Restriction map for
pFN21AB8513
(Circular) (Six-base) MAPSORT of: pFN21AB8513.seq Check: 5459 from: 1 to: 7534
pFN21AB8513 7534 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2320 2410 2671 2320
Size: 90 261 7183
Fragments arranged by size:
7183 261 90
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4737 5344 278
Size: 53 83 186 936 3201 607 2468
Fragments arranged by size:
3201 2468 936 607 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4794 4877 1937
Size: 2857 83 4594
Fragments arranged by size:
4594 2857 83
Acc65I G'GTAC_C
Cuts at: 2345 4855 2345
Size: 2510 5024
AclI AA'CG_TT
Cuts at: 1563 5661 6034 1563
Size: 4098 373 3063
Fragments arranged by size:
4098 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2616 3177 4082 4498 4589 5597 6981 7249
Size: 984 561 905 416 91 1008 1384 268
Cuts at: 7249 1632
Size: 1917
Fragments arranged by size:
1917 1384 1008 984 905 561 416 268
91
AfeI AGC'GCT
Cuts at: 4088 4169 4088
Size: 81 7453
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7337
AflIII A'CryG_T
Cuts at: 1203 3557 4299 6433 7410 1203
Size: 2354 742 2134 977 1327
Fragments arranged by size:
2354 2134 1327 977 742
AgeI A'CCGG_T
Cuts at: 6365 6365
Size: 7534
AhdI GACnn_n'nnGTC
Cuts at: 6263 6263
Size: 7534
AleI CACnn'nnGTG
Cuts at: 1166 2909 3569 1166
Size: 1743 660 5131
Fragments arranged by size:
5131 1743 660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3587 1184
Size: 2403 5131
AlwNI CAG_nnn'CTG
Cuts at: 3043 4723 5277 6849 3043
Size: 1680 554 1572 3728
Fragments arranged by size:
3728 1680 1572 554
ApaI G_GGCC'C
Cuts at: 2088 2664 3084 3247 3286 2088
Size: 576 420 163 39 6336
Fragments arranged by size:
6336 576 420 163 39
ApaLI G'TGCA_C
Cuts at: 3959 5591 6747 3959
Size: 1632 1156 4746
Fragments arranged by size:
4746 1632 1156
ApoI r'AATT_y
Cuts at: 1493 3402 4843 5069 5221 7330 1493
Size: 1909 1441 226 152 2109 1697
Fragments arranged by size:
2109 1909 1697 1441 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6088 160
Size: 872 5056 1606
Fragments arranged by size:
5056 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7534
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2113 2153 2201 2219 2842 3762
Size: 73 137 33 40 48 18 623 920
Cuts at: 3762 4328 4774 4859 1870
Size: 566 446 85 4545
Fragments arranged by size:
4545 920 623 566 446 137 85 73
48 40 33 18
BamHI G'GATC_C
Cuts at: 4005 4056 4864 5231 4005
Size: 51 808 367 6308
Fragments arranged by size:
6308 808 367 51
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2345 2576 3527 3719
Size: 325 217 627 375 183 231 951 192
Cuts at: 3719 4457 4855 6310 618
Size: 738 398 1455 1842
Fragments arranged by size:
1842 1455 951 738 627 398 375 325
231 217 192 183
BanII G_rGCy'C
Cuts at: 1391 2088 2135 2211 2219 2471 2512 2664 2834
Size: 697 47 76 8 252 41 152 170
Cuts at: 2834 2986 3084 3146 3247 3286 4263 4782 4853
Size: 152 98 62 101 39 977 519 71
Cuts at: 4853 1391
Size: 4072
Fragments arranged by size:
4072 977 697 519 252 170 152 152
101 98 76 71 62 47 41 39
8
BbeI G_GCGC'C
Cuts at: 2166 2580 2166
Size: 414 7120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3840 4458 4487 928
Size: 971 1941 618 29 3975
Fragments arranged by size:
3975 1941 971 618 29
BbvCI CC'TCA_GC
Cuts at: 3367 4118 3367
Size: 751 6783
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5725 5759 1903
Size: 34 3788 34 3678
Fragments arranged by size:
3788 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2786 5426 6636 2786
Size: 2640 1210 3684
Fragments arranged by size:
3684 2640 1210
BclI T'GATC_A
Cuts at: 1548 2040 1548
Size: 492 7042
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2223 3548 3765
Size: 107 122 71 631 605 551 1325 217
Cuts at: 3765 6145 136
Size: 2380 1525
Fragments arranged by size:
2380 1525 1325 631 605 551 217 122
107 71
BglII A'GATC_T
Cuts at: 3454 7529 3454
Size: 4075 3459
BlpI GC'TnA_GC
Cuts at: 2129 4951 2129
Size: 2822 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2531 2664 3084 3247 3286 3476 3530
Size: 298 443 133 420 163 39 190 54
Cuts at: 3530 3722 3963 5595 6751 1790
Size: 192 241 1632 1156 2573
Fragments arranged by size:
2573 1632 1156 443 420 298 241 192
190 163 133 54 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2082 2943 5265
Size: 416 53 83 399 24 665 861 2322
Cuts at: 5265 6223 442
Size: 958 1753
Fragments arranged by size:
2322 1753 958 861 665 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2892 1056
Size: 1836 5698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3868 3868
Size: 7534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3443 3496 3838 4271 6194 1239
Size: 2204 53 342 433 1923 2579
Fragments arranged by size:
2579 2204 1923 433 342 53
Bpu10I CC'TnA_GC
Cuts at: 2739 3221 3367 3864 4118 2739
Size: 482 146 497 254 6155
Fragments arranged by size:
6155 497 482 254 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3014 5659 6524 6822 3014
Size: 2645 865 298 3726
Fragments arranged by size:
3726 2645 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3972 4391 6197 882
Size: 617 2473 419 1806 2219
Fragments arranged by size:
2473 2219 1806 617 419
BsaAI yAC'GTr
Cuts at: 493 1204 5325 493
Size: 711 4121 2702
Fragments arranged by size:
4121 2702 711
BsaBI GATnn'nnATC
Cuts at: 4235 7528 4235
Size: 3293 4241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2163 2577 4734
Size: 53 83 186 936 569 61 414 2157
Cuts at: 4734 5341 5723 6358 275
Size: 607 382 635 1451
Fragments arranged by size:
2157 1451 936 635 607 569 414 382
186 83 61 53
BsaWI w'CCGG_w
Cuts at: 3628 5967 6365 6639 6786 7197 3628
Size: 2339 398 274 147 411 3965
Fragments arranged by size:
3965 2339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2971 3001 3317 3347 1178
Size: 30 165 30 1568 30 316 30 5365
Fragments arranged by size:
5365 1568 316 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 2219 2384 2665 3191 3281 3758 3796
Size: 945 88 165 281 526 90 477 38
Cuts at: 3796 3811 3857 4268 4271 4358 4628 1186
Size: 15 46 411 3 87 270 4092
Fragments arranged by size:
4092 945 526 477 411 281 270 165
90 88 87 46 38 15 3
BseYI C'CCAG_C
Cuts at: 2182 2663 3061 3343 3683 4283 6737 7233 2182
Size: 481 398 282 340 600 2454 496 2483
Fragments arranged by size:
2483 2454 600 496 481 398 340 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2438 3518 1163
Size: 573 702 1080 5179
Fragments arranged by size:
5179 1080 702 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2239 2713 4984 5745 6773 1540
Size: 275 186 238 474 2271 761 1028 2301
Fragments arranged by size:
2301 2271 1028 761 474 275 238 186
BsiHKAI G_wGCw'C
Cuts at: 2471 3963 4263 5595 5680 6751 2471
Size: 1492 300 1332 85 1071 3254
Fragments arranged by size:
3254 1492 1332 1071 300 85
BsmI GAATG_Cn'
Cuts at: 2700 3423 5045 5138 2700
Size: 723 1622 93 5096
Fragments arranged by size:
5096 1622 723 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2093 3173 3740 2093
Size: 1080 567 5887
Fragments arranged by size:
5887 1080 567
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2135 2211 2219 2471 2512 2531
Size: 399 298 47 76 8 252 41 19
Cuts at: 2531 2664 2834 2986 3084 3146 3247 3286 3476
Size: 133 170 152 98 62 101 39 190
