(Linear) (Six-base) MAPSORT of: KIBB6280.seq Check: 8840 from: 1 to: 2343 KIBB6280.orf With 182 enzymes: SgfI * July 3, 2012 16:19 .. AccI GT'mk_AC Cuts at: 0 614 1237 1603 1661 2343 Size: 614 623 366 58 682 Fragments arranged by size: 682 623 614 366 58 Acc65I G'GTAC_C Cuts at: 0 1062 2343 Size: 1062 1281 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 908 1559 1975 2096 2343 Size: 466 442 651 416 121 247 Fragments arranged by size: 651 466 442 416 247 121 AgeI A'CCGG_T Cuts at: 0 1935 2343 Size: 1935 408 AhdI GACnn_n'nnGTC Cuts at: 0 2025 2343 Size: 2025 318 AleI CACnn'nnGTG Cuts at: 0 1354 2343 Size: 1354 989 AlwNI CAG_nnn'CTG Cuts at: 0 806 2343 Size: 806 1537 ApoI r'AATT_y Cuts at: 0 637 1098 1258 2169 2181 2343 Size: 637 461 160 911 12 162 Fragments arranged by size: 911 637 461 162 160 12 AvaI C'yCGr_G Cuts at: 0 487 505 1797 2343 Size: 487 18 1292 546 Fragments arranged by size: 1292 546 487 18 BanI G'GyrC_C Cuts at: 0 278 761 1062 2343 Size: 278 483 301 1281 Fragments arranged by size: 1281 483 301 278 BanII G_rGCy'C Cuts at: 0 133 494 591 722 1639 2343 Size: 133 361 97 131 917 704 Fragments arranged by size: 917 704 361 133 131 97 BbsI GAAGACnn'nnnn_ Cuts at: 0 1077 1775 2276 2343 Size: 1077 698 501 67 Fragments arranged by size: 1077 698 501 67 BbvCI CC'TCA_GC Cuts at: 0 964 2343 Size: 964 1379 BglI GCCn_nnn'nGGC Cuts at: 0 235 2343 Size: 235 2108 BlpI GC'TnA_GC Cuts at: 0 127 2343 Size: 127 2216 BmgBI CAC'GTC Cuts at: 0 336 1251 1301 2343 Size: 336 915 50 1042 Fragments arranged by size: 1042 915 336 50 BmrI ACTGGGnnnn_n' Cuts at: 0 846 1112 1240 1307 1643 2343 Size: 846 266 128 67 336 700 Fragments arranged by size: 846 700 336 266 128 67 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 178 921 1058 1255 2290 2343 Size: 125 53 743 137 197 1035 53 Fragments arranged by size: 1035 743 197 137 125 53 53 Bpu10I CC'TnA_GC Cuts at: 0 964 2343 Size: 964 1379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 697 1551 1653 2343 Size: 697 854 102 690 Fragments arranged by size: 854 697 690 102 BsaI GGTCTCn'nnnn_ Cuts at: 0 841 2343 Size: 841 1502 BsaAI yAC'GTr Cuts at: 0 427 2343 Size: 427 1916 BsaHI Gr'CG_yC Cuts at: 0 170 2343 Size: 170 2173 BsaWI w'CCGG_w Cuts at: 0 1465 1935 2343 Size: 1465 470 408 Fragments arranged by size: 1465 470 408 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 664 694 919 949 1388 1418 2343 Size: 664 30 225 30 439 30 925 Fragments arranged by size: 925 664 439 225 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 685 914 1815 2343 Size: 685 229 901 528 Fragments arranged by size: 901 685 528 229 BseYI C'CCAG_C Cuts at: 0 603 880 1345 2100 2343 Size: 603 277 465 755 243 Fragments arranged by size: 755 603 465 277 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1673 1916 2343 Size: 1673 243 427 Fragments arranged by size: 1673 427 243 BsiEI CG_ry'CG Cuts at: 0 179 230 597 601 2343 Size: 179 51 367 4 1742 Fragments arranged by size: 1742 367 179 51 4 BsiHKAI G_wGCw'C Cuts at: 0 494 722 1639 2343 Size: 494 228 917 704 Fragments arranged by size: 917 704 494 228 BsmI GAATG_Cn' Cuts at: 0 1370 1764 2343 Size: 1370 394 579 Fragments arranged by size: 1370 579 394 BsmBI CGTCTCn'nnnn_ Cuts at: 0 667 2343 Size: 667 1676 Bsp1286I G_dGCh'C Cuts at: 0 133 494 591 722 1639 2343 Size: 133 361 97 131 917 704 Fragments arranged by size: 917 704 361 133 131 97 BspEI T'CCGG_A Cuts at: 0 1465 2343 Size: 1465 878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 428 1074 2343 Size: 428 646 1269 Fragments arranged by size: 1269 646 428 BsrBI CCG'CTC Cuts at: 0 197 235 2343 Size: 197 38 2108 Fragments arranged by size: 2108 197 38 BsrDI GCAATG_nn' Cuts at: 0 1212 1907 2343 Size: 1212 695 436 Fragments arranged by size: 1212 695 436 BsrFI r'CCGG_y Cuts at: 0 596 623 778 821 1935 2343 Size: 596 27 155 43 1114 408 Fragments arranged by size: 1114 596 408 155 43 27 BssHII G'CGCG_C Cuts at: 0 565 2343 Size: 565 1778 BssSI C'ACGA_G Cuts at: 0 460 2343 Size: 460 1883 BstEII G'GTnAC_C Cuts at: 0 2089 2343 Size: 2089 254 BstXI CCAn_nnnn'nTGG Cuts at: 0 981 1656 2343 Size: 981 675 687 Fragments arranged by size: 981 687 675 BstYI r'GATC_y Cuts at: 0 1454 1693 2343 Size: 1454 239 650 Fragments arranged by size: 1454 650 239 BstZ17I GTA'TAC Cuts at: 0 1662 2343 Size: 1662 681 BtgI C'CryG_G Cuts at: 0 591 2343 Size: 591 1752 BtsI GCAGTG_nn' Cuts at: 0 218 355 1169 1510 1661 2343 Size: 218 137 814 341 151 682 Fragments arranged by size: 814 682 341 218 151 137 EaeI y'GGCC_r Cuts at: 0 227 527 594 598 767 2343 Size: 227 300 67 4 169 1576 Fragments arranged by size: 1576 300 227 169 67 4 EagI C'GGCC_G Cuts at: 0 227 594 598 2343 Size: 227 367 4 1745 Fragments arranged by size: 1745 367 227 4 EarI CTCTTCn'nnn_ Cuts at: 0 12 1038 1994 2343 Size: 12 1026 956 349 Fragments arranged by size: 1026 956 349 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 55 256 2343 Size: 55 201 2087 Fragments arranged by size: 2087 201 55 EcoICRI GAG'CTC Cuts at: 0 492 720 1637 2343 Size: 492 228 917 706 Fragments arranged by size: 917 706 492 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 178 466 908 921 1058 1255 1559 Size: 125 53 288 442 13 137 197 304 Cuts at: 1559 1975 2096 2290 2343 Size: 416 121 194 53 Fragments arranged by size: 442 416 304 288 197 194 137 125 121 53 53 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 445 793 1676 2343 Size: 445 348 883 667 Fragments arranged by size: 883 667 445 348 EcoO109I rG'GnC_Cy Cuts at: 0 1114 2343 Size: 1114 1229 EcoRI G'AATT_C Cuts at: 0 1258 2343 Size: 1258 1085 EcoRV GAT'ATC Cuts at: 0 1022 2343 Size: 1022 1321 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1647 2256 2343 Size: 1647 609 87 Fragments arranged by size: 1647 609 87 FseI GG_CCGG'CC Cuts at: 0 600 2343 Size: 600 1743 FspI TGC'GCA Cuts at: 0 631 2343 Size: 631 1712 HaeII r_GCGC'y Cuts at: 0 74 460 2343 Size: 74 386 1883 Fragments arranged by size: 1883 386 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 838 870 904 936 1384 1416 2011 2043 Size: 838 32 34 32 448 32 595 32 Cuts at: 2043 2212 2244 2343 Size: 169 32 99 Fragments arranged by size: 838 595 448 169 99 34 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1056 2343 Size: 1056 1287 KpnI G_GTAC'C Cuts at: 0 1066 2343 Size: 1066 1277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1870 2343 Size: 1870 473 MscI TGG'CCA Cuts at: 0 769 2343 Size: 769 1574 MslI CAynn'nnrTG Cuts at: 0 1354 2343 Size: 1354 989 MspA1I CmG'CkG Cuts at: 0 181 315 540 593 603 1518 2074 2319 Size: 181 134 225 53 10 915 556 245 Cuts at: 2319 2343 Size: 24 Fragments arranged by size: 915 556 245 225 181 134 53 24 10 NaeI GCC'GGC Cuts at: 0 598 823 2343 Size: 598 225 1520 Fragments arranged by size: 1520 598 225 NdeI CA'TA_TG Cuts at: 0 980 2343 Size: 980 1363 NgoMIV G'CCGG_C Cuts at: 0 596 821 2343 Size: 596 225 1522 Fragments arranged by size: 1522 596 225 PpuMI rG'GwC_Cy Cuts at: 0 1114 2343 Size: 1114 1229 PshAI GACnn'nnGTC Cuts at: 0 1963 2343 Size: 1963 380 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1652 1684 2343 Size: 1652 32 659 Fragments arranged by size: 1652 659 32 PstI C_TGCA'G Cuts at: 0 362 548 1517 2343 Size: 362 186 969 826 Fragments arranged by size: 969 826 362 186 PvuII CAG'CTG Cuts at: 0 1518 2074 2319 2343 Size: 1518 556 245 24 Fragments arranged by size: 1518 556 245 24 SacI G_AGCT'C Cuts at: 0 494 722 1639 2343 Size: 494 228 917 704 Fragments arranged by size: 917 704 494 228 SacII CC_GC'GG Cuts at: 0 594 2343 Size: 594 1749 SanDI GG'GwC_CC Cuts at: 0 1114 2343 Size: 1114 1229 SapI GCTCTTCn'nnn_ Cuts at: 0 1038 2343 Size: 1038 1305 ScaI AGT'ACT Cuts at: 0 1074 2343 Size: 1074 1269 SfcI C'TryA_G Cuts at: 0 358 495 544 1305 1403 1513 1688 2069 Size: 358 137 49 761 98 110 175 381 Cuts at: 2069 2343 Size: 274 Fragments arranged by size: 761 381 358 274 175 137 110 98 49 SgrAI Cr'CCGG_yG Cuts at: 0 1935 2343 Size: 1935 408 SmlI C'TyrA_G Cuts at: 0 505 712 1566 1632 2343 Size: 505 207 854 66 711 Fragments arranged by size: 854 711 505 207 66 StuI AGG'CCT Cuts at: 0 1337 2343 Size: 1337 1006 StyI C'CwwG_G Cuts at: 0 2052 2343 Size: 2052 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 808 944 2343 Size: 808 136 1399 Fragments arranged by size: 1399 808 136 TatI w'GTAC_w Cuts at: 0 1072 2106 2343 Size: 1072 1034 237 Fragments arranged by size: 1072 1034 237 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 919 951 1070 1102 1130 1162 2343 Size: 919 32 119 32 28 32 1181 Fragments arranged by size: 1181 919 119 32 32 32 28 Tth111I GACn'n_nGTC Cuts at: 0 334 852 2343 Size: 334 518 1491 Fragments arranged by size: 1491 518 334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1486 2343 Size: 1486 857 XhoI C'TCGA_G Cuts at: 0 505 2343 Size: 505 1838 XmnI GAAnn'nnTTC Cuts at: 0 1258 1370 2063 2343 Size: 1258 112 693 280 Fragments arranged by size: 1258 693 280 112 Enzymes that do cut: AccI Acc65I AcuI AgeI AhdI AleI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BglI BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BstYI BstZ17I BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV PpuMI PshAI PsrI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SgrAI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII Bme1580I BmtI BplI BsaBI BsiWI BspHI BsrGI BstAPI BstBI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI FspAI HincII HpaI KasI MfeI MluI NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SalI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI ZraI