Restriction map for
pFN21AB5803
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BclI BcnI
BfaI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaI HpaII KasI
KpnI KspI MaeI MamI MluI
MroI MslI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 115 CCGC CCGC(-3/-1)
AflII 1113 CTTAAG C^TTAAG
AlwI 1173 GGATC GGATC(4/5)
AlwNI 457 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 855 GGACC G^G[AT]CC
BalI 578 TGGCCA TGG^CCA
BanI 142 GGCACC G^G[CT][GA]CC
BcgI 62 GCAGCACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 104 GTATCC GTATCC(6/5)
BfrI 1113 CTTAAG C^TTAAG
BglII 556 AGATCT A^GATCT
BsmAI 459 GAGAC GTCTC(1/5)
BsmI 35 GAATGC GAATGC(1/-1)
BstF5I 966 GGATG GGATG(2/0)
BstYI 556 AGATCT [GA]^GATC[CT]
Cac8I 927 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 855 GGACC G^G[ACGT]CC
EaeI 578 TGGCCA [CT]^GGCC[GA]
EarI 442 GAAGAG CTCTTC(1/4)
EcoT14I 546 CCTTGG C^C[AT][AT]GG
FokI 966 GGATG GGATG(9/13)
HaeIII 579 GGCC GG^CC
HindIII 658 AAGCTT A^AGCTT
HphI 1062 TCACC GGTGA(8/7)
Ksp632I 442 GAAGAG CTCTTC(1/4)
MfeI 938 CAATTG C^AATTG
MflI 556 AGATCT [GA]^GATC[CT]
MluNI 578 TGGCCA TGG^CCA
MscI 578 TGGCCA TGG^CCA
MunI 938 CAATTG C^AATTG
MwoI 117 GCTTTCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 588 ACATGC [GA]CATG^[CT]
SapI 442 GAAGAGC GCTCTTC(1/4)
Sau96I 855 GGACC G^G[ACGT]CC
SmlI 1113 CTTAAG C^T[CT][GA]AG
StyI 546 CCTTGG C^C[AT][AT]GG
XhoII 556 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AseI 361 ATTAAT AT^TAAT
751 ATTAAT
AsnI 361 ATTAAT AT^TAAT
751 ATTAAT
BbsI 43 GAAGAC GAAGAC(2/6)
690 GAAGAC
BpmI 109 CTGGAG CTGGAG(16/14)
1180 CTGGAG
BpuAI 43 GAAGAC GAAGAC(2/6)
690 GAAGAC
BsiYI 33 CCGAATGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
957 CCACATGGAGG
BslI 33 CCGAATGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
957 CCACATGGAGG
BsmFI 89 GGGAC GGGAC(10/14)
701 GGGAC
Bsp1407I 643 TGTACA T^GTACA
1222 TGTACA
BsrDI 533 GCAATG GCAATG(2/0)
629 GCAATG
BsrGI 643 TGTACA T^GTACA
1222 TGTACA
EcoRI 431 GAATTC G^AATTC
541 GAATTC
MaeII 413 ACGT A^CGT
1168 ACGT
MaeIII 410 GTGAC ^GT[ACGT]AC
1244 GTGAC
NlaIV 111 GGAGCC GG[ACGT]^[ACGT]CC
142 GGCACC
PshBI 361 ATTAAT AT^TAAT
751 ATTAAT
PstI 60 CTGCAG CTGCA^G
902 CTGCAG
SfaNI 967 GATGC GCATC(5/9)
741 GCATC
SfcI 60 CTGCAG C^T[GA][CT]AG
902 CTGCAG
SspBI 643 TGTACA T^GTACA
1222 TGTACA
Tsp45I 410 GTGAC ^GT[GC]AC
1244 GTGAC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbvI BsaJI BsrI BssKI BstNI
DdeI DpnII EcoRII Fnu4HI ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
Tru9I TseI Tsp509I TspRI XmnI