Restriction map for
pFN21AB6280
(Circular) (Six-base) MAPSORT of: pFN21AB6280.seq Check: 7794 from: 1 to: 7048
pFN21AB6280 7048 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4858 278
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2619 3242 3608 3666 4391 1937
Size: 682 623 366 58 725 4594
Fragments arranged by size:
4594 725 682 623 366 58
Acc65I G'GTAC_C
Cuts at: 3067 4369 3067
Size: 1302 5746
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2913 3564 3980 4101 5111 6495 6763
Size: 839 442 651 416 121 1010 1384 268
Cuts at: 6763 1632
Size: 1917
Fragments arranged by size:
1917 1384 1010 839 651 442 416 268
121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6851
AflIII A'CryG_T
Cuts at: 1203 5947 6924 1203
Size: 4744 977 1327
Fragments arranged by size:
4744 1327 977
AgeI A'CCGG_T
Cuts at: 3940 5879 3940
Size: 1939 5109
AhdI GACnn_n'nnGTC
Cuts at: 4030 5777 4030
Size: 1747 5301
AleI CACnn'nnGTG
Cuts at: 1166 3359 1166
Size: 2193 4855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AlwNI CAG_nnn'CTG
Cuts at: 2811 4791 6363 2811
Size: 1980 1572 3496
Fragments arranged by size:
3496 1980 1572
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 2642 3103 3263 4174 4186 4357 4583 4735
Size: 1149 461 160 911 12 171 226 152
Cuts at: 4735 6844 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1149 911 461 226 171 160
152 12
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 2492 2510 3802 4373 1870
Size: 73 549 18 1292 571 4545
Fragments arranged by size:
4545 1292 571 549 73 18
BamHI G'GATC_C
Cuts at: 4378 4745 4378
Size: 367 6681
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2283 2766 3067 4369 5824
Size: 325 217 627 496 483 301 1302 1455
Cuts at: 5824 618
Size: 1842
Fragments arranged by size:
1842 1455 1302 627 496 483 325 301
217
BanII G_rGCy'C
Cuts at: 1391 2138 2499 2596 2727 3644 4367 1391
Size: 747 361 97 131 917 723 4072
Fragments arranged by size:
4072 917 747 723 361 131 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3082 3780 4281 928
Size: 971 1183 698 501 3695
Fragments arranged by size:
3695 1183 971 698 501
BbvCI CC'TCA_GC
Cuts at: 2969 2969
Size: 7048
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5239 5273 1903
Size: 34 3302 34 3678
Fragments arranged by size:
3678 3302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4940 6150 4940
Size: 1210 5838
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7048
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 5659 136
Size: 107 122 71 631 605 568 3419 1525
Fragments arranged by size:
3419 1525 631 605 568 122 107 71
BglII A'GATC_T
Cuts at: 7043 7043
Size: 7048
BlpI GC'TnA_GC
Cuts at: 2132 4465 2132
Size: 2333 4715
Bme1580I G_kGCm'C
Cuts at: 1790 5109 6265 1790
Size: 3319 1156 2573
Fragments arranged by size:
3319 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2341 3256 3306 1318
Size: 1023 915 50 5060
Fragments arranged by size:
5060 1023 915 50
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2851 3117 3245
Size: 416 53 83 399 24 1434 266 128
Cuts at: 3245 3312 3648 4779 5737 442
Size: 67 336 1131 958 1753
Fragments arranged by size:
1753 1434 1131 958 416 399 336 266
128 83 67 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2183 2926 3063 3260 4295 5708 1239
Size: 891 53 743 137 197 1035 1413 2579
Fragments arranged by size:
2579 1413 1035 891 743 197 137 53
Bpu10I CC'TnA_GC
Cuts at: 2969 2969
Size: 7048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 3556 3658 5173 6038 6336 2702
Size: 854 102 1515 865 298 3414
Fragments arranged by size:
