(Circular) (Six-base) MAPSORT of: pFN21AE3296.seq Check: 7132 from: 1 to: 7027 pFN21AE3296 7027 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2726 2620 Size: 106 6921 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 4837 278 Size: 53 83 186 936 2089 1212 2468 Fragments arranged by size: 2468 2089 1212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4370 1937 Size: 2433 4594 Acc65I G'GTAC_C Cuts at: 2149 4348 2149 Size: 2199 4828 AclI AA'CG_TT Cuts at: 1563 5154 5527 1563 Size: 3591 373 3063 Fragments arranged by size: 3591 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2640 3113 5090 6474 6742 1632 Size: 651 357 473 1977 1384 268 1917 Fragments arranged by size: 1977 1917 1384 651 473 357 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6830 AflIII A'CryG_T Cuts at: 1203 2762 5926 6903 1203 Size: 1559 3164 977 1327 Fragments arranged by size: 3164 1559 1327 977 AgeI A'CCGG_T Cuts at: 5858 5858 Size: 7027 AhdI GACnn_n'nnGTC Cuts at: 3626 5756 3626 Size: 2130 4897 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7027 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3367 3746 1184 Size: 2183 379 4465 Fragments arranged by size: 4465 2183 379 AlwNI CAG_nnn'CTG Cuts at: 2072 2174 4770 6342 2072 Size: 102 2596 1572 2757 Fragments arranged by size: 2757 2596 1572 102 ApaLI G'TGCA_C Cuts at: 3172 5084 6240 3172 Size: 1912 1156 3959 Fragments arranged by size: 3959 1912 1156 ApoI r'AATT_y Cuts at: 1493 2408 3232 4174 4336 4562 4714 6823 1493 Size: 915 824 942 162 226 152 2109 1697 Fragments arranged by size: 2109 1697 942 915 824 226 162 152 AseI AT'TA_AT Cuts at: 160 1032 5581 160 Size: 872 4549 1606 Fragments arranged by size: 4549 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 AvaI C'yCGr_G Cuts at: 1870 1943 4352 1870 Size: 73 2409 4545 Fragments arranged by size: 4545 2409 73 BamHI G'GATC_C Cuts at: 4357 4724 4357 Size: 367 6660 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2149 4348 5803 618 Size: 325 217 627 362 2199 1455 1842 Fragments arranged by size: 2199 1842 1455 627 362 325 217 BanII G_rGCy'C Cuts at: 1391 3254 4346 1391 Size: 1863 1092 4072 Fragments arranged by size: 4072 1863 1092 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2274 928 Size: 971 375 5681 Fragments arranged by size: 5681 971 375 BbvCI CC'TCA_GC Cuts at: 2178 3799 2178 Size: 1621 5406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5218 5252 1903 Size: 34 3281 34 3678 Fragments arranged by size: 3678 3281 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4919 6129 4919 Size: 1210 5817 BclI T'GATC_A Cuts at: 1548 2184 1548 Size: 636 6391 BfrBI ATG'CAT Cuts at: 3490 3490 Size: 7027 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5638 136 Size: 107 122 71 631 605 3966 1525 Fragments arranged by size: 3966 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7022 7022 Size: 7027 BlpI GC'TnA_GC Cuts at: 4444 4444 Size: 7027 Bme1580I G_kGCm'C Cuts at: 1790 3176 5088 6244 1790 Size: 1386 1912 1156 2573 Fragments arranged by size: 2573 1912 1386 1156 BmgBI CAC'GTC Cuts at: 1318 3709 1318 Size: 2391 4636 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2983 4167 4758 Size: 416 53 83 399 24 1566 1184 591 Cuts at: 4758 5716 442 Size: 958 1753 Fragments arranged by size: 1753 1566 1184 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7027 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3958 3958 Size: 7027 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2147 2198 2727 3268 3605 3652 4199 Size: 857 51 51 529 541 337 47 547 Cuts at: 4199 5687 1239 Size: 1488 2579 Fragments arranged by size: 2579 1488 857 547 541 529 337 51 51 47 Bpu10I CC'TnA_GC Cuts at: 2178 3107 3269 3799 2178 Size: 929 162 530 5406 Fragments arranged by size: 5406 929 530 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5152 6017 6315 5152 Size: 865 298 5864 Fragments arranged by size: 5864 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2410 2505 3775 5690 882 Size: 617 911 95 1270 1915 2219 Fragments arranged by size: 2219 1915 1270 911 617 95 BsaAI yAC'GTr Cuts at: 493 1204 2958 4818 493 Size: 711 1754 1860 2702 Fragments arranged by size: 2702 1860 1754 711 BsaBI GATnn'nnATC Cuts at: 2328 3978 7021 2328 Size: 1650 3043 2334 Fragments arranged by size: 3043 2334 1650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3622 4834 5216 5851 Size: 53 83 186 936 2089 1212 382 635 Cuts at: 5851 275 Size: 1451 Fragments arranged by size: 2089 1451 1212 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5460 5858 6132 6279 6690 5460 Size: 398 274 147 411 5797 Fragments arranged by size: 5797 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 2196 2226 2670 Size: 30 165 30 688 30 75 30 444 Cuts at: 2670 2700 1178 Size: 30 5505 Fragments arranged by size: 5505 688 444 165 75 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 3399 1186 Size: 913 1300 4814 Fragments arranged by size: 4814 1300 913 BseYI C'CCAG_C Cuts at: 2341 2565 2894 6230 6726 2341 Size: 224 329 3336 496 2642 Fragments arranged by size: 3336 2642 496 329 224 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 3752 1163 Size: 573 324 1692 4438 Fragments arranged by size: 4438 1692 573 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4477 5238 6266 1540 Size: 275 186 2476 761 1028 2301 Fragments arranged by size: 2476 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2533 3176 5088 5173 6244 2533 Size: 643 1912 85 1071 3316 Fragments arranged by size: 3316 1912 1071 643 85 BsmI GAATG_Cn' Cuts at: 2356 4538 4631 2356 Size: 2182 93 4752 Fragments arranged by size: 4752 2182 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2533 3176 3254 4346 5088 5173 6244 Size: 399 743 643 78 1092 742 85 1071 Cuts at: 6244 1391 Size: 2174 Fragments arranged by size: 2174 1092 1071 743 742 643 399 85 78 BspEI T'CCGG_A Cuts at: 6690 6690 Size: 7027 BspHI T'CATG_A Cuts at: 2011 4916 6646 2011 Size: 2905 1730 2392 Fragments arranged by size: 2905 2392 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 2726 3145 4382 844 Size: 1776 106 419 1237 3489 Fragments arranged by size: 3489 1776 1237 419 106 BsrBI CCG'CTC Cuts at: 1394 1823 4914 1394 Size: 429 3091 3507 Fragments arranged by size: 3507 3091 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2025 5522 5696 65 Size: 1183 777 3497 174 1396 Fragments arranged by size: 3497 1396 1183 777 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2904 5671 5858 1700 Size: 110 1094 2767 187 2869 Fragments arranged by size: 2869 2767 1094 187 110 BsrGI T'GTAC_A Cuts at: 96 2864 4063 96 Size: 2768 1199 3060 Fragments arranged by size: 3060 2768 1199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7027 BssSI C'ACGA_G Cuts at: 3718 5087 6099 3718 Size: 1369 1012 4646 Fragments arranged by size: 4646 1369 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4178 794 Size: 3384 3643 BstBI TT'CG_AA Cuts at: 5831 5831 Size: 7027 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7027 BstYI r'GATC_y Cuts at: 1972 2318 3501 4357 4724 5109 5126 6578 6664 Size: 346 1183 856 367 385 17 1452 86 Cuts at: 6664 6676 7022 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1183 856 385 367 346 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3411 3411 Size: 7027 BtgI C'CryG_G Cuts at: 513 1065 2004 3505 3597 513 Size: 552 939 1501 92 3943 Fragments arranged by size: 3943 1501 939 552 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6288 Fragments arranged by size: 6288 683 56 BtsI GCAGTG_nn' Cuts at: 4543 5337 5357 4543 Size: 794 20 6213 Fragments arranged by size: 6213 794 20 ClaI AT'CG_AT Cuts at: 1554 2769 1554 Size: 1215 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3579 3614 529 Size: 35 3015 35 3942 Fragments arranged by size: 3942 3015 35 35 DraI TTT'AAA Cuts at: 4331 4331 Size: 7027 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3023 6034 809 Size: 507 1707 3011 1802 Fragments arranged by size: 3011 1802 1707 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 3681 4474 Size: 54 1425 50 275 18 252 1599 793 Cuts at: 4474 5363 6992 8 Size: 889 1629 43 Fragments arranged by size: 1629 1599 1425 889 793 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4474 1537 Size: 275 2662 4090 Fragments arranged by size: 4090 2662 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2616 2718 3461 4957 1353 Size: 1263 102 743 1496 3423 Fragments arranged by size: 3423 1496 1263 743 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5614 5988 6134 387 Size: 1064 138 4025 374 146 1280 Fragments arranged by size: 4025 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2147 2198 2283 2640 2727 3113 Size: 393 464 51 51 85 357 87 386 Cuts at: 3113 3268 3605 3652 4199 5090 5687 6474 6742 Size: 155 337 47 547 891 597 787 268 Cuts at: 6742 1239 Size: 1524 Fragments arranged by size: 1524 891 787 597 547 464 393 386 357 337 268 155 87 85 51 51 47 EcoNI CCTnn'n_nnAGG Cuts at: 3092 3092 Size: 7027 EcoO109I rG'GnC_Cy Cuts at: 2562 3157 3265 4082 4219 2562 Size: 595 108 817 137 5370 Fragments arranged by size: 5370 817 595 137 108 EcoRI G'AATT_C Cuts at: 2408 4336 4714 2408 Size: 1928 378 4721 Fragments arranged by size: 4721 1928 378 EcoRV GAT'ATC Cuts at: 3396 3396 Size: 7027 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3331 3331 Size: 7027 FspI TGC'GCA Cuts at: 5533 5533 Size: 7027 HaeII r_GCGC'y Cuts at: 1678 6174 1678 Size: 4496 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 2593 2625 3113 Size: 32 135 32 519 32 470 32 488 Cuts at: 3113 3145 3612 3644 5668 5700 5742 5774 1373 Size: 32 467 32 2024 32 42 32 2626 Fragments arranged by size: 2626 2024 519 488 470 467 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 2689 4371 4617 669 Size: 988 281 301 450 1682 246 3079 Fragments arranged by size: 3079 1682 988 450 301 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7027 HpaI GTT'AAC Cuts at: 4617 4617 Size: 7027 KpnI G_GTAC'C Cuts at: 2153 4352 2153 Size: 2199 4828 MfeI C'AATT_G Cuts at: 4626 4626 Size: 7027 MluI A'CGCG_T Cuts at: 6903 6903 Size: 7027 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3172 4224 6141 6325 1305 Size: 1867 1052 1917 184 2007 Fragments arranged by size: 2007 1917 1867 1052 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 3683 10 Size: 54 1425 343 252 1599 3354 Fragments arranged by size: 3354 1599 1425 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 3485 4148 4985 5344 5503 518 Size: 648 2319 663 837 359 159 2042 Fragments arranged by size: 2319 2042 837 663 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2379 3803 4443 5120 6268 6513 1606 Size: 773 1424 640 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 773 677 640 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5536 Fragments arranged by size: 5536 939 552 NdeI CA'TA_TG Cuts at: 387 3814 387 Size: 3427 3600 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7027 NotI GC'GGCC_GC Cuts at: 4474 4474 Size: 7027 NsiI A_TGCA'T Cuts at: 3492 3492 Size: 7027 NspI r_CATG'y Cuts at: 1132 1229 2404 2766 3787 4385 5930 1132 Size: 97 1175 362 1021 598 1545 2229 Fragments arranged by size: 2229 1545 1175 1021 598 362 97 PasI CC'CwG_GG Cuts at: 1348 3246 1348 Size: 1898 5129 PciI A'CATG_T Cuts at: 2762 5926 2762 Size: 3164 3863 PflMI CCAn_nnn'nTGG Cuts at: 1108 3434 3818 1108 Size: 2326 384 4317 Fragments arranged by size: 4317 2326 384 PfoI T'CCnGG_A Cuts at: 4215 4215 Size: 7027 PmeI GTTT'AAAC Cuts at: 4331 4331 Size: 7027 PmlI CAC'GTG Cuts at: 2958 2958 Size: 7027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5096 5128 6609 6641 5096 Size: 32 1481 32 5482 Fragments arranged by size: 5482 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2562 3157 3265 4082 4219 2562 Size: 595 108 817 137 5370 Fragments arranged by size: 5370 817 595 137 108 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7027 PsiI TTA'TAA Cuts at: 3612 4597 3612 Size: 985 6042 PstI C_TGCA'G Cuts at: 830 2388 2671 3156 4379 830 Size: 1558 283 485 1223 3478 Fragments arranged by size: 3478 1558 1223 485 283 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7027 PvuII CAG'CTG Cuts at: 3803 3803 Size: 7027 SalI G'TCGA_C Cuts at: 1936 4369 1936 Size: 2433 4594 SapI GCTCTTCn'nnn_ Cuts at: 2718 3461 2718 Size: 743 6284 SbfI CC_TGCA'GG Cuts at: 4379 4379 Size: 7027 ScaI AGT'ACT Cuts at: 3454 5275 6987 3454 Size: 1821 1712 3494 Fragments arranged by size: 3494 1821 1712 SfcI C'TryA_G Cuts at: 826 1045 2384 2667 3152 3964 4223 4375 5510 Size: 219 1339 283 485 812 259 152 1135 Cuts at: 5510 6191 6382 826 Size: 681 191 1471 Fragments arranged by size: 1471 1339 1135 812 681 485 283 259 219 191 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7027 SmaI CCC'GGG Cuts at: 1872 4354 1872 Size: 2482 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5131 6032 6294 820 Size: 197 926 3188 901 262 1553 Fragments arranged by size: 3188 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4818 493 Size: 4325 2702 SpeI A'CTAG_T Cuts at: 152 3059 152 Size: 2907 4120 SphI G_CATG'C Cuts at: 1132 2404 4385 1132 Size: 1272 1981 3774 Fragments arranged by size: 3774 1981 1272 SspI AAT'ATT Cuts at: 5 52 4951 5 Size: 47 4899 2081 Fragments arranged by size: 4899 2081 47 StuI AGG'CCT Cuts at: 1517 3416 3970 1517 Size: 1899 554 4574 Fragments arranged by size: 4574 1899 554 StyI C'CwwG_G Cuts at: 513 1065 2004 2092 3837 3900 4466 513 Size: 552 939 88 1745 63 566 3074 Fragments arranged by size: 3074 1745 939 566 552 88 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4244 5054 5071 5224 5409 5854 325 Size: 1255 2664 810 17 153 185 445 1498 Fragments arranged by size: 2664 1498 1255 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2864 3452 3477 Size: 274 80 33 51 1443 887 588 25 Cuts at: 3477 4063 4154 5273 6985 96 Size: 586 91 1119 1712 138 Fragments arranged by size: 1712 1443 1119 887 588 586 274 138 91 80 51 33 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2500 2532 4024 4056 6987 7019 2196 Size: 32 272 32 1492 32 2931 32 2204 Fragments arranged by size: 2931 2204 1492 272 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3020 1316 Size: 1704 5323 XbaI T'CTAG_A Cuts at: 4363 4363 Size: 7027 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3763 3763 Size: 7027 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7027 XmaI C'CCGG_G Cuts at: 1870 4352 1870 Size: 2482 4545 XmnI GAAnn'nnTTC Cuts at: 5156 5156 Size: 7027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 4835 276 Size: 53 83 186 936 2089 1212 2468 Fragments arranged by size: 2468 2089 1212 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI