################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluNI MroI MscI MseI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 248 GGTACC G^GTACC AccI 190 GTCTAC GT^[AC][GT]AC AccII 611 CGCG CG^CG AcsI 51 GAATTT [GA]^AATT[CT] AfaI 249 GTAC GT^AC AhdI 553 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 51 GAATTT [GA]^AATT[CT] Asp718I 248 GGTACC G^GTACC AspEI 553 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 254 GGACC G^G[AT]CC BanI 248 GGTACC G^G[CT][GA]CC BanII 594 GGGCTC G[GA]GC[CT]^C BbsI 183 GAAGAC GAAGAC(2/6) BglII 221 AGATCT A^GATCT BmyI 594 GGGCTC G[AGT]GC[ACT]^C BpmI 26 CTGGAG CTGGAG(16/14) BpuAI 183 GAAGAC GAAGAC(2/6) BsaWI 251 ACCGGA [AT]^CCGG[AT] BseRI 597 CTCCTC GAGGAG(10/8) BsmAI 614 GTCTC GTCTC(1/5) BsmBI 613 CGTCTC CGTCTC(1/5) Bsp1286I 594 GGGCTC G[AGT]GC[ACT]^C BspLU11I 62 ACATGT A^CATGT BsrBI 244 GAGCGG CCGCTC(-3/-3) BssSI 199 CACGAG CACGAG(-5/-1) BstF5I 540 GGATG GGATG(2/0) BstUI 611 CGCG CG^CG BstYI 221 AGATCT [GA]^GATC[CT] CfoI 531 GCGC GCG^C Eam1105I 553 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 284 CCATGG C^C[AT][AT]GG FauI 207 GCGGG CCCGC(4/6) FokI 540 GGATG GGATG(9/13) HaeII 530 AGCGCC [GA]GCGC^[CT] HapII 252 CCGG C^CGG HgaI 612 GCGTC GACGC(5/10) HhaI 531 GCGC GCG^C HinP1I 531 GCGC G^CGC HpaII 252 CCGG C^CGG KpnI 248 GGTACC GGTAC^C MaeII 136 ACGT A^CGT MflI 221 AGATCT [GA]^GATC[CT] MluI 610 ACGCGT A^CGCGT MslI 294 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 252 CCGG C^CGG MvnI 611 CGCG CG^CG NcoI 284 CCATGG C^CATGG NspI 62 ACATGT [GA]CATG^[CT] PvuII 233 CAGCTG CAG^CTG RsaI 249 GTAC GT^AC StuI 521 AGGCCT AGG^CCT StyI 284 CCATGG C^C[AT][AT]GG TfiI 565 GATTC G^A[AT]TC Tsp509I 52 AATT ^AATT XhoII 221 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BsiYI 339 CCAAGACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 365 CCTACTCCTGG BslI 339 CCAAGACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 365 CCTACTCCTGG BsmFI 227 GGGAC GGGAC(10/14) 374 GGGAC BssKI 371 CCTGG ^CC[ACGT]GG 534 CCTGG BstNI 371 CCTGG CC^[AT]GG 534 CCTGG Cac8I 449 GCCAGC GC[ACGT]^[ACGT]GC 519 GCAGGC DdeI 353 CTCAG C^T[ACGT]AG 440 CTGAG DpnII 222 GATC ^GATC 357 GATC EcoRII 371 CCTGG ^CC[AT]GG 534 CCTGG MboI 222 GATC ^GATC 357 GATC MspA1I 233 CAGCTG C[AC]G^C[GT]G 631 CCGCTG MvaI 371 CCTGG CC^[AT]GG 534 CCTGG NdeII 222 GATC ^GATC 357 GATC PleI 29 GAGTC GAGTC(4/5) 322 GAGTC PstI 217 CTGCAG CTGCA^G 637 CTGCAG Sau3AI 222 GATC ^GATC 357 GATC ScrFI 371 CCTGG CC^[ACGT]GG 534 CCTGG SfcI 217 CTGCAG C^T[GA][CT]AG 637 CTGCAG TaqI 170 TCGA T^CGA 586 TCGA TthHB8I 170 TCGA T^CGA 586 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AflIII AluI BbvI BsaJI BsrI Cfr13I DsaI EarI Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MwoI NlaIII NlaIV Sau96I SfaNI TseI Tsp45I TspRI