################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AclI AflII AflIII Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BstAPI BstBI Bsu36I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoT22I FbaI HincII HindII HpaI KasI MaeI MamI MfeI MluI MunI NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 1062 GGTACC G^GTACC AccIII 1465 TCCGGA T^CCGGA AcyI 169 GACGCC G[GA]^CG[CT]C AgeI 1935 ACCGGT A^CCGGT AhdI 2020 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 801 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1062 GGTACC G^GTACC AspEI 2020 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 629 TGCGCA TGC^GCA BalI 767 TGGCCA TGG^CCA BbvCI 963 CCTCAGC CCTCAGC(-5/-2) BglI 229 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 126 GCTGAGC GC^T[ACGT]AGC Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC BsaAI 425 TACGTG [CT]AC^GT[GA] BsaHI 169 GACGCC G[GA]^CG[CT]C BsaI 847 GAGACC GGTCTC(1/5) BseAI 1465 TCCGGA T^CCGGA BsmBI 673 GAGACG CGTCTC(1/5) BspEI 1465 TCCGGA T^CCGGA BssHII 565 GCGCGC G^CGCGC BssSI 460 CTCGTG CACGAG(-5/-1) Bst1107I 1660 GTATAC GTA^TAC BstEII 2089 GGTCACC G^GT[ACGT]ACC BstPI 2089 GGTCACC G^GT[ACGT]ACC BstZ17I 1660 GTATAC GTA^TAC CelII 126 GCTGAGC GC^T[ACGT]AGC DraII 1113 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 591 CCGCGG C^C[GA][CT]GG Eam1105I 2020 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1113 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 2089 GGTCACC G^GT[ACGT]ACC EcoRI 1258 GAATTC G^AATTC EcoRV 1020 GATATC GAT^ATC EcoT14I 2052 CCTTGG C^C[AT][AT]GG FseI 595 GGCCGGCC GGCCGG^CC FspI 629 TGCGCA TGC^GCA HgaI 169 GACGC GACGC(5/10) Hin1I 169 GACGCC G[GA]^CG[CT]C HindIII 1056 AAGCTT A^AGCTT KpnI 1062 GGTACC GGTAC^C KspI 591 CCGCGG CCGC^GG MluNI 767 TGGCCA TGG^CCA MroI 1465 TCCGGA T^CCGGA MscI 767 TGGCCA TGG^CCA MslI 1350 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 979 CATATG CA^TATG PaeR7I 505 CTCGAG C^TCGAG PinAI 1935 ACCGGT A^CCGGT PpuMI 1113 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1959 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 591 CCGCGG CCGC^GG SapI 1031 GCTCTTC GCTCTTC(1/4) ScaI 1072 AGTACT AGT^ACT SgrAI 1934 CACCGGTG C[GA]^CCGG[CT]G StuI 1335 AGGCCT AGG^CCT StyI 2052 CCTTGG C^C[AT][AT]GG XcmI 1479 CCAGTTCCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 505 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspI 331 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 849 GACCCAGTC BsaWI 1465 TCCGGA [AT]^CCGG[AT] 1935 ACCGGT BsgI 1931 CTGCAC GTGCAG(16/14) 1652 GTGCAG BsmI 1758 GAATGC GAATGC(1/-1) 1370 GCATTC BspMI 419 ACCTGC ACCTGC(4/8) 1065 ACCTGC BsrBI 195 GAGCGG CCGCTC(-3/-3) 233 GAGCGG BsrDI 1908 CATTGC GCAATG(2/0) 1205 GCAATG BstXI 974 CCAGTCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1649 CCAGTGCAGTGG BstYI 1454 AGATCC [GA]^GATC[CT] 1693 GGATCT HaeII 70 AGCGCC [GA]GCGC^[CT] 456 GGCGCT MflI 1454 AGATCC [GA]^GATC[CT] 1693 GGATCT NaeI 596 GCCGGC GCC^GGC 821 GCCGGC NgoMIV 596 GCCGGC G^CCGGC 821 GCCGGC PflFI 331 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 849 GACCCAGTC TaqI 506 TCGA T^CGA 521 TCGA Tth111I 331 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 849 GACCCAGTC TthHB8I 506 TCGA T^CGA 521 TCGA XhoII 1454 AGATCC [GA]^GATC[CT] 1693 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EagI EarI EclXI Eco52I EcoNI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI