Restriction map for
pFN21AB5803
(Circular) (Six-base) MAPSORT of: pFN21AB5803.seq Check: 8163 from: 1 to: 6055
pFN21AB5803 6055 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2438 2686 4118 5502 5770 1632
Size: 620 186 248 1432 1384 268 1917
Fragments arranged by size:
1917 1432 1384 620 268 248 186
AflII C'TTAA_G
Cuts at: 820 1017 3118 820
Size: 197 2101 3757
Fragments arranged by size:
3757 2101 197
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 2467 3798 5370 2467
Size: 1331 1572 3152
Fragments arranged by size:
3152 1572 1331
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2436 2475 2546 2639 2981 3019 3364 3590
Size: 943 39 71 93 342 38 345 226
Cuts at: 3590 3742 5851 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 943 345 342 226 152 93
71 39 38
AseI AT'TA_AT
Cuts at: 160 1032 2367 2757 4609 160
Size: 872 1335 390 1852 1606
Fragments arranged by size:
1852 1606 1335 872 390
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 3376 4831 618
Size: 325 217 627 360 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 360 325 217
BanII G_rGCy'C
Cuts at: 1391 3374 1391
Size: 1983 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 2702 928
Size: 971 156 647 4281
Fragments arranged by size:
4281 971 647 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2056 2090 4246 4280 1903
Size: 34 119 34 2156 34 3678
Fragments arranged by size:
3678 2156 119 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2120 3947 5157 2120
Size: 1827 1210 3018
Fragments arranged by size:
3018 1827 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2561 6050 2561
Size: 3489 2566
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 4116 5272 1790
Size: 2326 1156 2573
Fragments arranged by size:
2573 2326 1156
BmgBI CAC'GTC
Cuts at: 1318 3174 1318
Size: 1856 4199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 4744 442
Size: 416 53 83 399 24 2369 958 1753
Fragments arranged by size:
2369 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 3206 4715 1239
Size: 896 1071 1509 2579
Fragments arranged by size:
2579 1509 1071 896
Bpu10I CC'TnA_GC
Cuts at: 3261 3261
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4718 882
Size: 617 3219 2219
Fragments arranged by size:
3219 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3862 4244 4879 275
Size: 53 83 186 936 2329 382 635 1451
Fragments arranged by size:
2329 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6055
BseYI C'CCAG_C
Cuts at: 5258 5754 5258
Size: 496 5559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4116 4201 5272 4116
Size: 85 1071 4899
Fragments arranged by size:
4899 1071 85
BsmI GAATG_Cn'
Cuts at: 2046 3566 3659 2046
Size: 1520 93 4442
Fragments arranged by size:
4442 1520 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3374 4116 4201 5272 1391
Size: 399 1584 742 85 1071 2174
Fragments arranged by size:
2174 1584 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3410 844
Size: 2566 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2545 2641 4550 4724 65
Size: 1183 1297 96 1909 174 1396
Fragments arranged by size:
1909 1396 1297 1183 174 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2648 3227 96
Size: 2552 579 2924
Fragments arranged by size:
2924 2552 579
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2561 3385 3752 4137 4154 5606 5692 5704
Size: 589 824 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 824 589 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2373 2684 3571 4365 4385 2373
Size: 311 887 794 20 4043
Fragments arranged by size:
4043 887 794 311 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2583 3502 4391
Size: 54 1425 50 275 18 753 919 889
Cuts at: 4391 6020 8
Size: 1629 43
Fragments arranged by size:
1629 1425 919 889 753 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 3985 1353
Size: 1089 1543 3423
Fragments arranged by size:
3423 1543 1089
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2252 2438 2686 3206 4118 4715
Size: 393 503 117 186 248 520 912 597
Cuts at: 4715 5502 5770 1239
Size: 787 268 1524
Fragments arranged by size:
1524 912 787 597 520 503 393 268
248 186 117
EcoRI G'AATT_C
Cuts at: 2436 2546 3364 3742 2436
Size: 110 818 378 4749
Fragments arranged by size:
4749 818 378 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2243 2567 2243
Size: 324 5731
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2775 2807 2864 2896 3163
Size: 32 135 32 1203 32 57 32 267
Cuts at: 3163 3195 4696 4728 4770 4802 1373
Size: 32 1501 32 42 32 2626
Fragments arranged by size:
2626 1501 1203 267 135 57 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2663 748
Size: 1915 4140
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 2943 3654 2943
Size: 711 5344
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2817 3224 5169 5353 1305
Size: 1512 407 1945 184 2007
Fragments arranged by size:
2007 1945 1512 407 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2585 10
Size: 54 1425 343 753 3480
Fragments arranged by size:
3480 1425 753 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4013 4372 4531 518
Size: 648 2847 359 159 2042
Fragments arranged by size:
2847 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3471 4148 5296 5541 1606
Size: 1865 677 1148 245 2120
Fragments arranged by size:
2120 1865 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4564
Fragments arranged by size:
4564 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2597 3413 4958 1132
Size: 97 1368 816 1545 2229
Fragments arranged by size:
2229 1545 1368 816 97
PasI CC'CwG_GG
Cuts at: 1348 2023 1348
Size: 675 5380
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PfoI T'CCnGG_A
Cuts at: 2112 2112
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2069 2911 3407 830
Size: 1239 842 496 3478
Fragments arranged by size:
3478 1239 842 496
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SapI GCTCTTCn'nnn_
Cuts at: 2442 2442
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2065 2907 3403 4538 5219 5410 826
Size: 219 1020 842 496 1135 681 191 1471
Fragments arranged by size:
1471 1135 1020 842 681 496 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3118 4159 5060 5322 820
Size: 197 926 1175 1041 901 262 1553
Fragments arranged by size:
1553 1175 1041 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 3352 152
Size: 3200 2855
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2551 3494 513
Size: 552 939 547 943 3074
Fragments arranged by size:
3074 943 939 552 547
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2648 2685 3227
Size: 274 80 33 51 1443 671 37 542
Cuts at: 3227 4301 6013 96
Size: 1074 1712 138
Fragments arranged by size:
1712 1443 1074 671 542 274 138 80
51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 2202 2321 2368 2451 2699 2829 3279 4184 2202
Size: 119 47 83 248 130 450 905 4073
Fragments arranged by size:
4073 905 450 248 130 119 83 47
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI