(Linear) (Six-base) MAPSORT of: KIEE3294.seq Check: 2266 from: 1 to: 2298 KIEE3294.orf With 182 enzymes: SgfI * July 3, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 490 1053 2298 Size: 490 563 1245 Fragments arranged by size: 1245 563 490 AccI GT'mk_AC Cuts at: 0 1529 2135 2298 Size: 1529 606 163 Fragments arranged by size: 1529 606 163 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 363 504 985 1201 1377 1603 1753 Size: 195 168 141 481 216 176 226 150 Cuts at: 1753 1833 2298 Size: 80 465 Fragments arranged by size: 481 465 226 216 195 176 168 150 141 80 AflIII A'CryG_T Cuts at: 0 1615 2288 2298 Size: 1615 673 10 Fragments arranged by size: 1615 673 10 AleI CACnn'nnGTG Cuts at: 0 260 391 2298 Size: 260 131 1907 Fragments arranged by size: 1907 260 131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1183 2298 Size: 1183 1115 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 829 861 2042 2074 2298 Size: 829 32 1181 32 224 Fragments arranged by size: 1181 829 224 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 288 312 369 492 831 1068 1170 1758 Size: 288 24 57 123 339 237 102 588 Cuts at: 1758 2298 Size: 540 Fragments arranged by size: 588 540 339 288 237 123 102 57 24 ApaI G_GGCC'C Cuts at: 0 1179 2115 2298 Size: 1179 936 183 Fragments arranged by size: 1179 936 183 ApaLI G'TGCA_C Cuts at: 0 1498 2298 Size: 1498 800 ApoI r'AATT_y Cuts at: 0 1266 1414 1738 2298 Size: 1266 148 324 560 Fragments arranged by size: 1266 560 324 148 AvaI C'yCGr_G Cuts at: 0 16 761 800 985 1402 1936 2298 Size: 16 745 39 185 417 534 362 Fragments arranged by size: 745 534 417 362 185 39 16 BamHI G'GATC_C Cuts at: 0 251 2298 Size: 251 2047 BanI G'GyrC_C Cuts at: 0 67 2298 Size: 67 2231 BanII G_rGCy'C Cuts at: 0 963 992 1179 1442 1961 2115 2298 Size: 963 29 187 263 519 154 183 Fragments arranged by size: 963 519 263 187 183 154 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 1839 2298 Size: 1839 459 BbvCI CC'TCA_GC Cuts at: 0 1810 2298 Size: 1810 488 BclI T'GATC_A Cuts at: 0 2162 2298 Size: 2162 136 BglI GCCn_nnn'nGGC Cuts at: 0 410 1081 2298 Size: 410 671 1217 Fragments arranged by size: 1217 671 410 BglII A'GATC_T Cuts at: 0 134 2298 Size: 134 2164 BlpI GC'TnA_GC Cuts at: 0 811 1447 2298 Size: 811 636 851 Fragments arranged by size: 851 811 636 Bme1580I G_kGCm'C Cuts at: 0 1179 1502 1650 2115 2298 Size: 1179 323 148 465 183 Fragments arranged by size: 1179 465 323 183 148 BmgBI CAC'GTC Cuts at: 0 2291 2298 Size: 2291 7 BmrI ACTGGGnnnn_n' Cuts at: 0 648 1827 2109 2298 Size: 648 1179 282 189 Fragments arranged by size: 1179 648 282 189 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 787 1128 1296 2298 Size: 787 341 168 1002 Fragments arranged by size: 1002 787 341 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 759 1662 2298 Size: 310 449 903 636 Fragments arranged by size: 903 636 449 310 Bpu10I CC'TnA_GC Cuts at: 0 1810 2298 Size: 1810 488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 1186 2298 Size: 420 766 1112 Fragments arranged by size: 1112 766 420 BsaI GGTCTCn'nnnn_ Cuts at: 0 88 758 2218 2298 Size: 88 670 1460 80 Fragments arranged by size: 1460 670 88 80 BsaHI Gr'CG_yC Cuts at: 0 4 1907 1949 2298 Size: 4 1903 42 349 Fragments arranged by size: 1903 349 42 4 BsaWI w'CCGG_w Cuts at: 0 111 2298 Size: 111 2187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 757 787 2042 2072 2298 Size: 757 30 1255 30 226 Fragments arranged by size: 1255 757 226 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 387 448 451 1420 1984 2290 2298 Size: 387 61 3 969 564 306 8 Fragments arranged by size: 969 564 387 306 61 8 3 BseYI C'CCAG_C Cuts at: 0 127 831 1961 2006 2298 Size: 127 704 1130 45 292 Fragments arranged by size: 1130 704 292 127 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 718 748 844 1381 2298 Size: 295 423 30 96 537 917 Fragments arranged by size: 917 537 423 295 96 30 BsiEI CG_ry'CG Cuts at: 0 589 2213 2298 Size: 589 1624 85 Fragments arranged by size: 1624 589 85 BsiHKAI G_wGCw'C Cuts at: 0 1097 1442 1502 2258 2298 Size: 1097 345 60 756 40 Fragments arranged by size: 1097 756 345 60 40 BsmI GAATG_Cn' Cuts at: 0 623 1077 2298 Size: 623 454 1221 Fragments arranged by size: 1221 623 454 Bsp1286I G_dGCh'C Cuts at: 0 963 992 1097 1179 1442 1502 1650 1961 Size: 963 29 105 82 263 60 148 311 Cuts at: 1961 2115 2258 2298 Size: 154 143 40 Fragments arranged by size: 963 311 263 154 148 143 105 82 60 40 29 BspEI T'CCGG_A Cuts at: 0 111 2298 Size: 111 2187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 89 490 1053 1299 2298 Size: 89 401 563 246 999 Fragments arranged by size: 999 563 401 246 89 BsrBI CCG'CTC Cuts at: 0 1249 1280 2298 Size: 1249 31 1018 Fragments arranged by size: 1249 1018 31 BsrDI GCAATG_nn' Cuts at: 0 1515 2298 Size: 1515 783 BsrFI r'CCGG_y Cuts at: 0 143 1097 1304 2298 Size: 143 954 207 994 Fragments arranged by size: 994 954 207 143 BsrGI T'GTAC_A Cuts at: 0 415 2298 Size: 415 1883 BssSI C'ACGA_G Cuts at: 0 199 2125 2298 Size: 199 1926 173 Fragments arranged by size: 1926 199 173 BstAPI GCAn_nnn'nTGC Cuts at: 0 741 1758 2298 Size: 741 1017 540 Fragments arranged by size: 1017 741 540 BstXI CCAn_nnnn'nTGG Cuts at: 0 2235 2298 Size: 2235 63 BstYI r'GATC_y Cuts at: 0 134 251 2298 Size: 134 117 2047 Fragments arranged by size: 2047 134 117 BtgI C'CryG_G Cuts at: 0 1550 2298 Size: 1550 748 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1125 2298 Size: 1125 1173 BtsI GCAGTG_nn' Cuts at: 0 2256 2298 Size: 2256 42 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1023 1058 2298 Size: 1023 35 1240 Fragments arranged by size: 1240 1023 35 DraI TTT'AAA Cuts at: 0 2271 2298 Size: 2271 27 DraIII CAC_nnn'GTG Cuts at: 0 656 2298 Size: 656 1642 DrdI GACnn_nn'nnGTC Cuts at: 0 148 2298 Size: 148 2150 EaeI y'GGCC_r Cuts at: 0 586 861 2298 Size: 586 275 1437 Fragments arranged by size: 1437 586 275 EagI C'GGCC_G Cuts at: 0 586 2298 Size: 586 1712 EarI CTCTTCn'nnn_ Cuts at: 0 412 896 961 1052 1258 1723 2298 Size: 412 484 65 91 206 465 575 Fragments arranged by size: 575 484 465 412 206 91 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 339 2050 2298 Size: 339 1711 248 Fragments arranged by size: 1711 339 248 EcoICRI GAG'CTC Cuts at: 0 1440 2298 Size: 1440 858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 310 363 504 759 985 1201 1377 Size: 195 115 53 141 255 226 216 176 Cuts at: 1377 1603 1662 1753 1833 2298 Size: 226 59 91 80 465 Fragments arranged by size: 465 255 226 226 216 195 176 141 115 91 80 59 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 262 274 2071 2298 Size: 262 12 1797 227 Fragments arranged by size: 1797 262 227 12 EcoO109I rG'GnC_Cy Cuts at: 0 267 285 309 564 1176 2112 2298 Size: 267 18 24 255 612 936 186 Fragments arranged by size: 936 612 267 255 186 24 18 EcoRI G'AATT_C Cuts at: 0 1414 1738 2298 Size: 1414 324 560 Fragments arranged by size: 1414 560 324 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1581 2298 Size: 1581 717 FspI TGC'GCA Cuts at: 0 55 2298 Size: 55 2243 HaeII r_GCGC'y Cuts at: 0 1247 2298 Size: 1247 1051 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 331 363 757 789 2002 2034 2298 Size: 331 32 394 32 1213 32 264 Fragments arranged by size: 1213 394 331 264 32 32 32 HincII GTy'rAC Cuts at: 0 474 1434 2298 Size: 474 960 864 Fragments arranged by size: 960 864 474 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 32 728 1841 2298 Size: 32 696 1113 457 Fragments arranged by size: 1113 696 457 32 MscI TGG'CCA Cuts at: 0 863 2298 Size: 863 1435 MslI CAynn'nnrTG Cuts at: 0 260 391 1214 1231 2298 Size: 260 131 823 17 1067 Fragments arranged by size: 1067 823 260 131 17 MspA1I CmG'CkG Cuts at: 0 732 975 1167 1365 1488 2298 Size: 732 243 192 198 123 810 Fragments arranged by size: 810 732 243 198 192 123 NaeI GCC'GGC Cuts at: 0 1306 2298 Size: 1306 992 NgoMIV G'CCGG_C Cuts at: 0 1304 2298 Size: 1304 994 NotI GC'GGCC_GC Cuts at: 0 586 2298 Size: 586 1712 NspI r_CATG'y Cuts at: 0 1619 1989 2298 Size: 1619 370 309 Fragments arranged by size: 1619 370 309 PasI CC'CwG_GG Cuts at: 0 72 445 2073 2298 Size: 72 373 1628 225 Fragments arranged by size: 1628 373 225 72 PciI A'CATG_T Cuts at: 0 1615 2298 Size: 1615 683 PflMI CCAn_nnn'nTGG Cuts at: 0 288 390 492 1629 2298 Size: 288 102 102 1137 669 Fragments arranged by size: 1137 669 288 102 102 PfoI T'CCnGG_A Cuts at: 0 1639 1721 2298 Size: 1639 82 577 Fragments arranged by size: 1639 577 82 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2042 2074 2298 Size: 2042 32 224 Fragments arranged by size: 2042 224 32 PpuMI rG'GwC_Cy Cuts at: 0 267 285 309 564 2298 Size: 267 18 24 255 1734 Fragments arranged by size: 1734 267 255 24 18 PspOMI G'GGCC_C Cuts at: 0 1175 2111 2298 Size: 1175 936 187 Fragments arranged by size: 1175 936 187 PstI C_TGCA'G Cuts at: 0 365 886 980 2263 2298 Size: 365 521 94 1283 35 Fragments arranged by size: 1283 521 365 94 35 PvuII CAG'CTG Cuts at: 0 732 1167 2298 Size: 732 435 1131 Fragments arranged by size: 1131 732 435 SacI G_AGCT'C Cuts at: 0 1442 2298 Size: 1442 856 SanDI GG'GwC_CC Cuts at: 0 564 2298 Size: 564 1734 SapI GCTCTTCn'nnn_ Cuts at: 0 1052 1723 2298 Size: 1052 671 575 Fragments arranged by size: 1052 671 575 ScaI AGT'ACT Cuts at: 0 2149 2298 Size: 2149 149 SexAI A'CCwGG_T Cuts at: 0 269 311 1622 2298 Size: 269 42 1311 676 Fragments arranged by size: 1311 676 269 42 SfcI C'TryA_G Cuts at: 0 361 882 976 2259 2298 Size: 361 521 94 1283 39 Fragments arranged by size: 1283 521 361 94 39 SmlI C'TyrA_G Cuts at: 0 399 1201 2298 Size: 399 802 1097 Fragments arranged by size: 1097 802 399 StuI AGG'CCT Cuts at: 0 1084 1815 1942 2298 Size: 1084 731 127 356 Fragments arranged by size: 1084 731 356 127 StyI C'CwwG_G Cuts at: 0 608 2228 2298 Size: 608 1620 70 Fragments arranged by size: 1620 608 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 667 2298 Size: 667 1631 TatI w'GTAC_w Cuts at: 0 37 415 2147 2298 Size: 37 378 1732 151 Fragments arranged by size: 1732 378 151 37 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1008 1040 1972 2004 2298 Size: 1008 32 932 32 294 Fragments arranged by size: 1008 932 294 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1624 2298 Size: 1624 674 XbaI T'CTAG_A Cuts at: 0 2293 2298 Size: 2293 5 XmnI GAAnn'nnTTC Cuts at: 0 1717 1738 2054 2298 Size: 1717 21 316 244 Fragments arranged by size: 1717 316 244 21 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV NotI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BmtI BsaAI BsaBI BsiWI BsmBI BspHI BssHII BstBI BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XcmI XhoI XmaI ZraI