Restriction map for
pFN21AE3769
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI EclXI Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 1603 GTCTAC GT^[AC][GT]AC
AccII 88 CGCG CG^CG
AclI 2336 AACGTT AA^CGTT
AcyI 958 GACGCC G[GA]^CG[CT]C
AhdI 2296 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1653 AGCGCT AGC^GCT
Asp700I 510 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2296 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2206 GAGCTC G[AT]GC[AT]^C
AspI 2374 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1532 CTCGGG C^[CT]CG[GA]G
BanI 969 GGCACC G^G[CT][GA]CC
BbsI 2175 GAAGAC GAAGAC(2/6)
BcgI 2454 CGACCTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1203 GGATAC GTATCC(6/5)
BcnI 1089 CCGGG CC^[GC]GG
BglI 961 GCCCATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1080 AGATCT A^GATCT
BpuAI 2175 GAAGAC GAAGAC(2/6)
BsaHI 958 GACGCC G[GA]^CG[CT]C
BsaWI 30 ACCGGA [AT]^CCGG[AT]
BseRI 1007 CTCCTC GAGGAG(10/8)
BsgI 827 CTGCAC GTGCAG(16/14)
BsiEI 28 CGACCG CG[GA][CT]^CG
BsiHKAI 2206 GAGCTC G[AT]GC[AT]^C
BsoBI 1532 CTCGGG C^[CT]CG[GA]G
Bsp1407I 167 TGTACA T^GTACA
BspHI 794 TCATGA T^CATGA
BspMI 787 ACCTGC ACCTGC(4/8)
BsrFI 116 GCCGGT [GA]^CCGG[CT]
BsrGI 167 TGTACA T^GTACA
BstAPI 678 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 88 CGCG CG^CG
Cfr10I 116 GCCGGT [GA]^CCGG[CT]
DraII 1798 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2296 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1653 AGCGCT AGC^GCT
EcoO109I 1798 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2386 GAATTC G^AATTC
FauI 89 GCGGG CCCGC(4/6)
HaeII 1653 AGCGCT [GA]GCGC^[CT]
HgaI 958 GACGC GACGC(5/10)
Hin1I 958 GACGCC G[GA]^CG[CT]C
HincII 1698 GTTAAC GT[CT]^[GA]AC
HindII 1698 GTTAAC GT[CT]^[GA]AC
HpaI 1698 GTTAAC GTT^AAC
MvnI 88 CGCG CG^CG
NciI 1089 CCGGG CC^[GC]GG
NdeI 2510 CATATG CA^TATG
PflFI 2374 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2075 CCATGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2336 AACGTT AA^CGTT
RcaI 794 TCATGA T^CATGA
SacI 2206 GAGCTC GAGCT^C
SapI 2208 GCTCTTC GCTCTTC(1/4)
SspBI 167 TGTACA T^GTACA
Tth111I 2374 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2075 CCATGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1062 TCTAGA T^CTAGA
XcmI 2312 CCAAGATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 510 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BalI 2072 TGGCCA TGG^CCA
2496 TGGCCA
BanII 757 GGGCTC G[GA]GC[CT]^C
2206 GAGCTC
BmyI 757 GGGCTC G[AGT]GC[ACT]^C
2206 GAGCTC
BsaI 1930 GAGACC GGTCTC(1/5)
2224 GAGACC
Bsp1286I 757 GGGCTC G[AGT]GC[ACT]^C
2206 GAGCTC
BspLU11I 164 ACATGT A^CATGT
2408 ACATGT
BstXI 606 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2312 CCAAGATCCTGG
CfoI 94 GCGC GCG^C
1654 GCGC
EarI 1219 CTCTTC CTCTTC(1/4)
2209 CTCTTC
EcoNI 65 CCTTAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1410 CCTGTGCGAGG
HhaI 94 GCGC GCG^C
1654 GCGC
HinP1I 94 GCGC G^CGC
1654 GCGC
Ksp632I 1219 CTCTTC CTCTTC(1/4)
2209 CTCTTC
MluNI 2072 TGGCCA TGG^CCA
2496 TGGCCA
MscI 2072 TGGCCA TGG^CCA
2496 TGGCCA
SmlI 989 CTCAAG C^T[CT][GA]AG
1705 CTTGAG
StuI 2129 AGGCCT AGG^CCT
2440 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BbvI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DrdI DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII