Restriction map for
pFN21AE3296
(Linear) (Six-base) MAPSORT of: KIEE3296.seq Check: 896 from: 1 to: 2322
KIEE3296.orf
With 182 enzymes: SgfI *
July 3, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 721 2322
Size: 615 106 1601
Fragments arranged by size:
1601 615 106
AatII G_ACGT'C
Cuts at: 0 1620 2322
Size: 1620 702
Acc65I G'GTAC_C
Cuts at: 0 144 2322
Size: 144 2178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 635 1108 2322
Size: 278 357 473 1214
Fragments arranged by size:
1214 473 357 278
AflIII A'CryG_T
Cuts at: 0 757 2322
Size: 757 1565
AhdI GACnn_n'nnGTC
Cuts at: 0 1621 2322
Size: 1621 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1362 1741 2322
Size: 1362 379 581
Fragments arranged by size:
1362 581 379
AlwNI CAG_nnn'CTG
Cuts at: 0 67 169 2322
Size: 67 102 2153
Fragments arranged by size:
2153 102 67
ApaLI G'TGCA_C
Cuts at: 0 1167 2322
Size: 1167 1155
ApoI r'AATT_y
Cuts at: 0 403 1227 2169 2322
Size: 403 824 942 153
Fragments arranged by size:
942 824 403 153
BanI G'GyrC_C
Cuts at: 0 144 2322
Size: 144 2178
BanII G_rGCy'C
Cuts at: 0 1249 2322
Size: 1249 1073
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 2322
Size: 269 2053
BbvCI CC'TCA_GC
Cuts at: 0 173 1794 2322
Size: 173 1621 528
Fragments arranged by size:
1621 528 173
BclI T'GATC_A
Cuts at: 0 179 2322
Size: 179 2143
BfrBI ATG'CAT
Cuts at: 0 1485 2322
Size: 1485 837
Bme1580I G_kGCm'C
Cuts at: 0 1171 2322
Size: 1171 1151
BmgBI CAC'GTC
Cuts at: 0 1704 2322
Size: 1704 618
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 2162 2322
Size: 978 1184 160
Fragments arranged by size:
1184 978 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1953 2322
Size: 1953 369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 142 193 722 1263 1600 1647 2194
Size: 91 51 51 529 541 337 47 547
Cuts at: 2194 2322
Size: 128
Fragments arranged by size:
547 541 529 337 128 91 51 51
47
Bpu10I CC'TnA_GC
Cuts at: 0 173 1102 1264 1794 2322
Size: 173 929 162 530 528
Fragments arranged by size:
929 530 528 173 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 405 500 1770 2322
Size: 405 95 1270 552
Fragments arranged by size:
1270 552 405 95
BsaAI yAC'GTr
Cuts at: 0 953 2322
Size: 953 1369
BsaBI GATnn'nnATC
Cuts at: 0 323 1973 2322
Size: 323 1650 349
Fragments arranged by size:
1650 349 323
BsaHI Gr'CG_yC
Cuts at: 0 1617 2322
Size: 1617 705
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 191 221 665 695 2322
Size: 86 30 75 30 444 30 1627
Fragments arranged by size:
1627 444 86 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1394 2322
Size: 94 1300 928
Fragments arranged by size:
1300 928 94
BseYI C'CCAG_C
Cuts at: 0 336 560 889 2322
Size: 336 224 329 1433
Fragments arranged by size:
1433 336 329 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1747 2322
Size: 55 1692 575
Fragments arranged by size:
1692 575 55
BsiHKAI G_wGCw'C
Cuts at: 0 528 1171 2322
Size: 528 643 1151
Fragments arranged by size:
1151 643 528
BsmI GAATG_Cn'
Cuts at: 0 351 2322
Size: 351 1971
Bsp1286I G_dGCh'C
Cuts at: 0 528 1171 1249 2322
Size: 528 643 78 1073
Fragments arranged by size:
1073 643 528 78
BspHI T'CATG_A
Cuts at: 0 6 2322
Size: 6 2316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 721 1140 2322
Size: 615 106 419 1182
Fragments arranged by size:
1182 615 419 106
BsrDI GCAATG_nn'
Cuts at: 0 20 2322
Size: 20 2302
BsrFI r'CCGG_y
Cuts at: 0 899 2322
Size: 899 1423
BsrGI T'GTAC_A
Cuts at: 0 859 2058 2322
Size: 859 1199 264
Fragments arranged by size:
1199 859 264
BssSI C'ACGA_G
Cuts at: 0 1713 2322
Size: 1713 609
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2173 2322
Size: 2173 149
BstYI r'GATC_y
Cuts at: 0 313 1496 2322
Size: 313 1183 826
Fragments arranged by size:
1183 826 313
Bsu36I CC'TnA_GG
Cuts at: 0 1406 2322
Size: 1406 916
BtgI C'CryG_G
Cuts at: 0 1500 1592 2322
Size: 1500 92 730
Fragments arranged by size:
1500 730 92
ClaI AT'CG_AT
Cuts at: 0 764 2322
Size: 764 1558
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1574 1609 2322
Size: 1574 35 713
Fragments arranged by size:
1574 713 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1018 2322
Size: 1018 1304
EaeI y'GGCC_r
Cuts at: 0 77 1676 2322
Size: 77 1599 646
Fragments arranged by size:
1599 646 77
EarI CTCTTCn'nnn_
Cuts at: 0 611 713 1456 2322
Size: 611 102 743 866
Fragments arranged by size:
866 743 611 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 142 193 278 635 722 1108 1263
Size: 91 51 51 85 357 87 386 155
Cuts at: 1263 1600 1647 2194 2322
Size: 337 47 547 128
Fragments arranged by size:
547 386 357 337 155 128 91 87
85 51 51 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1087 2322
Size: 1087 1235
EcoO109I rG'GnC_Cy
Cuts at: 0 557 1152 1260 2077 2214 2322
Size: 557 595 108 817 137 108
Fragments arranged by size:
817 595 557 137 108 108
EcoRI G'AATT_C
Cuts at: 0 403 2322
Size: 403 1919
EcoRV GAT'ATC
Cuts at: 0 1391 2322
Size: 1391 931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1326 2322
Size: 1326 996
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 588 620 1108 1140 1607 1639
Size: 86 32 470 32 488 32 467 32
Cuts at: 1639 2322
Size: 683
Fragments arranged by size:
683 488 470 467 86 32 32 32
32
HincII GTy'rAC
Cuts at: 0 234 684 2322
Size: 234 450 1638
Fragments arranged by size:
1638 450 234
KpnI G_GTAC'C
Cuts at: 0 148 2322
Size: 148 2174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 2219 2322
Size: 1167 1052 103
Fragments arranged by size:
1167 1052 103
MscI TGG'CCA
Cuts at: 0 79 1678 2322
Size: 79 1599 644
Fragments arranged by size:
1599 644 79
MslI CAynn'nnrTG
Cuts at: 0 1480 2143 2322
Size: 1480 663 179
Fragments arranged by size:
1480 663 179
MspA1I CmG'CkG
Cuts at: 0 374 1798 2322
Size: 374 1424 524
Fragments arranged by size:
1424 524 374
NdeI CA'TA_TG
Cuts at: 0 1809 2322
Size: 1809 513
NsiI A_TGCA'T
Cuts at: 0 1487 2322
Size: 1487 835
NspI r_CATG'y
Cuts at: 0 399 761 1782 2322
Size: 399 362 1021 540
Fragments arranged by size:
1021 540 399 362
PasI CC'CwG_GG
Cuts at: 0 1241 2322
Size: 1241 1081
PciI A'CATG_T
Cuts at: 0 757 2322
Size: 757 1565
PflMI CCAn_nnn'nTGG
Cuts at: 0 1429 1813 2322
Size: 1429 384 509
Fragments arranged by size:
1429 509 384
PfoI T'CCnGG_A
Cuts at: 0 2210 2322
Size: 2210 112
PmlI CAC'GTG
Cuts at: 0 953 2322
Size: 953 1369
PpuMI rG'GwC_Cy
Cuts at: 0 557 1152 1260 2077 2214 2322
Size: 557 595 108 817 137 108
Fragments arranged by size:
817 595 557 137 108 108
PsiI TTA'TAA
Cuts at: 0 1607 2322
Size: 1607 715
PstI C_TGCA'G
Cuts at: 0 383 666 1151 2322
Size: 383 283 485 1171
Fragments arranged by size:
1171 485 383 283
PvuII CAG'CTG
Cuts at: 0 1798 2322
Size: 1798 524
SapI GCTCTTCn'nnn_
Cuts at: 0 713 1456 2322
Size: 713 743 866
Fragments arranged by size:
866 743 713
ScaI AGT'ACT
Cuts at: 0 1449 2322
Size: 1449 873
SfcI C'TryA_G
Cuts at: 0 379 662 1147 1959 2218 2322
Size: 379 283 485 812 259 104
Fragments arranged by size:
812 485 379 283 259 104
SpeI A'CTAG_T
Cuts at: 0 1054 2322
Size: 1054 1268
SphI G_CATG'C
Cuts at: 0 399 2322
Size: 399 1923
StuI AGG'CCT
Cuts at: 0 1411 1965 2322
Size: 1411 554 357
Fragments arranged by size:
1411 554 357
StyI C'CwwG_G
Cuts at: 0 87 1832 1895 2322
Size: 87 1745 63 427
Fragments arranged by size:
1745 427 87 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2239 2322
Size: 2239 83
TatI w'GTAC_w
Cuts at: 0 859 1447 1472 2058 2149 2322
Size: 859 588 25 586 91 173
Fragments arranged by size:
859 588 586 173 91 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 495 527 2019 2051 2322
Size: 191 32 272 32 1492 32 271
Fragments arranged by size:
1492 272 271 191 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1015 2322
Size: 1015 1307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1758 2322
Size: 1758 564
ZraI GAC'GTC
Cuts at: 0 1618 2322
Size: 1618 704
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AlfI AlwNI
ApaLI ApoI BanI BanII BbsI BbvCI BclI BfrBI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstYI Bsu36I BtgI ClaI CspCI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
KpnI MmeI MscI MslI MspA1I NdeI NsiI NspI
PasI PciI PflMI PfoI PmlI PpuMI PsiI PstI
PvuII SapI ScaI SfcI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AleI AloI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BciVI BglI BglII BlpI BmtI BpuEI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstBI BstEII
BstXI BstZ17I BtgZI BtsI DraI DraIII EagI EciI
EcoICRI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PmeI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI
SspI SwaI XbaI XhoI XmaI XmnI