(Circular) (Six-base) MAPSORT of: pFN21AB6251.seq Check: 2258 from: 1 to: 6985 pFN21AB6251 6985 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4795 278 Size: 53 83 186 936 3259 2468 Fragments arranged by size: 3259 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4328 1937 Size: 2391 4594 Acc65I G'GTAC_C Cuts at: 2945 4306 2945 Size: 1361 5624 AclI AA'CG_TT Cuts at: 1563 5112 5485 1563 Size: 3549 373 3063 Fragments arranged by size: 3549 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2712 3402 5048 6432 6700 1632 Size: 1080 690 1646 1384 268 1917 Fragments arranged by size: 1917 1646 1384 1080 690 268 AflII C'TTAA_G Cuts at: 820 1017 2519 820 Size: 197 1502 5286 Fragments arranged by size: 5286 1502 197 AflIII A'CryG_T Cuts at: 1203 5884 6861 1203 Size: 4681 977 1327 Fragments arranged by size: 4681 1327 977 AgeI A'CCGG_T Cuts at: 5816 5816 Size: 6985 AhdI GACnn_n'nnGTC Cuts at: 3585 5714 3585 Size: 2129 4856 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6985 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3182 3214 3270 3302 3182 Size: 32 56 32 6865 Fragments arranged by size: 6865 56 32 32 AlwNI CAG_nnn'CTG Cuts at: 3281 3611 4728 6300 3281 Size: 330 1117 1572 3966 Fragments arranged by size: 3966 1572 1117 330 ApaLI G'TGCA_C Cuts at: 5042 6198 5042 Size: 1156 5829 ApoI r'AATT_y Cuts at: 1493 3849 3905 4294 4520 4672 6781 1493 Size: 2356 56 389 226 152 2109 1697 Fragments arranged by size: 2356 2109 1697 389 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 5539 160 Size: 872 4507 1606 Fragments arranged by size: 4507 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 AvaI C'yCGr_G Cuts at: 1870 1943 4310 1870 Size: 73 2367 4545 Fragments arranged by size: 4545 2367 73 BamHI G'GATC_C Cuts at: 4315 4682 4315 Size: 367 6618 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2793 2945 3530 4306 5761 Size: 325 217 627 1006 152 585 776 1455 Cuts at: 5761 618 Size: 1842 Fragments arranged by size: 1842 1455 1006 776 627 585 325 217 152 BanII G_rGCy'C Cuts at: 1391 2718 4304 1391 Size: 1327 1586 4072 Fragments arranged by size: 4072 1586 1327 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6014 BbvCI CC'TCA_GC Cuts at: 3108 3108 Size: 6985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5176 5210 1903 Size: 34 3239 34 3678 Fragments arranged by size: 3678 3239 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2803 3910 4877 6087 2803 Size: 1107 967 1210 3701 Fragments arranged by size: 3701 1210 1107 967 BclI T'GATC_A Cuts at: 1548 2185 2248 4066 1548 Size: 637 63 1818 4467 Fragments arranged by size: 4467 1818 637 63 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5596 136 Size: 107 122 71 631 605 3924 1525 Fragments arranged by size: 3924 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6980 6980 Size: 6985 BlpI GC'TnA_GC Cuts at: 4402 4402 Size: 6985 Bme1580I G_kGCm'C Cuts at: 1790 5046 6202 1790 Size: 3256 1156 2573 Fragments arranged by size: 3256 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6985 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2749 3103 4716 Size: 416 53 83 399 24 1332 354 1613 Cuts at: 4716 5674 442 Size: 958 1753 Fragments arranged by size: 1753 1613 1332 958 416 399 354 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3303 3510 3798 5645 1239 Size: 2064 207 288 1847 2579 Fragments arranged by size: 2579 2064 1847 288 207 Bpu10I CC'TnA_GC Cuts at: 3108 3807 3108 Size: 699 6286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2693 3538 5110 5975 6273 2693 Size: 845 1572 865 298 3405 Fragments arranged by size: 3405 1572 865 845 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5648 882 Size: 617 4149 2219 Fragments arranged by size: 4149 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4776 493 Size: 711 3572 2702 Fragments arranged by size: 3572 2702 711 BsaBI GATnn'nnATC Cuts at: 6979 6979 Size: 6985 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3819 4792 5174 5809 Size: 53 83 186 936 2286 973 382 635 Cuts at: 5809 275 Size: 1451 Fragments arranged by size: 2286 1451 973 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2718 5418 5816 6090 6237 6648 2718 Size: 2700 398 274 147 411 3055 Fragments arranged by size: 3055 2700 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3508 3538 1178 Size: 30 165 30 2105 30 4625 Fragments arranged by size: 4625 2105 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6985 BseYI C'CCAG_C Cuts at: 6188 6684 6188 Size: 496 6489 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3335 3713 1163 Size: 573 1599 378 4435 Fragments arranged by size: 4435 1599 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4435 5196 6224 1540 Size: 275 186 2434 761 1028 2301 Fragments arranged by size: 2434 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2718 5046 5131 6202 2012 Size: 706 2328 85 1071 2795 Fragments arranged by size: 2795 2328 1071 706 85 BsmI GAATG_Cn' Cuts at: 4496 4589 4496 Size: 93 6892 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2718 4304 5046 5131 6202 1391 Size: 399 222 706 1586 742 85 1071 2174 Fragments arranged by size: 2174 1586 1071 742 706 399 222 85 BspEI T'CCGG_A Cuts at: 2718 6648 2718 Size: 3930 3055 BspHI T'CATG_A Cuts at: 2869 4874 6604 2869 Size: 2005 1730 3250 Fragments arranged by size: 3250 2005 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4340 844 Size: 3496 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2952 4872 1394 Size: 429 1129 1920 3507 Fragments arranged by size: 3507 1920 1129 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2740 2969 3383 5480 5654 65 Size: 1183 1492 229 414 2097 174 1396 Fragments arranged by size: 2097 1492 1396 1183 414 229 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2616 5629 5816 1700 Size: 110 806 3013 187 2869 Fragments arranged by size: 3013 2869 806 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6985 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6985 BssSI C'ACGA_G Cuts at: 5045 6057 5045 Size: 1012 5973 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6985 BstBI TT'CG_AA Cuts at: 5789 5789 Size: 6985 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6985 BstYI r'GATC_y Cuts at: 1972 3097 3278 4177 4315 4682 5067 5084 6536 Size: 1125 181 899 138 367 385 17 1452 Cuts at: 6536 6622 6634 6980 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1125 899 385 367 346 181 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3050 3050 Size: 6985 BtgI C'CryG_G Cuts at: 513 1065 3375 513 Size: 552 2310 4123 Fragments arranged by size: 4123 2310 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6246 Fragments arranged by size: 6246 683 56 BtsI GCAGTG_nn' Cuts at: 2098 3701 4501 5295 5315 2098 Size: 1603 800 794 20 3768 Fragments arranged by size: 3768 1603 800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6985 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6950 DraI TTT'AAA Cuts at: 4289 4289 Size: 6985 DraIII CAC_nnn'GTG Cuts at: 2912 2912 Size: 6985 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5992 809 Size: 507 4676 1802 Fragments arranged by size: 4676 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4432 5321 6950 Size: 54 1425 50 275 18 2602 889 1629 Cuts at: 6950 8 Size: 43 Fragments arranged by size: 2602 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4432 1537 Size: 275 2620 4090 Fragments arranged by size: 4090 2620 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2635 3370 4915 1353 Size: 660 622 735 1545 3423 Fragments arranged by size: 3423 1545 735 660 622 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5572 5946 6092 387 Size: 1064 138 3983 374 146 1280 Fragments arranged by size: 3983 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2716 2716 Size: 6985 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2712 3303 3402 3510 3798 5048 5645 Size: 393 1080 591 99 108 288 1250 597 Cuts at: 5645 6432 6700 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1250 1080 787 597 591 393 288 268 108 99 EcoRI G'AATT_C Cuts at: 4294 4672 4294 Size: 378 6607 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2793 2793 Size: 6985 FspI TGC'GCA Cuts at: 5491 5491 Size: 6985 HaeII r_GCGC'y Cuts at: 1678 2762 6132 1678 Size: 1084 3370 2531 Fragments arranged by size: 3370 2531 1084 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 2345 2377 3257 Size: 32 135 32 481 32 260 32 880 Cuts at: 3257 3289 3571 3603 3776 3808 4133 4165 5626 Size: 32 282 32 173 32 325 32 1461 Cuts at: 5626 5658 5700 5732 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1461 880 481 325 282 260 173 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3442 4329 4575 669 Size: 988 281 939 565 887 246 3079 Fragments arranged by size: 3079 988 939 887 565 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6985 HpaI GTT'AAC Cuts at: 4575 4575 Size: 6985 KpnI G_GTAC'C Cuts at: 2949 4310 2949 Size: 1361 5624 MfeI C'AATT_G Cuts at: 4074 4584 4074 Size: 510 6475 MluI A'CGCG_T Cuts at: 6861 6861 Size: 6985 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4213 6099 6283 1305 Size: 2908 1886 184 2007 Fragments arranged by size: 2908 2007 1886 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5163 Fragments arranged by size: 5163 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2733 3136 3189 4943 5302 5461 518 Size: 648 1567 403 53 1754 359 159 2042 Fragments arranged by size: 2042 1754 1567 648 403 359 159 53 MspA1I CmG'CkG Cuts at: 1606 2927 3608 4401 5078 6226 6471 1606 Size: 1321 681 793 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 793 681 677 245 NcoI C'CATG_G Cuts at: 513 1065 3375 513 Size: 552 2310 4123 Fragments arranged by size: 4123 2310 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6985 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6985 NotI GC'GGCC_GC Cuts at: 4432 4432 Size: 6985 NspI r_CATG'y Cuts at: 1132 1229 2656 3323 4343 5888 1132 Size: 97 1427 667 1020 1545 2229 Fragments arranged by size: 2229 1545 1427 1020 667 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6985 PciI A'CATG_T Cuts at: 5884 5884 Size: 6985 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6985 PmeI GTTT'AAAC Cuts at: 4289 4289 Size: 6985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5054 5086 6567 6599 5054 Size: 32 1481 32 5440 Fragments arranged by size: 5440 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6985 PsiI TTA'TAA Cuts at: 4555 4555 Size: 6985 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3112 3144 3112 Size: 32 6953 PstI C_TGCA'G Cuts at: 830 4337 830 Size: 3507 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6985 PvuII CAG'CTG Cuts at: 2927 3608 2927 Size: 681 6304 SacI G_AGCT'C Cuts at: 2718 2718 Size: 6985 SalI G'TCGA_C Cuts at: 1936 4327 1936 Size: 2391 4594 SbfI CC_TGCA'GG Cuts at: 4337 4337 Size: 6985 ScaI AGT'ACT Cuts at: 5233 6945 5233 Size: 1712 5273 SfcI C'TryA_G Cuts at: 826 1045 2544 2590 2598 3476 3509 4050 4333 Size: 219 1499 46 8 878 33 541 283 Cuts at: 4333 5468 6149 6340 826 Size: 1135 681 191 1471 Fragments arranged by size: 1499 1471 1135 878 681 541 283 219 191 46 33 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6985 SmaI CCC'GGG Cuts at: 1872 4312 1872 Size: 2440 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 2708 3553 5089 5990 6252 Size: 197 926 576 189 845 1536 901 262 Cuts at: 6252 820 Size: 1553 Fragments arranged by size: 1553 1536 926 901 845 576 262 197 189 SnaBI TAC'GTA Cuts at: 493 4776 493 Size: 4283 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6985 SphI G_CATG'C Cuts at: 1132 4343 1132 Size: 3211 3774 SspI AAT'ATT Cuts at: 5 52 2506 4909 5 Size: 47 2454 2403 2081 Fragments arranged by size: 2454 2403 2081 47 StuI AGG'CCT Cuts at: 1517 3564 1517 Size: 2047 4938 StyI C'CwwG_G Cuts at: 513 1065 2367 2475 3024 3375 3559 4227 4424 Size: 552 1302 108 549 351 184 668 197 Cuts at: 4424 513 Size: 3074 Fragments arranged by size: 3074 1302 668 552 549 351 197 184 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3422 5012 5029 5182 5367 5812 325 Size: 1255 1842 1590 17 153 185 445 1498 Fragments arranged by size: 1842 1590 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5231 6943 96 Size: 274 80 33 51 1443 3254 1712 138 Fragments arranged by size: 3254 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2989 3021 3508 3540 6945 6977 2989 Size: 32 487 32 3405 32 2997 Fragments arranged by size: 3405 2997 487 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2900 1316 Size: 1584 5401 XbaI T'CTAG_A Cuts at: 4321 4321 Size: 6985 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6985 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6985 XmaI C'CCGG_G Cuts at: 1870 4310 1870 Size: 2440 4545 XmnI GAAnn'nnTTC Cuts at: 2865 5114 2865 Size: 2249 4736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4793 276 Size: 53 83 186 936 3259 2468 Fragments arranged by size: 3259 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI