(Circular) (Six-base) MAPSORT of: pFN21AE3294.seq Check: 7239 from: 1 to: 7003 pFN21AE3294 7003 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2495 3058 2495 Size: 563 6440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3534 4140 4346 1937 Size: 1597 606 206 4594 Fragments arranged by size: 4594 1597 606 206 Acc65I G'GTAC_C Cuts at: 4324 4324 Size: 7003 AclI AA'CG_TT Cuts at: 1563 5130 5503 1563 Size: 3567 373 3063 Fragments arranged by size: 3567 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2200 2368 2509 2990 3206 3382 3608 3758 Size: 568 168 141 481 216 176 226 150 Cuts at: 3758 3838 5066 6450 6718 1632 Size: 80 1228 1384 268 1917 Fragments arranged by size: 1917 1384 1228 568 481 268 226 216 176 168 150 141 80 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6806 AflIII A'CryG_T Cuts at: 1203 3620 4293 5902 6879 1203 Size: 2417 673 1609 977 1327 Fragments arranged by size: 2417 1609 1327 977 673 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 5732 5732 Size: 7003 AleI CACnn'nnGTG Cuts at: 1166 2265 2396 1166 Size: 1099 131 5773 Fragments arranged by size: 5773 1099 131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3188 1184 Size: 2004 4999 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2834 2866 4047 4079 2834 Size: 32 1181 32 5758 Fragments arranged by size: 5758 1181 32 32 AlwNI CAG_nnn'CTG Cuts at: 2293 2317 2374 2497 2836 3073 3175 3763 4746 Size: 24 57 123 339 237 102 588 983 Cuts at: 4746 6318 2293 Size: 1572 2978 Fragments arranged by size: 2978 1572 983 588 339 237 123 102 57 24 ApaI G_GGCC'C Cuts at: 3184 4120 3184 Size: 936 6067 ApaLI G'TGCA_C Cuts at: 3503 5060 6216 3503 Size: 1557 1156 4290 Fragments arranged by size: 4290 1557 1156 ApoI r'AATT_y Cuts at: 1493 3271 3419 3743 4312 4538 4690 6799 1493 Size: 1778 148 324 569 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 569 324 226 152 148 AseI AT'TA_AT Cuts at: 160 1032 5557 160 Size: 872 4525 1606 Fragments arranged by size: 4525 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2766 2805 2990 3407 3941 4328 Size: 73 78 745 39 185 417 534 387 Cuts at: 4328 1870 Size: 4545 Fragments arranged by size: 4545 745 534 417 387 185 78 73 39 BamHI G'GATC_C Cuts at: 2256 4333 4700 2256 Size: 2077 367 4559 Fragments arranged by size: 4559 2077 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 4324 5779 618 Size: 325 217 627 285 2252 1455 1842 Fragments arranged by size: 2252 1842 1455 627 325 285 217 BanII G_rGCy'C Cuts at: 1391 2968 2997 3184 3447 3966 4120 4322 1391 Size: 1577 29 187 263 519 154 202 4072 Fragments arranged by size: 4072 1577 519 263 202 187 154 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3844 928 Size: 971 1945 4087 Fragments arranged by size: 4087 1945 971 BbvCI CC'TCA_GC Cuts at: 3815 3815 Size: 7003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5194 5228 1903 Size: 34 3257 34 3678 Fragments arranged by size: 3678 3257 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4895 6105 4895 Size: 1210 5793 BclI T'GATC_A Cuts at: 1548 4167 1548 Size: 2619 4384 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2415 3086 5614 Size: 107 122 71 631 605 743 671 2528 Cuts at: 5614 136 Size: 1525 Fragments arranged by size: 2528 1525 743 671 631 605 122 107 71 BglII A'GATC_T Cuts at: 2139 6998 2139 Size: 4859 2144 BlpI GC'TnA_GC Cuts at: 2816 3452 4420 2816 Size: 636 968 5399 Fragments arranged by size: 5399 968 636 Bme1580I G_kGCm'C Cuts at: 1790 3184 3507 3655 4120 5064 6220 1790 Size: 1394 323 148 465 944 1156 2573 Fragments arranged by size: 2573 1394 1156 944 465 323 148 BmgBI CAC'GTC Cuts at: 1318 4296 1318 Size: 2978 4025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2653 3832 4114 Size: 416 53 83 399 24 1236 1179 282 Cuts at: 4114 4734 5692 442 Size: 620 958 1753 Fragments arranged by size: 1753 1236 1179 958 620 416 399 282 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2792 3133 3301 2792 Size: 341 168 6494 Fragments arranged by size: 6494 341 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2764 3667 5663 1239 Size: 1076 449 903 1996 2579 Fragments arranged by size: 2579 1996 1076 903 449 Bpu10I CC'TnA_GC Cuts at: 3815 3815 Size: 7003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2425 3191 5128 5993 6291 2425 Size: 766 1937 865 298 3137 Fragments arranged by size: 3137 1937 865 766 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2093 2763 4223 5666 882 Size: 617 594 670 1460 1443 2219 Fragments arranged by size: 2219 1460 1443 670 617 594 BsaAI yAC'GTr Cuts at: 493 1204 4794 493 Size: 711 3590 2702 Fragments arranged by size: 3590 2702 711 BsaBI GATnn'nnATC Cuts at: 6997 6997 Size: 7003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3912 3954 4810 Size: 53 83 186 936 476 1903 42 856 Cuts at: 4810 5192 5827 275 Size: 382 635 1451 Fragments arranged by size: 1903 1451 936 856 635 476 382 186 83 53 42 BsaWI w'CCGG_w Cuts at: 2116 5436 5834 6108 6255 6666 2116 Size: 3320 398 274 147 411 2453 Fragments arranged by size: 3320 2453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2762 2792 4047 4077 1178 Size: 30 165 30 1359 30 1255 30 4104 Fragments arranged by size: 4104 1359 1255 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2392 2453 2456 3425 3989 4295 1186 Size: 1206 61 3 969 564 306 3894 Fragments arranged by size: 3894 1206 969 564 306 61 3 BseYI C'CCAG_C Cuts at: 2132 2836 3966 4011 6206 6702 2132 Size: 704 1130 45 2195 496 2433 Fragments arranged by size: 2433 2195 1130 704 496 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 2723 2753 2849 3386 1163 Size: 573 564 423 30 96 537 4780 Fragments arranged by size: 4780 573 564 537 423 96 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2594 4218 4453 5214 6242 1540 Size: 275 186 593 1624 235 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 593 275 235 186 BsiHKAI G_wGCw'C Cuts at: 3102 3447 3507 4263 5064 5149 6220 3102 Size: 345 60 756 801 85 1071 3885 Fragments arranged by size: 3885 1071 801 756 345 85 60 BsmI GAATG_Cn' Cuts at: 2628 3082 4514 4607 2628 Size: 454 1432 93 5024 Fragments arranged by size: 5024 1432 454 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2968 2997 3102 3184 3447 3507 3655 Size: 399 1178 29 105 82 263 60 148 Cuts at: 3655 3966 4120 4263 4322 5064 5149 6220 1391 Size: 311 154 143 59 742 85 1071 2174 Fragments arranged by size: 2174 1178 1071 742 399 311 263 154 148 143 105 85 82 60 59 29 BspEI T'CCGG_A Cuts at: 2116 6666 2116 Size: 4550 2453 BspHI T'CATG_A Cuts at: 4892 6622 4892 Size: 1730 5273 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2495 3058 3304 4358 844 Size: 1250 401 563 246 1054 3489 Fragments arranged by size: 3489 1250 1054 563 401 246 BsrBI CCG'CTC Cuts at: 1394 1823 3254 3285 4890 1394 Size: 429 1431 31 1605 3507 Fragments arranged by size: 3507 1605 1431 429 31 BsrDI GCAATG_nn' Cuts at: 65 1248 3520 5498 5672 65 Size: 1183 2272 1978 174 1396 Fragments arranged by size: 2272 1978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 3102 3309 5647 5834 1700 Size: 110 338 954 207 2338 187 2869 Fragments arranged by size: 2869 2338 954 338 207 187 110 BsrGI T'GTAC_A Cuts at: 96 2420 96 Size: 2324 4679 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7003 BssSI C'ACGA_G Cuts at: 2204 4130 5063 6075 2204 Size: 1926 933 1012 3132 Fragments arranged by size: 3132 1926 1012 933 BstAPI GCAn_nnn'nTGC Cuts at: 794 2746 3763 794 Size: 1952 1017 4034 Fragments arranged by size: 4034 1952 1017 BstBI TT'CG_AA Cuts at: 5807 5807 Size: 7003 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7003 BstXI CCAn_nnnn'nTGG Cuts at: 4240 4240 Size: 7003 BstYI r'GATC_y Cuts at: 1972 2139 2256 4333 4700 5085 5102 6554 6640 Size: 167 117 2077 367 385 17 1452 86 Cuts at: 6640 6652 6998 1972 Size: 12 346 1977 Fragments arranged by size: 2077 1977 1452 385 367 346 167 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3555 513 Size: 552 939 1551 3961 Fragments arranged by size: 3961 1551 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3130 487 Size: 683 56 1904 4360 Fragments arranged by size: 4360 1904 683 56 BtsI GCAGTG_nn' Cuts at: 4261 4519 5313 5333 4261 Size: 258 794 20 5931 Fragments arranged by size: 5931 794 258 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3028 3063 529 Size: 35 2464 35 4469 Fragments arranged by size: 4469 2464 35 35 DraI TTT'AAA Cuts at: 4276 4307 4276 Size: 31 6972 DraIII CAC_nnn'GTG Cuts at: 2661 2661 Size: 7003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2153 6010 809 Size: 507 837 3857 1802 Fragments arranged by size: 3857 1802 837 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2591 2866 4450 Size: 54 1425 50 275 18 761 275 1584 Cuts at: 4450 5339 6968 8 Size: 889 1629 43 Fragments arranged by size: 1629 1584 1425 889 761 275 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2591 4450 1537 Size: 275 779 1859 4090 Fragments arranged by size: 4090 1859 779 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2417 2901 2966 3057 3263 3728 4933 1353 Size: 1064 484 65 91 206 465 1205 3423 Fragments arranged by size: 3423 1205 1064 484 465 206 91 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2344 4055 5590 5964 6110 387 Size: 1064 138 755 1711 1535 374 146 1280 Fragments arranged by size: 1711 1535 1280 1064 755 374 146 138 EcoICRI GAG'CTC Cuts at: 3445 3445 Size: 7003 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2315 2368 2509 2764 2990 3206 Size: 393 568 115 53 141 255 226 216 Cuts at: 3206 3382 3608 3667 3758 3838 5066 5663 6450 Size: 176 226 59 91 80 1228 597 787 Cuts at: 6450 6718 1239 Size: 268 1524 Fragments arranged by size: 1524 1228 787 597 568 393 268 255 226 226 216 176 141 115 91 80 59 53 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2279 4076 2267 Size: 12 1797 5194 Fragments arranged by size: 5194 1797 12 EcoO109I rG'GnC_Cy Cuts at: 2272 2290 2314 2569 3181 4117 2272 Size: 18 24 255 612 936 5158 Fragments arranged by size: 5158 936 612 255 24 18 EcoRI G'AATT_C Cuts at: 3419 3743 4312 4690 3419 Size: 324 569 378 5732 Fragments arranged by size: 5732 569 378 324 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3586 3586 Size: 7003 FspI TGC'GCA Cuts at: 2060 5509 2060 Size: 3449 3554 HaeII r_GCGC'y Cuts at: 1678 3252 6150 1678 Size: 1574 2898 2531 Fragments arranged by size: 2898 2531 1574 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2336 2368 2762 2794 4007 Size: 32 135 32 764 32 394 32 1213 Cuts at: 4007 4039 5644 5676 5718 5750 1373 Size: 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 1213 764 394 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 3439 4347 4593 669 Size: 988 281 541 960 908 246 3079 Fragments arranged by size: 3079 988 960 908 541 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7003 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KpnI G_GTAC'C Cuts at: 4328 4328 Size: 7003 MfeI C'AATT_G Cuts at: 4602 4602 Size: 7003 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2733 3846 6117 6301 1305 Size: 732 696 1113 2271 184 2007 Fragments arranged by size: 2271 2007 1113 732 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2868 10 Size: 54 1425 343 1036 4145 Fragments arranged by size: 4145 1425 1036 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2265 2396 3219 3236 4961 5320 5479 Size: 648 1099 131 823 17 1725 359 159 Cuts at: 5479 518 Size: 2042 Fragments arranged by size: 2042 1725 1099 823 648 359 159 131 17 MspA1I CmG'CkG Cuts at: 1606 2737 2980 3172 3370 3493 4419 5096 6244 Size: 1131 243 192 198 123 926 677 1148 Cuts at: 6244 6489 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1131 926 677 245 243 198 192 123 NaeI GCC'GGC Cuts at: 3311 3311 Size: 7003 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5512 Fragments arranged by size: 5512 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7003 NgoMIV G'CCGG_C Cuts at: 3309 3309 Size: 7003 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7003 NotI GC'GGCC_GC Cuts at: 2591 4450 2591 Size: 1859 5144 NspI r_CATG'y Cuts at: 1132 1229 3624 3994 4361 5906 1132 Size: 97 2395 370 367 1545 2229 Fragments arranged by size: 2395 2229 1545 370 367 97 PasI CC'CwG_GG Cuts at: 1348 2077 2450 4078 1348 Size: 729 373 1628 4273 Fragments arranged by size: 4273 1628 729 373 PciI A'CATG_T Cuts at: 3620 5902 3620 Size: 2282 4721 PflMI CCAn_nnn'nTGG Cuts at: 1108 2293 2395 2497 3634 1108 Size: 1185 102 102 1137 4477 Fragments arranged by size: 4477 1185 1137 102 102 PfoI T'CCnGG_A Cuts at: 3644 3726 3644 Size: 82 6921 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4047 4079 5072 5104 6585 6617 4047 Size: 32 993 32 1481 32 4433 Fragments arranged by size: 4433 1481 993 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2272 2290 2314 2569 2272 Size: 18 24 255 6706 Fragments arranged by size: 6706 255 24 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7003 PsiI TTA'TAA Cuts at: 4573 4573 Size: 7003 PspOMI G'GGCC_C Cuts at: 3180 4116 3180 Size: 936 6067 PstI C_TGCA'G Cuts at: 830 2370 2891 2985 4268 4355 830 Size: 1540 521 94 1283 87 3478 Fragments arranged by size: 3478 1540 1283 521 94 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 PvuII CAG'CTG Cuts at: 2737 3172 2737 Size: 435 6568 SacI G_AGCT'C Cuts at: 3447 3447 Size: 7003 SalI G'TCGA_C Cuts at: 1936 4345 1936 Size: 2409 4594 SanDI GG'GwC_CC Cuts at: 2569 2569 Size: 7003 SapI GCTCTTCn'nnn_ Cuts at: 3057 3728 3057 Size: 671 6332 SbfI CC_TGCA'GG Cuts at: 4355 4355 Size: 7003 ScaI AGT'ACT Cuts at: 4154 5251 6963 4154 Size: 1097 1712 4194 Fragments arranged by size: 4194 1712 1097 SexAI A'CCwGG_T Cuts at: 2274 2316 3627 2274 Size: 42 1311 5650 Fragments arranged by size: 5650 1311 42 SfcI C'TryA_G Cuts at: 826 1045 2366 2887 2981 4264 4351 5486 6167 Size: 219 1321 521 94 1283 87 1135 681 Cuts at: 6167 6358 826 Size: 191 1471 Fragments arranged by size: 1471 1321 1283 1135 681 521 219 191 94 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7003 SmaI CCC'GGG Cuts at: 1872 4330 1872 Size: 2458 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2404 3206 5107 6008 6270 820 Size: 197 926 461 802 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 802 461 262 197 SnaBI TAC'GTA Cuts at: 493 4794 493 Size: 4301 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7003 SphI G_CATG'C Cuts at: 1132 4361 1132 Size: 3229 3774 SspI AAT'ATT Cuts at: 5 52 4927 5 Size: 47 4875 2081 Fragments arranged by size: 4875 2081 47 StuI AGG'CCT Cuts at: 1517 3089 3820 3947 1517 Size: 1572 731 127 4573 Fragments arranged by size: 4573 1572 731 127 StyI C'CwwG_G Cuts at: 513 1065 2004 2613 4233 4442 513 Size: 552 939 609 1620 209 3074 Fragments arranged by size: 3074 1620 939 609 552 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2672 5030 5047 5200 5385 5830 325 Size: 1255 1092 2358 17 153 185 445 1498 Fragments arranged by size: 2358 1498 1255 1092 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2042 2420 4152 Size: 274 80 33 51 1443 65 378 1732 Cuts at: 4152 5249 6961 96 Size: 1097 1712 138 Fragments arranged by size: 1732 1712 1443 1097 378 274 138 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3013 3045 3977 4009 6963 6995 3013 Size: 32 932 32 2954 32 3021 Fragments arranged by size: 3021 2954 932 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3629 1316 Size: 2313 4690 XbaI T'CTAG_A Cuts at: 4298 4339 4298 Size: 41 6962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7003 XmaI C'CCGG_G Cuts at: 1870 4328 1870 Size: 2458 4545 XmnI GAAnn'nnTTC Cuts at: 3722 3743 4059 5132 3722 Size: 21 316 1073 5593 Fragments arranged by size: 5593 1073 316 21 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI