(Linear) (Six-base) MAPSORT of: KIBB6251.seq Check: 615 from: 1 to: 2280 KIBB6251.orf With 182 enzymes: SgfI * July 3, 2012 16:17 .. Acc65I G'GTAC_C Cuts at: 0 940 2280 Size: 940 1340 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 707 1397 2280 Size: 707 690 883 Fragments arranged by size: 883 707 690 AflII C'TTAA_G Cuts at: 0 514 2280 Size: 514 1766 AhdI GACnn_n'nnGTC Cuts at: 0 1580 2280 Size: 1580 700 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1177 1209 1265 1297 2280 Size: 1177 32 56 32 983 Fragments arranged by size: 1177 983 56 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1276 1606 2280 Size: 1276 330 674 Fragments arranged by size: 1276 674 330 ApoI r'AATT_y Cuts at: 0 1844 1900 2280 Size: 1844 56 380 Fragments arranged by size: 1844 380 56 BanI G'GyrC_C Cuts at: 0 788 940 1525 2280 Size: 788 152 585 755 Fragments arranged by size: 788 755 585 152 BanII G_rGCy'C Cuts at: 0 713 2280 Size: 713 1567 BbvCI CC'TCA_GC Cuts at: 0 1103 2280 Size: 1103 1177 BciVI GTATCCnnnnn_n' Cuts at: 0 798 1905 2280 Size: 798 1107 375 Fragments arranged by size: 1107 798 375 BclI T'GATC_A Cuts at: 0 180 243 2061 2280 Size: 180 63 1818 219 Fragments arranged by size: 1818 219 180 63 BmrI ACTGGGnnnn_n' Cuts at: 0 744 1098 2280 Size: 744 354 1182 Fragments arranged by size: 1182 744 354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1298 1505 1793 2280 Size: 1298 207 288 487 Fragments arranged by size: 1298 487 288 207 Bpu10I CC'TnA_GC Cuts at: 0 1103 1802 2280 Size: 1103 699 478 Fragments arranged by size: 1103 699 478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 1533 2280 Size: 688 845 747 Fragments arranged by size: 845 747 688 BsaHI Gr'CG_yC Cuts at: 0 1814 2280 Size: 1814 466 BsaWI w'CCGG_w Cuts at: 0 713 2280 Size: 713 1567 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1503 1533 2280 Size: 1503 30 747 Fragments arranged by size: 1503 747 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1330 1708 2280 Size: 1330 378 572 Fragments arranged by size: 1330 572 378 BsiHKAI G_wGCw'C Cuts at: 0 7 713 2280 Size: 7 706 1567 Fragments arranged by size: 1567 706 7 Bsp1286I G_dGCh'C Cuts at: 0 7 713 2280 Size: 7 706 1567 Fragments arranged by size: 1567 706 7 BspEI T'CCGG_A Cuts at: 0 713 2280 Size: 713 1567 BspHI T'CATG_A Cuts at: 0 864 2280 Size: 864 1416 BsrBI CCG'CTC Cuts at: 0 947 2280 Size: 947 1333 BsrDI GCAATG_nn' Cuts at: 0 735 964 1378 2280 Size: 735 229 414 902 Fragments arranged by size: 902 735 414 229 BsrFI r'CCGG_y Cuts at: 0 611 2280 Size: 611 1669 BstYI r'GATC_y Cuts at: 0 1092 1273 2172 2280 Size: 1092 181 899 108 Fragments arranged by size: 1092 899 181 108 Bsu36I CC'TnA_GG Cuts at: 0 1045 2280 Size: 1045 1235 BtgI C'CryG_G Cuts at: 0 1370 2280 Size: 1370 910 BtsI GCAGTG_nn' Cuts at: 0 93 1696 2280 Size: 93 1603 584 Fragments arranged by size: 1603 584 93 DraIII CAC_nnn'GTG Cuts at: 0 907 2280 Size: 907 1373 EarI CTCTTCn'nnn_ Cuts at: 0 8 630 1365 2280 Size: 8 622 735 915 Fragments arranged by size: 915 735 622 8 EcoICRI GAG'CTC Cuts at: 0 711 2280 Size: 711 1569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 707 1298 1397 1505 1793 2280 Size: 707 591 99 108 288 487 Fragments arranged by size: 707 591 487 288 108 99 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 788 2280 Size: 788 1492 HaeII r_GCGC'y Cuts at: 0 757 2280 Size: 757 1523 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 48 80 340 372 1252 1284 1566 1598 Size: 48 32 260 32 880 32 282 32 Cuts at: 1598 1771 1803 2128 2160 2280 Size: 173 32 325 32 120 Fragments arranged by size: 880 325 282 260 173 120 48 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 872 1437 2280 Size: 872 565 843 Fragments arranged by size: 872 843 565 KpnI G_GTAC'C Cuts at: 0 944 2280 Size: 944 1336 MfeI C'AATT_G Cuts at: 0 2069 2280 Size: 2069 211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2208 2280 Size: 2208 72 MslI CAynn'nnrTG Cuts at: 0 728 1131 1184 2280 Size: 728 403 53 1096 Fragments arranged by size: 1096 728 403 53 MspA1I CmG'CkG Cuts at: 0 922 1603 2280 Size: 922 681 677 Fragments arranged by size: 922 681 677 NcoI C'CATG_G Cuts at: 0 1370 2280 Size: 1370 910 NspI r_CATG'y Cuts at: 0 651 1318 2280 Size: 651 667 962 Fragments arranged by size: 962 667 651 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1107 1139 2280 Size: 1107 32 1141 Fragments arranged by size: 1141 1107 32 PvuII CAG'CTG Cuts at: 0 922 1603 2280 Size: 922 681 677 Fragments arranged by size: 922 681 677 SacI G_AGCT'C Cuts at: 0 713 2280 Size: 713 1567 SfcI C'TryA_G Cuts at: 0 539 585 593 1471 1504 2045 2280 Size: 539 46 8 878 33 541 235 Fragments arranged by size: 878 541 539 235 46 33 8 SmlI C'TyrA_G Cuts at: 0 514 703 1548 2280 Size: 514 189 845 732 Fragments arranged by size: 845 732 514 189 SspI AAT'ATT Cuts at: 0 501 2280 Size: 501 1779 StuI AGG'CCT Cuts at: 0 1559 2280 Size: 1559 721 StyI C'CwwG_G Cuts at: 0 362 470 1019 1370 1554 2222 2280 Size: 362 108 549 351 184 668 58 Fragments arranged by size: 668 549 362 351 184 108 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1417 2280 Size: 1417 863 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 984 1016 1503 1535 2280 Size: 984 32 487 32 745 Fragments arranged by size: 984 745 487 32 32 Tth111I GACn'n_nGTC Cuts at: 0 895 2280 Size: 895 1385 XmnI GAAnn'nnTTC Cuts at: 0 860 2280 Size: 860 1420 Enzymes that do cut: Acc65I AcuI AflII AhdI AloI AlwNI ApoI BanI BanII BbvCI BciVI BclI BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstYI Bsu36I BtgI BtsI DraIII EarI EcoICRI Eco57MI FalI HaeII Hin4I HincII KpnI MfeI MmeI MslI MspA1I NcoI NspI PsrI PvuII SacI SfcI SmlI SspI StuI StyI TaqII TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflIII AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI XbaI XcmI XhoI XmaI ZraI