Cuts at: 3476 3530 3722 3963 4263 4782 4853 5595 5680
Size: 54 192 241 300 519 71 742 85
Cuts at: 5680 6751 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 519 399 300 298 252
241 192 190 170 152 133 101 98
85 76 71 62 54 47 41 39
19 8
BspEI T'CCGG_A
Cuts at: 3628 7197 3628
Size: 3569 3965
BspHI T'CATG_A
Cuts at: 2043 5423 7153 2043
Size: 3380 1730 2424
Fragments arranged by size:
3380 2424 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 2410 2671 3009 3703 3780 4141 4197
Size: 1476 90 261 338 694 77 361 56
Cuts at: 4197 4489 4889 844
Size: 292 400 3489
Fragments arranged by size:
3489 1476 694 400 361 338 292 261
90 77 56
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 2235 5421 1394
Size: 429 247 165 3186 3507
Fragments arranged by size:
3507 3186 429 247 165
BsrDI GCAATG_nn'
Cuts at: 65 1248 2624 6029 6203 65
Size: 1183 1376 3405 174 1396
Fragments arranged by size:
3405 1396 1376 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 2322 2342 3542 4098 4563 6178
Size: 110 428 84 20 1200 556 465 1615
Cuts at: 6178 6365 1700
Size: 187 2869
Fragments arranged by size:
2869 1615 1200 556 465 428 187 110
84 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7534
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7534
BssSI C'ACGA_G
Cuts at: 4616 5594 6606 4616
Size: 978 1012 5544
Fragments arranged by size:
5544 1012 978
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2416 794
Size: 1622 5912
BstBI TT'CG_AA
Cuts at: 4579 6338 4579
Size: 1759 5775
BstEII G'GTnAC_C
Cuts at: 1184 4501 1184
Size: 3317 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2911 3247 3731 2812
Size: 99 336 484 6615
Fragments arranged by size:
6615 484 336 99
BstYI r'GATC_y
Cuts at: 1972 2391 2953 3454 4005 4056 4864 5231 5616
Size: 419 562 501 551 51 808 367 385
Cuts at: 5616 5633 7085 7171 7183 7529 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 808 562 551 501 419 385
367 346 86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 3264 3665 3779 4710 3264
Size: 401 114 931 6088
Fragments arranged by size:
6088 931 401 114
BtgI C'CryG_G
Cuts at: 513 1065 2004 2904 3288 3531 3810 4141 4376
Size: 552 939 900 384 243 279 331 235
Cuts at: 4376 4410 513
Size: 34 3637
Fragments arranged by size:
3637 939 900 552 384 331 279 243
235 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3102 487
Size: 683 56 1876 4919
Fragments arranged by size:
4919 1876 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2272 3055 4394 5050 5844 5864 2130
Size: 142 783 1339 656 794 20 3800
Fragments arranged by size:
3800 1339 794 783 656 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7499
DraI TTT'AAA
Cuts at: 4838 4838
Size: 7534
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 6541 809
Size: 507 3494 1731 1802
Fragments arranged by size:
3494 1802 1731 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 2710 2901
Size: 54 1425 50 275 18 406 474 191
Cuts at: 2901 4194 4413 4981 5870 7499 8
Size: 1293 219 568 889 1629 43
Fragments arranged by size:
1629 1425 1293 889 568 474 406 275
219 191 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2236 2710 4981 1537
Size: 275 424 474 2271 4090
Fragments arranged by size:
4090 2271 474 424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2180 2709 3799 4646 5464 1353
Size: 707 120 529 1090 847 818 3423
Fragments arranged by size:
3423 1090 847 818 707 529 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2245 2461 6121 6495 6641 387
Size: 1064 138 656 216 3660 374 146 1280
Fragments arranged by size:
3660 1280 1064 656 374 216 146 138
EcoICRI GAG'CTC
Cuts at: 2469 4261 2469
Size: 1792 5742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2616 3177 3443 3496 3838 4082 4271
Size: 393 984 561 266 53 342 244 189
Cuts at: 4271 4498 4589 5597 6194 6981 7249 1239
Size: 227 91 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 984 787 597 561 393 342
268 266 244 227 189 91 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 3668 4253 4292 4709 2570
Size: 1098 585 39 417 5395
Fragments arranged by size:
5395 1098 585 417 39
EcoO109I rG'GnC_Cy
Cuts at: 2084 2563 2660 2998 3016 3081 3243 3244 3260
Size: 479 97 338 18 65 162 1 16
Cuts at: 3260 3282 3283 3333 3735 4122 4242 4333 2084
Size: 22 1 50 402 387 120 91 5285
Fragments arranged by size:
5285 479 402 387 338 162 120 97
91 65 50 22 18 16 1 1
EcoRI G'AATT_C
Cuts at: 3402 4843 5221 3402
Size: 1441 378 5715
Fragments arranged by size:
5715 1441 378
EcoRV GAT'ATC
Cuts at: 3122 3122
Size: 7534
FspI TGC'GCA
Cuts at: 6040 6040
Size: 7534
HaeII r_GCGC'y
Cuts at: 1678 2055 2166 2486 2580 4090 4171 6681 1678
Size: 377 111 320 94 1510 81 2510 2531
Fragments arranged by size:
2531 2510 1510 377 320 111 94 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 3317 3349 3869
Size: 32 135 32 608 32 1105 32 520
Cuts at: 3869 3901 4190 4222 4540 4572 6175 6207 6249
Size: 32 289 32 318 32 1603 32 42
Cuts at: 6249 6281 1373
Size: 32 2626
Fragments arranged by size:
2626 1603 1105 608 520 318 289 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4795 4878 5124 669
Size: 988 281 2857 83 246 3079
Fragments arranged by size:
3079 2857 988 281 246 83
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7534
HpaI GTT'AAC
Cuts at: 5124 5124
Size: 7534
KasI G'GCGC_C
Cuts at: 2162 2576 2162
Size: 414 7120
KpnI G_GTAC'C
Cuts at: 2349 4859 2349
Size: 2510 5024
MfeI C'AATT_G
Cuts at: 5133 5133
Size: 7534
MluI A'CGCG_T
Cuts at: 3557 7410 3557
Size: 3853 3681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3339 6648 6832 1305
Size: 2034 3309 184 2007
Fragments arranged by size:
3309 2034 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 10
Size: 54 1425 343 1071 4641
Fragments arranged by size:
4641 1425 1071 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2810 2909 3569 4146 5492 5851
Size: 648 843 801 99 660 577 1346 359
Cuts at: 5851 6010 518
Size: 159 2042
Fragments arranged by size:
2042 1346 843 801 660 648 577 359
159 99
MspA1I CmG'CkG
Cuts at: 1606 2262 2274 2793 3601 4012 4043 4360 4950
Size: 656 12 519 808 411 31 317 590
Cuts at: 4950 5627 6775 7020 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 808 677 656 590 519 411
317 245 31 12
NaeI GCC'GGC
Cuts at: 2240 2240
Size: 7534
NarI GG'CG_CC
Cuts at: 2163 2577 2163
Size: 414 7120
NcoI C'CATG_G
Cuts at: 513 1065 2004 3531 4141 513
Size: 552 939 1527 610 3906
Fragments arranged by size:
3906 1527 939 610 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7534
NgoMIV G'CCGG_C
Cuts at: 2238 2238
Size: 7534
NheI G'CTAG_C
Cuts at: 1052 2888 1052
Size: 1836 5698
NotI GC'GGCC_GC
Cuts at: 4981 4981
Size: 7534
NspI r_CATG'y
Cuts at: 1132 1229 2443 3139 4303 4387 4892 6437 1132
Size: 97 1214 696 1164 84 505 1545 2229
Fragments arranged by size:
2229 1545 1214 1164 696 505 97 84
PasI CC'CwG_GG
Cuts at: 1348 3101 3240 4420 1348
Size: 1753 139 1180 4462
Fragments arranged by size:
4462 1753 1180 139
PciI A'CATG_T
Cuts at: 4299 6433 4299
Size: 2134 5400
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 3519 4674 1108
Size: 1326 1085 1155 3968
Fragments arranged by size:
3968 1326 1155 1085
PfoI T'CCnGG_A
Cuts at: 2645 2645
Size: 7534
PmeI GTTT'AAAC
Cuts at: 4838 4838
Size: 7534
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5603 5635 7116 7148 5603
Size: 32 1481 32 5989
Fragments arranged by size:
5989 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2563 2998 3333 4242 2563
Size: 435 335 909 5855
Fragments arranged by size:
5855 909 435 335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7534
PsiI TTA'TAA
Cuts at: 5104 5104
Size: 7534
PspOMI G'GGCC_C
Cuts at: 2084 2660 3080 3243 3282 2084
Size: 576 420 163 39 6336
Fragments arranged by size:
6336 576 420 163 39
PstI C_TGCA'G
Cuts at: 830 3006 3680 4728 4886 830
Size: 2176 674 1048 158 3478
Fragments arranged by size:
3478 2176 1048 674 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7534
PvuII CAG'CTG
Cuts at: 4012 4043 4012
Size: 31 7503
SacI G_AGCT'C
Cuts at: 2471 4263 2471
Size: 1792 5742
SalI G'TCGA_C
Cuts at: 1936 4793 4876 1936
Size: 2857 83 4594
Fragments arranged by size:
4594 2857 83
SanDI GG'GwC_CC
Cuts at: 3333 3333
Size: 7534
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 7534
SbfI CC_TGCA'GG
Cuts at: 4886 4886
Size: 7534
ScaI AGT'ACT
Cuts at: 5782 7494 5782
Size: 1712 5822
SfcI C'TryA_G
Cuts at: 826 1045 2724 3002 3676 4724 4882 6017 6698
Size: 219 1679 278 674 1048 158 1135 681
Cuts at: 6698 6889 826
Size: 191 1471
Fragments arranged by size:
1679 1471 1135 1048 681 674 278 219
191 158
SfoI GGC'GCC
Cuts at: 2164 2578 2164
Size: 414 7120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7534
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7534
SmaI CCC'GGG
Cuts at: 1872 2082 2155 2221 2844 4861 1872
Size: 210 73 66 623 2017 4545
Fragments arranged by size:
4545 2017 623 210 73 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2993 5638 6539 6801 820
Size: 197 926 1050 2645 901 262 1553
Fragments arranged by size:
2645 1553 1050 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5325 493
Size: 4832 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7534
SphI G_CATG'C
Cuts at: 1132 3139 4892 1132
Size: 2007 1753 3774
Fragments arranged by size:
3774 2007 1753
SspI AAT'ATT
Cuts at: 5 52 5458 5
Size: 47 5406 2081
Fragments arranged by size:
5406 2081 47
StuI AGG'CCT
Cuts at: 1517 3674 4511 1517
Size: 2157 837 4540
Fragments arranged by size:
4540 2157 837
StyI C'CwwG_G
Cuts at: 513 1065 2004 3531 3738 4141 4973 513
Size: 552 939 1527 207 403 832 3074
Fragments arranged by size:
3074 1527 939 832 552 403 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 3482 3737 4820 5561 5578 5731
Size: 1255 1238 664 255 1083 741 17 153
Cuts at: 5731 5916 6361 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1238 1083 741 664 445 255
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2013 5780 7492
Size: 274 80 33 51 1443 36 3767 1712
Cuts at: 7492 96
Size: 138
Fragments arranged by size:
3767 1712 1443 274 138 80 51 36
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2971 3003 7494 7526 2971
Size: 32 4491 32 2979
Fragments arranged by size:
4491 2979 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2602 1316
Size: 1286 6248
XbaI T'CTAG_A
Cuts at: 4588 4870 4588
Size: 282 7252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4417 4622 4417
Size: 205 7329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7534
XmaI C'CCGG_G
Cuts at: 1870 2080 2153 2219 2842 4859 1870
Size: 210 73 66 623 2017 4545
Fragments arranged by size:
4545 2017 623 210 73 66
XmnI GAAnn'nnTTC
Cuts at: 3406 5663 3406
Size: 2257 5277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4735 5342 276
Size: 53 83 186 936 3201 607 2468
Fragments arranged by size:
3201 2468 936 607 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SexAI SfiI SrfI SwaI