3414 1515 865 854 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2846 5711 882
Size: 617 1347 2865 2219
Fragments arranged by size:
2865 2219 1347 617
BsaAI yAC'GTr
Cuts at: 493 1204 2432 4839 493
Size: 711 1228 2407 2702
Fragments arranged by size:
2702 2407 1228 711
BsaBI GATnn'nnATC
Cuts at: 7042 7042
Size: 7048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 4855 5237 5872
Size: 53 83 186 936 642 2680 382 635
Cuts at: 5872 275
Size: 1451
Fragments arranged by size:
2680 1451 936 642 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3470 3940 5481 5879 6153 6300 6711 3470
Size: 470 1541 398 274 147 411 3807
Fragments arranged by size:
3807 1541 470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 2924 2954 3393
Size: 30 165 30 1266 30 225 30 439
Cuts at: 3393 3423 1178
Size: 30 4803
Fragments arranged by size:
4803 1266 439 225 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 2919 3820 1186
Size: 1504 229 901 4414
Fragments arranged by size:
4414 1504 901 229
BseYI C'CCAG_C
Cuts at: 2608 2885 3350 4105 6251 6747 2608
Size: 277 465 755 2146 496 2909
Fragments arranged by size:
2909 2146 755 496 465 277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3678 3921 1163
Size: 573 1942 243 4290
Fragments arranged by size:
4290 1942 573 243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2184 2235 2602 2606 4498 5259
Size: 275 186 183 51 367 4 1892 761
Cuts at: 5259 6287 1540
Size: 1028 2301
Fragments arranged by size:
2301 1892 1028 761 367 275 186 183
51 4
BsiHKAI G_wGCw'C
Cuts at: 2499 2727 3644 5109 5194 6265 2499
Size: 228 917 1465 85 1071 3282
Fragments arranged by size:
3282 1465 1071 917 228 85
BsmI GAATG_Cn'
Cuts at: 3375 3769 4559 4652 3375
Size: 394 790 93 5771
Fragments arranged by size:
5771 790 394 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 2672
Size: 7048
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2499 2596 2727 3644 4367 5109
Size: 399 348 361 97 131 917 723 742
Cuts at: 5109 5194 6265 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 917 742 723 399 361 348
131 97 85
BspEI T'CCGG_A
Cuts at: 3470 6711 3470
Size: 3241 3807
BspHI T'CATG_A
Cuts at: 4937 6667 4937
Size: 1730 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 3079 4403 844
Size: 1589 646 1324 3489
Fragments arranged by size:
3489 1589 1324 646
BsrBI CCG'CTC
Cuts at: 1394 1823 2202 2240 4935 1394
Size: 429 379 38 2695 3507
Fragments arranged by size:
3507 2695 429 379 38
BsrDI GCAATG_nn'
Cuts at: 65 1248 3217 3912 5543 5717 65
Size: 1183 1969 695 1631 174 1396
Fragments arranged by size:
1969 1631 1396 1183 695 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2601 2628 2783 2826 3940 5692 5879
Size: 110 791 27 155 43 1114 1752 187
Cuts at: 5879 1700
Size: 2869
Fragments arranged by size:
2869 1752 1114 791 187 155 110 43
27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7048
BssHII G'CGCG_C
Cuts at: 1925 2570 1925
Size: 645 6403
BssSI C'ACGA_G
Cuts at: 2465 5108 6120 2465
Size: 2643 1012 3393
Fragments arranged by size:
3393 2643 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7048
BstBI TT'CG_AA
Cuts at: 5852 5852
Size: 7048
BstEII G'GTnAC_C
Cuts at: 1184 4094 1184
Size: 2910 4138
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 3661 2986
Size: 675 6373
BstYI r'GATC_y
Cuts at: 1972 3459 3698 4378 4745 5130 5147 6599 6685
Size: 1487 239 680 367 385 17 1452 86
Cuts at: 6685 6697 7043 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1487 1452 680 385 367 346 239
86 17 12
BstZ17I GTA'TAC
Cuts at: 3667 3667
Size: 7048
BtgI C'CryG_G
Cuts at: 513 1065 2596 513
Size: 552 1531 4965
Fragments arranged by size:
4965 1531 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6309
Fragments arranged by size:
6309 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 2360 3174 3515 3666 4564 5358 5378 2223
Size: 137 814 341 151 898 794 20 3893
Fragments arranged by size:
3893 898 814 794 341 151 137 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7013
DraI TTT'AAA
Cuts at: 4352 4352
Size: 7048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2532 2599
Size: 54 1425 50 275 18 402 300 67
Cuts at: 2599 2603 2772 4495 5384 7013 8
Size: 4 169 1723 889 1629 43
Fragments arranged by size:
1723 1629 1425 889 402 300 275 169
67 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2232 2599 2603 4495 1537
Size: 275 420 367 4 1892 4090
Fragments arranged by size:
4090 1892 420 367 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2017 3043 3999 4978 1353
Size: 664 1026 956 979 3423
Fragments arranged by size:
3423 1026 979 956 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 2261 5635 6009 6155 387
Size: 1064 138 471 201 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 471 374 201 146 138
EcoICRI GAG'CTC
Cuts at: 2497 2725 3642 2497
Size: 228 917 5903
Fragments arranged by size:
5903 917 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2183 2471 2913 2926 3063 3260
Size: 393 498 53 288 442 13 137 197
Cuts at: 3260 3564 3980 4101 4295 5111 5708 6495 6763
Size: 304 416 121 194 816 597 787 268
Cuts at: 6763 1239
Size: 1524
Fragments arranged by size:
1524 816 787 597 498 442 416 393
304 288 268 197 194 137 121 53
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2450 2798 3681 2450
Size: 348 883 5817
Fragments arranged by size:
5817 883 348
EcoO109I rG'GnC_Cy
Cuts at: 3119 3119
Size: 7048
EcoRI G'AATT_C
Cuts at: 3263 4357 4735 3263
Size: 1094 378 5576
Fragments arranged by size:
5576 1094 378
EcoRV GAT'ATC
Cuts at: 3027 3027
Size: 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3652 4261 3652
Size: 609 6439
FseI GG_CCGG'CC
Cuts at: 2605 2605
Size: 7048
FspI TGC'GCA
Cuts at: 2636 5554 2636
Size: 2918 4130
HaeII r_GCGC'y
Cuts at: 1678 2079 2465 6195 1678
Size: 401 386 3730 2531
Fragments arranged by size:
3730 2531 401 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 2909 2941 3389
Size: 32 135 32 1271 32 34 32 448
Cuts at: 3389 3421 4016 4048 4217 4249 5689 5721 5763
Size: 32 595 32 169 32 1440 32 42
Cuts at: 5763 5795 1373
Size: 32 2626
Fragments arranged by size:
2626 1440 1271 595 448 169 135 42
34 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4392 4638 669
Size: 988 281 2454 246 3079
Fragments arranged by size:
3079 2454 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3061 748
Size: 2313 4735
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KpnI G_GTAC'C
Cuts at: 3071 4373 3071
Size: 1302 5746
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3875 6162 6346 1305
Size: 2570 2287 184 2007
Fragments arranged by size:
2570 2287 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2774 10
Size: 54 1425 343 942 4284
Fragments arranged by size:
4284 1425 942 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 5006 5365 5524 518
Size: 648 2193 1647 359 159 2042
Fragments arranged by size:
2193 2042 1647 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 2320 2545 2598 2608 3523 4079 4324
Size: 580 134 225 53 10 915 556 245
Cuts at: 4324 4464 5141 6289 6534 1606
Size: 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 580 556 245 245
225 140 134 53 10
NaeI GCC'GGC
Cuts at: 2603 2828 2603
Size: 225 6823
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6496
NdeI CA'TA_TG
Cuts at: 387 2985 387
Size: 2598 4450
NgoMIV G'CCGG_C
Cuts at: 2601 2826 2601
Size: 225 6823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NspI r_CATG'y
Cuts at: 1132 1229 4406 5951 1132
Size: 97 3177 1545 2229
Fragments arranged by size:
3177 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7048
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3119 3119
Size: 7048
PshAI GACnn'nnGTC
Cuts at: 1613 3968 1613
Size: 2355 4693
PsiI TTA'TAA
Cuts at: 4618 4618
Size: 7048
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3657 3689 3657
Size: 32 7016
PstI C_TGCA'G
Cuts at: 830 2367 2553 3522 4400 830
Size: 1537 186 969 878 3478
Fragments arranged by size:
3478 1537 969 878 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 3523 4079 4324 3523
Size: 556 245 6247
Fragments arranged by size:
6247 556 245
SacI G_AGCT'C
Cuts at: 2499 2727 3644 2499
Size: 228 917 5903
Fragments arranged by size:
5903 917 228
SacII CC_GC'GG
Cuts at: 2599 2599
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SanDI GG'GwC_CC
Cuts at: 3119 3119
Size: 7048
SapI GCTCTTCn'nnn_
Cuts at: 3043 3043
Size: 7048
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 3079 5296 7008 3079
Size: 2217 1712 3119
Fragments arranged by size:
3119 2217 1712
SfcI C'TryA_G
Cuts at: 826 1045 2363 2500 2549 3310 3408 3518 3693
Size: 219 1318 137 49 761 98 110 175
Cuts at: 3693 4074 4396 5531 6212 6403 826
Size: 381 322 1135 681 191 1471
Fragments arranged by size:
1471 1318 1135 761 681 381 322 219
191 175 137 110 98 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 3940 1700
Size: 2240 4808
SmaI CCC'GGG
Cuts at: 1872 4375 1872
Size: 2503 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 2717 3571 3637 5152 6053
Size: 197 926 567 207 854 66 1515 901
Cuts at: 6053 6315 820
Size: 262 1553
Fragments arranged by size:
1553 1515 926 901 854 567 262 207
197 66
SnaBI TAC'GTA
Cuts at: 493 4839 493
Size: 4346 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 4406 1132
Size: 3274 3774
SspI AAT'ATT
Cuts at: 5 52 4972 5
Size: 47 4920 2081
Fragments arranged by size:
4920 2081 47
StuI AGG'CCT
Cuts at: 1517 3342 1517
Size: 1825 5223
StyI C'CwwG_G
Cuts at: 513 1065 4057 4487 513
Size: 552 2992 430 3074
Fragments arranged by size:
3074 2992 552 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2813 2949 5075 5092 5245 5430 5875
Size: 1255 1233 136 2126 17 153 185 445
Cuts at: 5875 325
Size: 1498
Fragments arranged by size:
2126 1498 1255 1233 445 185 153 136
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3077 4111 5294
Size: 274 80 33 51 1443 1100 1034 1183
Cuts at: 5294 7006 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1183 1100 1034 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2924 2956 3075 3107 3135 3167 7008 7040 2924
Size: 32 119 32 28 32 3841 32 2932
Fragments arranged by size:
3841 2932 119 32 32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 1316 2339 2857 1316
Size: 1023 518 5507
Fragments arranged by size:
5507 1023 518
XbaI T'CTAG_A
Cuts at: 4384 4384
Size: 7048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3491 3491
Size: 7048
XhoI C'TCGA_G
Cuts at: 1943 2510 1943
Size: 567 6481
XmaI C'CCGG_G
Cuts at: 1870 4373 1870
Size: 2503 4545
XmnI GAAnn'nnTTC
Cuts at: 3263 3375 4068 5177 3263
Size: 112 693 1109 5134
Fragments arranged by size:
5134 1109 693 112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4856 276
Size: 53 83 186 936 3322 2468
Fragments arranged by size:
3322 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI Bsu36I DraIII FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII
SexAI SfiI SfoI SrfI SwaI