(Linear) (Six-base) MAPSORT of: KIBB8513.seq Check: 8068 from: 1 to: 2829 KIBB8513.orf With 182 enzymes: SgfI * July 3, 2012 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 315 405 666 2829 Size: 315 90 261 2163 Fragments arranged by size: 2163 315 261 90 AatII G_ACGT'C Cuts at: 0 2732 2829 Size: 2732 97 AccI GT'mk_AC Cuts at: 0 2789 2829 Size: 2789 40 Acc65I G'GTAC_C Cuts at: 0 340 2829 Size: 340 2489 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 611 1172 2077 2493 2584 2829 Size: 611 561 905 416 91 245 Fragments arranged by size: 905 611 561 416 245 91 AfeI AGC'GCT Cuts at: 0 2083 2164 2829 Size: 2083 81 665 Fragments arranged by size: 2083 665 81 AflIII A'CryG_T Cuts at: 0 1552 2294 2829 Size: 1552 742 535 Fragments arranged by size: 1552 742 535 AleI CACnn'nnGTG Cuts at: 0 904 1564 2829 Size: 904 660 1265 Fragments arranged by size: 1265 904 660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1582 2829 Size: 1582 1247 AlwNI CAG_nnn'CTG Cuts at: 0 1038 2718 2829 Size: 1038 1680 111 Fragments arranged by size: 1680 1038 111 ApaI G_GGCC'C Cuts at: 0 83 659 1079 1242 1281 2829 Size: 83 576 420 163 39 1548 Fragments arranged by size: 1548 576 420 163 83 39 ApaLI G'TGCA_C Cuts at: 0 1954 2829 Size: 1954 875 ApoI r'AATT_y Cuts at: 0 1397 2829 Size: 1397 1432 AvaI C'yCGr_G Cuts at: 0 75 108 148 196 214 837 1757 2323 Size: 75 33 40 48 18 623 920 566 Cuts at: 2323 2769 2829 Size: 446 60 Fragments arranged by size: 920 623 566 446 75 60 48 40 33 18 BamHI G'GATC_C Cuts at: 0 2000 2051 2829 Size: 2000 51 778 Fragments arranged by size: 2000 778 51 BanI G'GyrC_C Cuts at: 0 157 340 571 1522 1714 2452 2829 Size: 157 183 231 951 192 738 377 Fragments arranged by size: 951 738 377 231 192 183 157 BanII G_rGCy'C Cuts at: 0 83 130 206 214 466 507 659 829 Size: 83 47 76 8 252 41 152 170 Cuts at: 829 981 1079 1141 1242 1281 2258 2777 2829 Size: 152 98 62 101 39 977 519 52 Fragments arranged by size: 977 519 252 170 152 152 101 98 83 76 62 52 47 41 39 8 BbeI G_GCGC'C Cuts at: 0 161 575 2829 Size: 161 414 2254 Fragments arranged by size: 2254 414 161 BbsI GAAGACnn'nnnn_ Cuts at: 0 1835 2453 2482 2829 Size: 1835 618 29 347 Fragments arranged by size: 1835 618 347 29 BbvCI CC'TCA_GC Cuts at: 0 1362 2113 2829 Size: 1362 751 716 Fragments arranged by size: 1362 751 716 BciVI GTATCCnnnnn_n' Cuts at: 0 781 2829 Size: 781 2048 BclI T'GATC_A Cuts at: 0 35 2829 Size: 35 2794 BglI GCCn_nnn'nGGC Cuts at: 0 218 1543 1760 2829 Size: 218 1325 217 1069 Fragments arranged by size: 1325 1069 218 217 BglII A'GATC_T Cuts at: 0 1449 2829 Size: 1449 1380 BlpI GC'TnA_GC Cuts at: 0 124 2829 Size: 124 2705 Bme1580I G_kGCm'C Cuts at: 0 83 526 659 1079 1242 1281 1471 1525 Size: 83 443 133 420 163 39 190 54 Cuts at: 1525 1717 1958 2829 Size: 192 241 871 Fragments arranged by size: 871 443 420 241 192 190 163 133 83 54 39 BmrI ACTGGGnnnn_n' Cuts at: 0 77 938 2829 Size: 77 861 1891 Fragments arranged by size: 1891 861 77 BmtI G_CTAG'C Cuts at: 0 887 2829 Size: 887 1942 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1863 2829 Size: 1863 966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1438 1491 1833 2266 2829 Size: 1438 53 342 433 563 Fragments arranged by size: 1438 563 433 342 53 Bpu10I CC'TnA_GC Cuts at: 0 734 1216 1362 1859 2113 2829 Size: 734 482 146 497 254 716 Fragments arranged by size: 734 716 497 482 254 146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1009 2829 Size: 1009 1820 BsaI GGTCTCn'nnnn_ Cuts at: 0 1967 2386 2829 Size: 1967 419 443 Fragments arranged by size: 1967 443 419 BsaBI GATnn'nnATC Cuts at: 0 2230 2829 Size: 2230 599 BsaHI Gr'CG_yC Cuts at: 0 97 158 572 2729 2829 Size: 97 61 414 2157 100 Fragments arranged by size: 2157 414 100 97 61 BsaWI w'CCGG_w Cuts at: 0 1623 2829 Size: 1623 1206 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 966 996 1312 1342 2829 Size: 966 30 316 30 1487 Fragments arranged by size: 1487 966 316 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 126 214 379 660 1186 1276 1753 1791 Size: 126 88 165 281 526 90 477 38 Cuts at: 1791 1806 1852 2263 2266 2353 2623 2829 Size: 15 46 411 3 87 270 206 Fragments arranged by size: 526 477 411 281 270 206 165 126 90 88 87 46 38 15 3 BseYI C'CCAG_C Cuts at: 0 177 658 1056 1338 1678 2278 2829 Size: 177 481 398 282 340 600 551 Fragments arranged by size: 600 551 481 398 340 282 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 1513 2829 Size: 433 1080 1316 Fragments arranged by size: 1316 1080 433 BsiEI CG_ry'CG Cuts at: 0 234 708 2829 Size: 234 474 2121 Fragments arranged by size: 2121 474 234 BsiHKAI G_wGCw'C Cuts at: 0 466 1958 2258 2829 Size: 466 1492 300 571 Fragments arranged by size: 1492 571 466 300 BsmI GAATG_Cn' Cuts at: 0 695 1418 2829 Size: 695 723 1411 Fragments arranged by size: 1411 723 695 BsmBI CGTCTCn'nnnn_ Cuts at: 0 88 1168 1735 2829 Size: 88 1080 567 1094 Fragments arranged by size: 1094 1080 567 88 Bsp1286I G_dGCh'C Cuts at: 0 83 130 206 214 466 507 526 659 Size: 83 47 76 8 252 41 19 133 Cuts at: 659 829 981 1079 1141 1242 1281 1471 1525 Size: 170 152 98 62 101 39 190 54 Cuts at: 1525 1717 1958 2258 2777 2829 Size: 192 241 300 519 52 Fragments arranged by size: 519 300 252 241 192 190 170 152 133 101 98 83 76 62 54 52 47 41 39 19 8 BspEI T'CCGG_A Cuts at: 0 1623 2829 Size: 1623 1206 BspHI T'CATG_A Cuts at: 0 38 2829 Size: 38 2791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 315 405 666 1004 1698 1775 2136 2192 Size: 315 90 261 338 694 77 361 56 Cuts at: 2192 2484 2829 Size: 292 345 Fragments arranged by size: 694 361 345 338 315 292 261 90 77 56 BsrBI CCG'CTC Cuts at: 0 65 230 2829 Size: 65 165 2599 Fragments arranged by size: 2599 165 65 BsrDI GCAATG_nn' Cuts at: 0 619 2829 Size: 619 2210 BsrFI r'CCGG_y Cuts at: 0 233 317 337 1537 2093 2558 2829 Size: 233 84 20 1200 556 465 271 Fragments arranged by size: 1200 556 465 271 233 84 20 BssSI C'ACGA_G Cuts at: 0 2611 2829 Size: 2611 218 BstAPI GCAn_nnn'nTGC Cuts at: 0 411 2829 Size: 411 2418 BstBI TT'CG_AA Cuts at: 0 2574 2829 Size: 2574 255 BstEII G'GTnAC_C Cuts at: 0 2496 2829 Size: 2496 333 BstXI CCAn_nnnn'nTGG Cuts at: 0 807 906 1242 1726 2829 Size: 807 99 336 484 1103 Fragments arranged by size: 1103 807 484 336 99 BstYI r'GATC_y Cuts at: 0 386 948 1449 2000 2051 2829 Size: 386 562 501 551 51 778 Fragments arranged by size: 778 562 551 501 386 51 Bsu36I CC'TnA_GG Cuts at: 0 1259 1660 1774 2705 2829 Size: 1259 401 114 931 124 Fragments arranged by size: 1259 931 401 124 114 BtgI C'CryG_G Cuts at: 0 899 1283 1526 1805 2136 2371 2405 2829 Size: 899 384 243 279 331 235 34 424 Fragments arranged by size: 899 424 384 331 279 243 235 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1097 2829 Size: 1097 1732 BtsI GCAGTG_nn' Cuts at: 0 125 267 1050 2389 2829 Size: 125 142 783 1339 440 Fragments arranged by size: 1339 783 440 142 125 DrdI GACnn_nn'nnGTC Cuts at: 0 2805 2829 Size: 2805 24 EaeI y'GGCC_r Cuts at: 0 231 705 896 2189 2408 2829 Size: 231 474 191 1293 219 421 Fragments arranged by size: 1293 474 421 231 219 191 EagI C'GGCC_G Cuts at: 0 231 705 2829 Size: 231 474 2124 Fragments arranged by size: 2124 474 231 EarI CTCTTCn'nnn_ Cuts at: 0 55 175 704 1794 2641 2829 Size: 55 120 529 1090 847 188 Fragments arranged by size: 1090 847 529 188 120 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 240 456 2829 Size: 240 216 2373 Fragments arranged by size: 2373 240 216 EcoICRI GAG'CTC Cuts at: 0 464 2256 2829 Size: 464 1792 573 Fragments arranged by size: 1792 573 464 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 611 1172 1438 1491 1833 2077 2266 2493 Size: 611 561 266 53 342 244 189 227 Cuts at: 2493 2584 2829 Size: 91 245 Fragments arranged by size: 611 561 342 266 245 244 227 189 91 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 565 1663 2248 2287 2704 2829 Size: 565 1098 585 39 417 125 Fragments arranged by size: 1098 585 565 417 125 39 EcoO109I rG'GnC_Cy Cuts at: 0 79 558 655 993 1011 1076 1238 1239 Size: 79 479 97 338 18 65 162 1 Cuts at: 1239 1255 1277 1278 1328 1730 2117 2237 2328 Size: 16 22 1 50 402 387 120 91 Cuts at: 2328 2829 Size: 501 Fragments arranged by size: 501 479 402 387 338 162 120 97 91 79 65 50 22 18 16 1 1 EcoRI G'AATT_C Cuts at: 0 1397 2829 Size: 1397 1432 EcoRV GAT'ATC Cuts at: 0 1117 2829 Size: 1117 1712 HaeII r_GCGC'y Cuts at: 0 50 161 481 575 2085 2166 2829 Size: 50 111 320 94 1510 81 663 Fragments arranged by size: 1510 663 320 111 94 81 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 175 207 1312 1344 1864 1896 2185 2217 Size: 175 32 1105 32 520 32 289 32 Cuts at: 2217 2535 2567 2829 Size: 318 32 262 Fragments arranged by size: 1105 520 318 289 262 175 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2790 2829 Size: 2790 39 KasI G'GCGC_C Cuts at: 0 157 571 2829 Size: 157 414 2258 Fragments arranged by size: 2258 414 157 KpnI G_GTAC'C Cuts at: 0 344 2829 Size: 344 2485 MluI A'CGCG_T Cuts at: 0 1552 2829 Size: 1552 1277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1334 2829 Size: 1334 1495 MscI TGG'CCA Cuts at: 0 898 2829 Size: 898 1931 MslI CAynn'nnrTG Cuts at: 0 805 904 1564 2141 2829 Size: 805 99 660 577 688 Fragments arranged by size: 805 688 660 577 99 MspA1I CmG'CkG Cuts at: 0 257 269 788 1596 2007 2038 2355 2829 Size: 257 12 519 808 411 31 317 474 Fragments arranged by size: 808 519 474 411 317 257 31 12 NaeI GCC'GGC Cuts at: 0 235 2829 Size: 235 2594 NarI GG'CG_CC Cuts at: 0 158 572 2829 Size: 158 414 2257 Fragments arranged by size: 2257 414 158 NcoI C'CATG_G Cuts at: 0 1526 2136 2829 Size: 1526 610 693 Fragments arranged by size: 1526 693 610 NgoMIV G'CCGG_C Cuts at: 0 233 2829 Size: 233 2596 NheI G'CTAG_C Cuts at: 0 883 2829 Size: 883 1946 NspI r_CATG'y Cuts at: 0 438 1134 2298 2382 2829 Size: 438 696 1164 84 447 Fragments arranged by size: 1164 696 447 438 84 PasI CC'CwG_GG Cuts at: 0 1096 1235 2415 2829 Size: 1096 139 1180 414 Fragments arranged by size: 1180 1096 414 139 PciI A'CATG_T Cuts at: 0 2294 2829 Size: 2294 535 PflMI CCAn_nnn'nTGG Cuts at: 0 429 1514 2669 2829 Size: 429 1085 1155 160 Fragments arranged by size: 1155 1085 429 160 PfoI T'CCnGG_A Cuts at: 0 640 2829 Size: 640 2189 PpuMI rG'GwC_Cy Cuts at: 0 558 993 1328 2237 2829 Size: 558 435 335 909 592 Fragments arranged by size: 909 592 558 435 335 PspOMI G'GGCC_C Cuts at: 0 79 655 1075 1238 1277 2829 Size: 79 576 420 163 39 1552 Fragments arranged by size: 1552 576 420 163 79 39 PstI C_TGCA'G Cuts at: 0 1001 1675 2723 2829 Size: 1001 674 1048 106 Fragments arranged by size: 1048 1001 674 106 PvuII CAG'CTG Cuts at: 0 2007 2038 2829 Size: 2007 31 791 Fragments arranged by size: 2007 791 31 SacI G_AGCT'C Cuts at: 0 466 2258 2829 Size: 466 1792 571 Fragments arranged by size: 1792 571 466 SalI G'TCGA_C Cuts at: 0 2788 2829 Size: 2788 41 SanDI GG'GwC_CC Cuts at: 0 1328 2829 Size: 1328 1501 SapI GCTCTTCn'nnn_ Cuts at: 0 55 2829 Size: 55 2774 SfcI C'TryA_G Cuts at: 0 719 997 1671 2719 2829 Size: 719 278 674 1048 110 Fragments arranged by size: 1048 719 674 278 110 SfoI GGC'GCC Cuts at: 0 159 573 2829 Size: 159 414 2256 Fragments arranged by size: 2256 414 159 SmaI CCC'GGG Cuts at: 0 77 150 216 839 2829 Size: 77 73 66 623 1990 Fragments arranged by size: 1990 623 77 73 66 SmlI C'TyrA_G Cuts at: 0 988 2829 Size: 988 1841 SphI G_CATG'C Cuts at: 0 1134 2829 Size: 1134 1695 StuI AGG'CCT Cuts at: 0 1669 2506 2829 Size: 1669 837 323 Fragments arranged by size: 1669 837 323 StyI C'CwwG_G Cuts at: 0 1526 1733 2136 2829 Size: 1526 207 403 693 Fragments arranged by size: 1526 693 403 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 813 1477 1732 2815 2829 Size: 813 664 255 1083 14 Fragments arranged by size: 1083 813 664 255 14 TatI w'GTAC_w Cuts at: 0 8 2829 Size: 8 2821 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 966 998 2829 Size: 966 32 1831 Fragments arranged by size: 1831 966 32 Tth111I GACn'n_nGTC Cuts at: 0 597 2829 Size: 597 2232 XbaI T'CTAG_A Cuts at: 0 2583 2829 Size: 2583 246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2412 2617 2829 Size: 2412 205 212 Fragments arranged by size: 2412 212 205 XmaI C'CCGG_G Cuts at: 0 75 148 214 837 2829 Size: 75 73 66 623 1992 Fragments arranged by size: 1992 623 75 73 66 XmnI GAAnn'nnTTC Cuts at: 0 1401 2829 Size: 1401 1428 ZraI GAC'GTC Cuts at: 0 2730 2829 Size: 2730 99 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SalI SanDI SapI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BcgI BfrBI BmgBI BsaAI BsiWI BsrGI BssHII BstZ17I ClaI CspCI DraI DraIII FalI FseI FspI FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XhoI