(Linear) (Six-base) MAPSORT of: KIEE3769.seq Check: 2486 from: 1 to: 2748 KIEE3769.orf With 182 enzymes: SgfI * July 3, 2012 18:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 796 2748 Size: 796 1952 AccI GT'mk_AC Cuts at: 0 1604 2748 Size: 1604 1144 AclI AA'CG_TT Cuts at: 0 2337 2748 Size: 2337 411 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 1138 1593 1968 2748 Size: 439 699 455 375 780 Fragments arranged by size: 780 699 455 439 375 AfeI AGC'GCT Cuts at: 0 1655 2748 Size: 1655 1093 AflIII A'CryG_T Cuts at: 0 164 2408 2561 2748 Size: 164 2244 153 187 Fragments arranged by size: 2244 187 164 153 AhdI GACnn_n'nnGTC Cuts at: 0 2301 2748 Size: 2301 447 AleI CACnn'nnGTG Cuts at: 0 448 1570 2748 Size: 448 1122 1178 Fragments arranged by size: 1178 1122 448 AlwNI CAG_nnn'CTG Cuts at: 0 232 1047 2357 2748 Size: 232 815 1310 391 Fragments arranged by size: 1310 815 391 232 ApoI r'AATT_y Cuts at: 0 1493 2019 2386 2635 2748 Size: 1493 526 367 249 113 Fragments arranged by size: 1493 526 367 249 113 AvaI C'yCGr_G Cuts at: 0 1532 2748 Size: 1532 1216 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 312 345 2748 Size: 312 33 2403 Fragments arranged by size: 2403 312 33 BanI G'GyrC_C Cuts at: 0 969 2748 Size: 969 1779 BanII G_rGCy'C Cuts at: 0 761 2210 2748 Size: 761 1449 538 Fragments arranged by size: 1449 761 538 BbsI GAAGACnn'nnnn_ Cuts at: 0 2182 2748 Size: 2182 566 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2443 2477 2748 Size: 2443 34 271 Fragments arranged by size: 2443 271 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1197 2748 Size: 1197 1551 BglI GCCn_nnn'nGGC Cuts at: 0 967 2748 Size: 967 1781 BglII A'GATC_T Cuts at: 0 1080 2748 Size: 1080 1668 BmrI ACTGGGnnnn_n' Cuts at: 0 1919 2748 Size: 1919 829 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1440 2748 Size: 1440 1308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1410 1797 2379 2748 Size: 1410 387 582 369 Fragments arranged by size: 1410 582 387 369 Bpu10I CC'TnA_GC Cuts at: 0 1253 2748 Size: 1253 1495 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 974 1726 2748 Size: 974 752 1022 Fragments arranged by size: 1022 974 752 BsaI GGTCTCn'nnnn_ Cuts at: 0 1924 2218 2748 Size: 1924 294 530 Fragments arranged by size: 1924 530 294 BsaHI Gr'CG_yC Cuts at: 0 959 2748 Size: 959 1789 BsaWI w'CCGG_w Cuts at: 0 30 2748 Size: 30 2718 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1606 1636 2748 Size: 1606 30 1112 Fragments arranged by size: 1606 1112 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 998 2748 Size: 998 1750 BseYI C'CCAG_C Cuts at: 0 158 613 2748 Size: 158 455 2135 Fragments arranged by size: 2135 455 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 812 2748 Size: 812 1936 BsiEI CG_ry'CG Cuts at: 0 31 2748 Size: 31 2717 BsiHKAI G_wGCw'C Cuts at: 0 2210 2748 Size: 2210 538 Bsp1286I G_dGCh'C Cuts at: 0 761 2210 2748 Size: 761 1449 538 Fragments arranged by size: 1449 761 538 BspHI T'CATG_A Cuts at: 0 794 2748 Size: 794 1954 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 796 2748 Size: 796 1952 BsrDI GCAATG_nn' Cuts at: 0 482 1439 1812 2459 2748 Size: 482 957 373 647 289 Fragments arranged by size: 957 647 482 373 289 BsrFI r'CCGG_y Cuts at: 0 116 2748 Size: 116 2632 BsrGI T'GTAC_A Cuts at: 0 167 2748 Size: 167 2581 BstAPI GCAn_nnn'nTGC Cuts at: 0 684 2748 Size: 684 2064 BstXI CCAn_nnnn'nTGG Cuts at: 0 613 2319 2748 Size: 613 1706 429 Fragments arranged by size: 1706 613 429 BstYI r'GATC_y Cuts at: 0 228 697 1080 2315 2748 Size: 228 469 383 1235 433 Fragments arranged by size: 1235 469 433 383 228 BtgI C'CryG_G Cuts at: 0 1212 1571 2075 2219 2486 2748 Size: 1212 359 504 144 267 262 Fragments arranged by size: 1212 504 359 267 262 144 BtsI GCAGTG_nn' Cuts at: 0 103 136 387 817 824 1297 1353 1902 Size: 103 33 251 430 7 473 56 549 Cuts at: 1902 2504 2748 Size: 602 244 Fragments arranged by size: 602 549 473 430 251 244 103 56 33 7 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1286 1321 2748 Size: 1286 35 1427 Fragments arranged by size: 1427 1286 35 DrdI GACnn_nn'nnGTC Cuts at: 0 48 601 687 2748 Size: 48 553 86 2061 Fragments arranged by size: 2061 553 86 48 EaeI y'GGCC_r Cuts at: 0 1135 2072 2496 2748 Size: 1135 937 424 252 Fragments arranged by size: 1135 937 424 252 EarI CTCTTCn'nnn_ Cuts at: 0 1225 2215 2748 Size: 1225 990 533 Fragments arranged by size: 1225 990 533 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 2748 Size: 19 2729 EcoICRI GAG'CTC Cuts at: 0 2208 2748 Size: 2208 540 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 1138 1410 1593 1797 1968 2379 2748 Size: 439 699 272 183 204 171 411 369 Fragments arranged by size: 699 439 411 369 272 204 183 171 EcoNI CCTnn'n_nnAGG Cuts at: 0 69 1414 2748 Size: 69 1345 1334 Fragments arranged by size: 1345 1334 69 EcoO109I rG'GnC_Cy Cuts at: 0 1799 2748 Size: 1799 949 EcoRI G'AATT_C Cuts at: 0 2386 2748 Size: 2386 362 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1125 1894 2748 Size: 1125 769 854 Fragments arranged by size: 1125 854 769 HaeII r_GCGC'y Cuts at: 0 1657 2748 Size: 1657 1091 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 721 753 1606 1638 2107 2139 2186 2218 Size: 721 32 853 32 469 32 47 32 Cuts at: 2218 2287 2319 2748 Size: 69 32 429 Fragments arranged by size: 853 721 469 429 69 47 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1700 2748 Size: 1700 1048 HpaI GTT'AAC Cuts at: 0 1700 2748 Size: 1700 1048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 108 1430 2748 Size: 108 1322 1318 Fragments arranged by size: 1322 1318 108 MscI TGG'CCA Cuts at: 0 2074 2498 2748 Size: 2074 424 250 Fragments arranged by size: 2074 424 250 MslI CAynn'nnrTG Cuts at: 0 448 1546 1570 2218 2464 2748 Size: 448 1098 24 648 246 284 Fragments arranged by size: 1098 648 448 284 246 24 MspA1I CmG'CkG Cuts at: 0 158 1389 1823 2011 2032 2748 Size: 158 1231 434 188 21 716 Fragments arranged by size: 1231 716 434 188 158 21 NcoI C'CATG_G Cuts at: 0 1212 2075 2219 2486 2748 Size: 1212 863 144 267 262 Fragments arranged by size: 1212 863 267 262 144 NdeI CA'TA_TG Cuts at: 0 2511 2748 Size: 2511 237 NspI r_CATG'y Cuts at: 0 168 981 2146 2154 2412 2748 Size: 168 813 1165 8 258 336 Fragments arranged by size: 1165 813 336 258 168 8 PciI A'CATG_T Cuts at: 0 164 2408 2748 Size: 164 2244 340 Fragments arranged by size: 2244 340 164 PflMI CCAn_nnn'nTGG Cuts at: 0 2081 2748 Size: 2081 667 PfoI T'CCnGG_A Cuts at: 0 605 1813 2748 Size: 605 1208 935 Fragments arranged by size: 1208 935 605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 407 439 2748 Size: 407 32 2309 Fragments arranged by size: 2309 407 32 PsiI TTA'TAA Cuts at: 0 2642 2748 Size: 2642 106 PstI C_TGCA'G Cuts at: 0 1360 1449 1564 2748 Size: 1360 89 115 1184 Fragments arranged by size: 1360 1184 115 89 PvuII CAG'CTG Cuts at: 0 158 1823 2011 2032 2748 Size: 158 1665 188 21 716 Fragments arranged by size: 1665 716 188 158 21 SacI G_AGCT'C Cuts at: 0 2210 2748 Size: 2210 538 SapI GCTCTTCn'nnn_ Cuts at: 0 2215 2748 Size: 2215 533 SfcI C'TryA_G Cuts at: 0 1356 1445 1560 2748 Size: 1356 89 115 1188 Fragments arranged by size: 1356 1188 115 89 SmlI C'TyrA_G Cuts at: 0 989 1705 2748 Size: 989 716 1043 Fragments arranged by size: 1043 989 716 StuI AGG'CCT Cuts at: 0 2131 2442 2748 Size: 2131 311 306 Fragments arranged by size: 2131 311 306 StyI C'CwwG_G Cuts at: 0 1212 1787 2075 2219 2229 2486 2748 Size: 1212 575 288 144 10 257 262 Fragments arranged by size: 1212 575 288 262 257 144 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 166 1951 2748 Size: 166 1785 797 Fragments arranged by size: 1785 797 166 TatI w'GTAC_w Cuts at: 0 167 2748 Size: 167 2581 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1809 1841 2748 Size: 1809 32 907 Fragments arranged by size: 1809 907 32 Tth111I GACn'n_nGTC Cuts at: 0 2377 2748 Size: 2377 371 XbaI T'CTAG_A Cuts at: 0 1062 2748 Size: 1062 1686 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2319 2748 Size: 2319 429 XmnI GAAnn'nnTTC Cuts at: 0 514 2748 Size: 514 2234 Enzymes that do cut: AarI AccI AclI AcuI AfeI AflIII AhdI AleI AlwNI ApoI AvaI BaeI BanI BanII BbsI BcgI BciVI BglI BglII BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI PpiI PsiI PstI PvuII SacI SapI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AflII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BclI BfrBI BlpI Bme1580I BmgBI BmtI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XhoI XmaI ZraI