(Linear) (Six-base) MAPSORT of: KIEE2090.seq Check: 2205 from: 1 to: 2307 KIEE2090.orf With 182 enzymes: SgfI * July 3, 2012 17:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2238 2307 Size: 2238 69 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 167 593 1711 2000 2307 Size: 167 426 1118 289 307 Fragments arranged by size: 1118 426 307 289 167 AflII C'TTAA_G Cuts at: 0 2242 2307 Size: 2242 65 AflIII A'CryG_T Cuts at: 0 1181 2307 Size: 1181 1126 AgeI A'CCGG_T Cuts at: 0 1981 2307 Size: 1981 326 AleI CACnn'nnGTG Cuts at: 0 1566 2307 Size: 1566 741 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 464 2307 Size: 464 1843 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 209 241 1580 1612 2307 Size: 209 32 1339 32 695 Fragments arranged by size: 1339 695 209 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 517 2253 2307 Size: 517 1736 54 Fragments arranged by size: 1736 517 54 ApoI r'AATT_y Cuts at: 0 45 378 800 975 1146 1705 1757 1872 Size: 45 333 422 175 171 559 52 115 Cuts at: 1872 2124 2307 Size: 252 183 Fragments arranged by size: 559 422 333 252 183 175 171 115 52 45 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1154 1187 2307 Size: 1154 33 1120 Fragments arranged by size: 1154 1120 33 BanI G'GyrC_C Cuts at: 0 1630 2307 Size: 1630 677 BbsI GAAGACnn'nnnn_ Cuts at: 0 15 143 499 917 2244 2307 Size: 15 128 356 418 1327 63 Fragments arranged by size: 1327 418 356 128 63 15 BglI GCCn_nnn'nGGC Cuts at: 0 543 2307 Size: 543 1764 Bme1580I G_kGCm'C Cuts at: 0 1635 2307 Size: 1635 672 BmrI ACTGGGnnnn_n' Cuts at: 0 2182 2307 Size: 2182 125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 845 1151 1495 1799 2307 Size: 512 333 306 344 304 508 Fragments arranged by size: 512 508 344 333 306 304 Bpu10I CC'TnA_GC Cuts at: 0 1996 2307 Size: 1996 311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 836 1646 2307 Size: 836 810 661 Fragments arranged by size: 836 810 661 BsaI GGTCTCn'nnnn_ Cuts at: 0 1574 2307 Size: 1574 733 BsaWI w'CCGG_w Cuts at: 0 1981 2199 2307 Size: 1981 218 108 Fragments arranged by size: 1981 218 108 BseYI C'CCAG_C Cuts at: 0 1295 2307 Size: 1295 1012 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 2307 Size: 634 1673 BsmI GAATG_Cn' Cuts at: 0 846 1947 2307 Size: 846 1101 360 Fragments arranged by size: 1101 846 360 Bsp1286I G_dGCh'C Cuts at: 0 1635 2307 Size: 1635 672 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2238 2307 Size: 2238 69 BsrDI GCAATG_nn' Cuts at: 0 598 2307 Size: 598 1709 BsrFI r'CCGG_y Cuts at: 0 1981 2307 Size: 1981 326 BssSI C'ACGA_G Cuts at: 0 2043 2307 Size: 2043 264 BstXI CCAn_nnnn'nTGG Cuts at: 0 869 2307 Size: 869 1438 BstYI r'GATC_y Cuts at: 0 1595 2307 Size: 1595 712 Bsu36I CC'TnA_GG Cuts at: 0 651 2307 Size: 651 1656 BtgI C'CryG_G Cuts at: 0 1307 2307 Size: 1307 1000 ClaI AT'CG_AT Cuts at: 0 1019 2307 Size: 1019 1288 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 917 952 2307 Size: 917 35 1355 Fragments arranged by size: 1355 917 35 DraI TTT'AAA Cuts at: 0 953 1710 2307 Size: 953 757 597 Fragments arranged by size: 953 757 597 DrdI GACnn_nn'nnGTC Cuts at: 0 522 2307 Size: 522 1785 EaeI y'GGCC_r Cuts at: 0 1310 2307 Size: 1310 997 EarI CTCTTCn'nnn_ Cuts at: 0 264 1687 1921 2307 Size: 264 1423 234 386 Fragments arranged by size: 1423 386 264 234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 167 512 593 845 1151 1495 1711 1799 Size: 167 345 81 252 306 344 216 88 Cuts at: 1799 2000 2307 Size: 201 307 Fragments arranged by size: 345 344 307 306 252 216 201 167 88 81 EcoNI CCTnn'n_nnAGG Cuts at: 0 1729 2307 Size: 1729 578 EcoRI G'AATT_C Cuts at: 0 1146 1757 2124 2307 Size: 1146 611 367 183 Fragments arranged by size: 1146 611 367 183 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1277 2290 2307 Size: 1277 1013 17 Fragments arranged by size: 1277 1013 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1188 1220 1264 1296 1333 1365 1597 1629 Size: 1188 32 44 32 37 32 232 32 Cuts at: 1629 2307 Size: 678 Fragments arranged by size: 1188 678 232 44 37 32 32 32 32 MfeI C'AATT_G Cuts at: 0 280 829 2307 Size: 280 549 1478 Fragments arranged by size: 1478 549 280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1419 2307 Size: 1419 888 MscI TGG'CCA Cuts at: 0 1312 2307 Size: 1312 995 MslI CAynn'nnrTG Cuts at: 0 1566 2307 Size: 1566 741 NcoI C'CATG_G Cuts at: 0 1307 2307 Size: 1307 1000 NdeI CA'TA_TG Cuts at: 0 1373 2307 Size: 1373 934 NspI r_CATG'y Cuts at: 0 84 2307 Size: 84 2223 PsiI TTA'TAA Cuts at: 0 1949 2307 Size: 1949 358 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 321 353 2307 Size: 321 32 1954 Fragments arranged by size: 1954 321 32 SapI GCTCTTCn'nnn_ Cuts at: 0 264 2307 Size: 264 2043 SfcI C'TryA_G Cuts at: 0 818 889 2307 Size: 818 71 1418 Fragments arranged by size: 1418 818 71 SmlI C'TyrA_G Cuts at: 0 851 1661 2242 2307 Size: 851 810 581 65 Fragments arranged by size: 851 810 581 65 SpeI A'CTAG_T Cuts at: 0 171 2307 Size: 171 2136 SspI AAT'ATT Cuts at: 0 1239 2307 Size: 1239 1068 StuI AGG'CCT Cuts at: 0 667 2307 Size: 667 1640 StyI C'CwwG_G Cuts at: 0 1307 1635 2307 Size: 1307 328 672 Fragments arranged by size: 1307 672 328 TatI w'GTAC_w Cuts at: 0 1447 2307 Size: 1447 860 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 786 818 2307 Size: 786 32 1489 Fragments arranged by size: 1489 786 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1774 2307 Size: 1774 533 XmnI GAAnn'nnTTC Cuts at: 0 381 2216 2307 Size: 381 1835 91 Fragments arranged by size: 1835 381 91 Enzymes that do cut: AarI AcuI AflII AflIII AgeI AleI AlfI AloI AlwNI ApoI BaeI BanI BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI BseYI BsgI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI ClaI CspCI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI FalI Hin4I MfeI MmeI MscI MslI NcoI NdeI NspI PsiI PsrI SapI SfcI SmlI SpeI SspI StuI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AhdI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI