################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AccI AccII AccIII AclI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp700I AvaI AviII AvrII BamHI BbeI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I FauI FseI FspI HaeII HgaI HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 1616 GACGTC GACGT^C Acc65I 144 GGTACC G^GTACC AcyI 1616 GACGTC G[GA]^CG[CT]C AflIII 757 ACATGT A^C[GA][CT]GT AhdI 1616 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1167 GTGCAC G^TGCAC AocI 1405 CCTCAGG CC^T[ACGT]AGG ApaLI 1167 GTGCAC G^TGCAC Asp718I 144 GGTACC G^GTACC AspEI 1616 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1012 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 144 GGTACC G^G[CT][GA]CC BanII 1245 GGGCTC G[GA]GC[CT]^C BbrPI 951 CACGTG CAC^GTG BbsI 262 GAAGAC GAAGAC(2/6) BclI 179 TGATCA T^GATCA BcnI 1940 CCCGG CC^[GC]GG BpuAI 262 GAAGAC GAAGAC(2/6) BsaAI 951 CACGTG [CT]AC^GT[GA] BsaHI 1616 GACGTC G[GA]^CG[CT]C BsmI 351 GCATTC GAATGC(1/-1) BspDI 763 ATCGAT AT^CGAT BspHI 6 TCATGA T^CATGA BspLU11I 757 ACATGT A^CATGT BsrDI 13 GCAATG GCAATG(2/0) BsrFI 899 ACCGGC [GA]^CCGG[CT] BssSI 1713 CACGAG CACGAG(-5/-1) BstAPI 2167 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1405 CCTCAGG CC^T[ACGT]AGG CfoI 1068 GCGC GCG^C Cfr10I 899 ACCGGC [GA]^CCGG[CT] ClaI 763 ATCGAT AT^CGAT DrdI 1012 GACATTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1616 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1405 CCTCAGG CC^T[ACGT]AGG EcoNI 1083 CCTACTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 403 GAATTC G^AATTC EcoRV 1389 GATATC GAT^ATC EcoT22I 1483 ATGCAT ATGCA^T FbaI 179 TGATCA T^GATCA HhaI 1068 GCGC GCG^C Hin1I 1616 GACGTC G[GA]^CG[CT]C HinP1I 1068 GCGC G^CGC KpnI 144 GGTACC GGTAC^C NciI 1940 CCCGG CC^[GC]GG NdeI 1808 CATATG CA^TATG NsiI 1483 ATGCAT ATGCA^T PflFI 1012 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1139 GACTC GAGTC(4/5) PmaCI 951 CACGTG CAC^GTG PmlI 951 CACGTG CAC^GTG PvuII 1796 CAGCTG CAG^CTG RcaI 6 TCATGA T^CATGA ScaI 1447 AGTACT AGT^ACT SpeI 1054 ACTAGT A^CTAGT SphI 395 GCATGC GCATG^C TaqI 764 TCGA T^CGA Tth111I 1012 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 764 TCGA T^CGA XcmI 1751 CCAGATGCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 220 CCGC CCGC(-3/-1) 374 GCGG AlwNI 62 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 164 CAGCTCCTG AspHI 524 GAGCAC G[AT]GC[AT]^C 1167 GTGCAC BalI 77 TGGCCA TGG^CCA 1676 TGGCCA BbvCI 1793 CCTCAGC CCTCAGC(-5/-2) 172 GCTGAGG BsaBI 319 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1969 GATAAAAATC BseRI 103 CTCCTC GAGGAG(10/8) 1403 CTCCTC BsgI 34 GTGCAG GTGCAG(16/14) 1726 GTGCAG BsiHKAI 524 GAGCAC G[AT]GC[AT]^C 1167 GTGCAC Bsp1407I 859 TGTACA T^GTACA 2058 TGTACA BsrGI 859 TGTACA T^GTACA 2058 TGTACA BstYI 313 GGATCT [GA]^GATC[CT] 1496 AGATCC DsaI 1500 CCGTGG C^C[GA][CT]GG 1592 CCGTGG EaeI 77 TGGCCA [CT]^GGCC[GA] 1676 TGGCCA HincII 232 GTTGAC GT[CT]^[GA]AC 682 GTTGAC HindII 232 GTTGAC GT[CT]^[GA]AC 682 GTTGAC MamI 319 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1969 GATAAAAATC MflI 313 GGATCT [GA]^GATC[CT] 1496 AGATCC MluNI 77 TGGCCA TGG^CCA 1676 TGGCCA MscI 77 TGGCCA TGG^CCA 1676 TGGCCA MslI 1476 CATTCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2139 CATCCTGGTG MspA1I 372 CAGCGG C[AC]G^C[GT]G 1796 CAGCTG PflMI 1423 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1807 CCATATGGTGG SapI 718 GAAGAGC GCTCTTC(1/4) 1461 GAAGAGC SspBI 859 TGTACA T^GTACA 2058 TGTACA StuI 1409 AGGCCT AGG^CCT 1963 AGGCCT Van91I 1423 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1807 CCATATGGTGG XhoII 313 GGATCT [GA]^GATC[CT] 1496 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI