################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccI AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MaeII MamI MluI MluNI MscI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 940 GGTACC G^GTACC AccII 523 CGCG CG^CG AccIII 713 TCCGGA T^CCGGA AcyI 1813 GACGCC G[GA]^CG[CT]C AflII 514 CTTAAG C^TTAAG AhdI 1575 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1044 CCTCAGG CC^T[ACGT]AGG Asp700I 856 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 940 GGTACC G^GTACC AspEI 1575 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 892 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 709 GAGCTC G[GA]GC[CT]^C BbvCI 1102 GCTGAGG CCTCAGC(-5/-2) BfrI 514 CTTAAG C^TTAAG BsaHI 1813 GACGCC G[GA]^CG[CT]C BsaWI 713 TCCGGA [AT]^CCGG[AT] BseAI 713 TCCGGA T^CCGGA BsiYI 1157 CCATCAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1157 CCATCAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspEI 713 TCCGGA T^CCGGA BspHI 864 TCATGA T^CATGA BsrBI 945 CCGCTC CCGCTC(-3/-3) BsrFI 611 ACCGGC [GA]^CCGG[CT] BstUI 523 CGCG CG^CG Bsu36I 1044 CCTCAGG CC^T[ACGT]AGG CfoI 754 GCGC GCG^C Cfr10I 611 ACCGGC [GA]^CCGG[CT] DraIII 902 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1370 CCATGG C^C[GA][CT]GG Eam1105I 1575 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1044 CCTCAGG CC^T[ACGT]AGG HaeII 753 GGCGCT [GA]GCGC^[CT] HgaI 1813 GACGC GACGC(5/10) HhaI 754 GCGC GCG^C Hin1I 1813 GACGCC G[GA]^CG[CT]C HinP1I 754 GCGC G^CGC KpnI 940 GGTACC GGTAC^C MfeI 2069 CAATTG C^AATTG MroI 713 TCCGGA T^CCGGA MunI 2069 CAATTG C^AATTG MvnI 523 CGCG CG^CG NcoI 1370 CCATGG C^CATGG PflFI 892 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 864 TCATGA T^CATGA SacI 709 GAGCTC GAGCT^C SspI 499 AATATT AAT^ATT StuI 1557 AGGCCT AGG^CCT Tth111I 892 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 856 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 1844 AAATTC [GA]^AATT[CT] 1900 AAATTC AfaI 941 GTAC GT^AC 1007 GTAC AlwNI 1271 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 1601 CAGCTGCTG ApoI 1844 AAATTC [GA]^AATT[CT] 1900 AAATTC AspHI 3 GAGCAC G[AT]GC[AT]^C 709 GAGCTC BciVI 804 GGATAC GTATCC(6/5) 1894 GTATCC BmyI 3 GAGCAC G[AGT]GC[ACT]^C 709 GAGCTC BsgI 1309 GTGCAG GTGCAG(16/14) 1687 GTGCAG BsiHKAI 3 GAGCAC G[AT]GC[AT]^C 709 GAGCTC BsmAI 846 GAGAC GTCTC(1/5) 2037 GTCTC Bsp1286I 3 GAGCAC G[AGT]GC[ACT]^C 709 GAGCTC Cac8I 520 GCACGC GC[ACGT]^[ACGT]GC 1333 GCCCGC FauI 944 CCCGC CCCGC(4/6) 1334 CCCGC HaeIII 614 GGCC GG^CC 1558 GGCC HapII 612 CCGG C^CGG 714 CCGG HincII 870 GTTGAC GT[CT]^[GA]AC 1435 GTTGAC HindII 870 GTTGAC GT[CT]^[GA]AC 1435 GTTGAC HpaII 612 CCGG C^CGG 714 CCGG MspA1I 920 CAGCTG C[AC]G^C[GT]G 1601 CAGCTG MspI 612 CCGG C^CGG 714 CCGG NspI 647 GCATGT [GA]CATG^[CT] 1314 GCATGT PvuII 920 CAGCTG CAG^CTG 1601 CAGCTG RsaI 941 GTAC GT^AC 1007 GTAC TaqI 1772 TCGA T^CGA 2258 TCGA TthHB8I 1772 TCGA T^CGA 2258 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI AvaII BanI BbvI BclI BfaI BpmI BsaJI BsmFI BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII EcoT14I FbaI Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII