(Circular) (Six-base) MAPSORT of: pFN21AE3268.seq Check: 9923 from: 1 to: 6703 pFN21AE3268 6703 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4513 278 Size: 53 83 186 936 2977 2468 Fragments arranged by size: 2977 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3439 4046 1937 Size: 1502 607 4594 Fragments arranged by size: 4594 1502 607 Acc65I G'GTAC_C Cuts at: 4024 4024 Size: 6703 AclI AA'CG_TT Cuts at: 1563 3628 4830 5203 1563 Size: 2065 1202 373 3063 Fragments arranged by size: 3063 2065 1202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2955 3460 4766 6150 6418 1632 Size: 1323 505 1306 1384 268 1917 Fragments arranged by size: 1917 1384 1323 1306 505 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6506 AflIII A'CryG_T Cuts at: 1203 5602 6579 1203 Size: 4399 977 1327 Fragments arranged by size: 4399 1327 977 AgeI A'CCGG_T Cuts at: 5534 5534 Size: 6703 AhdI GACnn_n'nnGTC Cuts at: 3452 5432 3452 Size: 1980 4723 AleI CACnn'nnGTG Cuts at: 1166 2912 1166 Size: 1746 4957 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3368 1184 Size: 2184 4519 AlwNI CAG_nnn'CTG Cuts at: 2090 2822 4446 6018 2090 Size: 732 1624 1572 2775 Fragments arranged by size: 2775 1624 1572 732 ApaLI G'TGCA_C Cuts at: 4760 5916 4760 Size: 1156 5547 ApoI r'AATT_y Cuts at: 1493 2485 2536 2686 2737 3194 4012 4238 4390 Size: 992 51 150 51 457 818 226 152 Cuts at: 4390 6499 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 992 818 457 226 152 150 51 51 AseI AT'TA_AT Cuts at: 160 1032 5257 160 Size: 872 4225 1606 Fragments arranged by size: 4225 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6703 AvaI C'yCGr_G Cuts at: 1870 1943 4028 1870 Size: 73 2085 4545 Fragments arranged by size: 4545 2085 73 BamHI G'GATC_C Cuts at: 4033 4400 4033 Size: 367 6336 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2914 4024 5479 618 Size: 325 217 627 1127 1110 1455 1842 Fragments arranged by size: 1842 1455 1127 1110 627 325 217 BanII G_rGCy'C Cuts at: 1391 3162 4022 1391 Size: 1771 860 4072 Fragments arranged by size: 4072 1771 860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2361 2994 928 Size: 971 462 633 4637 Fragments arranged by size: 4637 971 633 462 BbvCI CC'TCA_GC Cuts at: 2586 2586 Size: 6703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4894 4928 1903 Size: 34 2957 34 3678 Fragments arranged by size: 3678 2957 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3267 4595 5805 3267 Size: 1328 1210 4165 Fragments arranged by size: 4165 1328 1210 BclI T'GATC_A Cuts at: 1548 2092 2140 3972 1548 Size: 544 48 1832 4279 Fragments arranged by size: 4279 1832 544 48 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5314 136 Size: 107 122 71 631 605 3642 1525 Fragments arranged by size: 3642 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6698 6698 Size: 6703 BlpI GC'TnA_GC Cuts at: 2052 3698 4120 2052 Size: 1646 422 4635 Fragments arranged by size: 4635 1646 422 Bme1580I G_kGCm'C Cuts at: 1790 2509 4764 5920 1790 Size: 719 2255 1156 2573 Fragments arranged by size: 2573 2255 1156 719 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6703 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4434 5392 442 Size: 416 53 83 399 24 3017 958 1753 Fragments arranged by size: 3017 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6703 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2988 5363 1239 Size: 1749 2375 2579 Fragments arranged by size: 2579 2375 1749 Bpu10I CC'TnA_GC Cuts at: 2586 2586 Size: 6703 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4828 5693 5991 4828 Size: 865 298 5540 Fragments arranged by size: 5540 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 5366 882 Size: 617 1563 2304 2219 Fragments arranged by size: 2304 2219 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 3647 4494 493 Size: 711 2443 847 2702 Fragments arranged by size: 2702 2443 847 711 BsaBI GATnn'nnATC Cuts at: 2091 2316 3560 6697 2091 Size: 225 1244 3137 2097 Fragments arranged by size: 3137 2097 1244 225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 4510 4892 5527 Size: 53 83 186 936 504 2473 382 635 Cuts at: 5527 275 Size: 1451 Fragments arranged by size: 2473 1451 936 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3914 5136 5534 5808 5955 6366 3914 Size: 1222 398 274 147 411 4251 Fragments arranged by size: 4251 1222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6478 Fragments arranged by size: 6478 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2591 2636 3068 3749 3752 3764 1186 Size: 1327 78 45 432 681 3 12 4125 Fragments arranged by size: 4125 1327 681 432 78 45 12 3 BseYI C'CCAG_C Cuts at: 5906 6402 5906 Size: 496 6207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2976 1163 Size: 573 1240 4890 Fragments arranged by size: 4890 1240 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2654 4153 4914 5942 1540 Size: 275 186 653 1499 761 1028 2301 Fragments arranged by size: 2301 1499 1028 761 653 275 186 BsiHKAI G_wGCw'C Cuts at: 4764 4849 5920 4764 Size: 85 1071 5547 Fragments arranged by size: 5547 1071 85 BsmI GAATG_Cn' Cuts at: 2107 2782 2878 3596 3795 4214 4307 2107 Size: 675 96 718 199 419 93 4503 Fragments arranged by size: 4503 718 675 419 199 96 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2509 3162 4022 4764 4849 5920 1391 Size: 399 719 653 860 742 85 1071 2174 Fragments arranged by size: 2174 1071 860 742 719 653 399 85 BspEI T'CCGG_A Cuts at: 3914 6366 3914 Size: 2452 4251 BspHI T'CATG_A Cuts at: 4592 6322 4592 Size: 1730 4973 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4058 844 Size: 3214 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4590 1394 Size: 429 2767 3507 Fragments arranged by size: 3507 2767 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3319 3370 5198 5372 65 Size: 1183 2071 51 1828 174 1396 Fragments arranged by size: 2071 1828 1396 1183 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 2653 5347 5534 1700 Size: 110 843 2694 187 2869 Fragments arranged by size: 2869 2694 843 187 110 BsrGI T'GTAC_A Cuts at: 96 3381 96 Size: 3285 3418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6703 BssSI C'ACGA_G Cuts at: 4763 5775 4763 Size: 1012 5691 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6703 BstBI TT'CG_AA Cuts at: 5507 5507 Size: 6703 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6703 BstXI CCAn_nnnn'nTGG Cuts at: 3712 3763 3712 Size: 51 6652 BstYI r'GATC_y Cuts at: 1972 2020 2075 2513 4033 4400 4785 4802 6254 Size: 48 55 438 1520 367 385 17 1452 Cuts at: 6254 6340 6352 6698 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1520 1452 438 385 367 346 86 55 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3036 3036 Size: 6703 BtgI C'CryG_G Cuts at: 513 1065 2004 2937 513 Size: 552 939 933 4279 Fragments arranged by size: 4279 939 933 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3993 487 Size: 683 56 2767 3197 Fragments arranged by size: 3197 2767 683 56 BtsI GCAGTG_nn' Cuts at: 2839 3392 4219 5013 5033 2839 Size: 553 827 794 20 4509 Fragments arranged by size: 4509 827 794 553 20 ClaI AT'CG_AT Cuts at: 1554 3724 1554 Size: 2170 4533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6668 DraI TTT'AAA Cuts at: 3460 4007 3460 Size: 547 6156 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5710 809 Size: 507 4394 1802 Fragments arranged by size: 4394 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2651 4150 5039 Size: 54 1425 50 275 18 821 1499 889 Cuts at: 5039 6668 8 Size: 1629 43 Fragments arranged by size: 1629 1499 1425 889 821 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2651 4150 1537 Size: 275 839 1499 4090 Fragments arranged by size: 4090 1499 839 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2718 2790 2974 3045 3978 4633 1353 Size: 677 688 72 184 71 933 655 3423 Fragments arranged by size: 3423 933 688 677 655 184 72 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5290 5664 5810 387 Size: 1064 138 435 3266 374 146 1280 Fragments arranged by size: 3266 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2955 2988 3460 4766 5363 6150 6418 Size: 393 1323 33 472 1306 597 787 268 Cuts at: 6418 1239 Size: 1524 Fragments arranged by size: 1524 1323 1306 787 597 472 393 268 33 EcoNI CCTnn'n_nnAGG Cuts at: 3003 3003 Size: 6703 EcoRI G'AATT_C Cuts at: 2536 2686 4012 4390 2536 Size: 150 1326 378 4849 Fragments arranged by size: 4849 1326 378 150 EcoRV GAT'ATC Cuts at: 2965 2965 Size: 6703 FspI TGC'GCA Cuts at: 5209 5209 Size: 6703 HaeII r_GCGC'y Cuts at: 1678 3667 3705 5850 1678 Size: 1989 38 2145 2531 Fragments arranged by size: 2531 2145 1989 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2228 2249 2260 Size: 32 135 32 488 32 136 21 11 Cuts at: 2260 2281 2798 2830 2951 2983 3438 3470 3801 Size: 21 517 32 121 32 455 32 331 Cuts at: 3801 3827 3833 3859 5344 5376 5418 5450 1373 Size: 26 6 26 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 517 488 455 331 136 135 121 42 32 32 32 32 32 32 32 32 26 26 21 21 11 6 HincII GTy'rAC Cuts at: 669 1657 1938 4047 4293 669 Size: 988 281 2109 246 3079 Fragments arranged by size: 3079 2109 988 281 246 HindIII A'AGCT_T Cuts at: 748 2271 748 Size: 1523 5180 HpaI GTT'AAC Cuts at: 4293 4293 Size: 6703 KpnI G_GTAC'C Cuts at: 4028 4028 Size: 6703 MfeI C'AATT_G Cuts at: 4302 4302 Size: 6703 MluI A'CGCG_T Cuts at: 6579 6579 Size: 6703 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5817 6001 1305 Size: 4512 184 2007 Fragments arranged by size: 4512 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4881 Fragments arranged by size: 4881 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2226 2424 2787 2912 3605 4661 5020 Size: 648 1060 198 363 125 693 1056 359 Cuts at: 5020 5179 518 Size: 159 2042 Fragments arranged by size: 2042 1060 1056 693 648 363 359 198 159 125 MspA1I CmG'CkG Cuts at: 1606 2713 3691 4119 4796 5944 6189 1606 Size: 1107 978 428 677 1148 245 2120 Fragments arranged by size: 2120 1148 1107 978 677 428 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2937 513 Size: 552 939 933 4279 Fragments arranged by size: 4279 939 933 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6703 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6703 NotI GC'GGCC_GC Cuts at: 4150 4150 Size: 6703 NspI r_CATG'y Cuts at: 1132 1229 3349 4061 5606 1132 Size: 97 2120 712 1545 2229 Fragments arranged by size: 2229 2120 1545 712 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6703 PciI A'CATG_T Cuts at: 5602 5602 Size: 6703 PflMI CCAn_nnn'nTGG Cuts at: 1108 3575 1108 Size: 2467 4236 PfoI T'CCnGG_A Cuts at: 3004 3004 Size: 6703 PmeI GTTT'AAAC Cuts at: 4007 4007 Size: 6703 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4772 4804 6285 6317 4772 Size: 32 1481 32 5158 Fragments arranged by size: 5158 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2361 1613 Size: 748 5955 PsiI TTA'TAA Cuts at: 3422 4273 3422 Size: 851 5852 PstI C_TGCA'G Cuts at: 830 2535 3399 4055 830 Size: 1705 864 656 3478 Fragments arranged by size: 3478 1705 864 656 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6703 PvuII CAG'CTG Cuts at: 2713 2713 Size: 6703 SalI G'TCGA_C Cuts at: 1936 4045 1936 Size: 2109 4594 SbfI CC_TGCA'GG Cuts at: 4055 4055 Size: 6703 ScaI AGT'ACT Cuts at: 4951 6663 4951 Size: 1712 4991 SexAI A'CCwGG_T Cuts at: 3233 3233 Size: 6703 SfcI C'TryA_G Cuts at: 826 1045 2531 3395 3440 4051 5186 5867 6058 Size: 219 1486 864 45 611 1135 681 191 Cuts at: 6058 826 Size: 1471 Fragments arranged by size: 1486 1471 1135 864 681 611 219 191 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6703 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6703 SmaI CCC'GGG Cuts at: 1872 4030 1872 Size: 2158 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4807 5708 5970 820 Size: 197 926 2864 901 262 1553 Fragments arranged by size: 2864 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3647 4494 493 Size: 3154 847 2702 Fragments arranged by size: 3154 2702 847 SpeI A'CTAG_T Cuts at: 152 152 Size: 6703 SphI G_CATG'C Cuts at: 1132 4061 1132 Size: 2929 3774 SspI AAT'ATT Cuts at: 5 52 4627 5 Size: 47 4575 2081 Fragments arranged by size: 4575 2081 47 StuI AGG'CCT Cuts at: 1517 2584 2762 1517 Size: 1067 178 5458 Fragments arranged by size: 5458 1067 178 StyI C'CwwG_G Cuts at: 513 1065 2004 2937 4142 513 Size: 552 939 933 1205 3074 Fragments arranged by size: 3074 1205 939 933 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4730 4747 4900 5085 5530 325 Size: 1255 3150 17 153 185 445 1498 Fragments arranged by size: 3150 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3381 4949 6661 Size: 274 80 33 51 1443 1404 1568 1712 Cuts at: 6661 96 Size: 138 Fragments arranged by size: 1712 1568 1443 1404 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6663 6695 6663 Size: 32 6671 Tth111I GACn'n_nGTC Cuts at: 1316 3520 1316 Size: 2204 4499 XbaI T'CTAG_A Cuts at: 4039 4039 Size: 6703 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6703 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6703 XmaI C'CCGG_G Cuts at: 1870 4028 1870 Size: 2158 4545 XmnI GAAnn'nnTTC Cuts at: 3820 4832 3820 Size: 1012 5691 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4511 276 Size: 53 83 186 936 2977 2468 Fragments arranged by size: 2977 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI