################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII AvrII BbrPI BcgI BfrI BlnI BsaAI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoT22I FseI FspI HindIII HpaI KspI MfeI MunI NdeI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 2728 GACGTC GACGT^C Acc65I 340 GGTACC G^GTACC AccI 2788 GTCGAC GT^[AC][GT]AC AccIII 1623 TCCGGA T^CCGGA AcsI 1397 GAATTC [GA]^AATT[CT] Alw44I 1954 GTGCAC G^TGCAC ApaLI 1954 GTGCAC G^TGCAC ApoI 1397 GAATTC [GA]^AATT[CT] Asp700I 1397 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 340 GGTACC G^GTACC AspI 594 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 896 TGGCCA TGG^CCA BciVI 770 GTATCC GTATCC(6/5) BclI 35 TGATCA T^GATCA BglII 1449 AGATCT A^GATCT BlpI 123 GCTGAGC GC^T[ACGT]AGC Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC BsaBI 2226 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1623 TCCGGA [AT]^CCGG[AT] BseAI 1623 TCCGGA T^CCGGA BspEI 1623 TCCGGA T^CCGGA BspHI 38 TCATGA T^CATGA BspLU11I 2294 ACATGT A^CATGT BsrDI 612 GCAATG GCAATG(2/0) BssSI 2611 CACGAG CACGAG(-5/-1) BstAPI 405 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2573 TTCGAA TT^CGAA BstEII 2496 GGTCACC G^GT[ACGT]ACC BstPI 2496 GGTCACC G^GT[ACGT]ACC CelII 123 GCTGAGC GC^T[ACGT]AGC DrdI 2799 GACCGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2496 GGTCACC G^GT[ACGT]ACC EcoRI 1397 GAATTC G^AATTC EcoRV 1115 GATATC GAT^ATC FbaI 35 TGATCA T^GATCA HincII 2788 GTCGAC GT[CT]^[GA]AC HindII 2788 GTCGAC GT[CT]^[GA]AC KpnI 340 GGTACC GGTAC^C MamI 2226 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1552 ACGCGT A^CGCGT MluNI 896 TGGCCA TGG^CCA MroI 1623 TCCGGA T^CCGGA MscI 896 TGGCCA TGG^CCA MseI 889 TTAA T^TAA NaeI 233 GCCGGC GCC^GGC NgoMIV 233 GCCGGC G^CCGGC NheI 883 GCTAGC G^CTAGC NspV 2573 TTCGAA TT^CGAA PflFI 594 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 38 TCATGA T^CATGA SalI 2788 GTCGAC G^TCGAC SapI 60 GAAGAGC GCTCTTC(1/4) SfuI 2573 TTCGAA TT^CGAA SmlI 988 CTTGAG C^T[CT][GA]AG SphI 1130 GCATGC GCATG^C Tru9I 889 TTAA T^TAA Tth111I 594 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2583 TCTAGA T^CTAGA XmnI 1397 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 1552 ACGCGT A^C[GA][CT]GT 2294 ACATGT AlwNI 1033 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 2713 CAGACCCTG Aor51HI 2081 AGCGCT AGC^GCT 2162 AGCGCT BamHI 2000 GGATCC G^GATCC 2051 GGATCC BbeI 157 GGCGCC GGCGC^C 571 GGCGCC BbvCI 1361 CCTCAGC CCTCAGC(-5/-2) 2112 GCTGAGG BsaI 2392 GAGACC GGTCTC(1/5) 1961 GGTCTC BsgI 412 GTGCAG GTGCAG(16/14) 1492 GTGCAG BsiEI 231 CGGCCG CG[GA][CT]^CG 705 CGGCCG BsmI 689 GAATGC GAATGC(1/-1) 1412 GAATGC BsrBI 63 GAGCGG CCGCTC(-3/-3) 228 GAGCGG EagI 231 CGGCCG C^GGCCG 705 CGGCCG EclXI 231 CGGCCG C^GGCCG 705 CGGCCG Eco47III 2081 AGCGCT AGC^GCT 2162 AGCGCT Eco52I 231 CGGCCG C^GGCCG 705 CGGCCG KasI 157 GGCGCC G^GCGCC 571 GGCGCC MaeII 410 ACGT A^CGT 2729 ACGT NarI 157 GGCGCC GG^CGCC 571 GGCGCC NcoI 1526 CCATGG C^CATGG 2136 CCATGG PvuII 2005 CAGCTG CAG^CTG 2036 CAGCTG SacI 462 GAGCTC GAGCT^C 2254 GAGCTC SfoI 157 GGCGCC GGC^GCC 571 GGCGCC StuI 1667 AGGCCT AGG^CCT 2504 AGGCCT TfiI 2231 GAATC G^A[AT]TC 2435 GAATC Tsp509I 813 AATT ^AATT 1398 AATT XcmI 2405 CCACGGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2610 CCACGAGGTCATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AocI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI