(Circular) (Six-base) MAPSORT of: pFN21AE3769.seq Check: 7911 from: 1 to: 7453 pFN21AE3769 7453 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2801 2801 Size: 7453 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5263 278 Size: 53 83 186 936 3727 2468 Fragments arranged by size: 3727 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3609 4796 1937 Size: 1672 1187 4594 Fragments arranged by size: 4594 1672 1187 Acc65I G'GTAC_C Cuts at: 4774 4774 Size: 7453 AclI AA'CG_TT Cuts at: 1563 4342 4752 5580 5953 1563 Size: 2779 410 828 373 3063 Fragments arranged by size: 3063 2779 828 410 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 3143 3598 3973 5516 6900 7168 1632 Size: 812 699 455 375 1543 1384 268 1917 Fragments arranged by size: 1917 1543 1384 812 699 455 375 268 AfeI AGC'GCT Cuts at: 3660 3660 Size: 7453 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7256 AflIII A'CryG_T Cuts at: 1203 2169 4413 4566 6352 7329 1203 Size: 966 2244 153 1786 977 1327 Fragments arranged by size: 2244 1786 1327 977 966 153 AgeI A'CCGG_T Cuts at: 6284 6284 Size: 7453 AhdI GACnn_n'nnGTC Cuts at: 4306 6182 4306 Size: 1876 5577 AleI CACnn'nnGTG Cuts at: 1166 2453 3575 1166 Size: 1287 1122 5044 Fragments arranged by size: 5044 1287 1122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7453 AlwNI CAG_nnn'CTG Cuts at: 2237 3052 4362 5196 6768 2237 Size: 815 1310 834 1572 2922 Fragments arranged by size: 2922 1572 1310 834 815 ApaLI G'TGCA_C Cuts at: 5510 6666 5510 Size: 1156 6297 ApoI r'AATT_y Cuts at: 1493 3498 4024 4391 4640 4762 4988 5140 7249 Size: 2005 526 367 249 122 226 152 2109 Cuts at: 7249 1493 Size: 1697 Fragments arranged by size: 2109 2005 1697 526 367 249 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 6007 160 Size: 872 4975 1606 Fragments arranged by size: 4975 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7453 AvaI C'yCGr_G Cuts at: 1870 1943 3537 4778 1870 Size: 73 1594 1241 4545 Fragments arranged by size: 4545 1594 1241 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2317 2350 2317 Size: 33 7420 BamHI G'GATC_C Cuts at: 4783 5150 4783 Size: 367 7086 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2974 4774 6229 618 Size: 325 217 627 1187 1800 1455 1842 Fragments arranged by size: 1842 1800 1455 1187 627 325 217 BanII G_rGCy'C Cuts at: 1391 2766 4215 4772 1391 Size: 1375 1449 557 4072 Fragments arranged by size: 4072 1449 1375 557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4187 928 Size: 971 2288 4194 Fragments arranged by size: 4194 2288 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4448 4482 5644 5678 1903 Size: 34 2511 34 1162 34 3678 Fragments arranged by size: 3678 2511 1162 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3202 5345 6555 3202 Size: 2143 1210 4100 Fragments arranged by size: 4100 2143 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2972 6064 136 Size: 107 122 71 631 605 1300 3092 1525 Fragments arranged by size: 3092 1525 1300 631 605 122 107 71 BglII A'GATC_T Cuts at: 3085 7448 3085 Size: 4363 3090 BlpI GC'TnA_GC Cuts at: 4870 4870 Size: 7453 Bme1580I G_kGCm'C Cuts at: 1790 5514 6670 1790 Size: 3724 1156 2573 Fragments arranged by size: 3724 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3924 5184 6142 Size: 416 53 83 399 24 2507 1260 958 Cuts at: 6142 442 Size: 1753 Fragments arranged by size: 2507 1753 1260 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7453 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3445 3445 Size: 7453 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3415 3802 4384 6113 1239 Size: 2176 387 582 1729 2579 Fragments arranged by size: 2579 2176 1729 582 387 Bpu10I CC'TnA_GC Cuts at: 3258 3258 Size: 7453 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2979 3731 5578 6443 6741 2979 Size: 752 1847 865 298 3691 Fragments arranged by size: 3691 1847 865 752 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3929 4223 6116 882 Size: 617 2430 294 1893 2219 Fragments arranged by size: 2430 2219 1893 617 294 BsaAI yAC'GTr Cuts at: 493 1204 5244 493 Size: 711 4040 2702 Fragments arranged by size: 4040 2702 711 BsaBI GATnn'nnATC Cuts at: 7447 7447 Size: 7453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2964 5260 5642 6277 Size: 53 83 186 936 1431 2296 382 635 Cuts at: 6277 275 Size: 1451 Fragments arranged by size: 2296 1451 1431 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2035 5886 6284 6558 6705 7116 2035 Size: 3851 398 274 147 411 2372 Fragments arranged by size: 3851 2372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3611 3641 1178 Size: 30 165 30 2208 30 4990 Fragments arranged by size: 4990 2208 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3003 1186 Size: 1817 5636 BseYI C'CCAG_C Cuts at: 2163 2618 6656 7152 2163 Size: 455 4038 496 2464 Fragments arranged by size: 4038 2464 496 455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2817 1163 Size: 573 1081 5799 Fragments arranged by size: 5799 1081 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 4903 5664 6692 1540 Size: 275 186 35 2867 761 1028 2301 Fragments arranged by size: 2867 2301 1028 761 275 186 35 BsiHKAI G_wGCw'C Cuts at: 4215 5514 5599 6670 4215 Size: 1299 85 1071 4998 Fragments arranged by size: 4998 1299 1071 85 BsmI GAATG_Cn' Cuts at: 4964 5057 4964 Size: 93 7360 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2766 4215 4772 5514 5599 6670 1391 Size: 399 976 1449 557 742 85 1071 2174 Fragments arranged by size: 2174 1449 1071 976 742 557 399 85 BspEI T'CCGG_A Cuts at: 7116 7116 Size: 7453 BspHI T'CATG_A Cuts at: 2799 5342 7072 2799 Size: 2543 1730 3180 Fragments arranged by size: 3180 2543 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 4808 844 Size: 1957 2007 3489 Fragments arranged by size: 3489 2007 1957 BsrBI CCG'CTC Cuts at: 1394 1823 5340 1394 Size: 429 3517 3507 Fragments arranged by size: 3517 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 3444 3817 4464 5948 6122 65 Size: 1183 1239 957 373 647 1484 174 1396 Fragments arranged by size: 1484 1396 1239 1183 957 647 373 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 6097 6284 1700 Size: 110 311 3976 187 2869 Fragments arranged by size: 3976 2869 311 187 110 BsrGI T'GTAC_A Cuts at: 96 2172 96 Size: 2076 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7453 BssSI C'ACGA_G Cuts at: 5513 6525 5513 Size: 1012 6441 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 794 Size: 1895 5558 BstBI TT'CG_AA Cuts at: 6257 6257 Size: 7453 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7453 BstXI CCAn_nnnn'nTGG Cuts at: 2618 4324 2618 Size: 1706 5747 BstYI r'GATC_y Cuts at: 1972 2233 2702 3085 4320 4783 5150 5535 5552 Size: 261 469 383 1235 463 367 385 17 Cuts at: 5552 7004 7090 7102 7448 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1235 469 463 385 383 367 346 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3217 3576 4080 4224 4491 513 Size: 552 939 1213 359 504 144 267 3475 Fragments arranged by size: 3475 1213 939 552 504 359 267 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6714 Fragments arranged by size: 6714 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2141 2392 2822 2829 3302 3358 3907 4509 Size: 33 251 430 7 473 56 549 602 Cuts at: 4509 4969 5763 5783 2108 Size: 460 794 20 3778 Fragments arranged by size: 3778 794 602 549 473 460 430 251 56 33 20 7 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7453 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3291 3326 529 Size: 35 2727 35 4656 Fragments arranged by size: 4656 2727 35 35 DraI TTT'AAA Cuts at: 4757 4757 Size: 7453 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2053 2606 2692 6460 809 Size: 507 737 553 86 3768 1802 Fragments arranged by size: 3768 1802 737 553 507 86 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3140 4077 4501 Size: 54 1425 50 275 18 1310 937 424 Cuts at: 4501 4900 5789 7418 8 Size: 399 889 1629 43 Fragments arranged by size: 1629 1425 1310 937 889 424 399 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4900 1537 Size: 275 3088 4090 Fragments arranged by size: 4090 3088 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3230 4220 5383 1353 Size: 1877 990 1163 3423 Fragments arranged by size: 3423 1877 1163 990 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 6040 6414 6560 387 Size: 1064 138 435 4016 374 146 1280 Fragments arranged by size: 4016 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 4213 4213 Size: 7453 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 3143 3415 3598 3802 3973 4384 Size: 393 812 699 272 183 204 171 411 Cuts at: 4384 5516 6113 6900 7168 1239 Size: 1132 597 787 268 1524 Fragments arranged by size: 1524 1132 812 787 699 597 411 393 272 268 204 183 171 EcoNI CCTnn'n_nnAGG Cuts at: 2074 3419 2074 Size: 1345 6108 EcoO109I rG'GnC_Cy Cuts at: 3804 3804 Size: 7453 EcoRI G'AATT_C Cuts at: 4391 4762 5140 4391 Size: 371 378 6704 Fragments arranged by size: 6704 378 371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3130 3899 3130 Size: 769 6684 FspI TGC'GCA Cuts at: 5959 5959 Size: 7453 HaeII r_GCGC'y Cuts at: 1678 3662 6600 1678 Size: 1984 2938 2531 Fragments arranged by size: 2938 2531 1984 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2726 2758 3611 3643 4112 Size: 32 135 32 1154 32 853 32 469 Cuts at: 4112 4144 4191 4223 4292 4324 6094 6126 6168 Size: 32 47 32 69 32 1770 32 42 Cuts at: 6168 6200 1373 Size: 32 2626 Fragments arranged by size: 2626 1770 1154 853 469 135 69 47 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3705 4797 5043 669 Size: 988 281 1767 1092 246 3079 Fragments arranged by size: 3079 1767 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7453 HpaI GTT'AAC Cuts at: 3705 5043 3705 Size: 1338 6115 KpnI G_GTAC'C Cuts at: 4778 4778 Size: 7453 MfeI C'AATT_G Cuts at: 5052 5052 Size: 7453 MluI A'CGCG_T Cuts at: 7329 7329 Size: 7453 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 3435 6567 6751 1305 Size: 808 1322 3132 184 2007 Fragments arranged by size: 3132 2007 1322 808 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4079 4503 10 Size: 54 1425 343 2247 424 2960 Fragments arranged by size: 2960 2247 1425 424 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3551 3575 4223 4469 5411 5770 Size: 648 1287 1098 24 648 246 942 359 Cuts at: 5770 5929 518 Size: 159 2042 Fragments arranged by size: 2042 1287 1098 942 648 648 359 246 159 24 MspA1I CmG'CkG Cuts at: 1606 2163 3394 3828 4016 4037 4869 5546 6694 Size: 557 1231 434 188 21 832 677 1148 Cuts at: 6694 6939 1606 Size: 245 2120 Fragments arranged by size: 2120 1231 1148 832 677 557 434 245 188 21 NcoI C'CATG_G Cuts at: 513 1065 2004 3217 4080 4224 4491 513 Size: 552 939 1213 863 144 267 3475 Fragments arranged by size: 3475 1213 939 863 552 267 144 NdeI CA'TA_TG Cuts at: 387 4516 387 Size: 4129 3324 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7453 NotI GC'GGCC_GC Cuts at: 4900 4900 Size: 7453 NspI r_CATG'y Cuts at: 1132 1229 2173 2986 4151 4159 4417 4811 6356 Size: 97 944 813 1165 8 258 394 1545 Cuts at: 6356 1132 Size: 2229 Fragments arranged by size: 2229 1545 1165 944 813 394 258 97 8 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7453 PciI A'CATG_T Cuts at: 2169 4413 6352 2169 Size: 2244 1939 3270 Fragments arranged by size: 3270 2244 1939 PflMI CCAn_nnn'nTGG Cuts at: 1108 4086 1108 Size: 2978 4475 PfoI T'CCnGG_A Cuts at: 2610 3818 2610 Size: 1208 6245 PmeI GTTT'AAAC Cuts at: 4757 4757 Size: 7453 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2412 2444 5522 5554 7035 7067 2412 Size: 32 3078 32 1481 32 2798 Fragments arranged by size: 3078 2798 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7453 PsiI TTA'TAA Cuts at: 4647 5023 4647 Size: 376 7077 PstI C_TGCA'G Cuts at: 830 3365 3454 3569 4805 830 Size: 2535 89 115 1236 3478 Fragments arranged by size: 3478 2535 1236 115 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7453 PvuII CAG'CTG Cuts at: 2163 3828 4016 4037 2163 Size: 1665 188 21 5579 Fragments arranged by size: 5579 1665 188 21 SacI G_AGCT'C Cuts at: 4215 4215 Size: 7453 SalI G'TCGA_C Cuts at: 1936 4795 1936 Size: 2859 4594 SapI GCTCTTCn'nnn_ Cuts at: 4220 4220 Size: 7453 SbfI CC_TGCA'GG Cuts at: 4805 4805 Size: 7453 ScaI AGT'ACT Cuts at: 5701 7413 5701 Size: 1712 5741 SfcI C'TryA_G Cuts at: 826 1045 3361 3450 3565 4801 5936 6617 6808 Size: 219 2316 89 115 1236 1135 681 191 Cuts at: 6808 826 Size: 1471 Fragments arranged by size: 2316 1471 1236 1135 681 219 191 115 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7453 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7453 SmaI CCC'GGG Cuts at: 1872 4780 1872 Size: 2908 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2994 3710 5557 6458 6720 820 Size: 197 926 1051 716 1847 901 262 1553 Fragments arranged by size: 1847 1553 1051 926 901 716 262 197 SnaBI TAC'GTA Cuts at: 493 5244 493 Size: 4751 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7453 SphI G_CATG'C Cuts at: 1132 4811 1132 Size: 3679 3774 SspI AAT'ATT Cuts at: 5 52 5377 5 Size: 47 5325 2081 Fragments arranged by size: 5325 2081 47 StuI AGG'CCT Cuts at: 1517 4136 4447 1517 Size: 2619 311 4523 Fragments arranged by size: 4523 2619 311 StyI C'CwwG_G Cuts at: 513 1065 2004 3217 3792 4080 4224 4234 4491 Size: 552 939 1213 575 288 144 10 257 Cuts at: 4491 4892 513 Size: 401 3074 Fragments arranged by size: 3074 1213 939 575 552 401 288 257 144 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 3956 5480 5497 5650 5835 6280 Size: 1255 591 1785 1524 17 153 185 445 Cuts at: 6280 325 Size: 1498 Fragments arranged by size: 1785 1524 1498 1255 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 5699 7411 Size: 274 80 33 51 1443 195 3527 1712 Cuts at: 7411 96 Size: 138 Fragments arranged by size: 3527 1712 1443 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3814 3846 7413 7445 3814 Size: 32 3567 32 3822 Fragments arranged by size: 3822 3567 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4382 1316 Size: 3066 4387 XbaI T'CTAG_A Cuts at: 3067 4789 3067 Size: 1722 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4324 4324 Size: 7453 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7453 XmaI C'CCGG_G Cuts at: 1870 4778 1870 Size: 2908 4545 XmnI GAAnn'nnTTC Cuts at: 2519 5582 2519 Size: 3063 4390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5261 276 Size: 53 83 186 936 3727 2468 Fragments arranged by size: 3727 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI