts at: 1132 4370 1132 Size: 3238 3774 SspI AAT'ATT Cuts at: 5 52 3244 4936 5 Size: 47 3192 1692 2081 Fragments arranged by size: 3192 2081 1692 47 StuI AGG'CCT Cuts at: 1517 2672 1517 Size: 1155 5857 StyI C'CwwG_G Cuts at: 513 1065 3312 3640 4451 513 Size: 552 2247 328 811 3074 Fragments arranged by size: 3074 2247 811 552 328 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5039 5056 5209 5394 5839 325 Size: 1255 3459 17 153 185 445 1498 Fragments arranged by size: 3459 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3452 5258 6970 Size: 274 80 33 51 1443 1475 1806 1712 Cuts at: 6970 96 Size: 138 Fragments arranged by size: 1806 1712 1475 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2791 2823 6972 7004 2791 Size: 32 4149 32 2799 Fragments arranged by size: 4149 2799 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7012 XbaI T'CTAG_A Cuts at: 4348 4348 Size: 7012 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3779 3779 Size: 7012 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7012 XmaI C'CCGG_G Cuts at: 1870 4337 1870 Size: 2467 4545 XmnI GAAnn'nnTTC Cuts at: 2386 4221 5141 2386 Size: 1835 920 4257 Fragments arranged by size: 4257 1835 920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4820 276 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI